Article
Clinical Neurology
Laura Pasetto, Maurizio Grassano, Silvia Pozzi, Silvia Luotti, Eliana Sammali, Alice Migazzi, Manuela Basso, Giovanni Spagnolli, Emiliano Biasini, Edoardo Micotti, Milica Cerovic, Mirjana Carli, Gianluigi Forloni, Giovanni De Marco, Umberto Manera, Cristina Moglia, Gabriele Mora, Bryan J. Traynor, Adriano Chio, Andrea Calvo, Valentina Bonetto
Summary: Deficiency of PPIA leads to a neurodegenerative disease with features resembling frontotemporal dementia in mice, characterized by mislocalization and aggregation of Ran protein in the brain, affecting the expression levels of TDP-43 and proteins involved in synaptic function. These findings suggest that defective PPIA function causes TDP-43 mislocalization and dysfunction, implicating it as a potential target for future therapeutic approaches.
Article
Plant Sciences
Jiacai Chen, Xinying Sui, Binran Ma, Yuetong Li, Na Li, Longfei Qiao, Yanchong Yu, Chun-Hai Dong
Summary: This study elucidates the regulatory mechanism of Arabidopsis CPR5 in ethylene signaling, including its interaction with the ETR1 receptor and its control over the nucleocytoplasmic transport of ethylene-related mRNAs. These findings have significant implications for our understanding of the role of CPR5 in ethylene signaling.
PLANT CELL REPORTS
(2022)
Article
Oncology
Giulio Sartori, Sara Napoli, Luciano Cascione, Elaine Yee Lin Chung, Valdemar Priebe, Alberto Jesus Arribas, Afua Adjeiwaa Mensah, Michela Dall'Angelo, Chiara Falzarano, Laura Barnabei, Mattia Forcato, Andrea Rinaldi, Silvio Bicciato, Margot Thome, Francesco Bertoni
Summary: FLI1 is more highly expressed in GCB DLBCL compared to ABC DLBCL and regulates a network of biologically crucial genes. It directly influences the NF-kappa B pathway at the transcriptional level and the alternative NF-kappa B pathway via ASB2, providing a potential new therapeutic approach for GCB DLBCL.
JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH
(2021)
Article
Cell Biology
Ruizhi Li, Kristin F. Wilson, Richard A. Cerione
Summary: ATF4 is a key stress-responsive transcription factor that aids cancer cells in surviving oxidative stress, nutrient deprivation, and matrix detachment. The mTORC2-PKC-Nrf2 pathway and Sirt5 are crucial for sustaining ATF4 response.
CELL DEATH DISCOVERY
(2022)
Article
Hematology
Alexia Katsarou, Nikolaos Trasanidis, Kanagaraju Ponnusamy, Ioannis V. Kostopoulos, Jaime Alvarez-Benayas, Foteini Papaleonidopoulou, Keren Keren, Pierangela M. R. Sabbattini, Niklas Feldhahn, Maria Papaioannou, Evdoxia Hatjiharissi, Ian M. Sudbery, Aristeidis Chaidos, Valentina S. Caputo, Anastasios Karadimitris
Summary: This study reveals that oncogenic MAF acts as a pioneer transcription factor in multiple myeloma, initiating and sustaining oncogenic transcriptomes and cancer phenotypes. Ectopic expression of MAF activates enhancers and superenhancers, leading to the conversion of transcriptionally inert chromatin to active chromatin with superenhancer features. The cooperation of MAF with other transcription factors is also crucial for the activation of oncogenic transcriptional programs. Despite its pioneer function, myeloma cells remain MAF-dependent, validating oncogenic MAF as a therapeutic target to overcome genetic diversification-driven disease relapse and drug resistance.
