Principles and practice of determining metal–protein affinities

Calculating metalation in cells reveals CobW acquires CoII for vitamin B12 biosynthesis while related proteins prefer ZnII

(2021) Tessa R. Young et al.   Nature Communications

Bacterial sensors define intracellular free energies for correct enzyme metalation

(2019) Deenah Osman et al.   Nature Chemical Biology

The sub‐picomolar Cu2+ affinity of human serum albumin

(2019) Karolina Bossak-Ahmad et al.   CHEMBIOCHEM

Shift from Entropic Cu2+ Binding to Enthalpic Cu+ Binding Determines the Reduction Thermodynamics of Blue Copper Proteins

(2019) Molly L. North et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

The Scs disulfide reductase system cooperates with the metallochaperone CueP in Salmonella copper resistance

(2019) Pramod Subedi et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

The Human Amyloid Precursor Protein Binds Copper Ions Dominated by a Picomolar-Affinity Site in the Helix-Rich E2 Domain

(2018) Tessa R. Young et al.   BIOCHEMISTRY

Metal binding properties, stability and reactivity of zinc fingers

(2018) Katarzyna Kluska et al.   COORDINATION CHEMISTRY REVIEWS

Recent progress in the development of organic dye based near-infrared fluorescence probes for metal ions

(2018) Di Wu et al.   COORDINATION CHEMISTRY REVIEWS

Evaluation of Cu(i) binding to the E2 domain of the amyloid precursor protein – a lesson in quantification of metal binding to proteins via ligand competition

(2018) Tessa R. Young et al.   Metallomics

Ternary Zn(II) Complexes of FluoZin-3 and the Low Molecular Weight Component of the Exchangeable Cellular Zinc Pool

(2018) Ilona Marszałek et al.   INORGANIC CHEMISTRY

di-Cysteine motifs in the C-terminus of plant HMA4 proteins confer nanomolar affinity for zinc and are essential for HMA4 function in vivo

(2018) Gilles Lekeux et al.   JOURNAL OF EXPERIMENTAL BOTANY

Interaction of Cu(i) with the Met-X3-Met motif of alpha-synuclein: binding ligands, affinity and structural features

(2018) Iñaki Gentile et al.   Metallomics

Molecular Aspects of a Robust Assay for Ferroxidase Function of Ceruloplasmin

(2017) Laura Cortes et al.   INORGANIC CHEMISTRY

Molar absorption coefficients and stability constants of Zincon metal complexes for determination of metal ions and bioinorganic applications

(2017) Anna Kocyła et al.   JOURNAL OF INORGANIC BIOCHEMISTRY

High-affinity metal binding by the Escherichia coli [NiFe]-hydrogenase accessory protein HypB is selectively modulated by SlyD

(2017) Mozhgan Khorasani-Motlagh et al.   Metallomics

Copper binding and redox chemistry of the Aβ16 peptide and its variants: insights into determinants of copper-dependent reactivity

(2017) Nineveh Yako et al.   Metallomics

The zinc efflux activator SczA protects S treptococcus pneumoniae serotype 2 D39 from intracellular zinc toxicity

(2017) Julia E. Martin et al.   MOLECULAR MICROBIOLOGY

Fine control of metal concentrations is necessary for cells to discern zinc from cobalt

(2017) Deenah Osman et al.   Nature Communications

Analytical Methods for Imaging Metals in Biology: From Transition Metal Metabolism to Transition Metal Signaling

(2016) Cheri M. Ackerman et al.   ANALYTICAL CHEMISTRY

Dissecting ITC data of metal ions binding to ligands and proteins

(2016) Rachel A. Johnson et al.   BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS

Evaluation of employing poly-lysine tags versus poly-histidine tags for purification and characterization of recombinant copper-binding proteins

(2016) Chathuri J.K. Wijekoon et al.   JOURNAL OF INORGANIC BIOCHEMISTRY

Metal binding to the N-terminal cytoplasmic domain of the PIB ATPase HMA4 is required for metal transport in Arabidopsis

(2016) Clémentine Laurent et al.   PLANT MOLECULAR BIOLOGY

Calcium ions tune the zinc-sequestering properties and antimicrobial activity of human S100A12

(2016) Lisa S. Cunden et al.   Chemical Science

Unusual Zn(II) Affinities of Zinc Fingers of Poly(ADP-ribose) Polymerase 1 (PARP-1) Nuclear Protein

(2015) Karolina Bossak et al.   CHEMICAL RESEARCH IN TOXICOLOGY

CopC Protein from Pseudomonas fluorescens SBW25 Features a Conserved Novel High-Affinity Cu(II) Binding Site

(2015) Chathuri J. K. Wijekoon et al.   INORGANIC CHEMISTRY

Heavy Metals and Human Health: Mechanistic Insight into Toxicity and Counter Defense System of Antioxidants

(2015) Arif Jan et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Generating a Metal-responsive Transcriptional Regulator to Test What Confers Metal Sensing in Cells

(2015) Deenah Osman et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Molar absorption coefficients and stability constants of metal complexes of 4-(2-pyridylazo)resorcinol (PAR): Revisiting common chelating probe for the study of metalloproteins

(2015) Anna Kocyła et al.   JOURNAL OF INORGANIC BIOCHEMISTRY

A set of robust fluorescent peptide probes for quantification of Cu(ii) binding affinities in the micromolar to femtomolar range

(2015) Tessa R. Young et al.   Metallomics

The functional roles of the three copper sites associated with the methionine-rich insert in the multicopper oxidase CueO from E. coli

