Inactivation of N-Acetylglucosaminyltransferase I and α1,3-Fucosyltransferase Genes in Nicotiana tabacum BY-2 Cells Results in Glycoproteins With Highly Homogeneous, High-Mannose N-Glycans

Glycosylation as a tool for rational vaccine design

(2020) Vivek Hariharan et al.   BIOTECHNOLOGY AND BIOENGINEERING

Evolutionary and Predictive Functional Insights into the Aquaporin Gene Family in the Allotetraploid Plant Nicotiana tabacum

(2020) Jahed Ahmed et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Golgi localization of GnTI requires a polar amino acid residue within its transmembrane domain

(2019) Jennifer Schoberer et al.   PLANT PHYSIOLOGY

Exploring the N-Glycosylation Profile of Glycoprotein B from Human Cytomegalovirus Expressed in CHO and Nicotiana tabacum BY-2 Cells

(2019) Nicolas Smargiasso et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Production of recombinant human acid β-glucosidase with high mannose-type N-glycans in rice gnt1 mutant for potential treatment of Gaucher disease

(2019) Jae-Wan Jung et al.   PROTEIN EXPRESSION AND PURIFICATION

N-glycosylation regulates photosynthetic efficiency of Arabidopsis thaliana

(2019) Q.-S. JIAO et al.   PHOTOSYNTHETICA

CRISPR/Cas9-mediated knockout of six glycosyltransferase genes in Nicotiana benthamiana for the production of recombinant proteins lacking β-1,2-xylose and core α-1,3-fucose

(2018) Julia Jansing et al.   PLANT BIOTECHNOLOGY JOURNAL

A plant cell-expressed recombinant anti-TNF fusion protein is biologically active in the gut and alleviates immune-mediated hepatitis and colitis

(2017) Yaron Ilan et al.   IMMUNOBIOLOGY

Revisiting the substrate specificity of mammalian α1,6-fucosyltransferase reveals that it catalyzes core fucosylation ofN-glycans lacking α1,3-arm GlcNAc

(2017) Qiang Yang et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Production of recombinant human acid α-glucosidase with high-mannose glycans in gnt1 rice for the treatment of Pompe disease

(2017) Jae-Wan Jung et al.   JOURNAL OF BIOTECHNOLOGY

Establishment of a tobacco BY2 cell line devoid of plant-specific xylose and fucose as a platform for the production of biotherapeutic proteins

(2017) Uri Hanania et al.   PLANT BIOTECHNOLOGY JOURNAL

N -glycan maturation mutants in Lotus japonicus for basic and applied glycoprotein research

(2017) Carina T. Pedersen et al.   PLANT JOURNAL

N-glycan structures and downstream mannose-phosphorylation of plant recombinant human alpha-l-iduronidase: toward development of enzyme replacement therapy for mucopolysaccharidosis I

(2017) Owen M. Pierce et al.   PLANT MOLECULAR BIOLOGY

Inactivation of the β(1,2)-xylosyltransferase and the α(1,3)-fucosyltransferase genes in Nicotiana tabacum BY-2 Cells by a Multiplex CRISPR/Cas9 Strategy Results in Glycoproteins without Plant-Specific Glycans

(2017) Sébastien Mercx et al.   Frontiers in Plant Science

Plant protein glycosylation

(2016) Richard Strasser   GLYCOBIOLOGY

Substrate specificity of FUT8 and chemoenzymatic synthesis of core-fucosylated asymmetric N-glycans

(2016) Angie D. Calderon et al.   ORGANIC & BIOMOLECULAR CHEMISTRY

The production of human glucocerebrosidase in glyco-engineeredNicotiana benthamianaplants

(2016) Juthamard Limkul et al.   PLANT BIOTECHNOLOGY JOURNAL

Putting the Spotlight Back on Plant Suspension Cultures

(2016) Rita B. Santos et al.   Frontiers in Plant Science

Using GlycoDelete to produce proteins lacking plant-specific N-glycan modification in seeds

(2015) Robin Piron et al.   NATURE BIOTECHNOLOGY

Large-scale production of pharmaceutical proteins in plant cell culture-the protalix experience

(2015) Yoram Tekoah et al.   PLANT BIOTECHNOLOGY JOURNAL

The combination of plant translational enhancers and terminator increase the expression of human glucocerebrosidase in Nicotiana benthamiana plants

(2015) Juthamard Limkul et al.   PLANT SCIENCE

Boosting CRISPR/Cas9 multiplex editing capability with the endogenous tRNA-processing system