Article
Multidisciplinary Sciences
Diana Saleiro, Jeremy Q. Wen, Ewa M. Kosciuczuk, Frank Eckerdt, Elspeth M. Beauchamp, Chidera Oku, Gavin T. Blyth, Mariafausta Fischietti, Liliana Ilut, Marco Colamonici, William Palivos, Paula A. Atsaves, Dean Tan, Masha Kocherginsky, Rona Singer Weinberg, Eleanor N. Fish, John D. Crispino, Ronald Hoffman, Leonidas C. Platanias
Summary: Interferon alpha (IFNalpha) therapy is showing promising results in the treatment of myeloproliferative neoplasms (MPNs). This study reveals that the response to IFNalpha requires ULK1 phosphorylation to activate p38-MAPK signaling, but it is hindered by ROCK1-2 activation. The combination therapy of IFNalpha with ROCK1-2 inhibition may enhance the treatment of MPNs.
NATURE COMMUNICATIONS
(2022)
Article
Biochemistry & Molecular Biology
Joana Pinto Couto, Milica Vulin, Charly Jehanno, Marie-May Coissieux, Baptiste Hamelin, Alexander Schmidt, Robert Ivanek, Atul Sethi, Konstantin Brautigam, Anja L. Frei, Carolina Hager, Madhuri Manivannan, Jorge Gomez-Miragaya, Milan M. S. Obradovic, Zsuzsanna Varga, Viktor H. Koelzer, Kirsten D. Mertz, Mohamed Bentires-Alj
Summary: The NNMT gene in breast cancer is associated with metastasis and enhances tumor cell plasticity, and is associated with poor clinical prognosis. Removal of NNMT can inhibit metastasis formation in mouse models. In addition, removal of NNMT also increases DNA methylation of PRDM5 and extracellular matrix-related genes, thereby reducing the transcription level of the metastasis-related gene COL1A1 and inhibiting lung metastasis of breast cancer.
Article
Oncology
Colin Hutton, Felix Heider, Adrian Blanco-Gomez, Antonia Banyard, Alexander Kononov, Xiaohong Zhang, Saadia Karim, Viola Paulus-Hock, Dale Watt, Nina Steele, Samantha Kemp, Elizabeth K. J. Hogg, Joanna Kelly, Rene-Filip Jackstadt, Filipa Lopes, Matteo Menotti, Luke Chisholm, Angela Lamarca, Juan Valle, Owen J. Sansom, Caroline Springer, Angeliki Malliri, Richard Marais, Marina Pasca di Magliano, Santiago Zelenay, Jennifer P. Morton, Claus Jorgensen
Summary: This study used mass cytometry to analyze the stromal composition in murine tissues and tumors, revealing extensive stromal heterogeneity and coordinated relationships between mesenchymal and immune cell subsets in pancreatic ductal adenocarcinoma. The research identified two stable and functionally distinct pancreatic fibroblast lineages marked by CD105 expression, with CD105-positive fibroblasts promoting tumor growth and CD105-negative fibroblasts suppressing tumors, dependent on adaptive immunity. These findings highlight the importance of mesenchymal and immune cell interactions in restricting tumor growth.
Article
Multidisciplinary Sciences
Sonia Feu, Fernando Unzueta, Amaia Ercilla, Alejandro Perez-Venteo, Montserrat Jaumot, Neus Agell
Summary: Solving the problems encountered by replication forks during DNA synthesis is crucial for preventing genomic instability. RAD51 plays a prominent role in the S phase, not only in DNA repair during the G2 phase, but also in nascent DNA protection and replication fork restart. RAD51 inhibition does not significantly affect replication fork progression under non-perturbed conditions, but becomes necessary to maintain replication fork progression under replication stress. In colorectal cancer cells, the inhibition of RAD51 slows down replication fork speed and increases gamma H2Ax foci, indicating the presence of basal replication stress.
Article
Multidisciplinary Sciences
Stephanie Solier, Sebastian Mueller, Tatiana Caneque, Antoine Versini, Arnaud Mansart, Fabien Sindikubwabo, Leeroy Baron, Laila Emam, Pierre Gestraud, G. Dan Pantos, Vincent Gandon, Christine Gaillet, Ting-Di Wu, Florent Dingli, Damarys Loew, Sylvain Baulande, Sylvere Durand, Valentin Sencio, Cyril Robil, Francois Trottein, David Pericat, Emmanuelle Naeser, Celine Cougoule, Etienne Meunier, Anne-Laure Begue, Helene Salmon, Nicolas Manel, Alain Puisieux, Sarah Watson, Mark A. Dawson, Nicolas Servant, Guido Kroemer, Djillali Annane, Raphael Rodriguez
Summary: Inflammation is a complex physiological process that involves immune cells clearing sources of injury. Excessive inflammation is associated with infection and several diseases. The cell surface glycoprotein CD44 mediates the uptake of metals, including copper, and affects the metabolic and epigenetic states involved in inflammation.
