Article
Biochemical Research Methods
Jorvani Cruz Villarreal, Rory Kruithoff, Ana Egatz-Gomez, Paul D. Coleman, Robert Ros, Todd R. Sandrin, Alexandra Ros
Summary: Understanding cell-to-cell variation at the molecular level is critical for research and this study presents a microfluidic platform combined with MALDI-TOF-MS for the detection and quantification of targeted proteins from small cell ensembles. The integrated on-chip approach allows for protein quantification from cell lysate and demonstrates promising potential for single-cell analysis.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Biochemical Research Methods
Yan Zhang, Sungho Kim, Weihua Shi, Yaoyao Zhao, Insu Park, Christopher Brenden, Hrishikesh Iyer, Prasoon Jha, Rashid Bashir, Jonathan V. Sweedler, Yurii Vlasov
Summary: The silicon microfluidic platform allows for the monolithic integration of transparent micron-scale microfluidic channels, on-chip segmentation of analyte flows into picoliter droplets, and spatial and temporal separation of oil and aqueous phases during electro-spray for subsequent mass spectrometry analysis.
Article
Chemistry, Analytical
Jing Chen, Fei Liu, Zhoumin Li, Li Tan, Mei Zhang, Danke Xu
Summary: A rapid and automated system for determination of aflatoxins in peanut oil was developed, integrating sample pretreatment into a microfluidic chip for the first time. Online mass spectrometry detection achieved with good linear calibration curves and low detection limits, demonstrating potential for application in various food samples.
MICROCHEMICAL JOURNAL
(2021)
Article
Chemistry, Analytical
E. Mavrakis, Z. Toprakcioglu, N. Lydakis-Simantiris, T. P. J. Knowles, S. A. Pergantis
Summary: As the use of microfluidic chips for biological samples handling increases, there is a growing demand to combine them with powerful analytical techniques such as ICP-MS. This study demonstrates the successful coupling of a chip-based supersonic microfluidic nebulizer to an ICP-MS through the use of a spray chamber with a laminar flow makeup gas. The system shows promising performance in single-particle and single-cell analysis.
ANALYTICA CHIMICA ACTA
(2022)
Review
Biochemical Research Methods
Long Ngo, Le Que Anh Pham, Anastasiia Tukova, Amin Hassanzadeh-Barforoushi, Wei Zhang, Yuling Wang
Summary: Cancer-derived small extracellular vesicles (sEVs) are specific subgroups of lipid bilayer vesicles secreted from cancer cells to the extracellular environment. They carry distinct biomolecules (e.g., proteins, lipids and nucleic acids) from their parent cancer cells. Therefore, the analysis of cancer-derived sEVs can provide valuable information for cancer diagnosis. However, the use of cancer-derived sEVs in clinics is still limited due to their small size, low amounts in circulating fluids, and heterogeneous molecular features, making their isolation and analysis challenging.
Article
Chemistry, Analytical
Yan Man, Meijing Ban, Xinxin Jin, An Li, Jing Tao, Ligang Pan
Summary: A portable and equipment-free microfluidic aptasensor was developed for on-site quantitative detection of Salmonella. This aptasensor utilized microcolumns-based vortex micromixing and incubation, nitrocellulose membranes with single-strand binding proteins (SSBP-NCMs) for enrichment and separation, and distance-readout for visual quantitative detection. The aptasensor showed good specificity and reliability, and could detect Salmonella typhimurium as low as 3.7 x 101 cfu mL-1.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Review
Chemistry, Analytical
Shuang Ding, Haijun Zhang, Xuemei Wang
Summary: Lung diseases pose serious threats to human health worldwide, and traditional models are unable to accurately simulate lung conditions. Human organ-on-a-chip platforms and biosensors can better mimic lung characteristics, providing new methods for studying disease development and drug efficacy.
Article
Biochemical Research Methods
Fangfang Fan, Ning Xu, Yucheng Sun, Xuanhao Li, Xinchang Gao, Xizhen Yi, Yi Zhang, Xianli Meng, Jin-Ming Lin
Summary: This study elucidated a new mechanism of Salidroside in reducing hypoxia-induced inflammation in microglia by regulating cellular energy metabolism. The hypoxic microenvironment of BV2 microglial cells was simulated in vitro using a cell microfluidic chip-mass spectrometry (CM-MS) system, and the real-time change of cell metabolites was investigated. The levels of hypoxia-related factors were also detected to confirm the metabolic mechanism of BV2 cells under hypoxia. The study found that Salidroside could invert the changes in cellular energy metabolism and identified two metabolites of Salidroside.
