CRISPR/Cas9 targeting events cause complex deletions and insertions at 17 sites in the mouse genome

The Heroes of CRISPR

(2016) Eric S. Lander   CELL

Dynamic Control of Enhancer Repertoires Drives Lineage and Stage-Specific Transcription during Hematopoiesis

(2016) Jialiang Huang et al.   DEVELOPMENTAL CELL

CRISPR/Cas9‐mediated somatic correction of a novel coagulator factor IX gene mutation ameliorates hemophilia in mouse

(2016) Yuting Guan et al.   EMBO Molecular Medicine

CTCF: making the right connections

(2016) Rodolfo Ghirlando et al.   GENES & DEVELOPMENT

A possible aid in targeted insertion of large DNA elements by CRISPR/Cas in mouse zygotes

(2016) Harumi Nakao et al.   GENESIS

Chromosome engineering in zygotes with CRISPR/Cas9

(2016) Katharina Boroviak et al.   GENESIS

Exome sequencing and CRISPR/Cas genome editing identify mutations of ZAK as a cause of limb defects in humans and mice

(2016) Malte Spielmann et al.   GENOME RESEARCH

A new class of temporarily phenotypic enhancers identified by CRISPR/Cas9-mediated genetic screening

(2016) Yarui Diao et al.   GENOME RESEARCH

Heterozygous mutation ofUsh1g/Sansin mice causes early-onset progressive hearing loss, which is recovered by reconstituting the strain-specific mutation inCdh23

(2016) Yuki Miyasaka et al.   HUMAN MOLECULAR GENETICS

Microinjection-based generation of mutant mice with a double mutation and a 0.5 Mb deletion in their genome by the CRISPR/Cas9 system

(2016) Satoshi HARA et al.   JOURNAL OF REPRODUCTION AND DEVELOPMENT

Functional validation of tensin2 SH2-PTB domain by CRISPR/Cas9-mediated genome editing

(2016) Kiyoma MARUSUGI et al.   JOURNAL OF VETERINARY MEDICAL SCIENCE

Functional genetic screens for enhancer elements in the human genome using CRISPR-Cas9

(2016) Gozde Korkmaz et al.   NATURE BIOTECHNOLOGY

Hierarchy within the mammary STAT5-driven Wap super-enhancer

(2016) Ha Youn Shin et al.   NATURE GENETICS

Creating and evaluating accurate CRISPR-Cas9 scalpels for genomic surgery

(2016) Mehmet Fatih Bolukbasi et al.   NATURE METHODS

Ligase I and ligase III mediate the DNA double-strand break ligation in alternative end-joining

(2016) Guangqing Lu et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Efficient generation of FVII gene knockout mice using CRISPR/Cas9 nuclease and truncated guided RNAs

(2016) Liyou An et al.   Scientific Reports

Lineage-Specific and Non-specific Cytokine-Sensing Genes Respond Differentially to the Master Regulator STAT5

(2016) Xianke Zeng et al.   Cell Reports

Deciphering relationship between microhomology and in-frame mutation occurrence in human CRISPR-based gene knockout

(2016) Guohui Chuai et al.   Molecular Therapy-Nucleic Acids

CRISPR/Cas9-mediated gene knockout in the mouse brain using in utero electroporation

(2016) Yohei Shinmyo et al.   Scientific Reports

Endothelin signaling activates Mef2c expression in the neural crest through a MEF2C-dependent positive-feedback transcriptional pathway

(2015) J. Hu et al.   DEVELOPMENT

Disruption of MeCP2 attenuates circadian rhythm in CRISPR/Cas9-based Rett syndrome model mouse

(2015) Yoshiki Tsuchiya et al.   GENES TO CELLS

Efficient CRISPR/Cas9-Mediated Genome Editing in Mice by Zygote Electroporation of Nuclease

(2015) Wenning Qin et al.   GENETICS

CRISPR/Cas9-Mediated Rapid Generation of Multiple Mouse Lines Identified Ccdc63 as Essential for Spermiogenesis

(2015) Samantha Young et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Efficient ligase 3-dependent microhomology-mediated end joining repair of DNA double-strand breaks in zebrafish embryos

(2015) Mu-Dan He et al.   MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS

Increasing the efficiency of precise genome editing with CRISPR-Cas9 by inhibition of nonhomologous end joining

(2015) Takeshi Maruyama et al.   NATURE BIOTECHNOLOGY

An autoregulatory enhancer controls mammary-specific STAT5 functions

(2015) Gil Metser et al.   NUCLEIC ACIDS RESEARCH

Functional validation of mouse tyrosinase non-coding regulatory DNA elements by CRISPR–Cas9-mediated mutagenesis