Article
Biochemistry & Molecular Biology
Hiromitsu Kunimi, Deokho Lee, Mari Ibuki, Yusaku Katada, Kazuno Negishi, Kazuo Tsubota, Toshihide Kurihara
Summary: In this study, using retina-specific Hif-1 alpha conditional knockout (cKO) mice revealed that the reduction of Hif-1 alpha can lead to a decrease in inner retinal neurodegeneration. Experiments showed that depletion of Bnip3 in retinal neurons can have neuroprotective effects, supporting the critical role of the HIF-1 alpha/BNIP3 pathway in inner retinal neurodegeneration.
Article
Multidisciplinary Sciences
Wen-Liang Zhou, Kristen Kim, Farhan Ali, Steven T. Pittenger, Cali A. Calarco, Yann S. Mineur, Charu Ramakrishnan, Karl Deisseroth, Alex C. Kwan, Marina R. Picciotto
Summary: Dopamine signaling from the ventral tegmental area (VTA) is important for reward-related behaviors. This study reveals that neighboring VTA GABAergic neurons project to the ventral pallidum (VP) and their activity correlates with reward size and palatability. Stimulation of this GABA projection enhances the activity of VP neurons involved in seeking reward and improves performance in a cue-reward task.
Article
Immunology
Jonathan B. Johnnidis, Yuki Muroyama, Shin Foong Ngiow, Zeyu Chen, Sasikanth Manne, Zhangying Cai, Shufei Song, Jesse M. Platt, Jason M. Schenkel, Mohamed Abdel-Hakeem, Jean-Christophe Beltra, Allison R. Greenplate, Mohammed-Alkhatim A. Ali, Kito Nzingha, Josephine R. Giles, Christelle Harly, John Attanasio, Kristen E. Pauken, Bertram Bengsch, Michael A. Paley, Vesselin T. Tomov, Makoto Kurachi, Dario A. A. Vignali, Arlene H. Sharpe, Steven L. Reiner, Avinash Bhandoola, F. Bradley Johnson, E. John Wherry
Summary: The study identifies a distinct subset of memory T cells during the expansion phase of acute viral infection, which possess unique characteristics and play an important role in CD8(+) T cell memory. This subset, although quantitatively minor in the TCF-1(+) pool, exhibits self-renewal and long-term recall capacity.
SCIENCE IMMUNOLOGY
(2021)
Review
Infectious Diseases
Medha Tandon, Saurabh Kataria, Jenil Patel, Tejas R. Mehta, Maha Daimee, Viral Patel, Apoorv Prasad, Anisa Anila Chowdhary, Shruti Jaiswal, Shitiz Sriwastava
Summary: Limited literature exists on Cerebrospinal fluid (CSF) findings in COVID-19 patients with neurological symptoms. Analysis of 113 patients showed elevated CSF protein levels in fatal cases and higher CSF protein and cell counts in severe cases. Further studies on the pathophysiology of neurological complications in COVID-19 are recommended.
INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES
(2021)
Article
Biology
Michael Elbaz, Amalia Callado Perez, Maxime Demers, Shengli Zhao, Conrad Foo, David Kleinfeld, Martin Deschenes
Summary: Vibrissa sensory inputs play a crucial role in rodent behavior, with lemniscal and paralemniscal pathways mediating these inputs. While lemniscal projections are mapped from brainstem to cortex, the extent and topography of paralemniscal projections are unclear. This study demonstrates that paralemniscal projections transmit sensory signals to brainstem regions regulating autonomic functions and forebrain regions involved in emotional reactions, with GABAergic cells in the Kolliker-Fuse nucleus modulating trigeminal sensory input in the paralemniscal pathway.
