CRISPR-Cas systems for diagnosing infectious diseases

A CRISPR/Cas13a-powered catalytic electrochemical biosensor for successive and highly sensitive RNA diagnostics

(2021) Yan Sheng et al.   BIOSENSORS & BIOELECTRONICS

Development of a Broadly Applicable Cas12a-Linked Beam Unlocking Reaction for Sensitive and Specific Detection of Respiratory Pathogens Including SARS-CoV-2

(2021) Yu Wang et al.   ACS Chemical Biology

One-tube SARS-CoV-2 detection platform based on RT-RPA and CRISPR/Cas12a

(2021) Yangyang Sun et al.   Journal of Translational Medicine

CRISPR-ENHANCE: An enhanced nucleic acid detection platform using Cas12a

(2021) Long T. Nguyen et al.   METHODS

CRISPR Systems for COVID-19 Diagnosis

(2021) Hossein Rahimi et al.   ACS Sensors

Fluorescence polarization system for rapid COVID-19 diagnosis

(2021) Chang Yeol Lee et al.   BIOSENSORS & BIOELECTRONICS

Clustered Regularly Interspaced Short Palindromic Repeats/Cas9-Mediated Lateral Flow Nucleic Acid Assay

(2020) Xusheng Wang et al.   ACS Nano

Cross‐species transmission of the newly identified coronavirus 2019‐nCoV

(2020) Wei Ji et al.   JOURNAL OF MEDICAL VIROLOGY

New SARS-like virus in China triggers alarm

(2020) Jon Cohen et al.   SCIENCE

CRISPR/Cas12a-Mediated Interfacial Cleaving of Hairpin DNA Reporter for Electrochemical Nucleic Acid Sensing

(2020) Decai Zhang et al.   ACS Sensors

High-throughput and all-solution phase African Swine Fever Virus (ASFV) detection using CRISPR-Cas12a and fluorescence based point-of-care system

(2020) Qian He et al.   BIOSENSORS & BIOELECTRONICS

Ultra-sensitive and high-throughput CRISPR-Powered COVID-19 diagnosis

(2020) Zhen Huang et al.   BIOSENSORS & BIOELECTRONICS

Highly sensitive and specific detection of hepatitis B virus DNA and drug resistance mutations utilizing the PCR-based CRISPR-Cas13a system

(2020) S. Wang et al.   CLINICAL MICROBIOLOGY AND INFECTION

Real-time tracking of self-reported symptoms to predict potential COVID-19

(2020) Cristina Menni et al.   NATURE MEDICINE

Rapid and sensitive detection of COVID-19 using CRISPR/Cas12a-based detection with naked eye readout, CRISPR/Cas12a-NER

(2020) Xinjie Wang et al.   Science Bulletin

SARS-CoV-2 detection with CRISPR diagnostics

(2020) Lu Guo et al.   Cell Discovery

Clearing of Foreign Episomal DNA from Human Cells by CRISPRa-Mediated Activation of Cytidine Deaminases

(2020) Sergey Brezgin et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Detection of SARS-CoV-2 with SHERLOCK One-Pot Testing

(2020) Julia Joung et al.   NEW ENGLAND JOURNAL OF MEDICINE

Deployable CRISPR-Cas13a diagnostic tools to detect and report Ebola and Lassa virus cases in real-time

(2020) Kayla G. Barnes et al.   Nature Communications

opvCRISPR: One-pot visual RT-LAMP-CRISPR platform for SARS-cov-2 detection

(2020) Rui Wang et al.   BIOSENSORS & BIOELECTRONICS

Amplification-free detection of SARS-CoV-2 with CRISPR-Cas13a and mobile phone microscopy

(2020) Parinaz Fozouni et al.   CELL

Streamlined inactivation, amplification, and Cas13-based detection of SARS-CoV-2

(2020) Jon Arizti-Sanz et al.   Nature Communications

Rapid and Fully Microfluidic Ebola Virus Detection with CRISPR-Cas13a

(2019) Peiwu Qin et al.   ACS Sensors

CRISPR-Cas13a Nanomachine Based Simple Technology for Avian Influenza A (H7N9) Virus On-Site Detection

