Article
Biochemistry & Molecular Biology
Yong Gao, Yanan Zhang, Chen Feng, Honglong Chu, Chao Feng, Haibo Wang, Lifang Wu, Si Yin, Chao Liu, Huanhuan Chen, Zhumei Li, Zhengrong Zou, Lizhou Tang
Summary: In this study, a chromosome-level genome assembly of Amorphophallus konjac was generated, revealing the mechanisms underlying the large genome size and the key genes involved in glucomannan biosynthesis. The expansion of transposable elements was found to contribute to the large genome size, and valuable candidate genes for molecular breeding of this crop were identified.
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
(2022)
Article
Food Science & Technology
Fernanda Laignier, Rita de Cassia de Almeida Akutsu, Bernardo Romao de Lima, Renata Puppin Zandonadi, Antonio Raposo, Ariana Saraiva, Raquel Braz Assuncao Botelho
Summary: This study evaluated the sensory profile of gluten-free bread with different concentrations of Amorphophallus konjac (AK) flour. The results showed that the addition of 12.5% AK flour resulted in gluten-free bread with sensory characteristics closer to regular bread.
Article
Biochemistry & Molecular Biology
Yujie Su, Mianzhang Zhang, Cuihua Chang, Junhua Li, Yuanyuan Sun, Yundan Cai, Wen Xiong, Luping Gu, Yanjung Yang
Summary: The Amorphophallus bulbifer (A. bulbifer) has the potential to be used as KGM resources in the food industry due to its good growth characteristics and high KGM content. However, the high viscosity of KGM from A. bulbifer and the browning of KGM gels limit its application. Citric-acid treatment (CAT) significantly reduces the viscosity and ΔE value of KGM, making it valuable for developing low-calorie foods without affecting gel texture properties.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Chemistry, Applied
Meng Wang, Yun-Cheng Li, Fan-Bing Meng, Qiao Wang, Zheng-Wu Wang, Da-Yu Liu
Summary: In this study, honeysuckle leaf extract was added to the CMKH composite film. The addition of honeysuckle leaf extract slightly decreased the water vapor barrier properties of the film, but completely blocked UV transmission. The film with honeysuckle leaf extract showed higher elongation at break and exhibited strong antioxidant and antimicrobial activities.
Article
Food Science & Technology
Shishuai Wang, Lidong Ding, Shuo Chen, Ying Zhang, Jiaxin He, Bin Li
Summary: This study investigated the effect of konjac glucomannan (KGM) on the retrogradation of amylose. The results showed that with increasing amount of KGM, the color of the system became darker, and the elastic modulus, viscous modulus, and tangent value of loss angle increased. The textural result showed that KGM significantly inhibited the growth rate of gel strength of amylose. WHC and XRD results indicated that as the concentration of KGM increased, WHC increased and crystallinity degree declined. LF-NMR results revealed that KGM limited the conversion of free water to bound water. Overall, this research suggests that a small amount of KGM can inhibit the short-term and long-term retrogradation of amylose.
Article
Plant Sciences
Hao Cui, Xinying Zhu, Zhaoguang Wang, Jianping Fang, Tao Yuan
Summary: The structurally defined konjac glucomannan oligosaccharide (KGMOS) with specific molecular sizes showed beneficial effects on colonic functions in mice by regulating intestinal microflora composition to facilitate butyrate production, enhancing histone acetylation, and promoting mucin secretion, thus improving gut health through a new molecular mechanism.
JOURNAL OF NATURAL PRODUCTS
(2021)
Article
Biochemistry & Molecular Biology
Yan Chen, Xuefeng Gao, Bin Li, Jing Tian
Summary: The study investigated the effects of the interaction between konjac glucomannan (KGM) and dihydromyricetin (DMY) on KGM's viscosity, hydration capacity, and DMY's thermal stability. Contrary to most reports, adding DMY to KGM increased viscosity and hydration capacity and improved DMY's retention and radical scavenging capacity under heating conditions. The KGM-DMY complex was characterized as a non-covalent compound connected by hydrogen bonds. This study is beneficial for the development of polyphenol-enriched nutrition based on KGM, particularly in terms of satiety, appetite regulation, and glucose regulation.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Food Science & Technology
Fernanda Laignier, Rita de Cassia Coelho de Almeida Akutsu, Iriani Rodrigues Maldonade, Maria Teresa Bertoldo Pacheco, Vera Sonia Nunes Silva, Marcio Antonio Mendonca, Renata Puppin Zandonadi, Antonio Raposo, Raquel Braz Assuncao Botelho
Summary: The study found that adding different concentrations of konjac flour can improve the specific volume of gluten-free bread, reduce its calorie content, and increase fiber content. The best formulation was found with a konjac flour concentration of up to 37.5%, while the bread with 50% konjac flour showed slightly reduced specific volume and a pale color.
