Recent Advances in Metabolic Engineering, Protein Engineering, and Transcriptome-Guided Insights Toward Synthetic Production of Taxol

Biochemical characterization of acyl activating enzymes for side chainmoieties of taxol and its analogs

(2020) Narayanan Srividya et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

A Novel Hydroxylation Step in the Taxane Biosynthetic Pathway: A New Approach to Paclitaxel Production by Synthetic Biology

(2020) Raul Sanchez-Muñoz et al.   Frontiers in Bioengineering and Biotechnology

Rerouting plant terpene biosynthesis enables momilactone pathway elucidation

(2020) Ricardo De La Peña et al.   Nature Chemical Biology

Transcriptome analyses provide insights into the expression pattern and sequence similarity of several taxol biosynthesis-related genes in three Taxus species

(2019) Ting Zhou et al.   BMC PLANT BIOLOGY

Metabolic Engineering of Bacillus subtilis Toward Taxadiene Biosynthesis as the First Committed Step for Taxol Production

(2019) Ingy I. Abdallah et al.   Frontiers in Microbiology

Iso-Seq analysis of the Taxus cuspidata transcriptome reveals the complexity of Taxol biosynthesis

(2019) Xuejun Kuang et al.   BMC PLANT BIOLOGY

QM/MM study of the taxadiene synthase mechanism

(2019) Jeaphianne P. M. van Rijn et al.   JOURNAL OF COMPUTATIONAL CHEMISTRY

Chloroplastic metabolic engineering coupled with isoprenoid pool enhancement for committed taxanes biosynthesis in Nicotiana benthamiana

(2019) Jianhua Li et al.   Nature Communications

Slow-Starter Enzymes: Role of Active-Site Architecture in the Catalytic Control of the Biosynthesis of Taxadiene by Taxadiene Synthase

(2018) Tamar Ansbacher et al.   BIOCHEMISTRY

Enhanced catalytic activities and modified substrate preferences for taxoid 10β-O-acetyl transferase mutants by engineering catalytic histidine residues

(2018) Lin-Feng You et al.   BIOTECHNOLOGY LETTERS

Molecular dynamics study of taxadiene synthase catalysis

(2018) Andrés M. Escorcia et al.   JOURNAL OF COMPUTATIONAL CHEMISTRY

Bio-production of Baccatin III, an Important Precursor of Paclitaxel by a Cost-Effective Approach

(2018) Shu-Ling Lin et al.   MOLECULAR BIOTECHNOLOGY

Transcriptome profiling reveals specific patterns of paclitaxel synthesis in a new Taxus yunnanensis cultivar

(2018) Chun-Tao He et al.   PLANT PHYSIOLOGY AND BIOCHEMISTRY

Transcriptome-wide identification and screening of WRKY factors involved in the regulation of taxol biosynthesis in Taxus chinensis

(2018) Meng Zhang et al.   Scientific Reports

TcMYC2a, a Basic Helix–Loop–Helix Transcription Factor, Transduces JA-Signals and Regulates Taxol Biosynthesis in Taxus chinensis

(2018) Meng Zhang et al.   Frontiers in Plant Science

Transcriptome analysis of a taxol-producing endophytic fungus Cladosporium cladosporioides MD2

(2018) Li-Yun Miao et al.   AMB Express

Identification of early jasmonate-responsive genes in Taxus × media cells by analyzing time series digital gene expression data

(2018) Rongjia Mao et al.   PHYSIOLOGY AND MOLECULAR BIOLOGY OF PLANTS

Engineered bidirectional promoters enable rapid multi-gene co-expression optimization

(2018) Thomas Vogl et al.   Nature Communications

Heterologous expression and characterization of plant Taxadiene-5α-Hydroxylase (CYP725A4) in Escherichia coli

(2017) John Edward Rouck et al.   PROTEIN EXPRESSION AND PURIFICATION

Improving 10-deacetylbaccatin III-10-β-O-acetyltransferase catalytic fitness for Taxol production