(2019) Yufeng Liu et al.   Journal of Biomedical Nanotechnology

Room Temperature Detection of Plasma Epstein–Barr Virus DNA with CRISPR–Cas13

(2019) Yetao Wu et al.   CLINICAL CHEMISTRY

Detection of unamplified target genes via CRISPR–Cas9 immobilized on a graphene field-effect transistor

(2019) Reza Hajian et al.   Nature Biomedical Engineering

CRISPR-Cas14 is now part of the artillery for gene editing and molecular diagnostic

(2019) Guillermo Aquino-Jarquin   Nanomedicine-Nanotechnology Biology and Medicine

Increasing the specificity of CRISPR systems with engineered RNA secondary structures

(2019) D. Dewran Kocak et al.   NATURE BIOTECHNOLOGY

CHOPCHOP v3: expanding the CRISPR web toolbox beyond genome editing

(2019) Kornel Labun et al.   NUCLEIC ACIDS RESEARCH

FLASH: a next-generation CRISPR diagnostic for multiplexed detection of antimicrobial resistance sequences

(2019) Jenai Quan et al.   NUCLEIC ACIDS RESEARCH

Origins and evolution of CRISPR-Cas systems

(2019) Eugene V. Koonin et al.   PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES

SHERLOCK: nucleic acid detection with CRISPR nucleases

(2019) Max J. Kellner et al.   Nature Protocols

HOLMESv2: A CRISPR-Cas12b-Assisted Platform for Nucleic Acid Detection and DNA Methylation Quantitation

(2019) Linxian Li et al.   ACS Synthetic Biology

Exploring the Trans-Cleavage Activity of CRISPR-Cas12a (cpf1) for the Development of a Universal Electrochemical Biosensor

(2019) Yifan Dai et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Dead Cas Systems: Types, Principles, and Applications

(2019) Sergey Brezgin et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

A CRISPR-Cas12a-derived biosensing platform for the highly sensitive detection of diverse small molecules

(2019) Mindong Liang et al.   Nature Communications

Programmable Inhibition and Detection of RNA Viruses Using Cas13

(2019) Catherine A. Freije et al.   MOLECULAR CELL

Detection of target DNA with a novel Cas9/sgRNAs-associated reverse PCR (CARP) technique

(2018) Beibei Zhang et al.   ANALYTICAL AND BIOANALYTICAL CHEMISTRY

Clustered Regularly Interspaced Short Palindromic Repeats/Cas9 Triggered Isothermal Amplification for Site-Specific Nucleic Acid Detection

(2018) Mengqi Huang et al.   ANALYTICAL CHEMISTRY

Biomarkers in Infectious Diseases

(2018) Hyundoo Hwang et al.   DISEASE MARKERS

A highly specific SpCas9 variant is identified by in vivo screening in yeast

(2018) Antonio Casini et al.   NATURE BIOTECHNOLOGY

Field-deployable viral diagnostics using CRISPR-Cas13

(2018) Cameron Myhrvold et al.   SCIENCE

CRISPR-Cas12a target binding unleashes indiscriminate single-stranded DNase activity

(2018) Janice S. Chen et al.   SCIENCE

Multiplexed and portable nucleic acid detection platform with Cas13, Cas12a, and Csm6

(2018) Jonathan S. Gootenberg et al.   SCIENCE

A Rapid and Specific Assay for the Detection of MERS-CoV

(2018) Pei Huang et al.   Frontiers in Microbiology

CRISPR-Cas12a-assisted nucleic acid detection

(2018) Shi-Yuan Li et al.   Cell Discovery

Detecting and typing target DNA with a novel CRISPR-typing PCR (ctPCR) technique

(2018) Beibei Zhang et al.   ANALYTICAL BIOCHEMISTRY

Directed evolution of CRISPR-Cas9 to increase its specificity

(2018) Jungjoon K. Lee et al.   Nature Communications

CRISPR-typing PCR (ctPCR), a new Cas9-based DNA detection method

(2018) Qiao Wang et al.   Scientific Reports

Novel coronavirus infections in Jordan, April 2012: epidemiological findings from a retrospective investigation