Editorial Material
Cell Biology
Hyun Jung Hwang, Yoon Ki Kim
Summary: LC3B plays a crucial role in autophagy by binding to RNA and facilitating the degradation of mRNA.
Article
Engineering, Biomedical
Heejeong Yoon, Jeong Kon Seo, Tae-Eun Park
Summary: A new in vitro tool called adipose tissue microphysiological system (MPS) has been developed to mimic the pathophysiology of adipose tissue in obesity, providing valuable insights into the development of obesity-related disorders.
ACTA BIOMATERIALIA
(2023)
Article
Engineering, Biomedical
Jeong-Won Choi, Jaeseung Youn, Dong Sung Kim, Tae-Eun Park
Summary: Researchers have developed a blood-brain barrier (BBB) model based on an ultra-thin engineered basement membrane (nEBM) that reproduces the physical barrier function and efflux pump activity of the in vivo human BBB. This model can mimic ischemic stroke and provide valuable insights into the dynamic changes of the BBB, immune cell infiltration, and drug response, making it a powerful tool for drug permeation prediction and therapeutic strategy development for brain diseases.
Article
Genetics & Heredity
Hoyeon Lee, Chunghun Lim
Summary: This study demonstrates that circadian clocks regulate light-induced arousal in Drosophila sleep behavior. The arousal effects are mediated by glutamate transmission from octopaminergic neurons and are masked by light. Different groups of circadian pacemaker neurons are involved in light-induced arousal and free-running behavioral rhythms. These findings provide convincing evidence for circadian control of sleep.
JOURNAL OF NEUROGENETICS
(2023)
Article
Chemistry, Multidisciplinary
Jeong-Won Choi, Minwook Seo, Kyunghwan Kim, A-Ru Kim, Hakmin Lee, Hyung-Seok Kim, Chun Gwon Park, Seung Woo Cho, Joo H. Kang, Jinmyoung Joo, Tae-Eun Park
Summary: By using a human microphysiological system (MPS)-based SELEX method, a human BBB shuttle aptamer (hBS) with high efficiency in crossing the blood-brain barrier was successfully screened. The hBS demonstrated enhanced uptake efficiency in brain microvascular endothelial cells and brain cells, and showed high specificity and accumulation in the brain.
Article
Chemistry, Multidisciplinary
Su Hyun Jung, Bong Hwan Jang, Seyong Kwon, Sung Jin Park, Tae-Eun Park, Joo H. Kang
Summary: Autologous implantable scaffolds using autologous whole blood are effective in promoting skin wound healing by constructing robust microcapillary vessel networks. Microfluidic shear stresses enable the alignment of bundled fibrin fibers and the activation of platelets, facilitating endothelial cell maturation and vascularization. Rodent dorsal skin wounds patched with IVET exhibit superior wound closure rates, epidermis thickness, collagen deposition, hair follicle numbers, and neutrophil infiltration, attributed to enhanced microvascular circulation. Moreover, IVET treatment accelerates wound healing by recruiting M2 phenotype macrophages.
ADVANCED MATERIALS
(2023)
Article
Biophysics
Jonathan Sabate del Rio, Jooyoung Ro, Heejeong Yoon, Tae-Eun Park, Yoon-Kyoung Cho
Summary: Organs-on-chips (OoCs) are biomimetic in vitro systems that mimic the physicochemical microenvironments, physiologies, and key functional units of specific human organs using microfluidic cell cultures. These systems have the potential to replace animal models, enable personalized medicine, and require continuous monitoring of quality parameters. Integration of biosensing technologies into OoCs allows for monitoring of their physiologies, functions, and microenvironments. Future directions involve the application of artificial intelligence for process optimization, self-regulation, and data analysis in OoCs and cyber-physical systems.