JOURNAL OF PROTEOME RESEARCH
(2022)
Article
Chemistry, Medicinal
Andreas Traberg, Fernanda E. Pinto, Anders C. N. Hansen, Merete Haedersdal, Catharina M. Lerche, Christian Janfelt
Summary: The aim of Quantitative mass spectrometry imaging (Q-MSI) is to provide distribution analysis and quantitation in one experiment. Mimetic tissue models based on spiked tissue homogenates are accurate methods for Q-MSI. The authors extended this model for skin studies, overcoming the high viscosity issue by adding water and handling the homogenate at an elevated temperature. They developed a method for quantitative imaging of bleomycin in skin using this model and included an internal standard to compensate for signal drift and skin inhomogeneities. The results of the model were comparable to a quantitative LC-MS analysis and it can replace or provide additional quantitative information to skin studies currently conducted by MSI alone.
Article
Multidisciplinary Sciences
Arnaud Cannet, Camille Simon-Chane, Mohammad Akhoundi, Aymeric Histace, Olivier Romain, Marc Souchaud, Pierre Jacob, Pascal Delaunay, Darian Sereno, Philippe Bousses, Pascal Grebaut, Anne Geiger, Chantel de Beer, Dramane Kaba, Denis Sereno
Summary: This study presents a new method for identifying Glossina species using Wing Interference Patterns (WIPs). By combining deep learning algorithms with a database of WIPs representing 23 Glossina species, accurate automatic recognition is achieved.
SCIENTIFIC REPORTS
(2022)
Article
Biochemical Research Methods
Huizhi Li, Ri Wu, Qiongzheng Hu, Xiangfeng Chen, T-W Dominic Chan
Summary: A new integrated matrix deposition device with synchronous solvent nebulization was developed for MALDI-mass spectrometry imaging, improving efficiency and quality in imaging of protein and small metabolite distributions in mouse brain tissue sections. The device demonstrated enhanced sensitivity and image quality compared to conventional methods, showing its utility in MALDI-MSI applications.
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2022)
Article
Chemistry, Analytical
Valentina V. Ilyushenkova, Marina E. Zimens, Nikolay Yu Polovkov, Artyom P. Topolyan, Roman S. Borisov, Vladimir G. Zaikin
Summary: This study presents an efficient method for the highly sensitive determination of dipeptides in biological liquids. The method involves derivatization of peptides followed by mass spectrometry, which allows for the detection of low quantities of dipeptides. The approach was successfully applied to model dipeptides in artificial urine and blood serum, and several dipeptides were identified in real blood serum samples. The use of collision induced dissociation generated characteristic fragmentation mass spectra for rapid identification and quantification. The derivatized dipeptides showed higher sensitivity compared to non-modified dipeptides. Overall, this derivatization approach is valuable for the identification of specific dipeptides in artificial media and dietary supplements.
Review
Chemistry, Analytical
Roman S. Borisov, Mariya D. Matveeva, Vladimir G. Zaikin
Summary: In recent years, there has been a special focus on the use of reactive matrices in MALDI-MS, which have dual properties and have been applied in bioanalysis to reduce sample treatment time and improve sensitivity. True reactive matrices interact with molecules through exchange or addition reactions, enhancing molecular identification and profiling.
CRITICAL REVIEWS IN ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Multidisciplinary
Zhaochen Su, Wanting Hu, Lizhen Ye, Dan Gao, Jin-Ming Lin
Summary: To restrain drug resistance development and reduce antibiotics abuse, it is important to rapidly identify bacteria producing fi-lactamases and propose a reasonable treatment plan. This study presents an integrated microfluidic chip-mass spectrometry system for rapid screening of fi-lactamase-producing bacteria and optimization of fi-lactamase inhibitor dosing concentration. The system allows analysis of the hydrolysis of antibiotics by fi-lactamase-producing bacteria and provides an easy and rapid protocol for fi-lactamase resistance studies.
CHINESE CHEMICAL LETTERS
(2023)
Article
Chemistry, Analytical
Shiyu Chen, Fangfang Fan, Yingrui Zhang, Jiuseng Zeng, Yuxuan Li, Ning Xu, Yi Zhang, Xian-Li Meng, Jin-Ming Lin
Summary: This study used a microfluidic-chip mass spectrometry method to investigate the drug metabolism changes of Scutellarin under hypoxia conditions. The system allowed real-time monitoring of metabolite changes and drug metabolism in BV2 cells. The findings shed light on the mechanism of Scutellarin in reducing hypoxia-induced injury and presented a novel strategy for monitoring the energy metabolic mechanism of drugs on cells.