(2017) Bing-Juan Li et al.   Nature Communications

Catalytic Control in the Facile Proton Transfer in Taxadiene Synthase

(2017) Yehoshua Freud et al.   ACS Catalysis

Production of taxadiene by engineering of mevalonate pathway in Escherichia coli and endophytic fungus Alternaria alternata TPF6

(2017) Guangkai Bian et al.   Biotechnology Journal

Transcriptome Assembly and Systematic Identification of Novel Cytochrome P450s in Taxus chinensis

(2017) Weifang Liao et al.   Frontiers in Plant Science

Orthogonal Assays Clarify the Oxidative Biochemistry of Taxol P450 CYP725A4

(2016) Bradley Walters Biggs et al.   ACS Chemical Biology

A Cas9-based toolkit to program gene expression inSaccharomyces cerevisiae

(2016) Amanda Reider Apel et al.   NUCLEIC ACIDS RESEARCH

Overcoming heterologous protein interdependency to optimize P450-mediated Taxol precursor synthesis inEscherichia coli

(2016) Bradley Walters Biggs et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Identification of amino acid networks governing catalysis in the closed complex of class I terpene synthases

(2016) Patrick Schrepfer et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Accessing low-oxidation state taxanes: is taxadiene-4(5)-epoxide on the taxol biosynthetic pathway?

(2016) Naomi A. Barton et al.   Chemical Science

Engineering of Taxadiene Synthase for Improved Selectivity and Yield of a Key Taxol Biosynthetic Intermediate

(2016) Steven Edgar et al.   ACS Synthetic Biology

Mechanistic Insights into Taxadiene Epoxidation by Taxadiene-5α-Hydroxylase

(2015) Steven Edgar et al.   ACS Chemical Biology

Distributing a metabolic pathway among a microbial consortium enhances production of natural products

(2015) Kang Zhou et al.   NATURE BIOTECHNOLOGY

Transcript profiling of jasmonate-elicited Taxus cells reveals a β-phenylalanine-CoA ligase

(2015) Karla Ramírez-Estrada et al.   PLANT BIOTECHNOLOGY JOURNAL

Jasmonate-responsive expression of paclitaxel biosynthesis genes in Taxus cuspidata cultured cells is negatively regulated by the bHLH transcription factors TcJAMYC1, TcJAMYC2, and TcJAMYC4

(2015) Sangram K. Lenka et al.   Frontiers in Plant Science

Nature as a remarkable chemist

(2014) Mansukh C. Wani et al.   ANTI-CANCER DRUGS

Unraveling the multispecificity and catalytic promiscuity of taxadiene monooxygenase

(2014) Vikramaditya G. Yadav   JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC

Biosynthesis of Taxadiene in Saccharomyces cerevisiae : Selection of Geranylgeranyl Diphosphate Synthase Directed by a Computer-Aided Docking Strategy

(2014) Ming-zhu Ding et al.   PLoS One

Production of taxadiene from cultured ginseng roots transformed with taxadiene synthase gene

(2012) Mi-Jeong Cha et al.   BMB Reports

The Taxadiene-Forming Carbocation Cascade

(2011) Young J. Hong et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Taxadiene synthase structure and evolution of modular architecture in terpene biosynthesis

(2010) Mustafa Köksal et al.   NATURE

Production of the anticancer drug taxol in Taxus baccata suspension cultures: A review

(2010) Sonia Malik et al.   PROCESS BIOCHEMISTRY

Isoprenoid Pathway Optimization for Taxol Precursor Overproduction in Escherichia coli

(2010) P. K. Ajikumar et al.   SCIENCE

Production of taxa-4(5),11(12)-diene by transgenic Physcomitrella patens

(2009) Aldwin Anterola et al.   TRANSGENIC RESEARCH

Metabolic engineering of taxadiene biosynthesis in yeast as a first step towards Taxol (Paclitaxel) production

(2008) Benedikt Engels et al.   METABOLIC ENGINEERING