Enhancing glycan occupancy of soluble HIV-1 envelope trimers to mimic the native viral spike

Immunofocusing and enhancing autologous Tier-2 HIV-1 neutralization by displaying Env trimers on two-component protein nanoparticles

(2021) Philip J. M. Brouwer et al.   npj Vaccines

Env Exceptionalism: Why Are HIV-1 Env Glycoproteins Atypical Immunogens?

(2020) P.J. Klasse et al.   Cell Host & Microbe

Mapping Polyclonal Antibody Responses in Non-human Primates Vaccinated with HIV Env Trimer Subunit Vaccines

(2020) Bartek Nogal et al.   Cell Reports

Neutralizing antibody responses induced by HIV-1 envelope glycoprotein SOSIP trimers derived from elite neutralizers

(2020) Anna Schorcht et al.   JOURNAL OF VIROLOGY

Mapping the immunogenic landscape of near-native HIV-1 envelope trimers in non-human primates

(2020) Christopher A. Cottrell et al.   PLoS Pathogens

Stabilization of the V2 loop improves the presentation of V2 loop–associated broadly neutralizing antibody epitopes on HIV-1 envelope trimers

(2019) Steven W. de Taeye et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Protein and Glycan Mimicry in HIV Vaccine Design

(2019) Gemma E. Seabright et al.   JOURNAL OF MOLECULAR BIOLOGY

Immunization expands B cells specific to HIV-1 V3 glycan in mice and macaques

(2019) Amelia Escolano et al.   NATURE

Lower Broadly Neutralizing Antibody Responses in Female Versus Male HIV-1 Infected Injecting Drug Users

(2019) Zelda EULER et al.   Viruses-Basel

Optimizing the production and affinity purification of HIV-1 envelope glycoprotein SOSIP trimers from transiently transfected CHO cells

(2019) Albert Cupo et al.   PLoS One

Structure and immunogenicity of a stabilized HIV-1 envelope trimer based on a group-M consensus sequence

(2019) Kwinten Sliepen et al.   Nature Communications

Similarities and differences between native HIV-1 envelope glycoprotein trimers and stabilized soluble trimer mimetics

(2019) Alba Torrents de la Peña et al.   PLoS Pathogens

Structure and Immune Recognition of the HIV Glycan Shield

(2018) Max Crispin et al.   Annual Review of Biophysics

Immunogenicity in Rabbits of HIV-1 SOSIP Trimers from Clades A, B, and C, Given Individually, Sequentially, or in Combination

(2018) Alba Torrents de la Peña et al.   JOURNAL OF VIROLOGY

Epitopes for neutralizing antibodies induced by HIV-1 envelope glycoprotein BG505 SOSIP trimers in rabbits and macaques

(2018) P. J. Klasse et al.   PLoS Pathogens

Glycan Masking Focuses Immune Responses to the HIV-1 CD4-Binding Site and Enhances Elicitation of VRC01-Class Precursor Antibodies

(2018) Hongying Duan et al.   IMMUNITY

Differential processing of HIV envelope glycans on the virus and soluble recombinant trimer

(2018) Liwei Cao et al.   Nature Communications

Site-Specific Glycosylation of Virion-Derived HIV-1 Env Is Mimicked by a Soluble Trimeric Immunogen

(2018) Weston B. Struwe et al.   Cell Reports

Closing and opening holes in the glycan shield of HIV-1 envelope glycoprotein SOSIP trimers can redirect the neutralizing antibody response to the newly unmasked epitopes

(2018) Rajesh P. Ringe et al.   JOURNAL OF VIROLOGY

Completeness of HIV-1 Envelope Glycan Shield at Transmission Determines Neutralization Breadth

(2018) Kshitij Wagh et al.   Cell Reports

A Broadly Neutralizing Antibody Targets the Dynamic HIV Envelope Trimer Apex via a Long, Rigidified, and Anionic β-Hairpin Structure

