Review
Immunology
Shobhit Gairola, Antarip Sinha, Ravinder K. Kaundal
Summary: Pulmonary fibrosis is a devastating disorder characterized by inflammation and matrix accumulation. Activation of NLRP3 is directly correlated with the development of pulmonary fibrosis. IL-1β, by affecting the activity of various transcription factors including TGF-β, promotes myofibroblast activation and lung fibrosis.
INFLAMMOPHARMACOLOGY
(2023)
Article
Immunology
Yanbing Qiu, Yumei Huang, Meilin Chen, Yuqin Yang, Xiaoxu Li, Wenling Zhang
Summary: NLRP3 inflammasome is an intracellular polyprotein complex that is activated by perceiving different molecular patterns. Intracellular mtDNA and extracellular mtDNA have different functions in activating NLRP3 inflammasome.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Yeon-Ji Park, Niranjan Dodantenna, Yonghyeon Kim, Tae-Hwan Kim, Ho-Soo Lee, Young-Suk Yoo, June Heo, Jae-Ho Lee, Myung-Hee Kwon, Ho Chul Kang, Jong-Soo Lee, Hyeseong Cho
Summary: The NLRP3 inflammasome is activated through mitochondria-mediated ubiquitination by the E3 ligase MARCH5. Knockout of MARCH5 leads to an impaired secretion of IL-1β and IL-18 in myeloid cells, as well as a decreased mortality rate upon infection. MARCH5-dependent NLRP3 ubiquitination is essential for NLRP3-NEK7 complex formation and NLRP3 oligomerization.
Article
Environmental Sciences
Bingwei Yang, Yuting Wang, Changyu Fang, Erqun Song, Yang Song
Summary: Exposure to PBDEs metabolite PBDEQ induces ROS and mitochondrial dysfunction, promoting NLRP3 inflammasome activation through the TLRs-NF-kappa B signaling pathway. Additionally, bromine metabolism and K+ efflux are involved in the activation of this inflammatory response.
ENVIRONMENTAL POLLUTION
(2022)
Article
Biochemistry & Molecular Biology
Ilandarage Menu Neelaka Molagoda, Athapaththu Mudiyanselage Gihan Kavinda Athapaththu, Yung Hyun Choi, Cheol Park, Cheng-Yung Jin, Chang-Hee Kang, Mi-Hwa Lee, Gi-Young Kim
Summary: The study demonstrates that Fisetin inhibits the activation of the NLRP3 inflammasome by TLR4/MD2 pathway, leading to the elimination of damaged mitochondria through p62-dependent mitophagy.
Article
Biochemistry & Molecular Biology
Grace A. Ward, Robert P. P. Dalton III, Benjamin S. Meyer, Amy F. McLemore, Amy L. Aldrich, Nghi B. Lam, Alexis H. Onimus, Nicole D. Vincelette, Thu Le Trinh, Xianghong Chen, Alexandra R. Calescibetta, Sean M. Christiansen, Hsin-An Hou, Joseph O. Johnson, Kenneth L. Wright, Eric Padron, Erika A. Eksioglu, Alan F. List
Summary: Myelodysplastic Syndromes (MDSs) are characterized by innate immune activation and pyroptotic cell death driven by NLRP3 inflammasome. The release of oxidized mitochondrial DNA (ox-mtDNA) into the cytosol enhances the inflammatory response in healthy tissues via engagement with Toll-like receptor 9 (TLR9). Blocking the TLR9/ox-mtDNA axis may be a promising therapeutic strategy for MDS.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Multidisciplinary Sciences
Hong Dong, Bao Zhao, Jianwen Chen, Zihao Liu, Xinghui Li, Lupeng Li, Haitao Wen
Summary: Mitochondria play a crucial role in regulating innate immune function by controlling the repair of phagolysosomal membranes and promoting NLRP3 inflammasome activation. This mechanism links mitochondrial metabolism, immune cell function, and the development of inflammatory responses.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Review
Biochemistry & Molecular Biology
Tan Zhang, Shuzhe Ding, Ru Wang
Summary: NLRP3 is a crucial pattern recognition receptor in the innate immune system, with mitochondria playing a key role in inflammasome activation. Exercise is considered an important intervention to regulate immune responses, with different types of training impacting NLRP3 inflammasome activation differently.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Qingqing Ke, Caifeng Shi, Yunhui Lv, Lulu Wang, Jing Luo, Lei Jiang, Junwei Yang, Yang Zhou
Summary: This study analyzed the anti-fibrotic and anti-inflammatory effects of the SGLT2 inhibitor dapagliflozin on renal alternations. It was found that dapagliflozin mitigated inflammation and accumulation of metabolites in fibrosis renal tissue through the modulation of metabolism and immune-responsive genes. The study also demonstrated that dapagliflozin blocked the activation of the NLRP3 inflammasome, protecting the kidney from fibrosis development. This research identifies a novel mechanism coupling metabolism and inflammation for the kidney benefits of SGLT2 inhibition.
