Article
Medicine, Research & Experimental
Elham Fakhr, Fatemeh Zare, Kayhan Azadmanesh, Ladan Teimoori-Toolabi
Summary: LEF1 plays an important role in the chemo-resistance of colorectal cancer cells, with downregulation of the long isoform making cells more sensitive to chemotherapy through reduced proliferation, increased apoptosis, or cell cycle arrest. Balancing the expression of long and short isoforms of LEF1 is crucial in overcoming chemo-resistance.
BIOMEDICINE & PHARMACOTHERAPY
(2021)
Article
Oncology
Yaoqing Li, Chuchu Xu, Renjun Zhu, Liyijing Shen, Gengyuan Hu, Kelong Tao, Feng Tao, Zengxin Lu, Guolin Zhang
Summary: This study evaluates the value of tissue inhibitors of MMPs-2 (TIMP-2) as an indicator of 5-Fluorouracil (5-Fu) resistance in colorectal cancer. The results show that TIMP-2 expression is elevated in drug-resistant colorectal cancer cell lines and is closely related to 5-Fu resistance. Monitoring serum TIMP-2 levels can help clinicians identify 5-Fu resistance in colorectal cancer patients earlier during chemotherapy.
JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY
(2023)
Article
Pathology
Yu-Ling Qu, Xiao-Li Liu, Shan-Yu Zhao, Xue-Feng Zhai
Summary: This study found that SPOCK1 is upregulated in 5-FU-resistant CRC cells and that knockdown of SPOCK1 increases their sensitivity to 5-FU. Conversely, overexpression of SPOCK1 enhances CRC cell resistance to 5-FU and reduces cell apoptosis.
PATHOLOGY RESEARCH AND PRACTICE
(2022)
Article
Biochemistry & Molecular Biology
Ninon Very, Stephan Hardiville, Amelie Decourcelle, Julien Thevenet, Madjid Djouina, Adeline Page, Gerard Vergoten, Celine Schulz, Julie Kerr-Conte, Tony Lefebvre, Vanessa Dehennaut, Ikram El Yazidi-Belkoura
Summary: Alteration of O-GlcNAcylation affects tumorigenesis and tumor progression, with a potential role in chemotherapy response. Combination treatment of 5-FU with Thiamet-G shows synergistic inhibitory effects on CRC progression by regulating TS and O-GlcNacylation levels. Overall, the findings suggest a new approach to enhance CRC response to 5-FU chemotherapy by targeting O-GlcNAcylation.
Article
Oncology
Min Li, Mingyue Xia, Ziyu Zhang, Yanyin Tan, Enjie Li, Zhigang Guo, Mingzhi Fang, Yong Zhu, Zhigang Hu
Summary: This study reveals the important role of the METTL3/RAD51AP1 axis in the acquisition of 5-FU resistance in CRC, and targeting METTL3/RAD51AP1 may be a promising adjuvant therapeutic strategy for patients with CRC, particularly for those with 5-FU-resistant CRC.
INTERNATIONAL JOURNAL OF ONCOLOGY
(2022)
Article
Anatomy & Morphology
Xueni Wei, Zhonghui Yang, Guomei Chen, Ji Huang
Summary: High expression of VMP1 in colon cancer cells can promote exosome secretion, which leads to the acquisition of more drug-resistant properties by cancer cells.
Article
Cell Biology
Huan Niu, Meng Zhao, Jing Huang, Jing Wang, Yang Si, Shan Cheng, Wei Ding
Summary: CBs may serve as indicators of drug sensitivity or resistance in tumor cells, while UHMK1 maintains the structure and morphology of CBs by regulating splicing events.
CELL COMMUNICATION AND SIGNALING
(2022)
Review
Medicine, Research & Experimental
Chinmayee Sethy, Chanakya Nath Kundu
Summary: 5-Fluorouracil (5-FU) is a crucial anti-cancer drug, but drug resistance has hindered its clinical efficacy. Overcoming chemoresistance, especially caused by cancer stem cells, remains a challenge.