(2018) B. Hijawi et al.   EASTERN MEDITERRANEAN HEALTH JOURNAL

Functionally diverse type V CRISPR-Cas systems

(2018) Winston X. Yan et al.   SCIENCE

Programmed DNA destruction by miniature CRISPR-Cas14 enzymes

(2018) Lucas B. Harrington et al.   SCIENCE

A CRISPR–Cas9-triggered strand displacement amplification method for ultrasensitive DNA detection

(2018) Wenhua Zhou et al.   Nature Communications

Progress and Challenges towards Point-of-Care Diagnostic Development for Dengue

(2017) Junxiong Pang et al.   JOURNAL OF CLINICAL MICROBIOLOGY

Type III CRISPR–Cas systems produce cyclic oligoadenylate second messengers

(2017) Ole Niewoehner et al.   NATURE

RNA targeting with CRISPR–Cas13

(2017) Omar O. Abudayyeh et al.   NATURE

Diversity and evolution of class 2 CRISPR–Cas systems

(2017) Sergey Shmakov et al.   NATURE REVIEWS MICROBIOLOGY

RNA editing with CRISPR-Cas13

(2017) David B. T. Cox et al.   SCIENCE

A cyclic oligonucleotide signaling pathway in type III CRISPR-Cas systems

(2017) Migle Kazlauskiene et al.   SCIENCE

Nucleic acid detection with CRISPR-Cas13a/C2c2

(2017) Jonathan S. Gootenberg et al.   SCIENCE

Characterization of the interplay between DNA repair and CRISPR/Cas9-induced DNA lesions at an endogenous locus

(2017) Anne Bothmer et al.   Nature Communications

CRISPR/Cas9 in Genome Editing and Beyond

(2016) Haifeng Wang et al.   Annual Review of Biochemistry

Rapid, Low-Cost Detection of Zika Virus Using Programmable Biomolecular Components

(2016) Keith Pardee et al.   CELL

Zika Virus: Diagnostics for an Emerging Pandemic Threat

(2016) Jesse J. Waggoner et al.   JOURNAL OF CLINICAL MICROBIOLOGY

Zika virus: a previously slow pandemic spreads rapidly through the Americas

(2016) Derek Gatherer et al.   JOURNAL OF GENERAL VIROLOGY

Molecular Diagnostic Field Test for Point-of-Care Detection of Ebola Virus Directly From Blood

(2016) Jason W. Benzine et al.   JOURNAL OF INFECTIOUS DISEASES

Addressing barriers to the prevention, diagnosis and treatment of hepatitis B and C in the face of persisting fiscal constraints in Europe: report from a high level conference

(2016) G. Papatheodoridis et al.   JOURNAL OF VIRAL HEPATITIS

The global burden of dengue: an analysis from the Global Burden of Disease Study 2013

(2016) Jeffrey D Stanaway et al.   LANCET INFECTIOUS DISEASES

The Neisseria meningitidis CRISPR-Cas9 System Enables Specific Genome Editing in Mammalian Cells

(2016) Ciaran M Lee et al.   MOLECULAR THERAPY

Streptococcus thermophilus CRISPR-Cas9 Systems Enable Specific Editing of the Human Genome

(2016) Maximilian Müller et al.   MOLECULAR THERAPY

Structural basis for promiscuous PAM recognition in type I–E Cascade from E. coli

(2016) Robert P. Hayes et al.   NATURE

High-fidelity CRISPR–Cas9 nucleases with no detectable genome-wide off-target effects

(2016) Benjamin P. Kleinstiver et al.   NATURE

Genome-wide analysis reveals specificities of Cpf1 endonucleases in human cells

(2016) Daesik Kim et al.   NATURE BIOTECHNOLOGY

SARS and MERS: recent insights into emerging coronaviruses

(2016) Emmie de Wit et al.   NATURE REVIEWS MICROBIOLOGY

Diverse evolutionary roots and mechanistic variations of the CRISPR-Cas systems

(2016) Prarthana Mohanraju et al.   SCIENCE

Paired Design of dCas9 as a Systematic Platform for the Detection of Featured Nucleic Acid Sequences in Pathogenic Strains