(2015) Kabin Xie et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Cas-OFFinder: a fast and versatile algorithm that searches for potential off-target sites of Cas9 RNA-guided endonucleases

(2014) S. Bae et al.   BIOINFORMATICS

GlycoDelete engineering of mammalian cells simplifies N-glycosylation of recombinant proteins

(2014) Leander Meuris et al.   NATURE BIOTECHNOLOGY

The transmembrane domain ofN -acetylglucosaminyltransferase I is the key determinant for its Golgi subcompartmentation

(2014) Jennifer Schoberer et al.   PLANT JOURNAL

Multiplex and homologous recombination–mediated genome editing in Arabidopsis and Nicotiana benthamiana using guide RNA and Cas9

(2013) Jian-Feng Li et al.   NATURE BIOTECHNOLOGY

Accumulation of secreted antibodies in plant cell cultures varies according to the isotype, host species and culture conditions

(2013) Bertrand Magy et al.   PLANT BIOTECHNOLOGY JOURNAL

Optimization of BY-2 cell suspension culture medium for the production of a human antibody using a combination of fractional factorial designs and the response surface method

(2013) Nikolay Vasilev et al.   PLANT BIOTECHNOLOGY JOURNAL

Characterization and downstream mannose phosphorylation of human recombinant α-L-iduronidase produced in Arabidopsiscomplex glycan-deficient(cgl) seeds

(2013) Xu He et al.   PLANT BIOTECHNOLOGY JOURNAL

A plant culture (BY-2) widely used in molecular and cell studies is genetically unstable and highly heterogeneous

(2012) Ales Kovarik et al.   BOTANICAL JOURNAL OF THE LINNEAN SOCIETY

Identification, gene cloning and expression of serine proteases in the extracellular medium of Nicotiana tabacum cells

(2012) Catherine Navarre et al.   PLANT CELL REPORTS

N-glycan maturation is crucial for cytokinin-mediated development and cellulose synthesis inOryza sativa

(2012) Wahyu Indra Duwi Fanata et al.   PLANT JOURNAL

Production of active human glucocerebrosidase in seeds of Arabidopsis thaliana complex-glycan-deficient (cgl) plants

(2011) Xu He et al.   GLYCOBIOLOGY

Reduced Immunogenicity ofArabidopsis hgl1MutantN-Glycans Caused by Altered Accessibility of Xylose andcoreFucose Epitopes

(2011) Heidi Kaulfürst-Soboll et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

High-Mannose Glycan-Dependent Epitopes Are Frequently Targeted in Broad Neutralizing Antibody Responses during Human Immunodeficiency Virus Type 1 Infection

(2011) C. L. Lavine et al.   JOURNAL OF VIROLOGY

Glycosylation of Therapeutic Proteins

(2010) Ricardo J. Solá et al.   BIODRUGS

Optimal nitrogen supply as a key to increased and sustained production of a monoclonal full-size antibody in BY-2 suspension culture

(2010) T. Holland et al.   BIOTECHNOLOGY AND BIOENGINEERING

Plant-specific glycosylation patterns in the context of therapeutic protein production

(2010) Véronique Gomord et al.   PLANT BIOTECHNOLOGY JOURNAL

New Strategies for Enzyme Replacement Therapy for Lysosomal Storage Diseases

(2010) Jeffrey H. Grubb et al.   REJUVENATION RESEARCH

Change in glycosylation pattern with extension of endoplasmic reticulum retention signal sequence of mouse antibody produced by suspension-cultured tobacco BY2 cells

(2009) Kazuhito Fujiyama et al.   JOURNAL OF BIOSCIENCE AND BIOENGINEERING

Laser desorption ionization of red phosphorus clusters and their use for mass calibration in time-of-flight mass spectrometry

(2009) Kateřina Sládková et al.   RAPID COMMUNICATIONS IN MASS SPECTROMETRY

Different subcellular localization and glycosylation for a functional antibody expressed in Nicotiana tabacum plants and suspension cells

(2009) Benoit De Muynck et al.   TRANSGENIC RESEARCH

Comparative Analyses of Arabidopsis complex glycan1 Mutants and Genetic Interaction with staurosporin and temperature sensitive3a

(2008) J. Frank et al.   PLANT PHYSIOLOGY

Salt tolerance of Arabidopsis thaliana requires maturation of N-glycosylated proteins in the Golgi apparatus

(2008) J. S. Kang et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA