Review
Pharmacology & Pharmacy
Sihua Wu, Yufei Xia, Yuning Hu, Guanghui Ma
Summary: Bio-mimic particles are developed to mimic the natural traits of pathogens, providing optimal strategies to stimulate immune engagement and increase vaccine efficacy. Design principles include surface characteristics (hydrophobic, nano-pattern, antigen display, charge), properties (size, shape, softness), and delivered components (peptide, protein, nuclear acids, TLR agonist, antibody), along with efficient delivery strategies.
ADVANCED DRUG DELIVERY REVIEWS
(2021)
Article
Pharmacology & Pharmacy
Keegan Braz Gomes, Bernadette D'Souza, Sharon Vijayanand, Ipshita Menon, Martin J. D'Souza
Summary: Effective vaccines delivered via painless methods would revolutionize vaccination. This study developed fast-dissolving microneedles to deliver antigen-loaded polymeric nanoparticles, achieving a dual-delivery platform for transdermal vaccination. The study demonstrated the feasibility of this approach and its potential for transdermal vaccine administration.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2022)
Review
Chemistry, Multidisciplinary
Jie Tang, Larry Cai, Chuanfei Xu, Si Sun, Yuheng Liu, Joseph Rosenecker, Shan Guan
Summary: This review discusses recent developments in nucleic acid delivery systems targeting the airway mucosa for vaccination purposes, emphasizing the challenges and demands in delivering vaccines to the respiratory mucosa.
Article
Chemistry, Medicinal
Armira Azuar, Zhuoqing Li, Mohini A. Shibu, Lili Zhao, Yacheng Luo, Ahmed O. Shalash, Zeinab G. Khalil, Robert J. Capon, Waleed M. Hussein, Istvan Toth, Mariusz Skwarczynski
Summary: The study demonstrates the potential of self-adjuvanting poly(hydrophobic amino acids) as a delivery system for peptide-based vaccines against Group A Streptococcus (GAS). Conjugates with a linear arrangement of J8-PADRE and leucine were found to induce equally effective or even better immune responses compared to commercial adjuvants. The developed adjuvant-free vaccine based on single molecule successfully elicited antibodies capable of killing GAS bacteria.
JOURNAL OF MEDICINAL CHEMISTRY
(2021)
Article
Medicine, Research & Experimental
Asuka Takanashi, Colin W. Pouton, Hareth Al-Wassiti
Summary: Lipid nanoparticles (LNPs) are crucial for delivering mRNA vaccines. This study shows that LNPs not only induce immune responses in muscles, but also deliver mRNA to secondary lymphoid organs, leading to stronger adaptive immune responses.
MOLECULAR PHARMACEUTICS
(2023)
Article
Multidisciplinary Sciences
Sarah H. H. Park, Lin Lei, Darrel DSouza, Robert Zipkin, Emily T. T. DiMartini, Maria Atzampou, Emran O. O. Lallow, Jerry W. W. Shan, Jeffrey D. D. Zahn, David I. I. Shreiber, Hao Lin, Joel N. N. Maslow, Jonathan P. P. Singer
Summary: Electrospray deposition is a promising technique for depositing micro-/nano-scale droplets and particles with high quality and repeatability. This study shows that by architecting the local charge landscape, efficient coatings of surfaces smaller than the spray plume can be achieved. The process is proven to be an efficient alternative to other conformal coating mechanisms for micro-engineered applications.
NATURE COMMUNICATIONS
(2023)
Review
Chemistry, Inorganic & Nuclear
Manoj Kumar Sarangi, Sasmita Padhi, Goutam Rath, Sitansu Sekhar Nanda, Dong Kee Yi
Summary: The emergence of nanobiotechnology offers a unique opportunity for advancements in disease diagnosis and treatment. Gold nanoparticles (AuNPs) with functionalized surfaces have gained significant attention due to their unique properties, such as optical, electrical, magnetic, and catalytical properties. AuNPs have shown great potential in various biomedical applications including sensing, targeted drug delivery, bio-imaging, immunotherapy, vaccines, cancers, and infectious diseases, thanks to their key features such as electronic, optical, surface plasmon resonance, physicochemical, and immunomodulatory effects. Additionally, the low toxicity, biocompatibility, tunability, and stability of AuNPs make them promising candidates for diagnostic and therapeutic applications.
