Multiattribute Monitoring of Antibody Charge Variants by Cation-Exchange Chromatography Coupled to Native Mass Spectrometry

Antibodies to watch in 2021

(2021) Hélène Kaplon et al.   mAbs

Multi-Attribute Method for Quality Control of Therapeutic Proteins

(2019) Sarah Rogstad et al.   ANALYTICAL CHEMISTRY

Antibodies to watch in 2020

(2019) Hélène Kaplon et al.   mAbs

Antibodies to watch in 2018

(2018) Hélène Kaplon et al.   mAbs

Ultrasensitive Characterization of Charge Heterogeneity of Therapeutic Monoclonal Antibodies Using Strong Cation Exchange Chromatography Coupled to Native Mass Spectrometry

(2018) Yuetian Yan et al.   ANALYTICAL CHEMISTRY

Antibodies to watch in 2019

(2018) Hélène Kaplon et al.   mAbs

Charge variant native mass spectrometry benefits mass precision and dynamic range of monoclonal antibody intact mass analysis

(2018) Aaron O. Bailey et al.   mAbs

Comprehensive characterisation of the heterogeneity of adalimumab via charge variant analysis hyphenated on-line to native high resolution Orbitrap mass spectrometry

(2018) Florian Füssl et al.   mAbs

Development of a fast workflow to screen the charge variants of therapeutic antibodies

(2017) Elsa Wagner-Rousset et al.   JOURNAL OF CHROMATOGRAPHY A

LC-MS/MS-Based Monitoring of In Vivo Protein Biotransformation: Quantitative Determination of Trastuzumab and Its Deamidation Products in Human Plasma

(2016) Peter Bults et al.   ANALYTICAL CHEMISTRY

Characterization of Chain Pairing Variants of Bispecific IgG Expressed in a Single Host Cell by High-Resolution Native and Denaturing Mass Spectrometry

(2016) Luis Schachner et al.   ANALYTICAL CHEMISTRY

A Novel Carcinoembryonic Antigen T-Cell Bispecific Antibody (CEA TCB) for the Treatment of Solid Tumors

(2016) M. Bacac et al.   CLINICAL CANCER RESEARCH

CEA TCB: A novel head-to-tail 2:1 T cell bispecific antibody for treatment of CEA-positive solid tumors

(2016) Marina Bacac et al.   OncoImmunology

Fc glycans of therapeutic antibodies as critical quality attributes

(2015) Dietmar Reusch et al.   GLYCOBIOLOGY

Functional assessment of antibody oxidation by native mass spectrometry

(2015) Markus Haberger et al.   mAbs

Rapid characterization of biotherapeutic proteins by size-exclusion chromatography coupled to native mass spectrometry

(2015) Markus Haberger et al.   mAbs

Development of a quantitative mass spectrometry multi-attribute method for characterization, quality control testing and disposition of biologics

(2015) Richard S Rogers et al.   mAbs

O-Glycosylation of glycine-serine linkers in recombinant Fc-fusion proteins

(2014) Chris Spahr et al.   mAbs

Assessment of chemical modifications of sites in the CDRs of recombinant antibodies

(2014) Markus Haberger et al.   mAbs

Detailed mass analysis of structural heterogeneity in monoclonal antibodies using native mass spectrometry

(2014) Sara Rosati et al.   Nature Protocols

Discovery and Investigation of O-Xylosylation in Engineered Proteins Containing a (GGGGS)n Linker

(2013) Dingyi Wen et al.   ANALYTICAL CHEMISTRY

O-xylosylation in a Recombinant Protein is Directed at a Common Motif on Glycine–Serine Linkers

(2013) David Spencer et al.   JOURNAL OF PHARMACEUTICAL SCIENCES

Complex mixtures of antibodies generated from a single production qualitatively and quantitatively evaluated by native Orbitrap mass spectrometry

(2013) Natalie J Thompson et al.   mAbs

Qualitative and Semiquantitative Analysis of Composite Mixtures of Antibodies by Native Mass Spectrometry

(2012) Sara Rosati et al.   ANALYTICAL CHEMISTRY

Characterization of Therapeutic Antibodies and Related Products

(2012) Alain Beck et al.   ANALYTICAL CHEMISTRY

Biosimilar, Biobetter, and Next Generation Antibody Characterization by Mass Spectrometry

(2012) Alain Beck et al.   ANALYTICAL CHEMISTRY

Native ion mobility-mass spectrometry and related methods in structural biology

(2012) A. Konijnenberg et al.   BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS

Progress in overcoming the chain association issue in bispecific heterodimeric IgG antibodies

