Article
Agricultural Engineering
Yantao Liu, Yanan Wang, Mingliang Tang, Man Zhao, Xiaobing Yang, Lingling Li, Yi Liu, Wenting Zhou, Zhiwei Gong
Summary: A simple combination utilization of water hyacinth hydrolysate and crude glycerol was developed to achieve lipid overproduction. The optimization of carbon-to-nitrogen ratio (C/N) and the dilution detoxification improved the quality of the lipids produced.
INDUSTRIAL CROPS AND PRODUCTS
(2022)
Article
Biotechnology & Applied Microbiology
Mikolaj Chmielarz, Johanna Blomqvist, Sabine Sampels, Mats Sandgren, Volkmar Passoth
Summary: This study tested the ability of oleaginous yeasts to utilize crude glycerol and hemicellulose hydrolysate as carbon sources, finding that only a few strains were able to convert them into microbial lipids. Mixing crude glycerol and hemicellulose hydrolysate stimulated lipid accumulation in R. toruloides and R. glutinis, resulting in a shorter fermentation time to reach maximum lipid concentration.
BIOTECHNOLOGY FOR BIOFUELS
(2021)
Article
Green & Sustainable Science & Technology
Man Zhao, Yanan Wang, Wenting Zhou, Wei Zhou, Zhiwei Gong
Summary: Microbial lipid production from low-cost substrates by oleaginous yeasts, such as crude glycerol, shows great potential for commercial success in biodiesel production. The supplementation of additional nutrients and the co-valorization of crude glycerol and other low-cost substrates have been emphasized as strategies to achieve high lipid production. Furthermore, the co-fermentation of glycerol and lignocellulosic sugars has shown synergistic effects for lipogenesis. Integration of crude glycerol-based pretreatment and glycerol/lignocellulosic hydrolysates co-utilization can lead to the development of a novel biodiesel industry. The produced lipids exhibit similar profiles to vegetable oils and possess high-quality biodiesel properties, offering both economic and environmental benefits.
RENEWABLE & SUSTAINABLE ENERGY REVIEWS
(2023)
Article
Thermodynamics
Jiaxin Chen, Xiaolei Zhang, Rajeshwar Dayal Tyagi
Summary: The study demonstrates that cultivating oleaginous yeast in wastewater sludge and crude glycerol medium with the addition of NH4Cl can increase biomass and lipid production, reduce biodiesel production cost, and provide a competitive alternative to commercial biodiesel production from sustainable bioenergy production system.
Article
Green & Sustainable Science & Technology
Raul Robles-Iglesias, Cecilia Naveira-Pazos, Carla Fernandez-Blanco, Maria C. Veiga, Christian Kennes
Summary: The recent increase in energy demand has sparked interest in microbial lipids production as an alternative to traditional fuels due to their high energy density and environmentally-friendly characteristics. Oleaginous yeasts have shown promise in accumulating lipids for biofuel and bioproduct manufacturing. This review provides an overview of common oleaginous species and their ability to accumulate lipids, as well as the effects of different feedstocks on lipid content. Factors such as C/N ratio, pH, and temperature are also analyzed for their impact on lipid production, and genetic modifications are found to significantly increase lipid yield. Cost analysis for scaling-up is included, with feedstock costs being the highest expense.
RENEWABLE & SUSTAINABLE ENERGY REVIEWS
(2023)
Review
Energy & Fuels
Taha Attarbachi, Martin D. Kingsley, Vincenzo Spallina
Summary: The global community's efforts to reduce dependence on fossil fuels have led to an oversupply of crude glycerol due to the increased production of biodiesel. This review paper provides an overview of the global glycerol market, discusses advanced crude glycerol purification technologies, and highlights the challenges and potential solutions for industrial implementation. The results suggest that membrane-based separation technologies driven by pressure, heat, and electrochemistry can effectively purify glycerol, but hybrid solutions may be necessary to address impurities from waste-based feedstocks in biodiesel production.
Article
Environmental Sciences
Derya Berikten, Emir Zafer Hosgun, Ayse Gokdal Otuzbiroglu, Berrin Bozan, Merih Kivanc
Summary: The study found that certain yeast strains can produce lipids from crude glycerol, with Pichia cactophila showing the highest lipid content. Essential fatty acids for biodiesel production were detected in the lipids of these yeast strains.
WASTE AND BIOMASS VALORIZATION
(2021)
Article
Energy & Fuels
Zhiwei Gong, Man Zhao, Qiaoning He, Wei Zhou, Mou Tang, Wenting Zhou
Summary: This study investigated the lipid production of Cutaneotrichosporon oleaginosum from glucose, glycerol, and their mixture. The co-existence of glucose and glycerol improved lipid productivity, possibly due to the enhanced substrate transportation efficiency. Additionally, co-utilization of waste paper enzymatic hydrolysates and crude glycerol resulted in high lipid content and yield.