Article
Agriculture, Multidisciplinary
Ziqiang Duan, Yuntao Wang, Xiao Yu, Nan Wu, Jie Pang, Yanhong Bai
Summary: In this study, the effects of konjac oligo-glucomannan (KOG) on the emulsifying properties of myofibrillar protein (MP) were investigated. It was found that the addition of KOG altered the tertiary conformation of MP, resulting in improved emulsifying activity, decreased particle size, and improved physical stability of the emulsion. The findings suggest that KOG interacts with MP at the oil-water interface, forming a stable interface film to enhance the emulsifying properties of MP.
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
(2023)
Article
Biochemistry & Molecular Biology
Ana Carolina Santos Fernandes, Luana Muxfeldt, Neli Gehlen Motta, Calinca Skonieski, Karina Raquel Fagundes, Gabriela Sandri, Daniel Barbosa de Chaves, Gabriela Suthovski, Andre Lazarin Gallina, Stifani Machado Araujo, Dalila Moter Benvegnu
Summary: This study evaluated the effects of consuming gummy candies enriched with Konjac glucomannan (KGM) on appetite, anthropometric data, and biochemical markers in overweight individuals. The results showed that participants who consumed KGM for 14 days experienced a reduction in waist circumference and intensity of hunger/satisfaction. Longer consumption and higher doses of KGM are believed to contribute to even more satisfactory body results.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Biochemistry & Molecular Biology
Zhenyu Li, Ling Zhang, Chunling Mao, Zhiming Song, Xinxin Li, Chun Liu
Summary: This study evaluated composite gels prepared with konjac glucomannan (KGM) and gum arabic (GA) using various analytical methods, revealing gel stratification at certain concentrations, changes in microstructure with GA concentration, and increases in water solubility and water absorption with higher GA concentrations. Rheological analysis showed the gels to be non-Newtonian pseudoplastic fluids with different deformation characteristics.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Chemistry, Multidisciplinary
Marta Redondo, Rafaela Presa, Pedro L. Granja, Marco Araujo, Aureliana Sousa
Summary: This study successfully developed degradable Konjac glucomannan (KGM) hydrogels for 3D cell culture and achieved cell-material interactions by modifying and grafting bioactive peptides into KGM. The results show great potential for the further application of KGM in tissue engineering.
MATERIALS TODAY CHEMISTRY
(2023)
Article
Agriculture, Multidisciplinary
Di Wu, Simin Yu, Hongshan Liang, Mohamed Eid, Bin Li, Jing Li, Jin Mao
Summary: The study found that the addition of appropriate amounts of MD and DKGM can alter the tensile properties of KGM/CAR mixed gel, potentially meeting consumer needs and allowing for the design of innovative products in the soft gel industry.
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
(2021)
Article
Chemistry, Applied
Dongling Qiao, Jieyi Lu, Wenjuan Shi, Hao Li, Liang Zhang, Fatang Jiang, Binjia Zhang
Summary: Deacetylation enhances chain interactions, reduces crystallite formation, improves hydrophobicity and mechanical performance, and increases matrix flexibility and elongation at break in KGM-based composite films.
CARBOHYDRATE POLYMERS
(2022)
Article
Chemistry, Applied
Xinli Ran, Hongshun Yang
Summary: This research investigated the effect of konjac glucomannan (KGM) concentrations on the molecular interactions, gelation behaviors, and viscoelastic properties of a soy protein - konjac glucomannan complex gel. The results showed that increasing KGM concentration enhanced the strain-hardening effect and promoted protein denaturation and aggregation. The gel strength and crystalline index of the complex gel improved significantly with increased KGM incorporation. This study provides fundamental support for the potential application of the KGM-protein complex in plant-based seafood alternatives.