Article
Chemistry, Analytical
Daihyun Kim, Austin Echelmeier, Jorvani Cruz Villarreal, Sahir Gandhi, Sebastian Quintana, Ana Egatz-Gomez, Alexandra Ros
ANALYTICAL CHEMISTRY
(2019)
Article
Chemistry, Multidisciplinary
Austin Echelmeier, Daihyun Kim, Jorvani Cruz Villarreal, Jesse Coe, Sebastian Quintana, Gerrit Brehm, Ana Egatz-Gomez, Reza Nazari, Raymond G. Sierra, Jason E. Koglin, Alexander Batyuk, Mark S. Hunter, Sebastien Boutet, Nadia Zatsepin, Richard A. Kirian, Thomas D. Grant, Petra Fromme, Alexandra Ros
JOURNAL OF APPLIED CRYSTALLOGRAPHY
(2019)
Article
Biochemical Research Methods
Suraj Pandey, Richard Bean, Tokushi Sato, Ishwor Poudyal, Johan Bielecki, Jorvani Cruz Villarreal, Oleksandr Yefanov, Valerio Mariani, Thomas A. White, Christopher Kupitz, Mark Hunter, Mohamed H. Abdellatif, Sasa Bajt, Valerii Bondar, Austin Echelmeier, Diandra Doppler, Moritz Emons, Matthias Frank, Raimund Fromme, Yaroslav Gevorkov, Gabriele Giovanetti, Man Jiang, Daihyun Kim, Yoonhee Kim, Henry Kirkwood, Anna Klimovskaia, Juraj Knoska, Faisal H. M. Koua, Romain Letrun, Stella Lisova, Luis Maia, Victoria Mazalova, Domingo Meza, Thomas Michelat, Abbas Ourmazd, Guido Palmer, Marco Ramilli, Robin Schubert, Peter Schwander, Alessandro Silenzi, Jolanta Sztuk-Dambietz, Alexandra Tolstikova, Henry N. Chapman, Alexandra Ros, Anton Barty, Petra Fromme, Adrian P. Mancuso, Marius Schmidt
Article
Multidisciplinary Sciences
Chris Gisriel, Jesse Coe, Romain Letrun, Oleksandr M. Yefanov, Cesar Luna-Chavez, Natasha E. Stander, Stella Lisova, Valerio Mariani, Manuela Kuhn, Steve Aplin, Thomas D. Grant, Katerina Doerner, Tokushi Sato, Austin Echelmeier, Jorvani Cruz Villarreal, Mark S. Hunter, Max O. Wiedorn, Juraj Knoska, Victoria Mazalova, Shatabdi Roy-Chowdhury, Jay-How Yang, Alex Jones, Richard Bean, Johan Bielecki, Yoonhee Kim, Grant Mills, Britta Weinhausen, Jose D. Meza, Nasser Al-Qudami, Sasa Bajt, Gerrit Brehm, Sabine Botha, Djelloul Boukhelef, Sandor Brockhauser, Barry D. Bruce, Matthew A. Coleman, Cyril Danilevski, Erin Discianno, Zachary Dobson, Hans Fangohr, Jose M. Martin-Garcia, Yaroslav Gevorkov, Steffen Hauf, Ahmad Hosseinizadeh, Friederike Januschek, Gihan K. Ketawala, Christopher Kupitz, Luis Maia, Maurizio Manetti, Marc Messerschmidt, Thomas Michelat, Jyotirmoy Mondal, Abbas Ourmazd, Gianpietro Previtali, Iosifina Sarrou, Silvan Schoen, Peter Schwander, Megan L. Shelby, Alessandro Silenzi, Jolanta Sztuk-Dambietz, Janusz Szuba, Monica Turcato, Thomas A. White, Krzysztof Wrona, Chen Xu, Mohamed H. Abdellatif, James D. Zook, John C. H. Spence, Henry N. Chapman, Anton Barty, Richard A. Kirian, Matthias Frank, Alexandra Ros, Marius Schmidt, Raimund Fromme, Adrian P. Mancuso, Petra Fromme, Nadia A. Zatsepin
NATURE COMMUNICATIONS
(2019)
Article
Multidisciplinary Sciences