BIOMEDICINE & PHARMACOTHERAPY
(2021)
Article
Biochemistry & Molecular Biology
Tsui-Chin Huang, Kuan-Chieh Peng, Tzu-Ting Kuo, Li-Chun Lin, Bai-Chia Liu, Shu-Ping Ye, Chien-Chou Chu, Shih-Min Hsia, Hsin-Yi Chang
Summary: By using pharmacogenomic analysis, this study found a correlation between APC gene mutations in colorectal cancer cells and resistance, showing synergistic effects of combining a MEK inhibitor with 5-FU on cells with APC truncations. Additionally, targeting ROCK was identified as a potential avenue to restore 5-FU response in resistant cells with wild-type APC background.
Article
Chemistry, Medicinal
Junwu Lin, Hong Qin, Yue Han, Xinghua Li, YuJuan Zhao, Guangsheng Zhai
Summary: The study revealed an increased expression of circNRIP1 in chemoresistant NPC patients, with functional interactions between circNRIP1, miR-515-5p, and IL-25. Knocking down circNRIP1 restored NPC cell sensitivity to 5-Fu and cisplatin, indicating a potential therapeutic target for treating this cancer type.
DRUG DESIGN DEVELOPMENT AND THERAPY
(2021)
Article
Biochemistry & Molecular Biology
Haili Wu, Jin'e Du, Chenglu Li, Hanqing Li, Huiqin Guo, Zhuoyu Li
Summary: Resistance to 5-Fluorouracil (5-Fu) chemotherapy is a major obstacle in colon cancer treatment. This study found that the dietary flavonoid kaempferol can reverse the drug resistance of colorectal cancer cells to 5-Fu, suggesting a potential treatment option. The mechanism behind this reversal involves the regulation of the miR-326-hnRNPA1/A2/PTBP1-PKM2 axis and inhibition of glycolysis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Pathology
Guangyi Liu, Huan Wang, Rui Ran, Yicheng Wang, Yang Li
Summary: The study investigated the molecular mechanism of FOSL1 regulating 5-FU resistance in colon cancer. It was found that high expression of FOSL1 was associated with 5-FU resistance and poor prognosis in colon cancer. Low expression of FOSL1 enhanced 5-FU sensitivity, suppressed cell proliferation, and induced apoptosis in colon cancer cells. Mechanistically, FOSL1 activated PHLDA2 and promoted glycolysis, leading to increased 5-Fu resistance and cell proliferation.
PATHOLOGY RESEARCH AND PRACTICE
(2023)
Article
Oncology
Yu Zheng, Jiansheng Wu, Hong Chen, Dajia Lin, Hongyuan Chen, Junyin Zheng, Haoyun Xia, Liangxiang Huang, Changqing Zeng
Summary: In this study, it was found that KLF4 enhances the sensitivity of colon cancer cells to 5-FU by targeting RAB26 to suppress the autophagy pathway.
CANCER BIOLOGY & THERAPY
(2023)
Article
Cell Biology
Guolin Zhang, Xin Luo, Zian Wang, Jianbin Xu, Wei Zhang, Engeng Chen, Qing Meng, Di Wang, Xuefeng Huang, Wei Zhou, Zhangfa Song
Summary: The study revealed that TIMP-2 is highly expressed in 5-Fu resistant colorectal cancer patients and its elevated levels contribute to drug resistance by activating the ERK/MAPK signaling pathway.
Article
Cell Biology
Sabrina Blondy, Hugo Talbot, Sofiane Saada, Niki Christou, Serge Battu, Julie Pannequin, Marie-Odile Jauberteau, Fabrice Lalloue, Mireille Verdier, Muriel Mathonnet, Aurelie Perraud
Summary: Colorectal cancer (CRC) is the third most common cancer worldwide, and resistance to 5-fluorouracil (5-FU) can lead to poor clinical outcomes. Sortilin overexpression has been identified as a potential biomarker of 5-FU resistance in CRC, with higher levels correlating with tumor grades. This new prognostic factor may be used to combat CRC aggressiveness.
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
(2021)
Article
Cell Biology
Christopher Minteer, Marco Morselli, Margarita Meer, Jian Cao, Albert Higgins-Chen, Sabine M. Lang, Matteo Pellegrini, Qin Yan, Morgan E. Levine
Summary: This study demonstrates that physiologically relevant aging changes can be induced in vitro and used to uncover mechanistic insights into epigenetic aging.