(2017) Jeong Hyun Lee et al.   IMMUNITY

Characterization of a stable HIV-1 B/C recombinant, soluble, and trimeric envelope glycoprotein (Env) highly resistant to CD4-induced conformational changes

(2017) Rajesh Kumar et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Glycosylation Benchmark Profile for HIV-1 Envelope Glycoprotein Production Based on Eleven Env Trimers

(2017) Eden P. Go et al.   JOURNAL OF VIROLOGY

cryoSPARC: algorithms for rapid unsupervised cryo-EM structure determination

(2017) Ali Punjani et al.   NATURE METHODS

Global site-specific N-glycosylation analysis of HIV envelope glycoprotein

(2017) Liwei Cao et al.   Nature Communications

Improving the Immunogenicity of Native-like HIV-1 Envelope Trimers by Hyperstabilization

(2017) Alba Torrents de la Peña et al.   Cell Reports

Quantification of the Impact of the HIV-1-Glycan Shield on Antibody Elicitation

(2017) Tongqing Zhou et al.   Cell Reports

Structure and topology around the cleavage site regulate post-translational cleavage of the HIV-1 gp160 signal peptide

(2017) Erik Lee Snapp et al.   eLife

N-linked glycosylation and homeostasis of the endoplasmic reticulum

(2016) Natalia Cherepanova et al.   CURRENT OPINION IN CELL BIOLOGY

HIV Vaccine Design to Target Germline Precursors of Glycan-Dependent Broadly Neutralizing Antibodies

(2016) Jon M. Steichen et al.   IMMUNITY

Composition and Antigenic Effects of Individual Glycan Sites of a Trimeric HIV-1 Envelope Glycoprotein

(2016) Anna-Janina Behrens et al.   Cell Reports

Holes in the Glycan Shield of the Native HIV Envelope Are a Target of Trimer-Elicited Neutralizing Antibodies

(2016) Laura E. McCoy et al.   Cell Reports

Sequential and Simultaneous Immunization of Rabbits with HIV-1 Envelope Glycoprotein SOSIP.664 Trimers from Clades A, B and C

(2016) P. J. Klasse et al.   PLoS Pathogens

An HIV-1 antibody from an elite neutralizer implicates the fusion peptide as a site of vulnerability

(2016) Marit J. van Gils et al.   Nature Microbiology

Immunogenicity of Stabilized HIV-1 Envelope Trimers with Reduced Exposure of Non-neutralizing Epitopes

(2015) Steven W. de Taeye et al.   CELL

Human cell lines for biopharmaceutical manufacturing: history, status, and future perspectives

(2015) Jennifer Dumont et al.   CRITICAL REVIEWS IN BIOTECHNOLOGY

Cell- and Protein-Directed Glycosylation of Native Cleaved HIV-1 Envelope

(2015) Laura K. Pritchard et al.   JOURNAL OF VIROLOGY

A Native-Like SOSIP.664 Trimer Based on an HIV-1 Subtype BenvGene

(2015) Pavel Pugach et al.   JOURNAL OF VIROLOGY

Comparative Analysis of the Glycosylation Profiles of Membrane-Anchored HIV-1 Envelope Glycoprotein Trimers and Soluble gp140

(2015) Eden P. Go et al.   JOURNAL OF VIROLOGY

Murine Antibody Responses to Cleaved Soluble HIV-1 Envelope Trimers Are Highly Restricted in Specificity

(2015) Joyce K. Hu et al.   JOURNAL OF VIROLOGY

Design and structure of two HIV-1 clade C SOSIP.664 trimers that increase the arsenal of native-like Env immunogens

(2015) Jean-Philippe Julien et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

HIV-1 neutralizing antibodies induced by native-like envelope trimers

(2015) R. W. Sanders et al.   SCIENCE

Glycan clustering stabilizes the mannose patch of HIV-1 and preserves vulnerability to broadly neutralizing antibodies