Austin Echelmeier, Jorvani Cruz Villarreal, Marc Messerschmidt, Daihyun Kim, Jesse D. Coe, Darren Thifault, Sabine Botha, Ana Egatz-Gomez, Sahir Gandhi, Gerrit Brehm, Chelsie E. Conrad, Debra T. Hansen, Caleb Madsen, Sasa Bajt, J. Domingo Meza-Aguilar, Dominik Oberthur, Max O. Wiedorn, Holger Fleckenstein, Derek Mendez, Juraj Knoska, Jose M. Martin-Garcia, Hao Hu, Stella Lisova, Aschkan Allahgholi, Yaroslav Gevorkov, Kartik Ayyer, Steve Aplin, Helen Mary Ginn, Heinz Graafsma, Andrew J. Morgan, Dominic Greiffenberg, Alexander Klujev, Torsten Laurus, Jennifer Poehlsen, Ulrich Trunk, Davide Mezza, Bernd Schmidt, Manuela Kuhn, Raimund Fromme, Jolanta Sztuk-Dambietz, Natascha Raab, Steffen Hauf, Alessandro Silenzi, Thomas Michelat, Chen Xu, Cyril Danilevski, Andrea Parenti, Leonce Mekinda, Britta Weinhausen, Grant Mills, Patrik Vagovic, Yoonhee Kim, Henry Kirkwood, Richard Bean, Johan Bielecki, Stephan Stern, Klaus Giewekemeyer, Adam R. Round, Joachim Schulz, Katerina Doerner, Thomas D. Grant, Valerio Mariani, Anton Barty, Adrian P. Mancuso, Uwe Weierstall, John C. H. Spence, Henry N. Chapman, Nadia Zatsepin, Petra Fromme, Richard A. Kirian, Alexandra Ros
NATURE COMMUNICATIONS
(2020)
Article
Chemistry, Multidisciplinary
Diandra Doppler, Mohammad T. Rabbani, Romain Letrun, Jorvani Cruz Villarreal, Dai Hyun Kim, Sahir Gandhi, Ana Egatz-Gomez, Mukul Sonker, Joe Chen, Faisal H. M. Koua, Jayhow Yang, Mohamed Youssef, Victoria Mazalova, Sasa Bajt, Megan L. Shelby, Matt A. Coleman, Max O. Wiedorn, Juraj Knoska, Silvan Schon, Tokushi Sato, Mark S. Hunter, Ahmad Hosseinizadeh, Christopher Kuptiz, Reza Nazari, Roberto C. Alvarez, Konstantinos Karpos, Sahba Zaare, Zachary Dobson, Erin Discianno, Shangji Zhang, James D. Zook, Johan Bielecki, Raphael de Wijn, Adam R. Round, Patrik Vagovic, Marco Kloos, Mohammad Vakili, Gihan K. Ketawala, Natasha E. Stander, Tien L. Olson, Katherine Morin, Jyotirmory Mondal, Jonathan Nguyen, Jose Domingo Meza-Aguilar, Gerdenis Kodis, Sara Vaiana, Jose M. Martin-Garcia, Valerio Mariani, Peter Schwander, Marius Schmidt, Marc Messerschmidt, Abbas Ourmazd, Nadia Zatsepin, Uwe Weierstall, Barry D. Bruce, Adrian P. Mancuso, Thomas Grant, Anton Barty, Henry N. Chapman, Matthias Frank, Raimund Fromme, John C. H. Spence, Sabine Botha, Petra Fromme, Richard A. Kirian, Alexandra Ros
Summary: This study proposes a new method to address the issue of crystal introduction in the X-ray beam. By using an inert oil phase to supplement the flow of the sample, stable injection of crystals from two different materials can be achieved, avoiding crystal clogging and potentially increasing the preservation rate of crystal samples, enabling degradation-free light-induced time-resolved SFX.
JOURNAL OF APPLIED CRYSTALLOGRAPHY
(2022)
Article
Biochemical Research Methods
Jorvani Cruz Villarreal, Rory Kruithoff, Ana Egatz-Gomez, Paul D. Coleman, Robert Ros, Todd R. Sandrin, Alexandra Ros
Summary: Understanding cell-to-cell variation at the molecular level is critical for research and this study presents a microfluidic platform combined with MALDI-TOF-MS for the detection and quantification of targeted proteins from small cell ensembles. The integrated on-chip approach allows for protein quantification from cell lysate and demonstrates promising potential for single-cell analysis.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)