BIOMASS CONVERSION AND BIOREFINERY
(2022)
Article
Energy & Fuels
Dominika Gyuranova, Vladimir Krasnan, Ivan Spanik, Martin Rebros
Summary: This study successfully expressed recombinant enzymes to degrade biodiesel contaminants using high cell-density fermentation with crude glycerol. The optimized fermentation conditions achieved a biomass concentration of 30 g/l with comparable specific activity to pure glycerol production. The expression of inclusion bodies was eliminated by increasing the induction temperature. The produced enzymes efficiently hydrolyzed biodiesel contaminants and achieved 100% conversion. This approach of utilizing waste carbon recycling for beneficial enzyme production aligns with the concept of sustainable circular bioeconomy.
Article
Engineering, Environmental
Km Sartaj, Shweta Tripathi, Alok Patel, Krishna Mohan Poluri, Ramasare Prasad
Summary: In this study, the halotolerance behavior and carbon flux channelization of a marine red yeast Rhodotorula glutinis ISO A1 cultivated under combinations of artificial seawater and sewage wastewater were investigated to enhance lipid synthesis. The cells grown in 25% artificial seawater showed 1.4-fold higher lipid yield than glucose synthetic medium and exhibited metabolic rewiring to produce neutral lipids. The study revealed the interplay of various metabolites and the active yeast defence network in response to saline stress.
CHEMICAL ENGINEERING JOURNAL
(2023)
Article
Agricultural Engineering
Xiaolei Zhang, Lu Liu, Juan Peng, Fang Yuan, Ji Li, Jiawen Wang, Jiaxin Chen, Hongjie Wang, R. D. Tyagi
Summary: The study found that Cd2+ had a significant impact on lipid accumulation in Lipomyces starkeyi, while other metals had minimal effects. Removing metals from municipal wastewater sludge reduced lipid accumulation, possibly because some metals can enhance lipid accumulation.
BIORESOURCE TECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Haishu Sun, Min Yang, Zhen Gao, Xiaona Wang, Chuanfu Wu, Qunhui Wang, Ming Gao
Summary: In this study, crude glycerol, a byproduct of biodiesel production, was used as a carbon source for microbial lipids production by Rhodotorula toruloides. By optimizing fermentation conditions, the maximum lipid production and lipid content reached 10.56 g/L and 49.52%, respectively. The obtained biodiesel met the standards of China, the United States, and the European Union. The economic value of biodiesel produced from crude glycerol increased by 48% compared with the sale of crude glycerol. In addition, biodiesel production from crude glycerol could reduce 11,928 tons of carbon dioxide emissions and 55 tons of sulfur dioxide emissions.
JOURNAL OF BIOTECHNOLOGY
(2023)
Article
Multidisciplinary Sciences
Yoshiaki Maeda, Kahori Watanabe, Marshila Kaha, Yusuke Yabu, Tomoko Yoshino, Mitsufumi Matsumoto, Tsuyoshi Tanaka
Summary: Microalgae, especially marine oleaginous diatom Fistulifera solaris, are promising producers of biofuel due to their high lipid accumulation. In this study, knocking down Tgl1 gene in F. solaris showed positive impacts on cell growth and lipid accumulation, but the effects were not significant in a two-stage growth system. Changes in fatty acid composition were observed, but comprehensive understanding of lipid catabolism is crucial for further improving lipid production.
SCIENTIFIC REPORTS
(2021)
Article
Energy & Fuels
Mohamed Guerfali, Ines Ayadi, Wajdi Ayadi, Slim Smaoui, Khaoula Elhadef, Hatem Zaghden, Lobna Jlaiel, Emna Sahli, Hafedh Belghith, Ali Gargouri
Summary: Yeasts, especially red yeasts, are attracting significant attention as major sources of value-added metabolites, such as microbial lipids and carotenoids, which have various biological activities and can be used as biofuels, oleo-chemicals, and food additives. This study focuses on using biodiesel-derived crude glycerol as a feedstock for producing valuable metabolites by the oleaginous yeast Rhodotorula babjevae Y-SL7. The study successfully established the optimal conditions for maximum production of lipids and carotenoids. The extracted carotenoids were found to have antimicrobial and antioxidant activities, and the secreted polyol esters of fatty acids (PEFAs) showed cytotoxic effects against cancer cells, with a synergistic action observed when combined with a commercial drug. Additionally, culture on crude glycerol was shown to enhance metabolite recovery through increased cells membrane permeability.