Article
Immunology
Yingting Hou, Hongbin He, Ming Ma, Rongbin Zhou
Summary: NLRP3 is an important innate immune sensor that activates the inflammasome complex, resulting in the secretion of IL-1 beta and pyroptosis. The mechanism of NLRP3 inflammasome activation in response to crystals or particulates is unclear, but lysosomal damage has been implicated. This study discovered that apilimod, a lysosomal disruptor, is a selective and potent NLRP3 agonist that triggers mitochondrial damage and lysosomal dysfunction, leading to NLRP3 inflammasome activation.
FRONTIERS IN IMMUNOLOGY
(2023)
Review
Pharmacology & Pharmacy
Pablo Pelegrin
Summary: ATP plays an essential role in cellular energetics and signaling. Extracellular ATP in inflammatory environments activates immune cells through P2X7 receptor, leading to the formation of inflammasomes and inflammatory cell death. Research on novel anti-inflammatory therapies targeting P2X7 receptor and NLRP3 pathway is ongoing.
BIOCHEMICAL PHARMACOLOGY
(2021)
Article
Plant Sciences
Wei Shi, Tingting Liu, Huijie Yang, Jia Zhao, Ziying Wei, Yujiao Huang, Zhiyong Li, Hui Li, Longxin Liang, Xiaorong Hou, Yuanyuan Chen, Yuan Gao, Zhaofang Bai, Xiaohe Xiao
Summary: DC can induce idiosyncratic HILI through the activation of NLRP3 inflammasome. This study aims to investigate the mechanism of DC-induced idiosyncratic HILI and identify the susceptible component.
JOURNAL OF ETHNOPHARMACOLOGY
(2024)
Article
Biochemistry & Molecular Biology
Mi Nam Lee, Youngyoon Lee, Dayong Wu, Munkyong Pae
Summary: The NLRP3 inflammasome is an important component in the pathogenesis of various diseases, and luteolin may potentially suppress NLRP3 activation to combat inflammatory diseases.
JOURNAL OF NUTRITIONAL BIOCHEMISTRY
(2021)
Article
Immunology
Hu Yue, Zhongjin Yang, Yitao Ou, Shuli Liang, Wenmin Deng, Hao Chen, Cheng Zhang, Lei Hua, Wenhui Hu, Ping Sun
Summary: Tanshinones, derived from the roots of Salvia miltiorrhiza, are active ingredients widely used in traditional medicine for treating inflammatory diseases. This study identified 15 tanshinones that suppress the activation of NLRP3 inflammasome, with specific tanshinones showing potential mitochondrial protection via promoting autophagy and the AMP-activated protein kinase pathway. The findings suggest that tanshinones, particularly dihydrotanshinone I, may offer a pharmacological mechanism for effectively treating inflammatory diseases like septic and gouty inflammation.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2021)
Article
Medicine, Research & Experimental
Ching-Chun Yang, Chih-Hsing Wu, Ta-Chun Lin, Yi-Ning Cheng, Chin-Sung Chang, Kuo-Ting Lee, Pei-Jane Tsai, Yau-Sheng Tsai
Summary: The study reveals an additional anti-inflammatory role for PPAR gamma in suppressing NLRP3 inflammasome activation through interaction with NLRP3.
Article
Medicine, Research & Experimental
A. Lama, F. Del Piano, C. Annunziata, F. Comella, N. Opallo, S. Melini, L. Grumetto, C. Pirozzi, G. Mattace Raso, R. Meli, M. C. Ferrante
Summary: In this study, it was found that bisphenol A (BPA) exacerbates anxiety-like behavior induced by high-fat diet (HFD) in obese mice, and also increases neuroinflammation and immune activation. The results suggest that BPA represents an additional risk factor for mood disorders related to obesity, by enhancing HFD-induced neuroinflammation and immune activation.
Article
Biochemistry & Molecular Biology
Gina Cavaliere, Angela Catapano, Giovanna Trinchese, Fabiano Cimmino, Eduardo Penna, Amelia Pizzella, Claudia Cristiano, Adriano Lama, Marianna Crispino, Maria Pina Mollica
Summary: Neurodegenerative diseases (NDDs) are characterized by cognitive impairment and behavioural abnormalities. High-fat diets have been shown to cause mitochondrial dysfunction and affect synaptic plasticity, linking obesity and NDDs. Butyrate, a short-chain fatty acid, has been found to improve mitochondrial function and reduce neuroinflammation and oxidative stress in a mouse model of diet-induced obesity.
