Article
Pharmacology & Pharmacy
Cong Bang Truong, Spencer H. Durham, Jingjing Qian
Summary: The study revealed higher reporting rates of serious adverse events and acute kidney diseases for colistin compared to polymyxin B, but no difference in death rates. These findings support the need for future research with stronger study designs and larger sample sizes to compare the safety profiles of colistin and polymyxin B.
EXPERT OPINION ON DRUG SAFETY
(2021)
Article
Environmental Sciences
Sirinthrar Wandee, Rathborey Chan, Wilai Chiemchaisri, Chart Chiemchaisri
Summary: The study found that in open pig farming systems, there is a high level of E. coli populations, antibiotic contamination in supplied water, and additional antibiotic usage, such as neomycin or colistin. Additionally, an increased occurrence of ARE was found in anaerobic digestion and waste stabilisation ponds, but they were not well correlated with their residual antibiotic concentrations.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Article
Biochemical Research Methods
Corrie R. Belanger, Robert E. W. Hancock
Summary: This study presents a protocol for testing physiologically relevant infection conditions in minimal inhibitory concentration (MIC) assays, which can be used to assess the susceptibility of bacterial isolates and novel antimicrobial drugs under conditions more similar to clinical infections. The protocol can be applied to most physiologically relevant liquid medium and aerobic pathogens, taking 3-4 days to complete, and may provide a more accurate assessment of the bactericidal effects of new drugs.
Article
Engineering, Biomedical
Arne Matteo Joergensen, Christian Steinbring, Daniel Stengel, Dennis To, Pascal Schmid, Andreas Bernkop-Schnuerch
Summary: This study developed self-emulsifying drug delivery systems containing dry reverse micelles (SEDDS-dRMsPMB) loaded with the model peptide drug polymyxin B (PMB) using anionic (AOT), cationic (DODAB), amphoteric (SL), or non-ionic (P85) surfactants. These systems showed high payload, efficient cellular uptake, and maintained antimicrobial activity, suggesting a potential game-changing strategy for oral peptide drug delivery.
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Microbiology
Amanda Holstad Singleton, Olaug Elisabeth Torheim Bergum, Caroline Krogh Sogaard, Lisa Marie Rost, Cecilie Elisabeth Olsen, Fredrik Heen Blindheim, Synnove Brandt Raeder, Frithjof A. Bjornstad, Eirik Sundby, Bard Helge Hoff, Per Bruheim, Marit Otterlei
Summary: The increasing number of infections caused by antibiotic resistant bacteria in recent decades has led to a demand for new antibiotics and drug combinations to reduce resistance development. The combination of the antimicrobial peptide BTP-001 and the pyrrolopyrimidine JK-274 has shown a strong additive effect, likely due to the activation of multiple stress responses. These findings demonstrate the potential of JK-274 and BTP-001 as antibiotic candidates and call for further studies.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Materials Science, Multidisciplinary
Yanan Xu, Zhenhua Li, Dan Xiu, Guotao Sun, Christopher D. Snow, Yao Wang, Yanxin Wang, Laurence A. Belfiore, Jianguo Tang
Summary: The histidine polypeptide-hybridized nanoscale metal-organic framework can be effectively produced in water at room temperature without additives, and it exhibits useful properties of dissolution and controlled release in low pH conditions. Furthermore, it increases uptake by cancer cells, opening a new route for advanced drug delivery systems.
MATERIALS & DESIGN
(2021)
Article
Microbiology
Rundong Tan, Anqi Yu, Ziming Liu, Ziqi Liu, Rongfeng Jiang, Xiaoli Wang, Jialin Liu, Junhui Gao, Xinjun Wang
Summary: MIC is crucial for bacterial detection in infections, and predicting MIC accurately using genome sequencing and machine learning algorithms can improve clinical efficacy and antibiotic use.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Microbiology
Andras Kallai, Marta Kelemen, Noemi Molnar, Adrienn Tropotei, Balazs Hauser, Zsolt Ivanyi, Janos Gal, Erzsebet Ligeti, Katalin Kristof, Akos M. Lorincz
Summary: The study developed a flow cytometry-based antibiotic susceptibility testing (AST) method, which can produce reliable results a workday earlier than the traditional microdilution method. Results showed that the method is able to detect bacterial growth accurately after 4 hours of incubation, with high consistency compared to the microdilution method.
