Plant genome editing: ever more precise and wide reaching

sgRNACNN: identifying sgRNA on-target activity in four crops using ensembles of convolutional neural networks

(2021) Mengting Niu et al.   PLANT MOLECULAR BIOLOGY

Advances in Genome Editing With CRISPR Systems and Transformation Technologies for Plant DNA Manipulation

(2021) Satya Swathi Nadakuduti et al.   Frontiers in Plant Science

CRISPR/Cas9 Induced Somatic Recombination at the CRTISO Locus in Tomato

(2021) Ilan Ben Shlush et al.   Genes

Genome engineering for crop improvement and future agriculture

(2021) Caixia Gao   CELL

A piggyBac ‐mediated transgenesis system for the temporary expression of CRISPR/Cas9 in rice

(2021) Ayako Nishizawa‐Yokoi et al.   PLANT BIOTECHNOLOGY JOURNAL

Targeted, random mutagenesis of plant genes with dual cytosine and adenine base editors

(2020) Chao Li et al.   NATURE BIOTECHNOLOGY

Targeted base editing in rice with CRISPR/ScCas9 system

(2020) Meixia Wang et al.   PLANT BIOTECHNOLOGY JOURNAL

Plant Prime Editors Enable Precise Gene Editing in Rice Cells

(2020) Xu Tang et al.   Molecular Plant

Precise Modifications of Both Exogenous and Endogenous Genes in Rice by Prime Editing

(2020) Huiyuan Li et al.   Molecular Plant

Versatile Nucleotides Substitution in Plant Using an Improved Prime Editing System

(2020) Wen Xu et al.   Molecular Plant

Superior field performance of waxy corn engineered using CRISPR–Cas9

(2020) Huirong Gao et al.   NATURE BIOTECHNOLOGY

Prime genome editing in rice and wheat

(2020) Qiupeng Lin et al.   NATURE BIOTECHNOLOGY

An engineered ScCas9 with broad PAM range and high specificity and activity

(2020) Pranam Chatterjee et al.   NATURE BIOTECHNOLOGY

Engineering herbicide resistance via prime editing in rice

(2020) Haroon Butt et al.   PLANT BIOTECHNOLOGY JOURNAL

Precision genome engineering in rice using prime editing system

(2020) Kai Hua et al.   PLANT BIOTECHNOLOGY JOURNAL

Site‐directed mutagenesis in bread and durum wheat via pollination by cas9 /guide RNA‐transgenic maize used as haploidy inducer

(2020) Nagaveni Budhagatapalli et al.   PLANT BIOTECHNOLOGY JOURNAL

Unconstrained genome targeting with near-PAMless engineered CRISPR-Cas9 variants

(2020) Russell T. Walton et al.   SCIENCE

Genome editing with CRISPR–Cas nucleases, base editors, transposases and prime editors

(2020) Andrew V. Anzalone et al.   NATURE BIOTECHNOLOGY

CRISPR C-to-G base editors for inducing targeted DNA transversions in human cells

(2020) Ibrahim C. Kurt et al.   NATURE BIOTECHNOLOGY

Glycosylase base editors enable C-to-A and C-to-G base changes

(2020) Dongdong Zhao et al.   NATURE BIOTECHNOLOGY

In planta particle bombardment (iPB): A new method for plant transformation and genome editing

(2020) Ryozo Imai et al.   Plant Biotechnology

Targeted genome editing in tetraploid potato through transient TALEN expression by Agrobacterium infection

(2020) Shuhei Yasumoto et al.   Plant Biotechnology

A GRF–GIF chimeric protein improves the regeneration efficiency of transgenic plants

(2020) Juan M. Debernardi et al.   NATURE BIOTECHNOLOGY

Applications of CRISPR–Cas in agriculture and plant biotechnology

(2020) Haocheng Zhu et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Potato virus X Vector-Mediated DNA-Free genome editing in plants

(2020) Hirotaka Ariga et al.   PLANT AND CELL PHYSIOLOGY

Precision genome editing in plants via gene targeting and subsequent break‐induced single‐strand annealing

(2020) Masaki Endo et al.   PLANT BIOTECHNOLOGY JOURNAL

Nanoparticle‐mediated gene transformation strategies for plant genetic engineering

(2020) Zongyou Lv et al.   PLANT JOURNAL

Changing local recombination patterns in Arabidopsis by CRISPR/Cas mediated chromosome engineering

(2020) Carla Schmidt et al.   Nature Communications

I-SceI Endonuclease-Mediated Plant Genome Editing by Protein Transport through a Bacterial Type III Secretion System

