Automated production of CCR5-negative CD4+-T cells in a GMP-compatible, clinical scale for treatment of HIV-positive patients

Safe CRISPR/Cas9 inactivation of HIV-1 transcription with high specificity and broad-spectrum activity in latently infected cells by mutation of HIV-1 promoter NF-κB binding sites

(2020) Cheng-Han Chung et al.   Molecular Therapy-Nucleic Acids

Automated Manufacture of Autologous CD19 CAR-T Cells for Treatment of Non-hodgkin Lymphoma

(2020) Zachary Jackson et al.   Frontiers in Immunology

CAR T-cell therapy for cancer and HIV through novel approaches to HIV-associated haematological malignancies

(2020) Blake J Rust et al.   Lancet Haematology

Challenges and Opportunities of Using Adoptive T-Cell Therapy as Part of an HIV Cure Strategy

(2020) Yuqi Zhou et al.   JOURNAL OF INFECTIOUS DISEASES

HIV-1 remission following CCR5Δ32/Δ32 haematopoietic stem-cell transplantation

(2019) Ravindra K. Gupta et al.   NATURE

CRISPR-Edited Stem Cells in a Patient with HIV and Acute Lymphocytic Leukemia

(2019) Lei Xu et al.   NEW ENGLAND JOURNAL OF MEDICINE

GMP-Compliant Manufacturing of NKG2D CAR Memory T Cells Using CliniMACS Prodigy

(2019) Lucía Fernández et al.   Frontiers in Immunology

Closed-system manufacturing of CD19 and dual-targeted CD20/19 chimeric antigen receptor T cells using the CliniMACS Prodigy device at an academic medical center

(2018) Fenlu Zhu et al.   CYTOTHERAPY

Automated Manufacturing of Potent CD20-Directed Chimeric Antigen Receptor T Cells for Clinical Use

(2017) Dominik Lock et al.   HUMAN GENE THERAPY

GMP-production of purified human B lymphocytes for the adoptive transfer in patients after allogeneic hematopoietic stem cell transplantation

(2017) Hannes Tittlbach et al.   Journal of Translational Medicine

GMP-conformant on-site manufacturing of a CD133+ stem cell product for cardiovascular regeneration

(2017) Anna Skorska et al.   Stem Cell Research & Therapy

Engineering hematopoietic stem cells toward a functional cure of human immunodeficiency virus infection

(2016) Jianbin Wang et al.   CYTOTHERAPY

Automated manufacturing of chimeric antigen receptor T cells for adoptive immunotherapy using CliniMACS Prodigy

(2016) Ulrike Mock et al.   CYTOTHERAPY

Automated Enrichment, Transduction, and Expansion of Clinical-Scale CD62L+T Cells for Manufacturing of Gene Therapy Medicinal Products

(2016) Christoph Priesner et al.   HUMAN GENE THERAPY

Directed evolution of a recombinase that excises the provirus of most HIV-1 primary isolates with high specificity

(2016) Janet Karpinski et al.   NATURE BIOTECHNOLOGY

Digital PCR to assess gene-editing frequencies (GEF-dPCR) mediated by designer nucleases

(2016) Ulrike Mock et al.   Nature Protocols

Engineering T Cells to Functionally Cure HIV-1 Infection

(2015) Rachel S Leibman et al.   MOLECULAR THERAPY

mRNA transfection of a novel TAL effector nuclease (TALEN) facilitates efficient knockout of HIV co-receptor CCR5

(2015) U. Mock et al.   NUCLEIC ACIDS RESEARCH

TALEN-mediated editing of endogenous T-cell receptors facilitates efficient reprogramming of T lymphocytes by lentiviral gene transfer

(2014) B Berdien et al.   GENE THERAPY

CD4 Ligation on Human Blood Monocytes Triggers Macrophage Differentiation and Enhances HIV Infection

(2014) A. Zhen et al.   JOURNAL OF VIROLOGY

Gene Editing of CCR5 in Autologous CD4 T Cells of Persons Infected with HIV

(2014) Pablo Tebas et al.   NEW ENGLAND JOURNAL OF MEDICINE

An online bioinformatics tool predicts zinc finger and TALE nuclease off-target cleavage

(2014) Eli J. Fine et al.   NUCLEIC ACIDS RESEARCH

Multiplexing clonality: combining RGB marking and genetic barcoding

(2014) Kerstin Cornils et al.   NUCLEIC ACIDS RESEARCH

High-Throughput Genome Editing and Phenotyping Facilitated by High Resolution Melting Curve Analysis

(2014) Holly R. Thomas et al.   PLoS One

Novel lentiviral vectors with mutated reverse transcriptase for mRNA delivery of TALE nucleases

(2014) Ulrike Mock et al.   Scientific Reports

TAL Effector-Nucleotide Targeter (TALE-NT) 2.0: tools for TAL effector design and target prediction

(2012) Erin L. Doyle et al.   NUCLEIC ACIDS RESEARCH

Efficient Lentiviral Transduction and Transgene Expression in Primary Human B Cells

(2012) Ulrike Mock et al.   Human Gene Therapy Methods

Lymphoid Tissue Damage in HIV-1 Infection Depletes Naïve T Cells and Limits T Cell Reconstitution after Antiretroviral Therapy

(2012) Ming Zeng et al.   PLoS Pathogens

A novel TALE nuclease scaffold enables high genome editing activity in combination with low toxicity

(2011) Claudio Mussolino et al.   NUCLEIC ACIDS RESEARCH

Long-Term Control of HIV byCCR5Delta32/Delta32 Stem-Cell Transplantation

(2009) Gero Hütter et al.   NEW ENGLAND JOURNAL OF MEDICINE

Resistance of mature T cells to oncogene transformation

(2008) S. Newrzela et al.   BLOOD

A Multicolor Panel of Novel Lentiviral “Gene Ontology” (LeGO) Vectors for Functional Gene Analysis

(2008) Kristoffer Weber et al.   MOLECULAR THERAPY

Establishment of HIV-1 resistance in CD4+ T cells by genome editing using zinc-finger nucleases

(2008) Elena E Perez et al.   NATURE BIOTECHNOLOGY