Control of Multigene Expression Stoichiometry in Mammalian Cells Using Synthetic Promoters

CHO genome mining for synthetic promoter design

(2019) Yusuf B. Johari et al.   JOURNAL OF BIOTECHNOLOGY

Translation affects mRNA stability in a codon-dependent manner in human cells

(2019) Qiushuang Wu et al.   eLife

mRNA structure regulates protein expression through changes in functional half-life

(2019) David M. Mauger et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Mobius Assembly: A versatile Golden-Gate framework towards universal DNA assembly

(2018) Andreas I. Andreou et al.   PLoS One

Programming gene and engineered-cell therapies with synthetic biology

(2018) Tasuku Kitada et al.   SCIENCE

Functional Characterization of Insulation Effect for Synthetic Gene Circuits in Mammalian Cells

(2018) Weixi Liao et al.   ACS Synthetic Biology

Single Day Construction of Multigene Circuits with 3G Assembly

(2018) Andrew D. Halleran et al.   ACS Synthetic Biology

Optimization of viral protein ratios for production of rAAV serotype 5 in the baculovirus system

(2018) Bas Bosma et al.   GENE THERAPY

Synthetic control systems for high performance gene expression in mammalian cells

(2018) Gabriele Lillacci et al.   NUCLEIC ACIDS RESEARCH

In silico design of context-responsive mammalian promoters with user-defined functionality

(2017) Adam J. Brown et al.   NUCLEIC ACIDS RESEARCH

Transcriptome-Based Identification of the Optimal Reference CHO Genes for Normalisation of qPCR Data

(2017) Adam J. Brown et al.   Biotechnology Journal

Cofactor squelching: Artifact or fact?

(2016) Søren Fisker Schmidt et al.   BIOESSAYS

Multi-level regulation of cellular glycosylation: from genes to transcript to enzyme to structure

(2016) Sriram Neelamegham et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

Transcriptional termination in mammals: Stopping the RNA polymerase II juggernaut

(2016) N. J. Proudfoot   SCIENCE

Components of Adenovirus Genome Packaging

(2016) Yadvinder S. Ahi et al.   Frontiers in Microbiology

Overcoming the Specific Toxicity of Large Plasmids Electrotransfer in Primary Cells In Vitro

(2016) Léa L Lesueur et al.   Molecular Therapy-Nucleic Acids

Effects of Circular DNA Length on Transfection Efficiency by Electroporation into HeLa Cells

(2016) Benjamin D. Hornstein et al.   PLoS One

One-step Multiplex Transgenesis via Sleeping Beauty Transposition in Cattle

(2016) Wiebke Garrels et al.   Scientific Reports

Improvement of the reverse tetracycline transactivator by single amino acid substitutions that reduce leaky target gene expression to undetectable levels

(2016) Ian J. Roney et al.   Scientific Reports

NF-κB, CRE and YY1 elements are key functional regulators of CMV promoter-driven transient gene expression in CHO cells

(2015) Adam J. Brown et al.   Biotechnology Journal

Codon Optimality Is a Major Determinant of mRNA Stability

(2015) Vladimir Presnyak et al.   CELL

Highly efficient Cas9-mediated transcriptional programming

(2015) Alejandro Chavez et al.   NATURE METHODS

NF-κB, CRE and YY1 elements are key functional regulators of CMV promoter-driven transient gene expression in CHO cells

(2015) Adam J. Brown et al.   Biotechnology Journal

Synthetic promoters for CHO cell engineering

(2014) Adam J. Brown et al.   BIOTECHNOLOGY AND BIOENGINEERING

Synthetic therapeutic gene circuits in mammalian cells

(2014) Haifeng Ye et al.   FEBS LETTERS

Recent advances in DNA assembly technologies

(2014) Ran Chao et al.   FEMS YEAST RESEARCH

Synthetic biology in mammalian cells: next generation research tools and therapeutics

(2014) Florian Lienert et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

A Novel System for Simultaneous or Sequential Integration of Multiple Gene-Loading Vectors into a Defined Site of a Human Artificial Chromosome

