Challenges in the application of NGS in the clinical laboratory

High-Throughput MICA/B Genotyping of Over Two Million Samples: Workflow and Allele Frequencies

(2020) Anja Klussmeier et al.   Frontiers in Immunology

Rapid high‐resolution HLA genotyping by MinION Oxford nanopore sequencing for deceased donor organ allocation

(2020) Dianne De Santis et al.   HLA

Accuracy of NGS HLA typing data influenced by STR

(2019) Hanneke W.M. van Deutekom et al.   HUMAN IMMUNOLOGY

Quality control project of NGS HLA genotyping for the 17th International HLA and Immunogenetics Workshop

(2019) Kazutoyo Osoegawa et al.   HUMAN IMMUNOLOGY

Next-generation sequencing reveals new information about HLA allele and haplotype diversity in a large European American population

(2019) Lisa E. Creary et al.   HUMAN IMMUNOLOGY

Accurate circular consensus long-read sequencing improves variant detection and assembly of a human genome

(2019) Aaron M. Wenger et al.   NATURE BIOTECHNOLOGY

A novel multiplexed 11 locus HLA full gene amplification assay using next generation sequencing

(2019) Linh Truong et al.   HLA

Evaluation of the AllType kit for HLA typing using the Ion Torrent S5 XL platform

(2019) Marine Cargou et al.   HLA

Nanopore sequencing and assembly of a human genome with ultra-long reads

(2018) Miten Jain et al.   NATURE BIOTECHNOLOGY

Next-generation sequencing technology a new tool for killer cell immunoglobulin-like receptor allele typing in hematopoietic stem cell transplantation

(2018) B. Maniangou et al.   TRANSFUSION CLINIQUE ET BIOLOGIQUE

The Importance of New Generation Sequencing (NGS) HLA Typing in Renal Transplantation—Preliminary Report

(2018) M. Kotowski et al.   TRANSPLANTATION PROCEEDINGS

Allelic Resolution NGS HLA Typing of Class I and Class II Loci and Haplotypes in Cape Town, South Africa

(2018) Yvonne R. Thorstenson et al.   HUMAN IMMUNOLOGY

HLA ‐ DR / DQ molecular mismatch: A prognostic biomarker for primary alloimmunity

(2018) Chris Wiebe et al.   AMERICAN JOURNAL OF TRANSPLANTATION

BulkVis: a graphical viewer for Oxford nanopore bulk FAST5 files

(2018) Alexander Payne et al.   BIOINFORMATICS

Bead-linked transposomes enable a normalization-free workflow for NGS library preparation

(2018) Stephen Bruinsma et al.   BMC GENOMICS

Design and Validation of a Multiplex KIR and HLA Class I Genotyping Method Using Next Generation Sequencing

(2018) Laia Closa et al.   Frontiers in Immunology

Reference Grade Characterization of Polymorphisms in Full-Length HLA Class I and II Genes With Short-Read Sequencing on the ION PGM System and Long-Reads Generated by Single Molecule, Real-Time Sequencing on the PacBio Platform

(2018) Shingo Suzuki et al.   Frontiers in Immunology

Sequences of 95 humanMHChaplotypes reveal extreme coding variation in genes other than highly polymorphicHLA class IandII

(2017) Paul J. Norman et al.   GENOME RESEARCH

Buccal swab genomic DNA fragmentation predicts likelihood of successful HLA genotyping by next-generation sequencing

(2017) Maureen C. Montgomery et al.   HUMAN IMMUNOLOGY

HLA genotyping using the Illumina HLA TruSight next-generation sequencing kits: A comparison

(2017) T. Profaizer et al.   International Journal of Immunogenetics

Killer Immunoglobulin-Like Receptor Allele Determination Using Next-Generation Sequencing Technology

(2017) Bercelin Maniangou et al.   Frontiers in Immunology

Dual redundant sequencing strategy: Full-length gene characterisation of 1056 novel and confirmatory HLA alleles

(2017) V. Albrecht et al.   HLA

High-throughput next-generation sequencing to genotype six classical HLA loci from 96 donors in a single MiSeq run

(2017) P. K. Ehrenberg et al.   HLA

Defining KIR and HLA Class I Genotypes at Highest Resolution via High-Throughput Sequencing

(2016) Paul J. Norman et al.   AMERICAN JOURNAL OF HUMAN GENETICS

Performance Characteristics and Validation of Next-Generation Sequencing for Human Leucocyte Antigen Typing

(2016) Eric T. Weimer et al.   JOURNAL OF MOLECULAR DIAGNOSTICS

Coverage recommendation for genotyping analysis of highly heterologous species using next-generation sequencing technology

(2016) Kai Song et al.   Scientific Reports

INC-Seq: accurate single molecule reads using nanopore sequencing

(2016) Chenhao Li et al.   GigaScience

Determining performance characteristics of an NGS-based HLA typing method for clinical applications