INORGANIC CHEMISTRY COMMUNICATIONS
(2023)
Article
Engineering, Biomedical
Shuxun Chen, Zhiwu Tan, Pan Liao, Yanfang Li, Yun Qu, Qi Zhang, Mingxuan Yang, Kannie Wai Yan Chan, Li Zhang, Kwan Man, Zhiwei Chen, Dong Sun
Summary: This study reports the fabrication of biocompatible and biodegradable GelMA microrobots through two-photon polymerization, and demonstrates programmed degradation and drug release by varying the local exposure dose. The GelMA microspheres are further functionalized for the delivery of DNA vaccines to dendritic cells and primary cells, leading to fast, enhanced, and durable antigen expression in mice.
ADVANCED HEALTHCARE MATERIALS
(2023)
Review
Chemistry, Physical
Adelina-Gabriela Niculescu, Alexandru Mihai Grumezescu
Summary: Polymer-based nanoparticles customized for size, morphology, and surface properties are gaining attention for drug, biomolecule, and gene delivery. They protect payloads from degradation and sustain controlled drug release, reducing clearance and increasing stability, solubility, and optimal concentration at target sites, especially in vaccine development.
Review
Virology
Eric A. Toth, Alexander K. K. Andrianov, Thomas R. R. Fuerst
Summary: Globally, there are more than 58 million people with chronic HCV infection, with 1.5 million new infections occurring annually. Developing an effective HCV vaccine is a major need, but generating broadly neutralising antibodies (bnAbs) has been difficult. Nanoparticle-based vaccines have gained attention due to their ability to facilitate multivalent presentation and interaction with the immune system. This review discusses the potential and challenges of developing a nanoparticle-based HCV E1E2 vaccine.
REVIEWS IN MEDICAL VIROLOGY
(2023)
Editorial Material
Chemistry, Medicinal
Lavinia M. Lewis, Advait Badkar, David Cirelli, Rodney Combs, Thomas F. Lerch
Summary: The Pfizer and BioNTech teams collaborated to develop a safe and effective mRNA COVID-19 vaccine in a record time, thanks to global collaboration and parallel efforts. Their success was based on foundational science, clinical trials, continuous improvement, and large-scale manufacturing and delivery.
JOURNAL OF PHARMACEUTICAL SCIENCES
(2023)
Article
Chemistry, Multidisciplinary
Masatoshi Maeki, Shuya Uno, Ayuka Niwa, Yuto Okada, Manabu Tokeshi
Summary: mRNA vaccines have been approved for COVID-19 prevention, thanks to the development of RNA-delivery technology such as lipid nanoparticles (LNPs). Recently, microfluidic devices and technologies have been utilized for the production of RNA-loaded LNPs, providing advantages such as precise control over LNP size, reproducibility, and high-throughput optimization.
JOURNAL OF CONTROLLED RELEASE
(2022)
Review
Engineering, Biomedical
William Ho, Mingzhu Gao, Fengqiao Li, Zhongyu Li, Xue-Qing Zhang, Xiaoyang Xu
Summary: Nucleic acid vaccines hold promise as an immunization method for combating pandemics, with nanoparticles being a top candidate for successful DNA/mRNA vaccine delivery due to their unique properties.
ADVANCED HEALTHCARE MATERIALS
(2021)
Review
Nanoscience & Nanotechnology
Kai Zhao, Yinzhuo Xie, Xuezheng Lin, Wei Xu
Summary: Mucosal immunization is an effective way to trigger mucosal immune responses, but most vaccines are currently administered through intramuscular injection, resulting in a lack of mucosal immune response. Biodegradable mucoadhesive nanoparticles show promise as vaccine delivery systems for mucosal administration.
INTERNATIONAL JOURNAL OF NANOMEDICINE
(2022)
Review
Pharmacology & Pharmacy
Jassica S. L. Leu, Jasy J. X. Teoh, Angel L. Q. Ling, Joey Chong, Yan Shan Loo, Intan Diana Mat Azmi, Noor Idayu Zahid, Rajendran J. C. Bose, Thiagarajan Madheswaran
Summary: Due to their unique structural features, lyotropic nonlamellar liquid crystalline nanoparticles (LCNPs) such as cubosomes and hexosomes are considered effective drug delivery systems. These nanostructures, often stabilized by surfactants, have a larger surface area than other lipid nanoparticles, allowing for the loading of therapeutic molecules, with the drug release being influenced by pore diameters. Recent research has focused on improving the preparation and characterization of LCNPs, as well as controlling drug release and enhancing the efficacy of bioactive chemicals. This article reviews the latest advancements in LCNP technology and provides an overview of their applications in biomedical fields based on different administration routes and their pharmacokinetic modulation properties.
