Affinity Capture of p97 with Small-Molecule Ligand Bait Reveals a 3.6 Å Double-Hexamer Cryoelectron Microscopy Structure

Entropic Inhibition: How the Activity of a AAA+ Machine Is Modulated by Its Substrate-Binding Domain

(2021) Marija Iljina et al.   ACS Chemical Biology

Estimation of high-order aberrations and anisotropic magnification from cryo-EM data sets in RELION-3.1

(2020) Jasenko Zivanov et al.   IUCrJ

Smart Molecular Nanosheets for Advanced Preparation of Biological Samples in Electron Cryo-Microscopy

(2020) Julian Scherr et al.   ACS Nano

General and robust covalently linked graphene oxide affinity grids for high-resolution cryo-EM

(2020) Feng Wang et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Bioactive Functionalized Monolayer Graphene for High-Resolution Cryo-Electron Microscopy

(2019) Nan Liu et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Multifunctional graphene supports for electron cryomicroscopy

(2019) Katerina Naydenova et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Structure of the Cdc48 segregase in the act of unfolding an authentic substrate

(2019) Ian Cooney et al.   SCIENCE

Substrate processing by the Cdc48 ATPase complex is initiated by ubiquitin unfolding

(2019) Edward C. Twomey et al.   SCIENCE

p97: An Emerging Target for Cancer, Neurodegenerative Diseases, and Viral Infections

(2019) Donna M. Huryn et al.   JOURNAL OF MEDICINAL CHEMISTRY

A simple and robust procedure for preparing graphene-oxide cryo-EM grids

(2018) Eugene Palovcak et al.   JOURNAL OF STRUCTURAL BIOLOGY

VCP/p97-Mediated Unfolding as a Principle in Protein Homeostasis and Signaling

(2018) Johannes van den Boom et al.   MOLECULAR CELL

Structure of the Cdc48 ATPase with its ubiquitin-binding cofactor Ufd1–Npl4

(2018) Nicholas O. Bodnar et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Structure of the Cdc48 ATPase with its ubiquitin-binding cofactor Ufd1–Npl4

(2018) Nicholas O. Bodnar et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Automated map sharpening by maximization of detail and connectivity

(2018) Thomas C. Terwilliger et al.   Acta Crystallographica Section D-Structural Biology

Optimization of Phenyl Indole Inhibitors of the AAA ATPase p97

(2018) Matthew G. LaPorte et al.   ACS Medicinal Chemistry Letters

New tools for automated high-resolution cryo-EM structure determination in RELION-3

(2018) Jasenko Zivanov et al.   eLife

Monolayer-crystal streptavidin support films provide an internal standard of cryo-EM image quality

(2017) Bong-Gyoon Han et al.   JOURNAL OF STRUCTURAL BIOLOGY

Blotting-free and lossless cryo-electron microscopy grid preparation from nanoliter-sized protein samples and single-cell extracts

(2017) Stefan A. Arnold et al.   JOURNAL OF STRUCTURAL BIOLOGY

MotionCor2: anisotropic correction of beam-induced motion for improved cryo-electron microscopy

(2017) Shawn Q Zheng et al.   NATURE METHODS

cryoSPARC: algorithms for rapid unsupervised cryo-EM structure determination

(2017) Ali Punjani et al.   NATURE METHODS

A threonine turnstile defines a dynamic amphiphilic binding motif in the AAA ATPase p97 allosteric binding site

(2017) James C. Burnett et al.   ORGANIC & BIOMOLECULAR CHEMISTRY

UCSF ChimeraX: Meeting modern challenges in visualization and analysis

(2017) Thomas D. Goddard et al.   PROTEIN SCIENCE

Origin and Functional Evolution of the Cdc48/p97/VCP AAA+ Protein Unfolding and Remodeling Machine