BIOSENSORS & BIOELECTRONICS
(2023)
Review
Cell & Tissue Engineering
Jung In Park, Seung Woo Cho, Joo H. Kang, Tae-Eun Park
Summary: In this review, the unique structure and function of intestinal Peyer's patches (PPs) are summarized, along with the current technologies to establish in vitro PP models. It is found that PPs are surrounded by follicle-associated epithelium containing microfold (M) cells, which serve as special gateways for luminal antigen transport across the gut epithelium. However, the current in vitro PP models are not sufficient to recapitulate the function of PPs, and more advanced three-dimensional cell culture technologies are needed.
TISSUE ENGINEERING AND REGENERATIVE MEDICINE
(2023)
Article
Biochemistry & Molecular Biology
Jeeyoon Chang, Min-Kyung Shin, Joori Park, Hyun Jung Hwang, Nicolas Locker, Junhak Ahn, Doyeon Kim, Daehyun Baek, Yeonkyoung Park, Yujin Lee, Sung Ho Boo, Hyeong-In Kim, Yoon Ki Kim
Summary: The direct interaction between eIF4A3 and eIF3g plays a key role in the internal translation initiation of circRNAs, expanding our understanding of the protein-coding potential of the human transcriptome.
NUCLEIC ACIDS RESEARCH
(2023)
Article
Multidisciplinary Sciences
Hyun Jung Hwang, Tae Lim Park, Hyeong-In Kim, Yeonkyoung Park, Geunhee Kim, Chiyeol Song, Won-Ki Cho, Yoon Ki Kim
Summary: This study reveals the m(6)A-independent role of YTHDF2 in the formation of cytoplasmic protein aggregates known as aggresomes. YTHDF2 interacts with a complex associated with misfolded polypeptides and facilitates their movement towards aggresomes through the interaction between the dynein motor protein and the complex. These findings demonstrate that YTHDF2 is involved in protein quality control.
NATURE COMMUNICATIONS
(2023)
Article
Chemistry, Multidisciplinary
Hye-Jin Jeong, Ji-Hyeon Park, Joo H. H. Kang, Jonathan Sabate del Rio, Seong-Ho Kong, Tae-Eun Park
Summary: A human stomach micro-physiological system (hsMPS) combining organoid and MPS technology is described. The system enables the functional maturation of gastric epithelial cells and the recreation of a mesh-like mucus layer and well-developed epithelial junctional complexes. It successfully demonstrates gastroprotection mechanisms against Helicobacter pylori.
Article
Biochemistry & Molecular Biology
Dongkeun Park, Youngim Yu, Ji-hyung Kim, Jongbin Lee, Jongmin Park, Kido Hong, Jeong-Kon Seo, Chunghun Lim, Kyung-Tai Min
Summary: Thermal stress induces dynamic changes in nuclear proteins and relevant physiology as part of the heat shock response (HSR). Mitochondrial activity plays an important role in nuclear proteostasis and genome stability through two distinct HSR pathways. Suboptimal mitochondrial activity sustains nuclear homeostasis under cellular stress, providing evidence for optimal endosymbiotic evolution via mitochondria-to-nuclear communication.
MOLECULES AND CELLS
(2023)
Review
Biochemistry & Molecular Biology
Byungju Kim, Jincheol Seol, Yoon Ki Kim, Jong-Bong Lee
Summary: Single-molecule fluorescence imaging allows for the observation of circular mRNA formation and the intricate interplay between messenger RNA and ribosomal proteins during translation. This detailed visualization method could potentially enable direct manipulation of interactions within mRNA-protein complexes.
EXPERIMENTAL AND MOLECULAR MEDICINE
(2023)
Article
Biochemistry & Molecular Biology
Mi-Yeon Kim, Mi Jeong Kim, Changyeob Lee, Juwon Lee, Sang Seong Kim, Sungho Hong, Hyoung Tae Kim, Jinsoo Seo, Ki-Jun Yoon, Sungho Han
Summary: Trametinib as a MEK1/2 inhibitor promotes adult neurogenesis in an Alzheimer's disease mouse model and human iPSC-derived neural stem cells, suggesting its potential as a therapeutic approach for AD and other neurodegenerative diseases.
EXPERIMENTAL AND MOLECULAR MEDICINE
(2023)