(2015) Davide Seruggia et al.   NUCLEIC ACIDS RESEARCH

CRISPR-Cas9-Mediated Genome Editing in Leishmania donovani

(2015) Wen-Wei Zhang et al.   mBio

Efficient inversions and duplications of mammalian regulatory DNA elements and gene clusters by CRISPR/Cas9

(2015) Jinhuan Li et al.   Journal of Molecular Cell Biology

Homology-directed repair in rodent zygotes using Cas9 and TALEN engineered proteins

(2015) Séverine Ménoret et al.   Scientific Reports

Large genomic fragment deletion and functional gene cassette knock-in via Cas9 protein mediated genome editing in one-cell rodent embryos

(2015) Liren Wang et al.   Scientific Reports

Efficient CRISPR/Cas9-Mediated Genome Editing in Mice by Zygote Electroporation of Nuclease

(2015) Wenning Qin et al.   GENETICS

Large Genomic Fragment Deletions and Insertions in Mouse Using CRISPR/Cas9

(2015) Luqing Zhang et al.   PLoS One

Somatic mosaicism and allele complexity induced by CRISPR/Cas9 RNA injections in mouse zygotes

(2014) Shuo-Ting Yen et al.   DEVELOPMENTAL BIOLOGY

Dual sgRNAs facilitate CRISPR/Cas9-mediated mouse genome targeting

(2014) Jiankui Zhou et al.   FEBS Journal

Zinc finger nuclease: a new approach for excising HIV-1 proviral DNA from infected human T cells

(2014) Xiying Qu et al.   MOLECULAR BIOLOGY REPORTS

Chromosomal Translocations in Human Cells Are Generated by Canonical Nonhomologous End-Joining

(2014) Hind Ghezraoui et al.   MOLECULAR CELL

Coregulation of Genetic Programs by the Transcription Factors NFIB and STAT5

(2014) Gertraud W. Robinson et al.   MOLECULAR ENDOCRINOLOGY

Microhomology-based choice of Cas9 nuclease target sites

(2014) Sangsu Bae et al.   NATURE METHODS

CRISPR Reveals a Distal Super-Enhancer Required for Sox2 Expression in Mouse Embryonic Stem Cells

(2014) Yan Li et al.   PLoS One

CRISPR-Cas9-Mediated Single-Gene and Gene Family Disruption in Trypanosoma cruzi

(2014) D. Peng et al.   mBio

Validation of microinjection methods for generating knockout mice by CRISPR/Cas-mediated genome engineering

(2014) Takuro Horii et al.   Scientific Reports

One-Step Generation of Mice Carrying Mutations in Multiple Genes by CRISPR/Cas-Mediated Genome Engineering

(2013) Haoyi Wang et al.   CELL

One-Step Generation of Mice Carrying Reporter and Conditional Alleles by CRISPR/Cas-Mediated Genome Engineering

(2013) Hui Yang et al.   CELL

Generation of gene-modified mice via Cas9/RNA-mediated gene targeting

(2013) Bin Shen et al.   CELL RESEARCH

Feasibility for a large scale mouse mutagenesis by injecting CRISPR/Cas plasmid into zygotes

(2013) Daisuke Mashiko et al.   DEVELOPMENT GROWTH & DIFFERENTIATION

Genome Engineering of Drosophila with the CRISPR RNA-Guided Cas9 Nuclease

(2013) Scott J. Gratz et al.   GENETICS

Highly efficient gene knockout in mice and zebrafish with RNA-guided endonucleases

(2013) Y. H. Sung et al.   GENOME RESEARCH

NFIB is a governor of epithelial–melanocyte stem cell behaviour in a shared niche

(2013) Chiung-Ying Chang et al.   NATURE

Efficient generation of large-scale genome-modified mice using gRNA and CAS9 endonuclease

(2013) Wataru Fujii et al.   NUCLEIC ACIDS RESEARCH

RNA-Guided Human Genome Engineering via Cas9

(2013) P. Mali et al.   SCIENCE

Multiplex Genome Engineering Using CRISPR/Cas Systems

(2013) L. Cong et al.   SCIENCE

RNA-programmed genome editing in human cells

(2013) Martin Jinek et al.   eLife

A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity

(2012) M. Jinek et al.   SCIENCE

Interference by clustered regularly interspaced short palindromic repeat (CRISPR) RNA is governed by a seed sequence

(2011) E. Semenova et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

MMEJ repair of double-strand breaks (director’s cut): deleted sequences and alternative endings

(2008) Mitch McVey et al.   TRENDS IN GENETICS