PURE mRNA display and cDNA display provide rapid detection of core epitope motif via high‐throughput sequencing

Synthetic Biochemistry: The Bio-inspired Cell-Free Approach to Commodity Chemical Production

(2020) James U. Bowie et al.   TRENDS IN BIOTECHNOLOGY

In Vitro Selection of Peptides and Proteins—Advantages of mRNA Display

(2020) Matilda S. Newton et al.   ACS Synthetic Biology

A simple, robust, and low-cost method to produce the PURE cell-free system.

(2019) Barbora Lavickova et al.   ACS Synthetic Biology

Large-Scale, Quantitative Protein Assays on a High-Throughput DNA Sequencing Chip

(2019) Curtis J. Layton et al.   MOLECULAR CELL

A novel immuno-PCR method using cDNA display

(2019) Hiroki Anzai et al.   ANALYTICAL BIOCHEMISTRY

Synthetic Biology Goes Cell-Free

(2019) Aidan Tinafar et al.   BMC BIOLOGY

In vitro selection of anti-gliadin single-domain antibodies from a naïve library for cDNA-display mediated immuno-PCR

(2019) Chathuni Jayathilake et al.   ANALYTICAL BIOCHEMISTRY

A Bifunctional Polyphosphate Kinase Driving the Regeneration of Nucleoside Triphosphate and Reconstituted Cell-Free Protein Synthesis

(2019) Po-Hsiang Wang et al.   ACS Synthetic Biology

Basics of Antibody Phage Display Technology

(2018) Line Ledsgaard et al.   Toxins

RNA Display Methods for the Discovery of Bioactive Macrocycles

(2018) Yichao Huang et al.   CHEMICAL REVIEWS

Cell-free systems in the new age of synthetic biology

(2017) Fernando Villarreal et al.   Frontiers of Chemical Science and Engineering

High-Throughput Measurement of Binding Kinetics by mRNA Display and Next-Generation Sequencing

(2016) Farzad Jalali-Yazdi et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Cell-Free Synthetic Biology: Engineering Beyond the Cell

(2016) Jessica G. Perez et al.   Cold Spring Harbor Perspectives in Biology

A High Performance Platform Based on cDNA Display for Efficient Synthesis of Protein Fusions and Accelerated Directed Evolution

(2016) Mohammed Naimuddin et al.   ACS Combinatorial Science

Construction and screening of vast libraries of natural product-like macrocyclic peptides using in vitro display technologies

(2015) Nasir K Bashiruddin et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Conceptual and methodological advances in cell-free directed evolution

(2015) Igor Dodevski et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

PURE mRNA display forin vitroselection of single-chain antibodies

(2015) Yu Nagumo et al.   JOURNAL OF BIOCHEMISTRY

HeLa Based Cell Free Expression Systems for Expression of Plasmodium Rhoptry Proteins

(2015) Raghavendra Yadavalli et al.   Jove-Journal of Visualized Experiments

FASTAptamer: A Bioinformatic Toolkit for High-throughput Sequence Analysis of Combinatorial Selections

(2015) Khalid K Alam et al.   Molecular Therapy-Nucleic Acids

PURE ribosome display and its application in antibody technology

(2014) Takashi Kanamori et al.   BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS

Tobacco BY-2 cell-free lysate: an alternative and highly-productive plant-based in vitro translation system

(2014) Matthias Buntru et al.   BMC BIOTECHNOLOGY

Liposome display for in vitro selection and evolution of membrane proteins

(2014) Satoshi Fujii et al.   Nature Protocols

Identification of Amino Acid Residues Responsible for the Binding to Anti-FLAG™ M2 Antibody Using a Phage Display Combinatorial Peptide Library

(2013) Witsanu Srila et al.   APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY

mRNA display: from basic principles to macrocycle drug discovery

(2013) Kristopher Josephson et al.   DRUG DISCOVERY TODAY

In vitro evolution of  -hemolysin using a liposome display

(2013) S. Fujii et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Single-Round, Multiplexed Antibody Mimetic Design through mRNA Display

(2012) C. Anders Olson et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Synthetic biology and the development of tools for metabolic engineering

(2012) Jay D. Keasling   METABOLIC ENGINEERING

Next-generation sequencing coupled with a cell-free display technology for high-throughput production of reliable interactome data

(2012) Shigeo Fujimori et al.   Scientific Reports

Cell-free protein synthesis: Applications come of age

(2011) Erik D. Carlson et al.   BIOTECHNOLOGY ADVANCES

Cell-free synthetic biology: Thinking outside the cell

(2011) C. Eric Hodgman et al.   METABOLIC ENGINEERING

mRNA display for the selection and evolution of enzymes from in vitro-translated protein libraries

(2011) Burckhard Seelig   Nature Protocols

One-Pot Preparation of mRNA/cDNA Display by a Novel and Versatile Puromycin-Linker DNA

(2011) Yuki Mochizuki et al.   ACS Combinatorial Science

Directed evolution of a three-finger neurotoxin by using cDNA display yields antagonists as well as agonists of interleukin-6 receptor signaling

(2011) Mohammed Naimuddin et al.   Molecular Brain

Study of messenger RNA inactivation and protein degradation in an Escherichia coli cell-free expression system

(2010) Jonghyeon Shin et al.   Journal of Biological Engineering

Epitope Mapping Using Ribosome Display in a Reconstituted Cell-Free Protein Synthesis System

(2009) Eriko Osada et al.   JOURNAL OF BIOCHEMISTRY

cDNA display: a novel screening method for functional disulfide-rich peptides by solid-phase synthesis and stabilization of mRNA-protein fusions

(2009) J. Yamaguchi et al.   NUCLEIC ACIDS RESEARCH

Efficiency of cell-free protein synthesis based on a crude cell extract from Escherichia coli, wheat germ, and rabbit reticulocytes

(2007) Mami Hino et al.   JOURNAL OF BIOTECHNOLOGY