FOOD HYDROCOLLOIDS
(2022)
Article
Radiology, Nuclear Medicine & Medical Imaging
Yuan Zhang, Mei Wang, Xiaotong Zhang, Zhenyi Jiang, Yuanxiang Zhang, Xiangjun Fu, Yanna Li, Danping Cao, Jun Han, Jiucui Tong
Summary: DNA methylation is associated with stress responses and depression. Antidepressants can regulate DNA methylation and gene expression. The study found that SH2D5 expression is low in depression, but can be overexpressed by helicid treatment. Furthermore, helicid may improve apoptosis indices in C6 cells by regulating the DNA methylation of SH2D5.
CONTRAST MEDIA & MOLECULAR IMAGING
(2022)
Article
Biophysics
Lei Hu, Jin Pan, Cuifeng Zhang, Kun Yu, Shuting Shen, Yaxuan Wang, Xuebin Shen, Xiaoxia Gu, Jun Han, Hui Wang
Summary: In this study, a red emission fluorophore probe LD-HW with high fluorescence quantum yield and selective recognition of lipid droplet polarity variation was successfully constructed. It could rapidly light up lipid droplets and monitor their dynamic behavior in real-time through a wash-free procedure. The probe showed great potential in non-invasive diagnosis of fatty liver.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Immunology
Yuyan Zhou, Ruoxuan Xiang, Guozheng Qin, Baozhen Ji, Shuo Yang, Guodong Wang, Jun Han
Summary: This study found that the xanthone-rich fraction of Securidaca inappendiculata can reduce the severity of collagen-induced arthritis in rats, regulate the polarization of macrophages, and achieve joint protection by inhibiting the NAMPT/glycolysis pathway.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Xiu-Yun Yin, Huan-Xin Chen, Zhuo Chen, Qin Yang, Jun Han, Guo-Wei He
Summary: Four variants in the promoter region of the ISL1 gene were identified in ASD patients only, with three of them significantly decreasing transcriptional activities. These variants may affect binding sites of transcription factors, potentially influencing the development of ASD.
JOURNAL OF GENE MEDICINE
(2022)
Article
Immunology
Yuyan Zhou, Lina Liu, Ruoxuan Xiang, Xiaoyang Bu, Guozheng Qin, Jiajia Dai, Zhigang Zhao, Xue Fang, Shuo Yang, Jun Han, Guodong Wang
Summary: In this study, it was found that Arctigenin (AR) extracted from Burdock has therapeutic effects on type 2 diabetes by reducing blood glucose and lipid levels, reversing tissue damage, and inhibiting inflammation. The mechanism of action seems to involve the inhibition of toll-like receptor 4 inflammatory signaling and reactivation of the IRS-2/GLUT4 pathway.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2023)
Article
Cell Biology
Tian-Tian Jiang, Cong-Lan Ji, Li-Jun Yu, Meng-Ke Song, Yan Li, Qiang Liao, Tuo Wei, Opeyemi Joshua Olatunji, Jian Zuo, Jun Han
Summary: This study investigated the effects of SIRT1 activation on RA-related angiogenesis. The results showed that glycolysis was accelerated in RA serum-treated HUVECs, leading to ATP accumulation. SIRT1 activation inhibited pro-angiogenesis cytokines production, glycolysis, and angiogenesis capabilities in both HUVECs and rat vascular epithelial cells.
INFLAMMATION RESEARCH
(2023)
Article
Chemistry, Applied
Wen Cao, Jiangping Wu, Xinya Zhao, Zixu Li, Jie Yu, Taili Shao, Xuefeng Hou, Lutan Zhou, Chunfei Wang, Guodong Wang, Jun Han
Summary: In this study, a water-soluble polysaccharide (PLP1) was successfully isolated and purified from Pueraria lobata. It was found that PLP1 was composed of specific glycosidic units and exhibited a better free radical-scavenging ability. Moreover, PLP1 effectively protected the liver against acute alcoholic liver disease (ALD) in mice.