(2020) Yuki Yanagawa et al.   Plants-Basel

Efficient induction of heritable inversions in plant genomes using the CRISPR /Cas system

(2019) Carla Schmidt et al.   PLANT JOURNAL

Precise gene replacement in rice by RNA transcript-templated homologous recombination

(2019) Shaoya Li et al.   NATURE BIOTECHNOLOGY

One-step genome editing of elite crop germplasm during haploid induction

(2019) Timothy Kelliher et al.   NATURE BIOTECHNOLOGY

Improving plant genome editing with high-fidelity xCas9 and non-canonical PAM-targeting Cas9-NG

(2019) Zhaohui Zhong et al.   Molecular Plant

An adenine base editor with expanded targeting scope using SpCas9‐ NG v1 in rice

(2019) Katsuya Negishi et al.   PLANT BIOTECHNOLOGY JOURNAL

Centromere histone H3- and phospholipase-mediated haploid induction in plants

(2019) Song Wang et al.   Plant Methods

A barley stripe mosaic virus‐based guide RNA delivery system for targeted mutagenesis in wheat and maize

(2019) Jiacheng Hu et al.   MOLECULAR PLANT PATHOLOGY

Simplified adenine base editors improve adenine base editing efficiency in rice

(2019) Kai Hua et al.   PLANT BIOTECHNOLOGY JOURNAL

Multiplex nucleotide editing by high-fidelity Cas9 variants with improved efficiency in rice

(2019) Wen Xu et al.   BMC PLANT BIOLOGY

Search-and-replace genome editing without double-strand breaks or donor DNA

(2019) Andrew V. Anzalone et al.   NATURE

Engineering CRISPR/ Lb Cas12a for highly efficient, temperature‐tolerant plant gene editing

(2019) Patrick Schindele et al.   PLANT BIOTECHNOLOGY JOURNAL

Gene targeting in Arabidopsis via an all‐in‐one strategy that uses a translational enhancer to aid Cas9 expression

(2019) Fangnan Peng et al.   PLANT BIOTECHNOLOGY JOURNAL

Rapid customization of Solanaceae fruit crops for urban agriculture

(2019) Choon-Tak Kwon et al.   NATURE BIOTECHNOLOGY

Plant gene editing through de novo induction of meristems

(2019) Michael F. Maher et al.   NATURE BIOTECHNOLOGY

Improved Base Editor for Efficiently Inducing Genetic Variations in Rice with CRISPR/Cas9-Guided Hyperactive hAID Mutant

(2018) Bin Ren et al.   Molecular Plant

Base editing with a Cpf1–cytidine deaminase fusion

(2018) Xiaosa Li et al.   NATURE BIOTECHNOLOGY

Genotyping genome-edited mutations in plants using CRISPR ribonucleoprotein complexes

(2018) Zhen Liang et al.   PLANT BIOTECHNOLOGY JOURNAL

A method for the production and expedient screening of CRISPR/Cas9-mediated non-transgenic mutant plants

(2018) Longzheng Chen et al.   Horticulture Research

Evolved Cas9 variants with broad PAM compatibility and high DNA specificity

(2018) Johnny H. Hu et al.   NATURE

Genome editing of upstream open reading frames enables translational control in plants

(2018) Huawei Zhang et al.   NATURE BIOTECHNOLOGY

Expanding the base editing scope in rice by using Cas9 variants

(2018) Kai Hua et al.   PLANT BIOTECHNOLOGY JOURNAL

Engineered CRISPR-Cas9 nuclease with expanded targeting space

(2018) Hiroshi Nishimasu et al.   SCIENCE

Biolistic-delivery-based transient CRISPR/Cas9 expression enables in planta genome editing in wheat

(2018) Haruyasu Hamada et al.   Scientific Reports

Efficient C-to-T base editing in plants using a fusion of nCas9 and human APOBEC3A

(2018) Yuan Zong et al.   NATURE BIOTECHNOLOGY

Domestication of wild tomato is accelerated by genome editing

(2018) Tingdong Li et al.   NATURE BIOTECHNOLOGY

De novo domestication of wild tomato using genome editing

(2018) Agustin Zsögön et al.   NATURE BIOTECHNOLOGY

Developing a highly efficient and wildly adaptive CRISPR ‐ Sa Cas9 toolset for plant genome editing

(2018) Ruying Qin et al.   PLANT BIOTECHNOLOGY JOURNAL

Minimal PAM specificity of a highly similar SpCas9 ortholog

(2018) Pranam Chatterjee et al.   Science Advances

MATRILINEAL, a sperm-specific phospholipase, triggers maize haploid induction

(2017) Timothy Kelliher et al.   NATURE

Programmable base editing of A•T to G•C in genomic DNA without DNA cleavage

(2017) Nicole M. Gaudelli et al.   NATURE

Precise base editing in rice, wheat and maize with a Cas9-cytidine deaminase fusion