(2016) Yihao Zhang et al.   ACS Synthetic Biology

Transmission characteristics of MERS and SARS in the healthcare setting: a comparative study

(2015) Gerardo Chowell et al.   BMC Medicine

IFN-α2a or IFN-β1a in combination with ribavirin to treat Middle East respiratory syndrome coronavirus pneumonia: a retrospective study

(2015) Sarah Shalhoub et al.   JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY

Will Ebola change the game? Ten essential reforms before the next pandemic. The report of the Harvard-LSHTM Independent Panel on the Global Response to Ebola

(2015) Suerie Moon et al.   LANCET

In vivo genome editing using Staphylococcus aureus Cas9

(2015) F. Ann Ran et al.   NATURE

A SARS-like cluster of circulating bat coronaviruses shows potential for human emergence

(2015) Vineet D Menachery et al.   NATURE MEDICINE

An updated evolutionary classification of CRISPR–Cas systems

(2015) Kira S. Makarova et al.   NATURE REVIEWS MICROBIOLOGY

Rationally engineered Cas9 nucleases with improved specificity

(2015) I. M. Slaymaker et al.   SCIENCE

Application of CRISPR/Cas9 for biomedical discoveries

(2015) Sean M. Riordan et al.   Cell and Bioscience

Enhanced Specificity and Efficiency of the CRISPR/Cas9 System with Optimized sgRNA Parameters in Drosophila

(2014) Xingjie Ren et al.   Cell Reports

TUBERCULOSIS: EPIDEMIOLOGY AND CONTROL

(2014) Giorgia Sulis et al.   Mediterranean Journal of Hematology and Infectious Diseases

Real-Time Reverse Transcription-PCR Assay Panel for Middle East Respiratory Syndrome Coronavirus

(2013) X. Lu et al.   JOURNAL OF CLINICAL MICROBIOLOGY

Isolation and characterization of a bat SARS-like coronavirus that uses the ACE2 receptor

(2013) Xing-Yi Ge et al.   NATURE

The global distribution and burden of dengue

(2013) Samir Bhatt et al.   NATURE

RNA-guided editing of bacterial genomes using CRISPR-Cas systems

(2013) Wenyan Jiang et al.   NATURE BIOTECHNOLOGY

Orthogonal Cas9 proteins for RNA-guided gene regulation and editing

(2013) Kevin M Esvelt et al.   NATURE METHODS

Multiplex Genome Engineering Using CRISPR/Cas Systems

(2013) L. Cong et al.   SCIENCE

A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity

(2012) M. Jinek et al.   SCIENCE

Patient with new strain of coronavirus is treated in intensive care at London hospital

(2012) J. Wise   BMJ-British Medical Journal

Introduction into PPPM as a new paradigm of public health service: an integrative view

(2012) Tatiana A Bodrova et al.   EPMA Journal

Sensitive Detection of microRNA with Isothermal Amplification and a Single-Quantum-Dot-Based Nanosensor

(2011) Yan Zhang et al.   ANALYTICAL CHEMISTRY

Awareness of infection, knowledge of hepatitis C, and medical follow-up among individuals testing positive for hepatitis C: National Health and Nutrition Examination Survey 2001-2008

(2011) Maxine M. Denniston et al.   HEPATOLOGY

Early Diagnosis of Dengue Infection Using a Commercial Dengue Duo Rapid Test Kit for the Detection of NS1, IGM, and IGG

(2010) Seok Mui Wang et al.   AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE

Detection of Mycobacterium tuberculosis resistance mutations to rifampin and isoniazid by real-time PCR

(2010) A Hristea et al.   Indian Journal of Medical Microbiology

Evaluation of the impact of single nucleotide polymorphisms and primer mismatches on quantitative PCR

(2009) Brian Boyle et al.   BMC BIOTECHNOLOGY

Small CRISPR RNAs Guide Antiviral Defense in Prokaryotes

(2008) S. J. J. Brouns et al.   SCIENCE