MICROBIOLOGY SPECTRUM
(2021)
Article
Multidisciplinary Sciences
Christin Pohl, Gregory Effantin, Eaazhisai Kandiah, Sebastian Meier, Guanghong Zeng, Werner Streicher, Dorotea Raventos Segura, Per H. Mygind, Dorthe Sandvang, Line Anker Nielsen, Gunther H. J. Peters, Guy Schoehn, Christoph Mueller-Dieckmann, Allan Noergaard, Pernille Harris
Summary: This study reports the cryo-EM structure of a triple-mutant of the antimicrobial peptide plectasin, which assembles into helical non-amyloid fibrils. The fibril formation is reversible and pH- and concentration-dependent. This finding reveals that alpha/beta proteins can naturally assemble into fibrils.
NATURE COMMUNICATIONS
(2022)
Review
Microbiology
Beata Kowalska-Krochmal, Ruth Dudek-Wicher
Summary: The inefficiency of medical therapies for curing patients with bacterial infections calls for active search for new therapeutic strategies and careful selection of antibiotics based on various parameters including microbiological considerations. Proper assessment of Minimal Inhibitory Concentration (MIC) is crucial for optimizing treatment strategies and ensuring the effectiveness of infection therapy. This paper discusses methods for MIC estimation, factors influencing MIC results, and possibilities of utilizing MIC in clinical practice with consideration of pharmacokinetic/pharmacodynamic parameters.
Article
Immunology
Wei Feng, Lei Zhang, Qian Yuan, Yu Wang, Pu Yao, Peiyuan Xia, Fengjun Sun
Summary: The study demonstrated that sub-minimal inhibitory concentration of ceftazidime can reduce the pathogenicity of UPEC by inhibiting adhesion ability, biofilm formation, swimming and swarming motilities, and decreasing hemolytic activity and cytotoxicity. CAZ also affects UPEC virulence and biofilm formation by downregulating important genes, leading to reduced adhesion to human bladder epithelial cells and less severe UTI symptoms.
MICROBIAL PATHOGENESIS
(2021)
Article
Biology
Cristina Rodriguez-Melcon, Carlos Alonso-Calleja, Camino Garcia-Fernandez, Javier Carballo, Rosa Capita
Summary: This study determined the lethal concentrations of disinfectants and antibiotics for Listeria monocytogenes and found that the surface characteristics of bacterial cells influence resistance to these compounds. Most strains showed resistance to antibiotics, highlighting the concern of antibiotic resistance.
Article
Critical Care Medicine
Nakwon Kwak, Jake Whang, Jeong Seong Yang, Taek Soo Kim, Sung A. Kim, Jae-Joon Yim
Summary: Clofazimine MIC values varied widely in clinical isolates from NTM-PD patients, and negative conversion of sputum culture with clofazimine use was associated with lower MIC values. Clofazimine use could be considered in patients with NTM-PD when the MIC value is <= 0.25 mg/L.
Article
Health Care Sciences & Services
Clara Grazian
Summary: Antimicrobial resistance poses a significant threat to global public health. Researchers are using whole-genome sequencing to develop personalized treatments and improve prediction of resistance and treatment recommendations. Robust methods are needed to identify resistance-associated mutations.
STATISTICAL METHODS IN MEDICAL RESEARCH
(2023)
Article
Chemistry, Multidisciplinary
Shuying Wang, Griffin Downing, Karl F. Olsen, Tomi K. Sawyer, Roger D. Cone, Steven P. Schwendeman
Summary: This study developed a long-acting release formulation for setmelanotide using remote loading into PLGA microspheres. The loaded microspheres demonstrated slow and continuous peptide release, leading to long-term inhibition of food intake and body weight control in a diet induced obese murine model. This provides a promising treatment option for obesity.
