Article
Microbiology
Laura White, Bilgi Erbay, G. Eric Blair
Summary: Cajal bodies can be reorganized into microfoci during adenovirus infection. The protein p80-coilin forms a complex with L4-22K protein, facilitating the nuclear export of Ad mRNA.
Review
Virology
Venigalla B. Rao, Andrei Fokine, Qianglin Fang, Qianqian Shao
Summary: Recent discoveries on the T4 head structure and genome packaging have provided significant insights into the biology of bacteriophage, highlighting the importance of T4 as a model organism.
Article
Biotechnology & Applied Microbiology
Jitesh Kumar, Ravi Ranjan Kumar, Dilip Kumar Das, Auroshikha Mohanty, Kumari Rajani, Namaste Kumari, Vinod Kumar, Sunil Kumar, Bajarang Vasant Kumbhar, Tushar Ranjan
Summary: A novel ATPase domain in the capsid protein of Potato leafroll virus (PLRV) has been identified, and the silencing of this domain can prevent PLRV genome packaging and assembly, providing a robust approach to generate PLRV-resistant potato plants.
Review
Virology
Shermila Kulanayake, Suresh K. Tikoo
Summary: Adenoviruses have been used as a model for investigating viral-cell interactions and discovering cellular processes, as well as for the development of viral vectors for vaccination and gene therapy. There has been increased interest in elucidating the structure and function of adenovirus core proteins in recent years.
Article
Biochemistry & Molecular Biology
Nicholas A. A. Swanson, Fenglin Li, Ruoyu Yang, Ravi K. K. Lokareddy, Gino Cingolani, Chun-Feng David Hou
Summary: In this paper, the cryo-EM structure of the portal protein from the Pseudomonas-phage PaP3 was determined, revealing a helical symmetry. The study found that the barrel structure of the portal protein and the mispairing of subunits at the C-terminal affect the symmetry of portal rings. These findings expand the understanding of the structural characteristics of viral portal proteins.
JOURNAL OF MOLECULAR BIOLOGY
(2022)
Review
Plant Sciences
Tushar Ranjan, Awadhesh Kumar Pal, Bishun Deo Prasad, Ravi Ranjan Kumar, Mahesh Kumar, Md Shamim, Sanjay Jambhulkar
Summary: Plant viruses cause diseases in crops, leading to economic losses. Understanding their infection and spread is crucial for control strategies. Genome packaging is a key step in viral maturation, and recent studies show differences in packaging mechanisms and energy utilization in plant viruses compared to other systems.
AUSTRALASIAN PLANT PATHOLOGY
(2021)
Article
Virology
Venigalla B. Rao, Andrei Fokine, Qianglin Fang
Summary: The portal vertex is an important structure in many icosahedral viruses, playing a crucial role in genome packaging and infection. Recent research has revealed asymmetric interactions between the portal vertices, leading to a valve-like mechanism for regulating DNA flow.
CURRENT OPINION IN VIROLOGY
(2021)
Review
Genetics & Heredity
Tushar Ranjan, Ravi Ranjan Kumar, Mohammad Ansar, Jitesh Kumar, Auroshikha Mohanty, Anamika Kumari, Khushbu Jain, Kumari Rajani, Sailabala Dei, Mohammad Feza Ahmad
Summary: Genome packaging is a crucial step for plant viruses, and there are three reported packaging systems. The type I system involves energy-dependent nucleation and encapsidation of RNA genomes, while the type II and III systems involve translocation and packaging inside the prohead using ATP. Each system has a unique ATP hydrolysis and genome packaging mechanism.
FRONTIERS IN GENETICS
(2023)
Article
Virology
Emilio Reyes-Aldrete, Erik A. Dill, Cecile Bussetta, Michal R. Szymanski, Geoffrey Diemer, Priyank Maindola, Mark A. White, Wlodzimierz M. Bujalowski, Kyung H. Choi, Marc C. Morais
Summary: The study characterized the packaging ATPase from Lactococcus lactis phage ascc phi 28 using biochemical and biophysical methods. Results showed that gp11 forms a decameric D5-symmetric complex consisting of two pentameric rings related by 2-fold symmetry, and has ATPase activity comparable to functional ATPase rings on procapsids. The ability of gp11 to form functional rings in solution provides advantages for structural studies and rigorous analysis.
Article
Biochemistry & Molecular Biology
Hanjun Zhao, Chuyuan Zhang, Hoiyan Lam, Xinjie Meng, Zheng Peng, Man Lung Yeung, Jasper Fuk-Woo Chan, Kelvin Kai-Wang To, Kwok-Yung Yuen
Summary: It has been found that the specially designed SARS-CoV-2 and influenza defective interfering genes (DIGs) can significantly inhibit the replication of corresponding viruses. Through the gene delivery vector TAT2-P1&LAH4, DIGs can be effectively delivered in the lungs to inhibit the replication of SARS-CoV-2 variants and influenza virus.
SIGNAL TRANSDUCTION AND TARGETED THERAPY
(2022)
Article
Agriculture, Multidisciplinary
A. Mohanty, J. Kumar, T. Ranjan, R. R. Kumar, K. Rajani
Summary: Genome packaging is a critical step for viral maturation. Small plant viruses have evolved unique mechanisms to selectively package their genomes, involving not only capsid proteins but also other key players such as viral encoded movement proteins.
JOURNAL OF ANIMAL AND PLANT SCIENCES-JAPS
(2023)
Article
Genetics & Heredity
Ann M. Fallon
Summary: This study found that certain genes in Wolbachia phages and prophages have higher similarity to those in the gene transfer agent RcGTA than to bacteriophage lambda. These findings suggest that the structure and function of the WO phage genomes are more complex and extend beyond conventional phage lysogeny and lytic replication.