(2012) Christian Klein et al.   mAbs

Simultaneous Assessment of Asp Isomerization and Asn Deamidation in Recombinant Antibodies by LC-MS following Incubation at Elevated Temperatures

(2012) Katharina Diepold et al.   PLoS One

Novel protein scaffolds as emerging therapeutic proteins: from discovery to clinical proof-of-concept

(2012) Thierry Wurch et al.   TRENDS IN BIOTECHNOLOGY

Characterization of Site-Specific Glycation During Process Development of a Human Therapeutic Monoclonal Antibody

(2011) Amanda K. Miller et al.   JOURNAL OF PHARMACEUTICAL SCIENCES

Therapeutic Fc-fusion proteins and peptides as successful alternatives to antibodies

(2011) Alain Beck et al.   mAbs

Identification of Potential Sites for Tryptophan Oxidation in Recombinant Antibodies Using tert-Butylhydroperoxide and Quantitative LC-MS

(2011) Miriam Hensel et al.   PLoS One

Immunoglobulin domain crossover as a generic approach for the production of bispecific IgG antibodies

(2011) W. Schaefer et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Quantitative Analysis of the Interaction Strength and Dynamics of Human IgG4 Half Molecules by Native Mass Spectrometry

(2011) Rebecca J. Rose et al.   STRUCTURE

Assessing monoclonal antibody product quality attribute criticality through clinical studies

(2010) Andrew M. Goetze et al.   mAbs

Naturally occurring glycan forms of human immunoglobulins G1 and G2

(2010) Gregory C. Flynn et al.   MOLECULAR IMMUNOLOGY

Mass Spectrometric Analysis of Intact Human Monoclonal Antibody Aggregates Fractionated by Size-Exclusion Chromatography

(2010) Başak Kükrer et al.   PHARMACEUTICAL RESEARCH

Identification and characterization of asparagine deamidation in the light chain CDR1 of a humanized IgG1 antibody

(2009) Josef Vlasak et al.   ANALYTICAL BIOCHEMISTRY

Extending Mass Spectrometry Contribution to Therapeutic Monoclonal Antibody Lead Optimization: Characterization of Immune Complexes Using Noncovalent ESI-MS

(2009) Cédric Atmanene et al.   ANALYTICAL CHEMISTRY

Asparagine-linked Oligosaccharides Present on a Non-consensus Amino Acid Sequence in the CH1 Domain of Human Antibodies

(2009) John F. Valliere-Douglass et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Label-free determination of protein-ligand binding constants using mass spectrometry and validation using surface plasmon resonance and isothermal titration calorimetry

(2009) Matthias C. Jecklin et al.   JOURNAL OF MOLECULAR RECOGNITION

Succinimide formation at Asn 55 in the complementarity determining region of a recombinant monoclonal antibody IgG1 heavy chain

(2009) Boxu Yan et al.   JOURNAL OF PHARMACEUTICAL SCIENCES

Impact of methionine oxidation on the binding of human IgG1 to FcRn and Fcγ receptors

(2009) Andrea Bertolotti-Ciarlet et al.   MOLECULAR IMMUNOLOGY

Unveiling a Glycation Hot Spot in a Recombinant Humanized Monoclonal Antibody

(2008) Boyan Zhang et al.   ANALYTICAL CHEMISTRY

Isomerization of a Single Aspartyl Residue of Anti-Epidermal Growth Factor Receptor Immunoglobulin γ2 Antibody Highlights the Role Avidity Plays in Antibody Activity

(2008) Douglas S. Rehder et al.   BIOCHEMISTRY

Trends in Glycosylation, Glycoanalysis and Glycoengineering of Therapeutic Antibodies and Fc-Fusion Proteins

(2008) Alain Beck et al.   CURRENT PHARMACEUTICAL BIOTECHNOLOGY

Heterogeneity of Monoclonal Antibodies Revealed by Charge-Sensitive Methods

(2008) J. Vlasak et al.   CURRENT PHARMACEUTICAL BIOTECHNOLOGY

Mass spectrometry for structural characterization of therapeutic antibodies

(2008) Zhongqi Zhang et al.   MASS SPECTROMETRY REVIEWS

Native mass spectrometry: a bridge between interactomics and structural biology

(2008) Albert J R Heck   NATURE METHODS

A study in glycation of a therapeutic recombinant humanized monoclonal antibody: Where it is, how it got there, and how it affects charge-based behavior

(2007) Cynthia Quan et al.   ANALYTICAL BIOCHEMISTRY

Glycosylation of Recombinant Antibody Therapeutics

(2005) Royston Jefferis   BIOTECHNOLOGY PROGRESS