Editorial Material
Pharmacology & Pharmacy
Aliasger K. Salem, Juliane Nguyen, Kristy M. Ainslie
Article
Biochemistry & Molecular Biology
Samuel Swearson, Aseel O. Rataan, Steven Eliason, Brad A. Amendt, Yousef Zakharia, Aliasger K. Salem, Thai Ho, Youcef M. Rustum
Summary: This study quantitated the expression levels of miR-17, -19a, -34a, -155, and -210 in different types of renal cell carcinoma cell lines and primary kidney tumors. The results showed that miR-17 and -19a were expressed at lower levels, while miR-34a, miR-155, and miR-210 were expressed at higher levels. In ccRCC cell lines with sarcomatoid differentiation, miR-34a was expressed higher than miR-210, -155, -17, and -19a. More samples are needed to confirm the statistical significance of the differences in miR expression levels in tumor biopsies. Previous studies have shown that miR-210, miR-155, and HIFs are druggable targets, and downregulation of these miRs may be a novel strategy for the treatment of advanced ccRCC.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Multidisciplinary Sciences
Rasheid Smith, Emad Wafa, Sean M. Geary, Kareem Ebeid, Suhaila O. Alhaj-Suliman, Aliasger K. Salem
Summary: Nanoscale formulations can enhance the efficacy of cancer vaccines by combating the immunosuppressive nature of the tumor microenvironment. A newly synthesized nanoparticulate formulation demonstrated adjuvant properties in vivo, leading to increased TAA-specific T cells, reduced tumor burden, protection against tumor rechallenge, and improved median survival in tumor-challenged mice.
Review
Pharmacology & Pharmacy
Supreeda Tambunlertchai, Sean M. Geary, Aliasger K. Salem
Summary: Melanoma is the deadliest form of skin cancer and surgery remains the preferred treatment. However, in some cases, alternative treatments are necessary. Resiquimod (RSQ) is an immunomodulatory molecule that shows promise as a potential treatment for melanoma.
Article
Engineering, Biomedical
Noah Z. Z. Laird, Pornpoj Phruttiwanichakun, Min Zhu, Jeffrey A. A. Banas, Satheesh Elangovan, Aliasger K. K. Salem
Summary: Choline and GEranic acid (CAGE) ionic liquids have been found to be useful in delivering macromolecules and poorly soluble drugs across epithelial barriers and inhibiting bacterial growth. They denature proteins by disrupting their folding forces and can inhibit elastase and other collagenolytic enzymes. This study investigated the inhibitory effects of CAGE with different stoichiometries on elastase and the growth of pathogenic oral bacteria. The results showed that CAGE could inhibit elastase and effectively suppress the growth of all tested bacteria. This inhibition of elastase may contribute to the improved outcomes in periodontitis treatment by CAGE, independent of its antibacterial properties.
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
(2023)
Article
Chemistry, Medicinal
Walla I. Malkawi, Noah Z. Laird, Pornpoj Phruttiwanichakun, Esraa Mohamed, Satheesh Elangovan, Aliasger K. Salem
Summary: Titanium is the preferred metal for dental implants due to its biocompatibility and ability to fuse with human bone tissue. Coating titanium surfaces with polyethyleneimine (PEI)-plasmid DNA (pDNA) polyplexes improved osseointegration by promoting therapeutic protein expression. The addition of sucralose as a lyoprotectant in the lyophilization process maintained the particle size and high transfection efficiency of the polyplexes. These findings suggest that modifying dental implants with lyophilized polyplexes can enhance their success rate in clinical settings.
JOURNAL OF PHARMACEUTICAL SCIENCES
(2023)
Article
Medicine, Research & Experimental
Ahmad Alkhazaleh, Sundes Elfagih, Leela Raghava Jaidev Chakka, Steven R. Armstrong, Carissa L. Comnick, Fang Qian, Aliasger K. Salem, C. Allan Guymon, Amanda J. Haes, Cristina M. P. Vidal
Summary: This study investigated the use of mesoporous silica nanoparticles (MSNs) for dental tissue biomodification. The incorporation of MSNs loaded with proanthocyanidin (PAC) improved the mechanical properties and bonding performance of dental restorative materials.
MOLECULAR PHARMACEUTICS
(2022)
Article
Medicine, Research & Experimental
Juliana C. Quarterman, Pornpoj Phruttiwanichakun, Douglas C. Fredericks, Aliasger K. Salem
Summary: Osteoarthritis patients may require revision surgery after joint replacement due to prosthetic loosening. Coating with zoledronic acid (ZA) has been shown to enhance implant osseointegration, as evidenced by in vitro and in vivo experiments.