Article
Cell Biology
Meiling Zhang, Zongzhi Z. Liu, Keisuke Aoshima, Wesley L. Cai, Hongyin Sun, Tianrui Xu, Yangyi Zhang, Yongyan An, Jocelyn F. Chen, Lok Hei Chan, Asako Aoshima, Sabine M. Lang, Zhenwei Tang, Xuanlin Che, Yao Li, Sara J. Rutter, Veerle Bossuyt, Xiang Chen, Jon S. Morrow, Lajos Pusztai, David L. Rimm, Mingzhu Yin, Qin Yan
Summary: Transcriptomic analysis revealed that upregulation of CECR2 is associated with breast cancer metastasis and immune suppression. In mouse models, increased expression of CECR2 promotes breast cancer metastasis. Mechanistically, RELA, a member of the NF-kappa B family, recruits CECR2 to activate the expression of metastasis-related genes.
SCIENCE TRANSLATIONAL MEDICINE
(2022)
Article
Multidisciplinary Sciences
Farshad Farshidfar, Kahn Rhrissorrakrai, Chaya Levovitz, Cong Peng, James Knight, Antonella Bacchiocchi, Juan Su, Mingzhu Yin, Mario Sznol, Stephan Ariyan, James Clune, Kelly Olino, Laxmi Parida, Joerg Nikolaus, Meiling Zhang, Shuang Zhao, Yan Wang, Gang Huang, Miaojian Wan, Xianan Li, Jian Cao, Qin Yan, Xiang Chen, Aaron M. Newman, Ruth Halaban
Summary: Despite being the most common melanoma subtype among non-White individuals, the molecular drivers of acral melanoma remain unknown. In this study, the authors integrated genomic and clinical data from 104 patients and identified recurrent amplifications of cytoband 22q11.21 and LZTR1 as a key tumor promoter in this region.
NATURE COMMUNICATIONS
(2022)
Article
Biochemical Research Methods
Dingyao Zhang, Jingru Tian, Yadong Wang, Jun Lu
Summary: This study developed the Evitar pipeline for designing anti-viral siRNAs and CRISPR Cas13a gRNA sequences. Time simulations using known coronavirus genomes showed that pre-designed siRNAs and gRNAs could target SARS-CoV-2 and other viruses. In vitro experiments confirmed the potential of designed siRNAs to suppress SARS-CoV-2 viral sequences. The results support the utility of Evitar in pre-designing therapeutics against future RNA virus outbreaks.
Article
Immunology
Jingru Tian, Hang Zhou, Dorthy Fang, Ping Yi, Pengpeng Cao, Juan Liu, Feng Xiong, Bo Zhang, Yi Liu, George C. Tsokos, Qianjin Lu
Summary: This study aims to explore bias in lupus erythematosus randomized clinical trials (RCTs) and provide benchmarks for future trials. By systematically searching and conducting meta-analyses on global RCTs, it reveals the significant impact of national income and trial registration on trial results and participant characteristics, emphasizing the need for rigorously designed trials with diverse inclusion criteria to maintain repeatability and referenceability.
JOURNAL OF AUTOIMMUNITY
(2022)
Editorial Material
Multidisciplinary Sciences
Lok Hei Chan, Qin Yan
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Editorial Material
Oncology
Hongyin Sun, Bihui Huang, Jian Cao, Qin Yan, Mingzhu Yin
FRONTIERS IN ONCOLOGY
(2022)
Article
Rheumatology
Jingru Tian, Dingyao Zhang, Xu Yao, Yaqing Huang, Qianjin Lu
Summary: This study estimated the epidemiology of SLE globally, finding variations in incidence and prevalence rates across different countries and regions. It also highlighted the impact of gender, age, and prevalence estimation method on the findings. SLE was found to occur more frequently in high-income countries.
ANNALS OF THE RHEUMATIC DISEASES
(2023)
Article
Medicine, Research & Experimental
Hui Zheng, Lizhen Wu, Qian Xiao, Xin Meng, Alex Hafiz, Qin Yan, Renquan Lu, Jian Cao
Summary: DNA sensing through the cGAS-STING pathway is important in cancer immunosurveillance. Activation of STING in the tumor environment is an attractive approach to induce anti-tumor immunity, but it is limited due to epigenetic silencing of STING in many tumors. In this study, the inhibition of KDM5 family histone demethylases restored STING expression in breast cancer cells and activated the cGAS-STING pathway, showing the potential of KDM5 inhibitors as novel immune modulators in cancer therapies.