CARBOHYDRATE POLYMERS
(2024)
Article
Chemistry, Applied
Zhiyan Tang, Mingrui Zhang, Lan Gao, Yulong Bao, Ping Li, Ming Wang, Taili Shao, Guodong Wang, Chunyan Liu
Summary: This study optimized the extraction conditions of a polysaccharide from Stevia rebaudiana roots and demonstrated its ability to counter oxidative stress, improve cell viability, increase antioxidant enzyme activity, and reduce oxidative damage. These findings highlight the potential of SRRP as a natural antioxidant in the food and pharmaceutical industries.
NATURAL PRODUCT RESEARCH
(2023)
Article
Veterinary Sciences
Chunyan Liu, Pingchuan Yuan, Ying Wang, Xuelian Yang, Yudie Xu, Wenzhi Zhang, Xue Han, Kaoshan Chen, Guodong Wang, Taili Shao
Summary: Long-term consumption of burdock inulin-type fructans (ITFs) has no obvious adverse effects on the health of parental mice and their offspring, but may contribute to reproductive capacity, fatigue reduction, and risk reduction of renal disease.
JOURNAL OF VETERINARY MEDICAL SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Zhiyan Tang, Taili Shao, Lan Gao, Pingchuan Yuan, Zhengrui Ren, Lei Tian, Wei Liu, Chunyan Liu, Xiuxian Xu, Xuan Zhou, Jun Han, Guodong Wang
Summary: Diabetes mellitus is a common chronic condition characterized by high blood sugar levels due to abnormal insulin functionality, with type 2 diabetes being the most prevalent form. A fructan called SRRP was extracted from Stevia rebaudiana roots and purified, and it was found to have a molecular weight of 5.4 x 10(3) Da. In vivo experiments showed that SRRP significantly lowered fasting blood glucose levels, improved insulin resistance, reduced oxidative stress, and regulated lipid metabolism in type 2 diabetes mouse models. Furthermore, SRRP altered the gut microbiota and its metabolites in these mice, increasing beneficial bacteria and short-chain fatty acid concentration while decreasing harmful bacteria. These findings suggest the potential of SRRP in treating type 2 diabetes.
Article
Endocrinology & Metabolism
J. I. N. G. Zhang, L. I. N. A. Liu, P. I. N. G. C. H. U. A. N. Yuan, X. I. U. X. I. A. N. Xu, S. H. U. A. I. W. E. N. Chang, X. U. E. Fang, G. U. O. Z. H. E. N. G. Qin, G. U. O. D. O. N. G. Wang, Y. U. Y. A. N. Zhou
Summary: TUDCA protects renal proximal tubular cells against apoptosis induced by high glucose by suppressing endoplasmic reticulum (ER) stress. This protective effect may be associated with the inhibition of reactive oxygen species (ROS) and stabilization of mitochondrial membrane potential.
JOURNAL OF BIOLOGICAL REGULATORS AND HOMEOSTATIC AGENTS
(2022)
Article
Biochemistry & Molecular Biology
Wei Liu, Taili Shao, Lei Tian, Zhengrui Ren, Lan Gao, Zhiyan Tang, Zheng Fang, Pingchuan Yuan, Chunyan Liu, Jikun Li, Guodong Wang, Jun Han
Summary: In this study, a homogeneous polysaccharide PCP1 was extracted and purified from Polygonatum cyrtonema Hua. The chemical structure analysis showed that PCP1 is mainly composed of fructose, glucose, and mannose. In vivo experiments demonstrated that PCP1 has potential therapeutic effects on non-alcoholic fatty liver disease (NAFLD) by mitigating liver pathological damage, improving abnormal lipid metabolism, promoting the production of short-chain fatty acids, and balancing the composition of the intestinal microbiota.
Article
Cardiac & Cardiovascular Systems
Zhuo Chen, Huan-Xin Chen, Hai-Tao Hou, Xiu-Yun Yin, Qin Yang, Jun Han, Guo-Wei He
Summary: Variants in the promoter region of CITED2 gene affect its transcriptional activity in ASD patients, suggesting their involvement in the occurrence and development of ASD.
JOURNAL OF CARDIOVASCULAR DEVELOPMENT AND DISEASE
(2022)