MOLECULAR PHARMACEUTICS
(2022)
Article
Biotechnology & Applied Microbiology
M. M. Hasibuzzaman, Rui He, Ishrat Nourin Khan, Rasna Sabharwal, Aliasger K. K. Salem, Andrean Llewela Simons-Burnett
Summary: Delivery of IL-1 alpha using polymeric microparticles can suppress acute pro-inflammatory side effects while triggering an anti-tumor immune response.
BIOENGINEERING & TRANSLATIONAL MEDICINE
(2023)
Article
Pharmacology & Pharmacy
Supreeda Tambunlertchai, Sean M. Geary, Youssef W. Naguib, Aliasger K. Salem
Summary: Melanoma, the deadliest form of skin cancer, can be treated with surgery. However, there are cases where surgery fails or is not an option. Immunotherapy, specifically immune checkpoint blockade like anti-PD-1, can be an effective alternative treatment. In this study, the combination of anti-PD-1 and the TLR-7/8 agonist RSQ showed promising results in prolonging the survival of melanoma-challenged mice. RSQ also demonstrated direct anti-proliferative effects on melanoma cells in vitro.
Article
Engineering, Biomedical
Suhaila O. Alhaj-Suliman, Youssef W. Naguib, Emad I. Wafa, Sanjib Saha, Kareem Ebeid, Xiangbing Meng, Hamada H. Mohammed, Gamal El-Din A. Abuo-Rahma, Shujie Yang, Aliasger K. Salem
Summary: Dysfunction of p53 gene and presence of MDR1 gene are associated with many malignant tumors, including endometrial cancer, and contribute to drug resistance. A new ciprofloxacin derivative, CIP2b, in combination with taxanes, showed synergistic cytotoxic effects against endometrial cancer cells with MDR1 expression and loss-of-function p53. CIP2b targets multiple pathways and enhances the effects of paclitaxel on microtubule assembly.
Review
Clinical Neurology
Venkateswaran Ganesh, Yochana Kancherla, Cassim M. Igram, Andrew J. Pugely, Aliasger K. Salem, Kyungsup Shin, Tae-Hong Lim, Dongrim Seol
Summary: This study reviews pharmacotherapies and explores the feasibility of novel biologics and microRNA to reduce epidural fibrosis after laminectomy.
Review
Engineering, Biomedical
Alexandra McMillan, Nadia McMillan, Nikesh Gupta, Sohit P. Kanotra, Aliasger K. Salem
Summary: The development of tissue engineering and 3D bioprinting has opened up new possibilities for generating musculoskeletal tissue grafts in otolaryngology-head and neck surgery. However, the full potential of this technology has yet to be realized. Otolaryngology has the opportunity to lead in the clinical application of these engineered tissues.
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Chemistry, Multidisciplinary
Sudartip Areecheewakul, Andrea Adamcakova-Dodd, Zeb R. Zacharias, Xuefang Jing, David K. Meyerholz, Kevin L. Legge, Jon C. D. Houtman, Patrick T. O'Shaughnessy, Peter S. Thorne, Aliasger K. Salem
Summary: This study investigated the immunomodulatory effects of inhalation exposure to copper oxide nanoparticles (CuO NPs) in different immune conditions. The results showed that CuO NP exposure decreased T(H)1 and T(H)2 cells and increased T-bet(+) Treg cells in healthy mice. However, CuO NP inhalation exposure resulted in decreased T(H)2 responses and increased T-bet(+) Treg cells in mice with house dust mite (HDM) asthma, and increased T(H)2 cells in AIT-treated asthmatic mice.
Article
Chemistry, Multidisciplinary
Youssef W. Naguib, Suhaila O. Alhaj-Suliman, Emad I. Wafa, Sanjib Saha, Kareem Ebeid, Hamada H. H. Mohammed, Somaya A. Abdel-Rahman, Gamal El-Din A. Abuo-Rahma, Sean M. Geary, Aliasger K. Salem
Summary: Combining a novel ciprofloxacin derivative-loaded PEGylated polymeric nanoparticles (CIP2b-NPs) with paclitaxel (PTX) shows strong synergy in treating human type-II endometrial cancer. The tumor accumulation of CIP2b was significantly improved by formulating it into PEGylated polymeric nanoparticles, enhancing its overall antitumor activity when co-administered with PTX. The combination therapy of CIP2b-NPs and PTX demonstrates superior therapeutic activity in targeting human type-II endometrial cancer in a mouse model.