(2016) Dominik Barthelme et al.   JOURNAL OF MOLECULAR BIOLOGY

Gctf: Real-time CTF determination and correction

(2016) Kai Zhang   JOURNAL OF STRUCTURAL BIOLOGY

A new method for vitrifying samples for cryoEM

(2016) Ivan Razinkov et al.   JOURNAL OF STRUCTURAL BIOLOGY

Nonfouling NTA-PEG-Based TEM Grid Coatings for Selective Capture of Histidine-Tagged Protein Targets from Cell Lysates

(2016) Christopher J. Benjamin et al.   LANGMUIR

2.3 A resolution cryo-EM structure of human p97 and mechanism of allosteric inhibition

(2016) S. Banerjee et al.   SCIENCE

Structural Basis of ATP Hydrolysis and Intersubunit Signaling in the AAA+ ATPase p97

(2016) Petra Hänzelmann et al.   STRUCTURE

Selective Capture of Histidine-tagged Proteins from Cell Lysates Using TEM grids Modified with NTA-Graphene Oxide

(2016) Christopher J. Benjamin et al.   Scientific Reports

Automated structure refinement of macromolecular assemblies from cryo-EM maps using Rosetta

(2016) Ray Yu-Ruei Wang et al.   eLife

Targeting the AAA ATPase p97 as an Approach to Treat Cancer through Disruption of Protein Homeostasis

(2015) Daniel J. Anderson et al.   CANCER CELL

Clipping or Extracting: Two Ways to Membrane Protein Degradation

(2015) Dönem Avci et al.   TRENDS IN CELL BIOLOGY

Structure–Activity Study of Bioisosteric Trifluoromethyl and Pentafluorosulfanyl Indole Inhibitors of the AAA ATPase p97

(2015) Celeste Alverez et al.   ACS Medicinal Chemistry Letters

Specific Inhibition of p97/VCP ATPase and Kinetic Analysis Demonstrate Interaction between D1 and D2 ATPase Domains

(2014) Tsui-Fen Chou et al.   JOURNAL OF MOLECULAR BIOLOGY

Single-step antibody-based affinity cryo-electron microscopy for imaging and structural analysis of macromolecular assemblies

(2014) Guimei Yu et al.   JOURNAL OF STRUCTURAL BIOLOGY

Structure-Activity Relationship Study Reveals ML240 and ML241 as Potent and Selective Inhibitors of p97 ATPase

(2013) Tsui-Fen Chou et al.   ChemMedChem

Covalent and allosteric inhibitors of the ATPase VCP/p97 induce cancer cell death

(2013) Paola Magnaghi et al.   Nature Chemical Biology

Alkylsulfanyl-1,2,4-triazoles, a New Class of Allosteric Valosine Containing Protein Inhibitors. Synthesis and Structure–Activity Relationships

(2012) Paolo Polucci et al.   JOURNAL OF MEDICINAL CHEMISTRY

Affinity grid-based cryo-EM of PKC binding to RACK1 on the ribosome

(2012) Gyanesh Sharma et al.   JOURNAL OF STRUCTURAL BIOLOGY

Development of p97 AAA ATPase inhibitors

(2011) Tsui-Fen Chou et al.   Autophagy

Critical Role of VCP/p97 in the Pathogenesis and Progression of Non-Small Cell Lung Carcinoma

(2011) Christopher W. Valle et al.   PLoS One

Reversible inhibitor of p97, DBeQ, impairs both ubiquitin-dependent and autophagic protein clearance pathways

(2011) T.-F. Chou et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Strategy for the Use of Affinity Grids to Prepare Non-His-Tagged Macromolecular Complexes for Single-Particle Electron Microscopy

(2010) Deborah F. Kelly et al.   JOURNAL OF MOLECULAR BIOLOGY

The Affinity Grid: A Pre-fabricated EM Grid for Monolayer Purification

(2008) Deborah F. Kelly et al.   JOURNAL OF MOLECULAR BIOLOGY

Monolayer purification: A rapid method for isolating protein complexes for single-particle electron microscopy

(2008) D. F. Kelly et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Improved Structures of Full-Length p97, an AAA ATPase: Implications for Mechanisms of Nucleotide-Dependent Conformational Change

(2008) Jason M. Davies et al.   STRUCTURE