Article
Biochemistry & Molecular Biology
Giuseppina Chianese, Annalisa Lopatriello, Carmina Sirignano, Alessandro Andreani, Andrea Gambini, Eric De Combarieu, Mariano Stornaiuolo, Orazio Taglialatela-Scafati
Summary: Two unprecedented cyclopamine analogues, 18-hydroxycyclopamine and 24R-hydroxycyclopamine, with modifications on rings D-F, were isolated from the mother liquors obtained from crystallization of cyclopamine extracted from corn lily. The stereostructures of these new natural compounds were determined through detailed MS and 1D/2D NMR investigation. Their inhibition of the hedgehog pathway was evaluated using the dual-luciferase bioassay, providing new insights into the structure-activity relationships for this class of compounds.
BIOORGANIC & MEDICINAL CHEMISTRY
(2023)
Article
Pharmacology & Pharmacy
Serena Sagliocchi, Melania Murolo, Annunziata Gaetana Cicatiello, Caterina Miro, Annarita Nappi, Emery Di Cicco, Sepehr Torabinejad, Evelina La Civita, Veronica Romano, Daniela Terracciano, Mariano Stornaiuolo, Monica Dentice
Summary: The iodothyronine deiodinases are a group of enzymes that regulate thyroid hormone metabolism and affect various physiological processes. Deregulation of these enzymes is associated with several human pathologies. This study identifies Cefuroxime as a novel inhibitor of D2 activity, potentially offering a new treatment option for hyperthyroid pathologies.
PHARMACOLOGICAL RESEARCH
(2023)
Article
Environmental Sciences
Filomena Del Piano, Adriano Lama, Giovanni Piccolo, Nicola Francesco Addeo, Doriana Iaccarino, Giovanna Fusco, Lorenzo Riccio, Davide De Biase, Giuseppina Mattace Raso, Rosaria Meli, Maria Carmela Ferrante
Summary: This study aimed to assess the effects of microplastics ingestion on gut innate immunity and barrier integrity in gilthead seabreams. The results showed that ingesting polystyrene microplastics can cause inflammation and immune alterations, as well as disrupt intestinal epithelial integrity.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Food Science & Technology
Francesco Errichiello, Maria 'Amato, Angelita Gambuti, Luigi Moio, Arianna Pastore, Hekmat AL-Hmadi, Mariano Stornaiuolo, Elena Serino, Orazio Taglialatela-Scafati, Martino Forino
Summary: Grape pomace, a component of winery waste, contains various healthy compounds with bioactivities. In addition to polyphenols, an in-depth chemical analysis of Aglianico grape pomace revealed a remarkable concentration of oleanolic acid, a natural triterpenoid with multiple bioactivities. The study also assessed the influence of oleanolic acid on mitochondrial and glucose uptake activities, supporting its potential as an antidiabetic metabolite.
JOURNAL OF FUNCTIONAL FOODS
(2023)
Article
Multidisciplinary Sciences
Annarita Nappi, Caterina Miro, Antonio Pezone, Alfonso Tramontano, Emery Di Cicco, Serena Sagliocchi, Annunziata Gaetana Cicatiello, Melania Murolo, Sepehr Torabinejad, Elena Abbotto, Giuseppina Caiazzo, Maddalena Raia, Mariano Stornaiuolo, Dario Antonini, Gabriella Fabbrocini, Domenico Salvatore, Vittorio Enrico Avvedimento, Monica Dentice
Summary: In this study, the authors investigate the role of thyroid hormones in tumor progression and identify the thyroid hormone activating enzyme D2 as a p53 target gene. They find that p53, as a cell stress sensor and tumor suppressor, suppresses D2 expression, leading to decreased availability of intracellular thyroid hormones. Conversely, loss of p53 enhances D2/TH signaling, promoting the fitness of tumor cells through modulation of genes involved in DNA damage and repair and redox signaling. In vivo deletion of D2 significantly reduces cancer progression, suggesting that targeting thyroid hormones could be a general approach to reduce invasiveness in p53-mutated neoplasms.
NATURE COMMUNICATIONS
(2023)
Review
Oncology
Alessandro Ottaiano, Mariachiara Santorsola, Luisa Circelli, Anna Maria Trotta, Francesco Izzo, Francesco Perri, Marco Cascella, Francesco Sabbatino, Vincenza Granata, Marco Correra, Luca Tarotto, Salvatore Stilo, Francesco Fiore, Nicola Martucci, Antonello La Rocca, Carmine Picone, Paolo Muto, Valentina Borzillo, Andrea Belli, Renato Patrone, Edoardo Mercadante, Fabiana Tatangelo, Gerardo Ferrara, Annabella Di Mauro, Giosue Scognamiglio, Massimiliano Berretta, Maurizio Capuozzo, Angela Lombardi, Jerome Galon, Oreste Gualillo, Ugo Pace, Paolo Delrio, Giovanni Savarese, Stefania Scala, Guglielmo Nasti, Michele Caraglia
Summary: Oligometastatic disease is a condition in which cancer spreads to only a few distant sites, and it is associated with a more favorable prognosis compared to polymetastatic disease. Recent studies have identified specific molecular and genetic features that underlie the oligometastatic phenotype, suggesting the potential for personalized therapies. This scoping review highlights new clinical, biological, and methodological challenges in understanding and treating oligometastatic disease.
