Base editing and prime editing in laboratory animals

Genome editing methods in animal models

(2020) Hyunji Lee et al.   Animal Cells and Systems

In situ CRISPR‐Cas9 base editing for the development of genetically engineered mouse models of breast cancer

(2020) Stefano Annunziato et al.   EMBO JOURNAL

Treatment of a Mouse Model of ALS by In Vivo Base Editing

(2020) Colin K.W. Lim et al.   MOLECULAR THERAPY

Efficient base editing with high precision in rabbits using YFE-BE4max

(2020) Zhiquan Liu et al.   Cell Death & Disease

Cytosine and adenine base editing of the brain, liver, retina, heart and skeletal muscle of mice via adeno-associated viruses

(2020) Jonathan M. Levy et al.   Nature Biomedical Engineering

Dual base editor catalyzes both cytosine and adenine base conversions in human cells

(2020) Xiaohui Zhang et al.   NATURE BIOTECHNOLOGY

A dual-deaminase CRISPR base editor enables concurrent adenine and cytosine editing

(2020) Julian Grünewald et al.   NATURE BIOTECHNOLOGY

Base editors for simultaneous introduction of C-to-T and A-to-G mutations

(2020) Rina C. Sakata et al.   NATURE BIOTECHNOLOGY

Chemical modifications of adenine base editor mRNA and guide RNA expand its application scope

(2020) Tingting Jiang et al.   Nature Communications

Multiplex precise base editing in cynomolgus monkeys

(2020) Wenhui Zhang et al.   Nature Communications

Efficient generation of mouse models with the prime editing system

(2020) Yao Liu et al.   Cell Discovery

Genome editing with CRISPR–Cas nucleases, base editors, transposases and prime editors

(2020) Andrew V. Anzalone et al.   NATURE BIOTECHNOLOGY

CRISPR C-to-G base editors for inducing targeted DNA transversions in human cells

(2020) Ibrahim C. Kurt et al.   NATURE BIOTECHNOLOGY

In vivo base editing restores sensory transduction and transiently improves auditory function in a mouse model of recessive deafness

(2020) Wei-Hsi Yeh et al.   Science Translational Medicine

Cas12a Base Editors Induce Efficient and Specific Editing with Low DNA Damage Response

(2020) Xiao Wang et al.   Cell Reports

Generation of a Hutchinson–Gilford progeria syndrome monkey model by base editing

(2020) Fang Wang et al.   Protein & Cell

BE4max and AncBE4max Are Efficient in Germline Conversion of C:G to T:A Base Pairs in Zebrafish

(2020) Blake Carrington et al.   Cells

In vivo genome and base editing of a human PCSK9 knock-in hypercholesterolemic mouse model

(2019) Alba Carreras et al.   BMC BIOLOGY

Simultaneous targeting of linked loci in mouse embryos using base editing

(2019) Hye Kyung Lee et al.   Scientific Reports

CRISPR-Cas9 genome editing induces megabase-scale chromosomal truncations

(2019) Grégoire Cullot et al.   Nature Communications

Adeno-associated virus vector as a platform for gene therapy delivery

(2019) Dan Wang et al.   NATURE REVIEWS DRUG DISCOVERY

Genome-wide target specificity of CRISPR RNA-guided adenine base editors

(2019) Daesik Kim et al.   NATURE BIOTECHNOLOGY

Adenine base editing in an adult mouse model of tyrosinaemia

(2019) Chun-Qing Song et al.   Nature Biomedical Engineering

Transcriptome-wide off-target RNA editing induced by CRISPR-guided DNA base editors

(2019) Julian Grünewald et al.   NATURE

Analysis and minimization of cellular RNA editing by DNA adenine base editors

(2019) Holly A. Rees et al.   Science Advances

Efficient base editing by RNA-guided cytidine base editors (CBEs) in pigs

(2019) Hongming Yuan et al.   CELLULAR AND MOLECULAR LIFE SCIENCES

Off-target RNA mutation induced by DNA base editing and its elimination by mutagenesis

(2019) Changyang Zhou et al.   NATURE

Efficient base editing for multiple genes and loci in pigs using base editors

(2019) Jingke Xie et al.   Nature Communications

Adenine base editors catalyze cytosine conversions in human cells

(2019) Heon Seok Kim et al.   NATURE BIOTECHNOLOGY

Search-and-replace genome editing without double-strand breaks or donor DNA

(2019) Andrew V. Anzalone et al.   NATURE

Adenine base editing in mouse embryos and an adult mouse model of Duchenne muscular dystrophy

(2018) Seuk-Min Ryu et al.   NATURE BIOTECHNOLOGY

Repair of double-strand breaks induced by CRISPR–Cas9 leads to large deletions and complex rearrangements

(2018) Michael Kosicki et al.   NATURE BIOTECHNOLOGY

Improving cytidine and adenine base editors by expression optimization and ancestral reconstruction

(2018) Luke W Koblan et al.   NATURE BIOTECHNOLOGY

Optimized base editors enable efficient editing in cells, organoids and mice

(2018) Maria Paz Zafra et al.   NATURE BIOTECHNOLOGY

In vivo base editing of post-mitotic sensory cells

(2018) Wei-Hsi Yeh et al.   Nature Communications

Highly efficient RNA-guided base editing in rabbit

(2018) Zhiquan Liu et al.   Nature Communications

Efficient generation of mouse models of human diseases via ABE- and BE-mediated base editing

(2018) Zhen Liu et al.   Nature Communications

Introduction of pathogenic mutations into the mouse Psen1 gene by Base Editor and Target-AID