(2015) Laura Cortes et al.   Metallomics

Fluorescent Sensors for Measuring Metal Ions in Living Systems

(2014) Kyle P. Carter et al.   CHEMICAL REVIEWS

Redox sulfur chemistry of the copper chaperone Atox1 is regulated by the enzyme glutaredoxin 1, the reduction potential of the glutathione couple GSSG/2GSH and the availability of Cu(i)

(2014) Jens Brose et al.   Metallomics

Metal binding properties of the zinc finger metallome – insights into variations in stability

(2014) Anna Miłoch et al.   Metallomics

An integrated study of the affinities of the Aβ16 peptide for Cu(i) and Cu(ii): implications for the catalytic production of reactive oxygen species

(2014) Tessa R. Young et al.   Metallomics

Robust Affinity Standards for Cu(I) Biochemistry

(2013) Pritha Bagchi et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Evaluation of quantitative probes for weaker Cu(i) binding sites completes a set of four capable of detecting Cu(i) affinities from nanomolar to attomolar

(2013) Zhiguang Xiao et al.   Metallomics

Ternary complex formation and competition quench fluorescence of ZnAF family zinc sensors

(2013) Anna Staszewska et al.   Metallomics

Co(ii)-detection does not follow Kco(ii) gradient: channelling in Co(ii)-sensing

(2013) Carl J. Patterson et al.   Metallomics

Quantification of copper binding to amyloid precursor protein domain 2 and its Caenorhabditis elegans ortholog. Implications for biological function

(2013) Su Ling Leong et al.   Metallomics

Cu(II) Affinity for the Alzheimer’s Peptide: Tyrosine Fluorescence Studies Revisited

(2012) Bruno Alies et al.   ANALYTICAL CHEMISTRY

Affinity of copper and zinc ions to proteins and peptides related to neurodegenerative conditions (Aβ, APP, α-synuclein, PrP)

(2012) Izabela Zawisza et al.   COORDINATION CHEMISTRY REVIEWS

Calcium Ion Gradients Modulate the Zinc Affinity and Antibacterial Activity of Human Calprotectin

(2012) Megan Brunjes Brophy et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Ultracentrifugation and inductively coupled plasma mass spectrometry for metal–protein equilibrium studies

(2012) Isaac J. Arnquist et al.   SPECTROCHIMICA ACTA PART B-ATOMIC SPECTROSCOPY

Mycobacterium tuberculosisNmtR Harbors a Nickel Sensing Site with Parallels toEscherichia coliRcnR

(2011) Hermes Reyes-Caballero et al.   BIOCHEMISTRY

Copper-Binding Properties and Structures of Methanobactins from Methylosinus trichosporium OB3b

(2011) Abdelnasser El Ghazouani et al.   INORGANIC CHEMISTRY

Unification of the Copper(I) Binding Affinities of the Metallo-chaperones Atx1, Atox1, and Related Proteins

(2011) Zhiguang Xiao et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

A Water-Soluble Bis(thiosemicarbazone) Ligand. A Sensitive Probe and Metal Buffer for Zinc

(2010) Gojko Buncic et al.   INORGANIC CHEMISTRY

Application of isothermal titration calorimetry in bioinorganic chemistry

(2010) Nicholas E. Grossoehme et al.   JOURNAL OF BIOLOGICAL INORGANIC CHEMISTRY

The Metalloregulatory Zinc Site in Streptococcus pneumoniae AdcR, a Zinc-activated MarR Family Repressor

(2010) Hermes Reyes-Caballero et al.   JOURNAL OF MOLECULAR BIOLOGY

Reaction Mechanisms of the Multicopper Oxidase CueO fromEscherichia coliSupport Its Functional Role as a Cuprous Oxidase

(2010) Karrera Y. Djoko et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

The challenges of determining metal–protein affinities

(2010) Zhiguang Xiao et al.   NATURAL PRODUCT REPORTS

Metal Binding Affinities ofArabidopsisZinc and Copper Transporters: Selectivities Match the Relative, but Not the Absolute, Affinities of their Amino-Terminal Domains,

(2009) Matthias Zimmermann et al.   BIOCHEMISTRY

Unprecedented Binding Cooperativity between CuIand CuIIin the Copper Resistance Protein CopK fromCupriavidus metalliduransCH34: Implications from Structural Studies by NMR Spectroscopy and X-Ray Crystallography

(2009) Lee Xin Chong et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Metalloproteins and metal sensing

(2009) Kevin J. Waldron et al.   NATURE

The interaction of biological and noxious transition metals with the zinc probes FluoZin-3 and Newport Green

(2008) Jinfu Zhao et al.   ANALYTICAL BIOCHEMISTRY

Bioinorganic chemistry of copper and zinc ions coordinated to amyloid-β peptide

(2008) Peter Faller et al.   DALTON TRANSACTIONS

Transfer of Copper between Bis(thiosemicarbazone) Ligands and Intracellular Copper-Binding Proteins. Insights into Mechanisms of Copper Uptake and Hypoxia Selectivity

(2008) Zhiguang Xiao et al.   INORGANIC CHEMISTRY

Metal ions in biological catalysis: from enzyme databases to general principles

(2008) Claudia Andreini et al.   JOURNAL OF BIOLOGICAL INORGANIC CHEMISTRY

Ni(II) and Co(II) Sensing by Escherichia coli RcnR

(2008) Jeffrey S. Iwig et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Isothermal titration calorimetry of metal ions binding to proteins: An overview of recent studies

(2007) Dean E. Wilcox   INORGANICA CHIMICA ACTA