BIOMEDICINE & PHARMACOTHERAPY
(2023)
Review
Oncology
Goran Micevic, Marcus W. Bosenberg, Qin Yan
Summary: Immune checkpoint inhibitors (ICI) have shown significant improvements in treating various cancers, but there are still challenges to overcome. Some cold tumor types, like pancreatic cancer, have low response rates to ICI due to low immunogenicity. Additionally, patients who initially respond to ICI may experience T-cell exhaustion, leading to a lack of sustained response. Recent studies have highlighted the role of epigenetic modifiers in regulating tumor cell immunity and T-cell exhaustion, suggesting that targeting the intersection of epigenetics and immune checkpoint therapy could enhance antitumor immune responses.
CLINICAL CANCER RESEARCH
(2023)
Article
Genetics & Heredity
Xijuan Liu, Jun Wang, Joshua A. Boyer, Weida Gong, Shuai Zhao, Ling Xie, Qiong Wu, Cheng Zhang, Kanishk Jain, Yiran Guo, Javier Rodriguez, Mingjie Li, Hidetaka Uryu, Chengheng Liao, Lianxin Hu, Jin Zhou, Xiaobing Shi, Yi-Hsuan Tsai, Qin Yan, Weibo Luo, Xian Chen, Brian D. Strahl, Alex von Kriegsheim, Qi Zhang, Gang Greg Wang, Albert S. Baldwin, Qing Zhang
Summary: Histone H3 with hydroxylation of proline at residue 16 (H3P16oh) is an important histone post-translational modification (PTM) in mammalian cells, and it regulates mammalian gene expression.
Article
Immunology
Jingru Tian, Shuntong Kang, Dingyao Zhang, Yaqing Huang, Ming Zhao, Xianhua Gui, Xu Yao, Qianjin Lu
Summary: In this study, we estimated the unbiased epidemiological data of SSc globally and in specific countries through systematic searches of epidemiological studies. The results showed that 83.9% of countries worldwide lack epidemiological data on SSc. The global incidence and prevalence of SSc were estimated to be 8.64 per 100,000 person-years and 18.87 per 100,000 persons, respectively. There is a higher incidence and prevalence of SSc in females, adults, and high-income level countries. It is necessary to conduct large-scale and standardized epidemiological investigations of SSc, especially in low- and middle-income countries.
CLINICAL IMMUNOLOGY
(2023)
Article
Genetics & Heredity
Jin Wei, Ajinkya Patil, Clayton K. Collings, Mia Madel Alfajaro, Yu Liang, Wesley L. Cai, Madison S. Strine, Renata B. Filler, Peter C. DeWeirdt, Ruth E. Hanna, Bridget L. Menasche, Arya Okten, Mario A. Pena-Hernandez, Jon Klein, Andrew McNamara, Romel Rosales, Briana L. McGovern, M. Luis Rodriguez, Adolfo Garcia-Sastre, Kris M. White, Yiren Qin, John G. Doench, Qin Yan, Akiko Iwasaki, Thomas P. Zwaka, Jun Qi, Cigall Kadoch, Craig B. Wilen
Summary: This study demonstrates that the activity of the chromatin remodeling complex mSWI/SNF plays an important role in determining susceptibility to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection, highlighting it as a potential therapeutic target for SARS-CoV-2.
Article
Oncology
Gang Peng, Yibo Xi, Chiara Bellini, Kien Pham, Zhen W. Zhuang, Qin Yan, Man Jia, Guilin Wang, Lingeng Lu, Moon-Shong Tang, Hongyu Zhao, He Wang
Summary: Epigenomic-wide DNA methylation profiling has the potential to reflect risks associated with electronic cigarette exposure and individual poor health outcomes. Our study in male ApoE-/- mice reveals significant alterations in CpG sites after electronic cigarette exposure, with a high percentage annotated with known genes. Pathway analysis indicates activation of MAPK and cardiomyocyte-related signaling pathways, suggesting potential cell-damaging effects of prolonged e-cigarette inhalation.
AMERICAN JOURNAL OF CANCER RESEARCH
(2022)