BIOMASS CONVERSION AND BIOREFINERY
(2022)
Article
Biotechnology & Applied Microbiology
Nikolaus I. Stellner, Zora S. Rerop, Norbert Mehlmer, Mahmoud Masri, Marion Ringel, Thomas B. Brueck
Summary: The study analyzed the structure of putative endogenous promoter sequences of Cutaneotrichosporon oleaginosus based on highly expressed genes and identified motifs and translational initiation sites. Four newly described promoters along with previously established ones successfully initiated gene expression, with PPIp chosen for further marker development. Two dominant resistance markers, geneticin G418 resistance and nourseothricin resistance gene N-acetyl transferase, showed high transformation efficiency and compatibility for combined use in genetic engineering and strain development.
Article
Biotechnology & Applied Microbiology
Sara Moreno-Paz, Joep Schmitz, Vitor A. P. Martins dos Santos, Maria Suarez-Diez
Summary: This study assessed the accuracy and predictive power of genome-scale, constraint-based models in predicting metabolic changes in response to operational conditions in a bioreactor and intracellular, active reactions. The findings showed that the enzyme-constrained version of the model outperformed the original model in all simulations. The combination of this model with dynamic FBA allowed for the prediction of yields and productivities of different strains and production processes. Additionally, constraining protein availability improved the accuracy of the metabolic state description under dynamic conditions. These findings have important implications for the design of industrially relevant cell-based processes.
MICROBIAL BIOTECHNOLOGY
(2022)
Article
Agriculture, Multidisciplinary
Lenny Ferrer, Melanie Mindt, Maria Suarez-Diez, Tatjana Jilg, Maja Zagorscak, Jin-Ho Lee, Kristina Gruden, Volker F. Wendisch, Katarina Cankar
Summary: Indole, a compound with a characteristic odor, is produced by various organisms in nature and plays a role in food flavor and fragrance industry. Researchers discovered a bacterial enzyme that can synthesize indole, leading to an increase in indole production.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Biotechnology & Applied Microbiology
Melanie Mindt, Arman Beyraghdar Kashkooli, Maria Suarez-Diez, Lenny Ferrer, Tatjana Jilg, Dirk Bosch, Vitor Martins dos Santos, Volker F. Wendisch, Katarina Cankar
Summary: The biotechnological production process established in this study provides an attractive route for sustainable indole production from tryptophan in C. glutamicum. Industrially relevant indole titers were achieved within 24 hours and indole was concentrated in the organic layer as a pure product after fermentation.
MICROBIAL CELL FACTORIES
(2022)
Article
Multidisciplinary Sciences
Wasin Poncheewin, Anne D. van Diepeningen, Theo A. J. van der Lee, Maria Suarez-Diez, Peter J. Schaap
Summary: This study used genome properties and machine learning to establish the relationship between Pseudomonas strains isolated from the rhizosphere and phyllosphere and their plant-associated lifestyle. It identified 28 discriminating features and demonstrated the potential of genome properties annotation as a computational tool for classifying plant-associated lifestyles.
SCIENTIFIC REPORTS
(2022)
Article
Biochemical Research Methods
Maksim Zakhartsev, Filip Rotnes, Marie Gulla, Ove Oyas, Jesse C. J. van Dam, Maria Suarez-Diez, Fabian Grammes, Robert Anton Hafthorsson, Wout van Helvoirt, Jasper J. Koehorst, Peter J. Schaap, Yang Jin, Liv Torunn Mydland, Arne B. Gjuvsland, Simen R. Sandve, Vitor A. P. Martins dos Santos, Jon Olav Vik
Summary: SALARECON is a model that links the genome of Atlantic salmon to metabolic fluxes and growth, allowing for the simulation of metabolism and growth. It can be used to explain Atlantic salmon physiology and address key challenges in aquaculture.
PLOS COMPUTATIONAL BIOLOGY
(2022)
Article
Microbiology
Warasirin Sornlek, Kittapong Sae-Tang, Akaraphol Watcharawipas, Sriwan Wongwisansri, Sutipa Tanapongpipat, Lily Eurwilaichtr, Verawat Champreda, Weerawat Runguphan, Peter J. Schaap, Vitor A. P. Martins Dos Santos
Summary: In this study, the production of D-lactic acid (D-LA) was improved in Saccharomyces cerevisiae by gene editing and conventional breeding. By deleting specific genes and crossbreeding with acid-tolerant yeast strains, the production and yield of D-LA were significantly increased, while the production of glycerol and ethanol were minimized.