(2015) Laura K. Pritchard et al.   Nature Communications

Structural Constraints Determine the Glycosylation of HIV-1 Envelope Trimers

(2015) Laura K. Pritchard et al.   Cell Reports

Structural Delineation of a Quaternary, Cleavage-Dependent Epitope at the gp41-gp120 Interface on Intact HIV-1 Env Trimers

(2014) Claudia Blattner et al.   IMMUNITY

Broad and potent HIV-1 neutralization by a human antibody that binds the gp41–gp120 interface

(2014) Jinghe Huang et al.   NATURE

Recombinant HIV envelope trimer selects for quaternary-dependent antibodies targeting the trimer apex

(2014) Devin Sok et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Differential binding of neutralizing and non-neutralizing antibodies to native-like soluble HIV-1 Env trimers, uncleaved Env proteins, and monomeric subunits

(2014) Anila Yasmeen et al.   Retrovirology

CD4-Induced Activation in a Soluble HIV-1 Env Trimer

(2014) Miklos Guttman et al.   STRUCTURE

Mechanism of Bacterial Oligosaccharyltransferase

(2013) Sabina Gerber et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Glycosylation of closely spaced acceptor sites in human glycoproteins

(2013) S. Shrimal et al.   JOURNAL OF CELL SCIENCE

Asymmetric recognition of the HIV-1 trimer by broadly neutralizing antibody PG9

(2013) Jean-Philippe Julien et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Crystal Structure of a Soluble Cleaved HIV-1 Envelope Trimer

(2013) J.-P. Julien et al.   SCIENCE

Cryo-EM Structure of a Fully Glycosylated Soluble Cleaved HIV-1 Envelope Trimer

(2013) D. Lyumkis et al.   SCIENCE

A Next-Generation Cleaved, Soluble HIV-1 Env Trimer, BG505 SOSIP.664 gp140, Expresses Multiple Epitopes for Broadly Neutralizing but Not Non-Neutralizing Antibodies

(2013) Rogier W. Sanders et al.   PLoS Pathogens

Broad neutralization by a combination of antibodies recognizing the CD4 binding site and a new conformational epitope on the HIV-1 envelope protein

(2012) Florian Klein et al.   JOURNAL OF EXPERIMENTAL MEDICINE

Analysis of a Clonal Lineage of HIV-1 Envelope V2/V3 Conformational Epitope-Specific Broadly Neutralizing Antibodies and Their Inferred Unmutated Common Ancestors

(2011) M. Bonsignori et al.   JOURNAL OF VIROLOGY

Broad neutralization coverage of HIV by multiple highly potent antibodies

(2011) Laura M. Walker et al.   NATURE

The Glycan Shield of HIV Is Predominantly Oligomannose Independently of Production System or Viral Clade

(2011) Camille Bonomelli et al.   PLoS One

Sequence and Structural Convergence of Broad and Potent HIV Antibodies That Mimic CD4 Binding

(2011) J. F. Scheid et al.   SCIENCE

Envelope glycans of immunodeficiency virions are almost entirely oligomannose antigens

(2010) K. J. Doores et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Rational Design of Envelope Identifies Broadly Neutralizing Human Monoclonal Antibodies to HIV-1

(2010) X. Wu et al.   SCIENCE

Cotranslational and Posttranslational N-Glycosylation of Polypeptides by Distinct Mammalian OST Isoforms

(2009) Catalina Ruiz-Canada et al.   CELL

Detailed Mechanistic Insights into HIV-1 Sensitivity to Three Generations of Fusion Inhibitors

(2009) Dirk Eggink et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Improving the expression of recombinant soluble HIV Envelope glycoproteins using pseudo-stable transient transfection

(2009) George Sellhorn et al.   VACCINE

Optimization of Human Immunodeficiency Virus Type 1 Envelope Glycoproteins with V1/V2 Deleted, Using Virus Evolution

(2008) I. Bontjer et al.   JOURNAL OF VIROLOGY