Article
Pharmacology & Pharmacy
Giovanni Smaldone, Elisabetta Rosa, Enrico Gallo, Carlo Diaferia, Giancarlo Morelli, Mariano Stornaiuolo, Antonella Accardo
Summary: Hydrogel nanoparticles, also called nanogels, can be used as supramolecular vehicles for delivering biologically relevant molecules. Understanding the intracellular mechanism of nanogel uptake by cancer cells can contribute to their potential diagnostic and clinical applications.
Article
Agriculture, Dairy & Animal Science
Filomena Del Piano, Adriano Lama, Anna Monnolo, Claudio Pirozzi, Giovanni Piccolo, Simone Vozzo, Davide De Biase, Lorenzo Riccio, Giovanna Fusco, Raffaelina Mercogliano, Rosaria Meli, Maria Carmela Ferrante
Summary: Plastics are a major pollutant in the sea, breaking down into microplastic particles that can cause toxic effects in aquatic organisms. This study focused on the gilthead seabream and found that ingestion of microplastics increases oxidative and nitrosative stress in the intestine, impairing the antioxidant defense system. This damage to the gut can lead to health issues in fish.
Article
Biochemistry & Molecular Biology
Elisabetta Schiano, Enrico Cappello, Domenico Cecere, Francesco Pompeo, Ettore Novellino, Mariano Stornaiuolo, Marcello Izzo
Summary: Under pathological conditions, the level of Non-Transferrin Bound Iron (NTBI) in the blood increases, leading to oxidative stress and damage to organs and tissues. This study focuses on measuring and analyzing the NTBI pool in the serum of peripheral arterial disease (PAD) patients. The researchers found that NTBI in PAD patients is predominantly bound to the serum protein Albumin, and developed a simple immunoisolation procedure to quantify NTBI-Albumin complexes for clinical screening purposes.
Article
Microbiology
Carla Zannella, Annalisa Chianese, Giuseppe Annunziata, Annalisa Ambrosino, Anna De Filippis, Gian Carlo Tenore, Ettore Novellino, Mariano Stornaiuolo, Massimiliano Galdiero
Summary: Herpes simplex virus (HSV) is a common virus that causes oral or genital ulcers and can lead to severe complications. Current anti-HSV drugs have limitations, so there is a need for new antiviral compounds. This study tested a polyphenol-based nutraceutical formulation called Taurisolo(R), which showed potential in inhibiting the early stages of HSV-1 and HSV-2 infection. These findings suggest that Taurisolo(R) could be used as a topical treatment for preventing and healing herpes lesions.
Article
Biochemistry & Molecular Biology
Aurore Fraix, Cristina Parisi, Giuseppe Longobardi, Claudia Conte, Arianna Pastore, Mariano Stornaiuolo, Adriana C. E. Graziano, Marta E. E. Alberto, Antonio Frances-Monerris, Fabiana Quaglia, Salvatore Sortino
Summary: Activation of nitric oxide (NO) release in the red-light range using a green-light excitation method has been demonstrated. This method allows for precise control of NO release without the need for chemical modification or sophisticated irradiation sources. The effectiveness of this strategy has been confirmed in a biological environment, making it a potential candidate for nanotherapeutics.
Article
Biochemistry & Molecular Biology
Caterina Miro, Annarita Nappi, Serena Sagliocchi, Emery Di Cicco, Melania Murolo, Sepehr Torabinejad, Lucia Acampora, Arianna Pastore, Paolo Luciano, Evelina La Civita, Daniela Terracciano, Mariano Stornaiuolo, Monica Dentice, Annunziata Gaetana Cicatiello
Summary: Thyroid hormones play a role in regulating muscle formation and energy utilization by modulating carbohydrates, lipids, and proteins. They can change the lipid composition and fatty acid ratio in skeletal muscle, with increased proportion of unsaturated fatty acids improving insulin sensitivity and endurance exercise.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Meeting Abstract
Gastroenterology & Hepatology
Stefania Melini, Claudio Pirozzi, Adriano Lama, Filomena Del Piano, Federica Comella, Nicola Opallo, Chiara Annunziata, Giuseppina Mattace Raso, Rosaria Meli
JOURNAL OF HEPATOLOGY
(2023)