Efficient genome editing of an extreme thermophile, Thermus thermophilus, using a thermostable Cas9 variant

Recent Development of Extremophilic Bacteria and Their Application in Biorefinery

(2020) Daochen Zhu et al.   Frontiers in Bioengineering and Biotechnology

Genome editing of the anaerobic thermophile Thermoanaerobacter ethanolicus using thermostable Cas9

(2020) Yilin Le et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

CRISPR/Cas Systems towards Next-Generation Biosensing

(2019) Yi Li et al.   TRENDS IN BIOTECHNOLOGY

Selection-free markerless genome manipulations in the polyploid bacterium Thermus thermophilus

(2019) Haijuan Li   3 Biotech

Barriers to genome editing with CRISPR in bacteria

(2019) Justin M. Vento et al.   JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY

Nucleic acid cleavage with a hyperthermophilic Cas9 from an uncultured Ignavibacterium

(2019) Stephanie Tzouanas Schmidt et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Exploiting endogenous CRISPR-Cas system for multiplex genome editing in Clostridium tyrobutyricum and engineer the strain for high-level butanol production

(2018) Jie Zhang et al.   METABOLIC ENGINEERING

Chasing bacterial chassis for metabolic engineering: a perspective review from classical to non-traditional microorganisms

(2018) Patricia Calero et al.   Microbial Biotechnology

Harnessing the native type I-B CRISPR-Cas for genome editing in a polyploid archaeon

(2017) Feiyue Cheng et al.   Journal of Genetics and Genomics

Cre/lox-based multiple markerless gene disruption in the genome of the extreme thermophile Thermus thermophilus

(2017) Yoichiro Togawa et al.   MOLECULAR GENETICS AND GENOMICS

High tolerance to self-targeting of the genome by the endogenous CRISPR-Cas system in an archaeon

(2017) Aris-Edda Stachler et al.   NUCLEIC ACIDS RESEARCH

Characterizing a thermostable Cas9 for bacterial genome editing and silencing

(2017) Ioannis Mougiakos et al.   Nature Communications

A thermostable Cas9 with increased lifetime in human plasma

(2017) Lucas B. Harrington et al.   Nature Communications

The Impact of DNA Topology and Guide Length on Target Selection by a Cytosine-Specific Cas9

(2017) Tsz Kin Martin Tsui et al.   ACS Synthetic Biology

Efficient Genome Editing of a Facultative Thermophile Using Mesophilic spCas9

(2017) Ioannis Mougiakos et al.   ACS Synthetic Biology

Editing of the Bacillus subtilis Genome by the CRISPR-Cas9 System

(2016) Josef Altenbuchner   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

CRISPR technologies for bacterial systems: Current achievements and future directions

(2016) Kyeong Rok Choi et al.   BIOTECHNOLOGY ADVANCES

Ultrastructural Characterization of the Lower Motor System in a Mouse Model of Krabbe Disease

(2016) Valentina Cappello et al.   Scientific Reports

Markerless Gene Deletion with Cytosine Deaminase in Thermus thermophilus Strain HB27

(2015) Lei Wang et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Curing the Megaplasmid pTT27 from Thermus thermophilus HB27 and Maintaining Exogenous Plasmids in the Plasmid-Free Strain

(2015) Naoto Ohtani et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

A reporter gene system for the precise measurement of promoter activity in Thermus thermophilus HB27

(2015) Atsushi Fujita et al.   EXTREMOPHILES

Engineering the Genome of Thermus thermophilus Using a Counterselectable Marker

(2015) Jennifer F. Carr et al.   JOURNAL OF BACTERIOLOGY

Complete genome sequence, metabolic model construction and phenotypic characterization of Geobacillus LC300, an extremely thermophilic, fast growing, xylose-utilizing bacterium

(2015) Lauren T. Cordova et al.   METABOLIC ENGINEERING

Engineered CRISPR-Cas9 nucleases with altered PAM specificities

(2015) Benjamin P. Kleinstiver et al.   NATURE

An updated evolutionary classification of CRISPR–Cas systems

(2015) Kira S. Makarova et al.   NATURE REVIEWS MICROBIOLOGY

Harnessing Type I and Type III CRISPR-Cas systems for genome editing

(2015) Yingjun Li et al.   NUCLEIC ACIDS RESEARCH

Extremely thermophilic microorganisms as metabolic engineering platforms for production of fuels and industrial chemicals

(2015) Benjamin M. Zeldes et al.   Frontiers in Microbiology

RNA-guided editing of bacterial genomes using CRISPR-Cas systems

(2013) Wenyan Jiang et al.   NATURE BIOTECHNOLOGY

Toxicity of indoxyl derivative accumulation in bacteria and its use as a new counterselection principle

(2013) Angel Angelov et al.   SYSTEMATIC AND APPLIED MICROBIOLOGY

A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity

(2012) M. Jinek et al.   SCIENCE

CRISPR RNA maturation by trans-encoded small RNA and host factor RNase III

(2011) Elitza Deltcheva et al.   NATURE

Evolution and classification of the CRISPR–Cas systems

(2011) Kira S. Makarova et al.   NATURE REVIEWS MICROBIOLOGY

Simultaneous polyhydroxyalkanoates and rhamnolipids production by Thermus thermophilus HB8

(2011) Anastasia A Pantazaki et al.   AMB Express

An Extreme Thermophile, Thermus thermophilus, Is a Polyploid Bacterium

(2010) N. Ohtani et al.   JOURNAL OF BACTERIOLOGY

The CRISPR/Cas bacterial immune system cleaves bacteriophage and plasmid DNA

(2010) Josiane E. Garneau et al.   NATURE

Thermus thermophilus as biological model

(2009) Felipe Cava et al.   EXTREMOPHILES

Short motif sequences determine the targets of the prokaryotic CRISPR defence system

(2009)   MICROBIOLOGY-SGM

Small CRISPR RNAs Guide Antiviral Defense in Prokaryotes

(2008) S. J. J. Brouns et al.   SCIENCE