(2014) Teruhiko Suzuki et al.   PLoS One

Transcription Activator-like Effectors: A Toolkit for Synthetic Biology

(2014) Richard Moore et al.   ACS Synthetic Biology

AAV-Mediated Gene Therapy for Research and Therapeutic Purposes

(2014) R. Jude Samulski et al.   Annual Review of Virology

Bile acid-controlled transgene expression in mammalian cells and mice

(2013) Katrin Rössger et al.   METABOLIC ENGINEERING

Rapid, modular and reliable construction of complex mammalian gene circuits

(2013) Patrick Guye et al.   NUCLEIC ACIDS RESEARCH

Synthetic mammalian trigger-controlled bipartite transcription factors

(2013) Marc Folcher et al.   NUCLEIC ACIDS RESEARCH

Rapid construction of insulated genetic circuits via synthetic sequence-guided isothermal assembly

(2013) Joseph P. Torella et al.   NUCLEIC ACIDS RESEARCH

ZFN, TALEN, and CRISPR/Cas-based methods for genome engineering

(2013) Thomas Gaj et al.   TRENDS IN BIOTECHNOLOGY

The Chicken Hypersensitivity Site 4 Core Insulator Blocks Promoter Interference in Lentiviral Vectors

(2013) Naoya Uchida et al.   Human Gene Therapy Methods

The Objectivity of Reporters: Interference Between Physically Unlinked Promoters Affects Reporter Gene Expression in Transient Transfection Experiments

(2012) Ildikó Huliák et al.   DNA AND CELL BIOLOGY

AT-rich sequence elements promote nascent transcript cleavage leading to RNA polymerase II termination

(2012) Eleanor White et al.   NUCLEIC ACIDS RESEARCH

From gene switches to mammalian designer cells: present and future prospects

(2012) Simon Ausländer et al.   TRENDS IN BIOTECHNOLOGY

Fast track assembly of multigene constructs using Golden Gate cloning and the MoClo system

(2012) Stefan Werner et al.   Bioengineered

Rational design of a small molecule-responsive intramer controlling transgene expression in mammalian cells

(2011) David Ausländer et al.   NUCLEIC ACIDS RESEARCH

A Modular Cloning System for Standardized Assembly of Multigene Constructs

(2011) Ernst Weber et al.   PLoS One

A Synthetic Optogenetic Transcription Device Enhances Blood-Glucose Homeostasis in Mice

(2011) H. Ye et al.   SCIENCE

Functional consequences of bidirectional promoters

(2011) Wu Wei et al.   TRENDS IN GENETICS

Cell line-specific control of recombinant monoclonal antibody production by CHO cells

(2010) Peter M. O'Callaghan et al.   BIOTECHNOLOGY AND BIOENGINEERING

A tunable dual-promoter integrator for targeting of cancer cells

(2010) Lior Nissim et al.   Molecular Systems Biology

Multiplexed transposon-mediated stable gene transfer in human cells

(2010) K. M. Kahlig et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

A plasmid-based multigene expression system for mammalian cells

(2010) Andrijana Kriz et al.   Nature Communications

Independent and high-level dual-gene expression in adult stem-progenitor cells from a single lentiviral vector

(2009) J Tian et al.   GENE THERAPY

Transcriptional Termination Enhances Protein Expression in Human Cells

(2009) Steven West et al.   MOLECULAR CELL

Stoichiometric and temporal requirements of Oct4, Sox2, Klf4, and c-Myc expression for efficient human iPSC induction and differentiation

(2009) E. P. Papapetrou et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Bidirectional promoter interference between two widely used internal heterologous promoters in a late-generation lentiviral construct

(2008) J A Curtin et al.   GENE THERAPY

A One Pot, One Step, Precision Cloning Method with High Throughput Capability

(2008) Carola Engler et al.   PLoS One

Divergent Transcription from Active Promoters

(2008) Amy C. Seila et al.   SCIENCE