(2016) J. L. Duke et al.   HLA

Application of High-Throughput Next-Generation Sequencing for HLA Typing on Buccal Extracted DNA: Results from over 10,000 Donor Recruitment Samples

(2016) Yuxin Yin et al.   PLoS One

Cost-efficient multiplex PCR for routine genotyping of up to nine classical HLA loci in a single analytical run of multiple samples by next generation sequencing

(2015) Yuki Ozaki et al.   BMC GENOMICS

Report on the effects of fragment size, indexing, and read length on HLA sequencing on the Illumina MiSeq

(2015) T. Profaizer et al.   HUMAN IMMUNOLOGY

HLA-genotyping of clinical specimens using Ion Torrent-based NGS

(2015) Jonathan C. Barone et al.   HUMAN IMMUNOLOGY

Progressing towards the complete and thorough characterization of the HLA genes by NGS (or single-molecule DNA sequencing): Consequences, opportunities and challenges

(2015) Dimitri Monos et al.   HUMAN IMMUNOLOGY

Impact of three Illumina library construction methods on GC bias and HLA genotype calling

(2015) James H. Lan et al.   HUMAN IMMUNOLOGY

An integrated genotyping approach for HLA and other complex genetic systems

(2015) Wyatt C. Nelson et al.   HUMAN IMMUNOLOGY

The impact of next-generation sequencing technologies on HLA research

(2015) Kazuyoshi Hosomichi et al.   JOURNAL OF HUMAN GENETICS

High HLA-DP Expression and Graft-versus-Host Disease

(2015) Effie W. Petersdorf et al.   NEW ENGLAND JOURNAL OF MEDICINE

Development of a high-resolution NGS-based HLA-typing and analysis pipeline

(2015) M. Wittig et al.   NUCLEIC ACIDS RESEARCH

Tracking donor-reactive T cells: Evidence for clonal deletion in tolerant kidney transplant patients

(2015) Heather Morris et al.   Science Translational Medicine

HLA-A allele dropout in sequence-specific oligonucleotide probe typing due to intronic polymorphism in the novelA*31:01:02:02allele

(2015) A. Balas et al.   TISSUE ANTIGENS

PacBio Sequencing and Its Applications

(2015) Anthony Rhoads et al.   GENOMICS PROTEOMICS & BIOINFORMATICS

Biological significance of HLA locus matching in unrelated donor bone marrow transplantation

(2014) Y. Morishima et al.   BLOOD

Performance comparison of second- and third-generation sequencers using a bacterial genome with two chromosomes

(2014) Mari Miyamoto et al.   BMC GENOMICS

High-throughput multiplex HLA genotyping by next-generation sequencing using multi-locus individual tagging

(2014) Philip K Ehrenberg et al.   BMC GENOMICS

Cost-efficient high-throughput HLA typing by MiSeq amplicon sequencing

(2014) Vinzenz Lange et al.   BMC GENOMICS

Sequencing depth and coverage: key considerations in genomic analyses

(2014) David Sims et al.   NATURE REVIEWS GENETICS

Circulating Cell-Free DNA Enables Noninvasive Diagnosis of Heart Transplant Rejection

(2014) I. De Vlaminck et al.   Science Translational Medicine

Application of high-throughput, high-resolution and cost-effective next generation sequencing-based large-scale HLA typing in donor registry

(2014) M. Zhou et al.   TISSUE ANTIGENS

Phase-defined complete sequencing of the HLA genes by next-generation sequencing

(2013) Kazuyoshi Hosomichi et al.   BMC GENOMICS

Error rates for nanopore discrimination among cytosine, methylcytosine, and hydroxymethylcytosine along individual DNA strands

(2013) J. Schreiber et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Evaluation and optimisation of preparative semi-automated electrophoresis systems for Illumina library preparation

(2012) Michael A. Quail et al.   ELECTROPHORESIS

Comparison of Next-Generation Sequencing Systems

(2012) Lin Liu et al.   JOURNAL OF BIOMEDICINE AND BIOTECHNOLOGY

Hybrid error correction and de novo assembly of single-molecule sequencing reads

(2012) Sergey Koren et al.   NATURE BIOTECHNOLOGY

High-throughput, high-fidelity HLA genotyping with deep sequencing

(2012) C. Wang et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Targeted polymerase chain reaction-based enrichment and next generation sequencing for diagnostic testing of congenital disorders of glycosylation

(2011) Melanie A. Jones et al.   GENETICS IN MEDICINE

Next-generation sequencing: the solution for high-resolution, unambiguous human leukocyte antigen typing

(2010) C. Lind et al.   HUMAN IMMUNOLOGY

Cell-Free DNA as a Noninvasive Acute Rejection Marker in Renal Transplantation

(2009) V. Garcia Moreira et al.   CLINICAL CHEMISTRY