JOURNAL OF CONTROLLED RELEASE
(2023)
Article
Biochemistry & Molecular Biology
Valentina A. Petrova, Natallia V. Dubashynskaya, Iosif V. Gofman, Alexey S. Golovkin, Alexander I. Mishanin, Arthur D. Aquino, Daria V. Mukhametdinova, Alexandra L. Nikolaeva, Elena M. Ivan'kova, Alexander E. Baranchikov, Alexander V. Yakimansky, Vladimir K. Ivanov, Yury A. Skorik
Summary: Polymeric nanocomposite materials have the potential to enhance tissue-engineered scaffolds by influencing the structure and properties of polymeric materials and regulating cell behavior. In this study, cerium oxide nanoparticles were incorporated into a chitosan film to promote the proliferation of mesenchymal stem cells. The resulting CS-CeONP films exhibited good biocompatibility and regenerative potential in both in vitro and in vivo experiments.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Biochemistry & Molecular Biology
Natallia V. V. Dubashynskaya, Anton N. N. Bokatyi, Anatoliy V. V. Dobrodumov, Igor V. V. Kudryavtsev, Andrey S. S. Trulioff, Artem A. A. Rubinstein, Arthur D. D. Aquino, Yaroslav A. A. Dubrovskii, Elena S. S. Knyazeva, Elena V. V. Demyanova, Yuliya A. A. Nashchekina, Yury A. A. Skorik
Summary: The growth of microbial multidrug resistance is a problem in modern clinical medicine. Chemical modification of active pharmaceutical ingredients is an attractive strategy to improve their biopharmaceutical properties. Conjugation of antimicrobial drugs with natural polysaccharides provides targeted delivery, controlled drug release, and reduced toxicity. The study synthesized colistin conjugates with succinyl chitosan and found promising results in terms of drug release, antimicrobial activity, and reduction of drug nephrotoxicity and inflammation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Biochemistry & Molecular Biology
Natallia V. V. Dubashynskaya, Ekaterina R. R. Gasilova, Yury A. A. Skorik
Summary: The marine polysaccharide fucoidan (FUC) shows great potential in pharmaceutical research for developing drug delivery systems with modified release and targeted delivery. The presence of a sulfate group in FUC makes it suitable for forming interpolyelectrolyte complexes (PECs) with various polycations. However, the structural diversity of FUC poses challenges in designing FUC-based nanoformulations.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Apollinariia Yu. Dzhuzha, Irina I. I. Tarasenko, Leonard Ionut Atanase, Antonina Lavrentieva, Evgenia G. G. Korzhikova-Vlakh
Summary: Synthetic poly(amino acids) are macromolecules that mimic natural polypeptides and are commonly used as carriers for drug and gene delivery. In this study, we synthesized and characterized amphiphilic copolymers by modifying poly(alpha,L-glutamic acid) with hydrophobic and basic L-amino acids and D-glucosamine. The resulting glycopolypeptides formed nanoparticles that showed reduced macrophage uptake and were non-toxic to human lung epithelial cells. These nanoparticles were also capable of loading hydrophobic drugs, such as paclitaxel, and demonstrated high cytostatic efficacy against human lung adenocarcinoma.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Valentina A. A. Petrova, Iosif V. V. Gofman, Natallia V. V. Dubashynskaya, Alexey S. S. Golovkin, Alexander I. I. Mishanin, Elena M. M. Ivan'kova, Dmitry P. P. Romanov, Albert K. K. Khripunov, Elena N. N. Vlasova, Alexandra V. V. Migunova, Alexander E. E. Baranchikov, Vladimir K. K. Ivanov, Alexander V. V. Yakimansky, Yury A. A. Skorik
Summary: New composite films were prepared by incorporating bacterial cellulose nanofibers and cerium oxide nanoparticles into chitosan matrices. The amount of nanofillers affected the structure and properties of the composites, including film stiffness and strength. Increasing the concentration of nanoceria improved the biocompatibility and adhesion of the films to mesenchymal stem cells. These nanocomposite films exhibit favorable properties for use as matrix materials in cell culture and wound dressings.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Natalia Zashikhina, Sergei Gladnev, Vladimir Sharoyko, Viktor Korzhikov-Vlakh, Evgenia Korzhikova-Vlakh, Tatiana Tennikova
Summary: The covalent conjugation of dexamethasone with polymeric carriers is a promising strategy to overcome its limitations. In this study, self-assembling amphiphilic polypeptides were proposed as potential delivery systems for intravitreal delivery. Nanoparticles were prepared using different polypeptides and characterized in terms of size, polydispersity, and zeta potential.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell Biology
Sandra Dienemann, Vanessa Schmidt, Tabea Fleischhammer, Julia H. H. Mueller, Antonina Lavrentieva
Summary: In vitro cultivation conditions are important for understanding cell physiology and responses to external stimuli. Oxygen concentrations are crucial in influencing cell behaviors in both 2D and 3D cultures. This study genetically modified two types of human endothelial cells and monitored their responses to different oxygen concentrations. The findings demonstrated different hypoxic signatures for these cells in both 2D and 3D culture systems.