(2017) Yuan Zong et al.   NATURE BIOTECHNOLOGY

Targeted base editing in rice and tomato using a CRISPR-Cas9 cytidine deaminase fusion

(2017) Zenpei Shimatani et al.   NATURE BIOTECHNOLOGY

Programming sites of meiotic crossovers using Spo11 fusion proteins

(2017) Roberta Sarno et al.   NUCLEIC ACIDS RESEARCH

Targeted recombination between homologous chromosomes for precise breeding in tomato

(2017) Shdema Filler Hayut et al.   Nature Communications

Efficient DNA-free genome editing of bread wheat using CRISPR/Cas9 ribonucleoprotein complexes

(2017) Zhen Liang et al.   Nature Communications

An in planta biolistic method for stable wheat transformation

(2017) Haruyasu Hamada et al.   Scientific Reports

Efficient CRISPR/Cas9-Mediated Genome Editing Using a Chimeric Single-Guide RNA Molecule

(2017) Haroon Butt et al.   Frontiers in Plant Science

Programmable editing of a target base in genomic DNA without double-stranded DNA cleavage

(2016) Alexis C. Komor et al.   NATURE

Precision Targeted Mutagenesis via Cas9 Paired Nickases in Rice

(2016) Masafumi Mikami et al.   PLANT AND CELL PHYSIOLOGY

Morphogenic Regulators Baby boom and Wuschel Improve Monocot Transformation

(2016) Keith Lowe et al.   PLANT CELL

Targeted nucleotide editing using hybrid prokaryotic and vertebrate adaptive immune systems

(2016) K. Nishida et al.   SCIENCE

Highly specific targeted mutagenesis in plants using Staphylococcus aureus Cas9

(2016) Hidetaka Kaya et al.   Scientific Reports

Efficient targeted mutagenesis of rice and tobacco genomes using Cpf1 from Francisella novicida

(2016) Akira Endo et al.   Scientific Reports

Efficient Virus-Mediated Genome Editing in Plants Using the CRISPR/Cas9 System

(2015) Zahir Ali et al.   Molecular Plant

Highly efficient heritable plant genome engineering using Cas9 orthologues fromStreptococcus thermophilusandStaphylococcus aureus

(2015) Jeannette Steinert et al.   PLANT JOURNAL

Targeted Mutagenesis, Precise Gene Editing, and Site-Specific Gene Insertion in Maize Using Cas9 and Guide RNA

(2015) Sergei Svitashev et al.   PLANT PHYSIOLOGY

Positive–negative-selection-mediated gene targeting in rice

(2015) Zenpei Shimatani et al.   Frontiers in Plant Science

Egg cell-specific promoter-controlled CRISPR/Cas9 efficiently generates homozygous mutants for multiple target genes in Arabidopsis in a single generation

(2015) Zhi-Ping Wang et al.   GENOME BIOLOGY

Large chromosomal deletions and heritable small genetic changes induced by CRISPR/Cas9 in rice

(2014) Huanbin Zhou et al.   NUCLEIC ACIDS RESEARCH

Precision genome editing in plants via gene targeting andpiggyBac-mediated marker excision

(2014) Ayako Nishizawa-Yokoi et al.   PLANT JOURNAL

High-frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells

(2013) Yanfang Fu et al.   NATURE BIOTECHNOLOGY

Gene Editing a Constitutively Active OsRac1 by Homologous Recombination-Based Gene Targeting Induces Immune Responses in Rice

(2013) Thu Thi Dang et al.   PLANT AND CELL PHYSIOLOGY

Towards a Highly Efficient Gene Targeting System in Higher Plants

(2012) Hiroaki SAIKA et al.   JARQ-JAPAN AGRICULTURAL RESEARCH QUARTERLY

In planta gene targeting

(2012) F. Fauser et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity

(2012) M. Jinek et al.   SCIENCE

Cre-loxP mediated marker elimination and gene reactivation at the waxy locus created in rice genome based on strong positive-negative selection

(2011) Rie Terada et al.   Plant Biotechnology

Application of Gene Targeting to Designed Mutation Breeding of High-Tryptophan Rice

(2011) H. Saika et al.   PLANT PHYSIOLOGY

Haploid plants produced by centromere-mediated genome elimination

(2010) Maruthachalam Ravi et al.   NATURE