(2018) Hiroki Sasaguri et al.   Nature Communications

Highly efficient and precise base editing by engineered dCas9-guide tRNA adenosine deaminase in rats

(2018) Yuanwu Ma et al.   Cell Discovery

Evolved Cas9 variants with broad PAM compatibility and high DNA specificity

(2018) Johnny H. Hu et al.   NATURE

An APOBEC3A-Cas9 base editor with minimized bystander and off-target activities

(2018) Jason M Gehrke et al.   NATURE BIOTECHNOLOGY

CRISPR–Cas9-mediated base-editing screening in mice identifies DND1 amino acids that are critical for primordial germ cell development

(2018) Qing Li et al.   NATURE CELL BIOLOGY

Engineered CRISPR-Cas9 nuclease with expanded targeting space

(2018) Hiroshi Nishimasu et al.   SCIENCE

In vivo targeted single-nucleotide editing in zebrafish

(2018) Shingo Tanaka et al.   Scientific Reports

Effective and precise adenine base editing in mouse zygotes

(2018) Puping Liang et al.   Protein & Cell

Increasing targeting scope of adenosine base editors in mouse and rat embryos through fusion of TadA deaminase with Cas9 variants

(2018) Lei Yang et al.   Protein & Cell

Precise A•T to G•C base editing in the zebrafish genome

(2018) Wei Qin et al.   BMC BIOLOGY

Treatment of a metabolic liver disease by in vivo genome base editing in adult mice

(2018) Lukas Villiger et al.   NATURE MEDICINE

In utero CRISPR-mediated therapeutic editing of metabolic genes

(2018) Avery C. Rossidis et al.   NATURE MEDICINE

Base editing: precision chemistry on the genome and transcriptome of living cells

(2018) Holly A. Rees et al.   NATURE REVIEWS GENETICS

Targeting fidelity of adenine and cytosine base editors in mouse embryos

(2018) Hye Kyung Lee et al.   Nature Communications

CRISPR-Cas orthologues and variants: optimizing the repertoire, specificity and delivery of genome engineering tools

(2017) Alberto Cebrian-Serrano et al.   MAMMALIAN GENOME

Programmable base editing of A•T to G•C in genomic DNA without DNA cleavage

(2017) Nicole M. Gaudelli et al.   NATURE

Increasing the genome-targeting scope and precision of base editing with engineered Cas9-cytidine deaminase fusions

(2017) Y Bill Kim et al.   NATURE BIOTECHNOLOGY

Highly efficient RNA-guided base editing in mouse embryos

(2017) Kyoungmi Kim et al.   NATURE BIOTECHNOLOGY

Genome-wide target specificities of CRISPR RNA-guided programmable deaminases

(2017) Daesik Kim et al.   NATURE BIOTECHNOLOGY

CRISPR/Cas9 targeting events cause complex deletions and insertions at 17 sites in the mouse genome

(2017) Ha Youn Shin et al.   Nature Communications

Programmable base editing of zebrafish genome using a modified CRISPR-Cas9 system

(2017) Yihan Zhang et al.   Nature Communications

Improving the DNA specificity and applicability of base editing through protein engineering and protein delivery

(2017) Holly A. Rees et al.   Nature Communications

CRISPR/Cas9-mediated genome editing via postnatal administration of AAV vector cures haemophilia B mice

(2017) Tsukasa Ohmori et al.   Scientific Reports

Highly efficient base editing in human tripronuclear zygotes

(2017) Changyang Zhou et al.   Protein & Cell

Effective gene editing by high-fidelity base editor 2 in mouse zygotes

(2017) Puping Liang et al.   Protein & Cell

Improved base excision repair inhibition and bacteriophage Mu Gam protein yields C:G-to-T:A base editors with higher efficiency and product purity

(2017) Alexis C. Komor et al.   Science Advances

Revealing hidden complexities of genomic rearrangements generated with Cas9

(2017) Katharina Boroviak et al.   Scientific Reports

CRISPR-Cas9 Targeting ofPCSK9in Human Hepatocytes In Vivo—Brief ReportSignificance

(2016) Xiao Wang et al.   ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY

Programmable editing of a target base in genomic DNA without double-stranded DNA cleavage

(2016) Alexis C. Komor et al.   NATURE

Targeted nucleotide editing using hybrid prokaryotic and vertebrate adaptive immune systems

(2016) K. Nishida et al.   SCIENCE

Efficient CRISPR/Cas9-Mediated Genome Editing in Mice by Zygote Electroporation of Nuclease

(2015) Wenning Qin et al.   GENETICS

In vivo genome editing using Staphylococcus aureus Cas9

(2015) F. Ann Ran et al.   NATURE

Efficient CRISPR/Cas9-Mediated Genome Editing in Mice by Zygote Electroporation of Nuclease

(2015) Wenning Qin et al.   GENETICS

One-Step Generation of Mice Carrying Mutations in Multiple Genes by CRISPR/Cas-Mediated Genome Engineering

(2013) Haoyi Wang et al.   CELL

ClinVar: public archive of relationships among sequence variation and human phenotype

(2013) Melissa J. Landrum et al.   NUCLEIC ACIDS RESEARCH

Multiplex Genome Engineering Using CRISPR/Cas Systems

(2013) L. Cong et al.   SCIENCE

RNA-programmed genome editing in human cells

(2013) Martin Jinek et al.   eLife

Playing the End Game: DNA Double-Strand Break Repair Pathway Choice

(2012) J. Ross Chapman et al.   MOLECULAR CELL

The AID/APOBEC family of nucleic acid mutators

(2008) Silvestro G Conticello   GENOME BIOLOGY