JOURNAL OF CELLULAR PHYSIOLOGY
(2023)
Article
Chemistry, Physical
Anastasia A. Belyaeva, Ilya V. V. Tretyakov, Alexey V. Kireynov, Yuliya A. Nashchekina, Vitaliy I. Solodilov, Evgenia G. Korzhikova-Vlakh, Sofia M. Morozova
Summary: The colloidal system based on a mixture of PNIPAM and CNC was studied, showing different states and properties at different temperatures. By controlling the ratio between PNIPAM and CNC, the system's state and properties can be adjusted, making these hydrogels promising materials for bioapplications such as extrusion printing.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
R. G. Sakhabeev, D. S. Polyakov, E. S. Sinitsyna, E. G. Korzhikova-Vlakh, V. A. Korzhikov-Vlakh, M. M. Shavlovsky
Summary: The effect of the composition and size of polymeric particles on the immunogenicity of beta 2-microglobulin protein was studied. Nanoparticles and microparticles showed different impacts on immune response.
JOURNAL OF EVOLUTIONARY BIOCHEMISTRY AND PHYSIOLOGY
(2023)
Article
Polymer Science
Natallia V. V. Dubashynskaya, Valentina A. A. Petrova, Andrey V. V. Sgibnev, Vladimir Y. Y. Elokhovskiy, Yuliya I. I. Cherkasova, Yury A. A. Skorik
Summary: The development of cryogels containing metronidazole (MET) based on carrageenan (CRG) and partially deacetylated chitin nanowhiskers (CNWs) has shown promise for improved vaginal delivery. The incorporation of CNWs significantly improved the strength and homogeneity of the cryogels, resulting in sustained drug release within 24 hours. The mechanism of prolonged release was related to polymer swelling and chain relaxation in the matrix, as demonstrated by the Korsmeyer-Peppas and Peppas-Sahlin models. These cryogels exhibited a prolonged antiprotozoal effect, making them potential dosage forms for the treatment of vaginal infections.
Article
Biochemistry & Molecular Biology
Natallia V. Dubashynskaya, Anton N. Bokatyi, Tatiana S. Sall, Tatiana S. Egorova, Yuliya A. Nashchekina, Yaroslav A. Dubrovskii, Ekaterina A. Murashko, Elena N. Vlasova, Elena V. Demyanova, Yury A. Skorik
Summary: Polymeric drug delivery systems enhance the properties of antibiotics by increasing bioavailability, providing controlled-release, and reducing toxicity. This study describes the modification of conjugates based on colistin and hyaluronic acid with vitamin B12, which improved intestinal permeability, achieved slow release, and retained antimicrobial activity. These vitamin B12-modified conjugates may be promising oral delivery systems with improved biopharmaceutical properties.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Pharmacology & Pharmacy
Natallia V. Dubashynskaya, Anton N. Bokatyi, Andrey S. Trulioff, Artem A. Rubinstein, Igor V. Kudryavtsev, Yury A. Skorik
Summary: This study aimed to develop a dexamethasone phosphate delivery system based on hyaluronic acid and a cationic chitosan derivative, which showed both excellent mucoadhesion and prolonged drug release.
Article
Chemistry, Physical
Gulia Bikbaeva, Anastasia Egorova, Nikolai Sonin, Anna Pilip, Ilya Kolesnikov, Dmitrii Pankin, Roman Boroznjak, Alina Manshina
Summary: Photoswitchable molecules with bioactive properties are highly sought after in various fields, especially in biomedicine. This study presents vinyl phosphonates that can undergo laser-induced cis-trans isomerization via a C=C bond. The unique advantage of these vinyl phosphonates is their bioactivity, unlike other photoswitchable molecules. The butyrylcholinesterase inhibition by these compounds was found to significantly increase after laser irradiation. This opens up promising applications for vinyl phosphonates as photopharmacological agents.
Article
Chemistry, Analytical
Akinrinade George Ayankojo, Roman Boroznjak, Jekaterina Reut, Jurgen Tuvikene, Tonis Timmusk, Vitali Syritski
Summary: This study presents an electrochemical sensor based on a molecularly imprinted polymer for rapid detection of the neurodegenerative disorder biomarker BDNF. The sensor demonstrates high selectivity and sensitivity, and shows potential for real sample analysis.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