Article
Microbiology
Sara Benito-Vaquerizo, Niels Nouse, Peter J. Schaap, Jeroen Hugenholtz, Stanley Brul, Ana M. Lopez-Contreras, Vitor A. P. Martins dos Santos, Maria Suarez-Diez
Summary: This study designed a new co-culture of an acetogen and a solventogen using metabolic modeling to produce butyrate from CO2. The experimental results confirmed the feasibility and stability of the predicted microbial consortium and showed the value of community modeling in guiding the design of microbial consortia for chemical production from renewable resources.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Fisheries
Pacharaporn Angthong, Tanaporn Uengwetwanit, Umaporn Uawisetwathana, Jasper J. Koehorst, Sopacha Arayamethakorn, Peter J. Schaap, Vitor Martins Dos Santos, Metavee Phromson, Nitsara Karoonuthaisiri, Sage Chaiyapechara, Wanilada Rungrassamee
Summary: To understand the interaction between shrimp and intestinal microbiota, multidisciplinary platforms were used to analyze the metagenome, transcriptome, and metabolome. The study found that exposure to Vibrio harveyi disrupted the bacterial communities in the shrimp intestine. Gene expression analysis revealed upregulation of immune-related pathways, while metabolomics analysis showed changes in lipid metabolism. The findings suggest the importance of lipid metabolism and the impact of pathogenic Vibrio on shrimp immunity. This study provides valuable insights into the host-gut microbial relationship and its influence on shrimp innate immunity.
Article
Biochemistry & Molecular Biology
Toshihiko Katoh, Chihaya Yamada, Michael D. Wallace, Ayako Yoshida, Aina Gotoh, Moe Arai, Takako Maeshibu, Toma Kashima, Arno Hagenbeek, Miriam N. Ojima, Hiromi Takada, Mikiyasu Sakanaka, Hidenori Shimizu, Keita Nishiyama, Hisashi Ashida, Junko Hirose, Maria Suarez-Diez, Makoto Nishiyama, Ikuo Kimura, Keith A. Stubbs, Shinya Fushinobu, Takane Katayama
Summary: The BbhII enzyme from Bifidobacterium bifidum is involved in the breakdown of mucin O-glycans, modulating host-microbiota symbiosis and dysbiosis.
NATURE CHEMICAL BIOLOGY
(2023)
Article
Microbiology
Thanaporn Laothanachareon, Enrique Asin-Garcia, Rita J. M. Volkers, Juan Antonio Tamayo-Ramos, Vitor A. P. Martins dos Santos, Peter J. Schaap
Summary: Aspergillus niger is a powerful microbial cell factory for producing organic acids. The regulation of the glucose oxidase (Gox) expression system, which is involved in gluconic acid biosynthesis, has been recently discovered. The study found that hydrogen peroxide plays a crucial role as a signaling molecule in inducing this system. The facilitated diffusion of hydrogen peroxide via aquaporin water channels (AQPs) was studied in this research.
Article
Biochemical Research Methods
William T. R. Scott Jr, Sara Benito-Vaquerizo, Johannes R. Zimmermann, Djordje Bajic, Almut R. Heinken, Maria Suarez-Diez, Peter R. Schaap
Summary: This study examined and evaluated 24 constraint-based modeling tools for genome-scale metabolic modeling of microbial consortia. It found that newer, more accessible, and well-documented tools generally outperformed older tools, but some older tools had tradeoffs in terms of accuracy or flexibility. The study provides recommendations for researchers in choosing the most suitable tools and opportunities for improvement in metabolic modeling of multi-species microbial consortia.
PLOS COMPUTATIONAL BIOLOGY
(2023)
Article
Biology
Bart Nijsse, Peter J. Schaap, Jasper J. Koehorst
Summary: The life sciences are a major source of scientific data. Reusing and connecting these data can lead to new concepts and insights. However, the availability of easy-to-adopt implementations that fulfill the needs of data producers is limited. In response, the FAIR Data Station, a lightweight application written in Java, has been developed to support researchers in managing research metadata according to the FAIR principles. It includes modules for generating metadata templates, validating recorded values, and converting metadata into RDF format.
Correction
Biology
Andra Waagmeester, Egon L. Willighagen, Andrew I. Su, Martina Kutmon, Jose Emilio Labra Gayo, Daniel Fernandez-Alvarez, Quentin Groom, Peter J. Schaap, Lisa M. Verhagen, Jasper J. Koehorst
Article
Immunology
Nhung Pham, Finterly Hu, Chris T. Evelo, Martina Kutmon
Summary: This study mapped proteomics data to pathway models and revealed the impact of COVID-19 on pathway activities in different tissues. The results indicate that COVID-19 affects not only the lung but also other tissues systemically.
FRONTIERS IN IMMUNOLOGY
(2022)