CRISPR-Cas12a (Cpf1): A Versatile Tool in the Plant Genome Editing Tool Box for Agricultural Advancement

The rapidly advancing Class 2 CRISPR‐Cas technologies: A customizable toolbox for molecular manipulations

(2020) Jingyi Wang et al.   JOURNAL OF CELLULAR AND MOLECULAR MEDICINE

Improved LbCas12a variants with altered PAM specificities further broaden the genome targeting range of Cas12a nucleases

(2020) Eszter Tóth et al.   NUCLEIC ACIDS RESEARCH

Comparison of CRISPR-Cas9/Cas12a Ribonucleoprotein Complexes for Genome Editing Efficiency in the Rice Phytoene Desaturase (OsPDS) Gene

(2020) Raviraj Banakar et al.   Rice

The evolving landscape around genome editing in agriculture

(2020) Sarah M Schmidt et al.   EMBO REPORTS

Conventional and Molecular Techniques from Simple Breeding to Speed Breeding in Crop Plants: Recent Advances and Future Outlook

(2020) Sunny Ahmar et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

CRISPR-Cas12a has widespread off-target and dsDNA-nicking effects

(2020) Karthik Murugan et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Prime genome editing in rice and wheat

(2020) Qiupeng Lin et al.   NATURE BIOTECHNOLOGY

Highly efficient homology‐directed repair using CRISPR/Cpf1‐geminiviral replicon in tomato

(2020) Tien Van Vu et al.   PLANT BIOTECHNOLOGY JOURNAL

Plant genome editing and the relevance of off-target changes

(2020) Nathaniel Graham et al.   PLANT PHYSIOLOGY

Unconstrained genome targeting with near-PAMless engineered CRISPR-Cas9 variants

(2020) Russell T. Walton et al.   SCIENCE

Complex Trait Loci in Maize Enabled by CRISPR-Cas9 Mediated Gene Insertion

(2020) Huirong Gao et al.   Frontiers in Plant Science

Prime Editing: Game Changer for Modifying Plant Genomes

(2020) Marek Marzec et al.   TRENDS IN PLANT SCIENCE

Cas12a Base Editors Induce Efficient and Specific Editing with Low DNA Damage Response

(2020) Xiao Wang et al.   Cell Reports

New Food Crop Domestication in the Age of Gene Editing: Genetic, Agronomic and Cultural Change Remain Co-evolutionarily Entangled

(2020) David L. Van Tassel et al.   Frontiers in Plant Science

CRISPR ‐LbCas12a‐mediated modification of citrus

(2019) Hongge Jia et al.   PLANT BIOTECHNOLOGY JOURNAL

Precise gene replacement in rice by RNA transcript-templated homologous recombination

(2019) Shaoya Li et al.   NATURE BIOTECHNOLOGY

Engineered CRISPR–Cas12a variants with increased activities and improved targeting ranges for gene, epigenetic and base editing

(2019) Benjamin P. Kleinstiver et al.   NATURE BIOTECHNOLOGY

One-step genome editing of elite crop germplasm during haploid induction

(2019) Timothy Kelliher et al.   NATURE BIOTECHNOLOGY

Improving plant genome editing with high-fidelity xCas9 and non-canonical PAM-targeting Cas9-NG

(2019) Zhaohui Zhong et al.   Molecular Plant

Development of a Haploid-Inducer Mediated Genome Editing System for Accelerating Maize Breeding

(2019) Baobao Wang et al.   Molecular Plant

Application of CRISPR-Cas12a temperature sensitivity for improved genome editing in rice, maize, and Arabidopsis

(2019) Aimee A. Malzahn et al.   BMC BIOLOGY

CRISPR/Cas Genome Editing and Precision Plant Breeding in Agriculture

(2019) Kunling Chen et al.   Annual Review of Plant Biology

Development and Application of CRISPR/Cas System in Rice

(2019) Ren Jun et al.   Rice Science

Assessment of Cas12a‐mediated gene editing efficiency in plants

(2019) Joan Miquel Bernabé‐Orts et al.   PLANT BIOTECHNOLOGY JOURNAL

Robust CRISPR /Cpf1(Cas12a) mediated genome editing in allotetraploid cotton ( G. hirsutum )

(2019) Bo Li et al.   PLANT BIOTECHNOLOGY JOURNAL

CRISPR Cpf1 proteins: structure, function and implications for genome editing

(2019) Fatemeh Safari et al.   Cell and Bioscience

The Problem of the Low Rates of CRISPR/Cas9-Mediated Knock-ins in Plants: Approaches and Solutions

(2019) Serge M. Rozov et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Modern Trends in Plant Genome Editing: An Inclusive Review of the CRISPR/Cas9 Toolbox

(2019) Ali Razzaq et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

In planta gene targeting can be enhanced by the use of CRISPR/Cas12a

(2019) Felix Wolter et al.   PLANT JOURNAL

CRISPR-based tools for targeted transcriptional and epigenetic regulation in plants

(2019) Joanne E. Lee et al.   PLoS One

Making the cut(s): how Cas12a cleaves target and non-target DNA

(2019) Daan C. Swarts   BIOCHEMICAL SOCIETY TRANSACTIONS

Efficient induction of haploid plants in wheat by editing of TaMTL using an optimized Agrobacterium-mediated CRISPR system

(2019) Huiyun Liu et al.   JOURNAL OF EXPERIMENTAL BOTANY

Search-and-replace genome editing without double-strand breaks or donor DNA

(2019) Andrew V. Anzalone et al.   NATURE

Engineering CRISPR/ Lb Cas12a for highly efficient, temperature‐tolerant plant gene editing

(2019) Patrick Schindele et al.   PLANT BIOTECHNOLOGY JOURNAL

CRISPR‐Cas12a enables efficient biallelic gene targeting in rice

(2019) Shaoya Li et al.   PLANT BIOTECHNOLOGY JOURNAL

CRISPR/Cas brings plant biology and breeding into the fast lane

(2019) Angelina Schindele et al.   CURRENT OPINION IN BIOTECHNOLOGY

The Biology of CRISPR-Cas: Backward and Forward

(2018) Frank Hille et al.   CELL

Synthesis-dependent repair of Cpf1-induced double strand DNA breaks enables targeted gene replacement in rice

(2018) Shaoya Li et al.   JOURNAL OF EXPERIMENTAL BOTANY

Robust Transcriptional Activation in Plants Using Multiplexed CRISPR-Act2.0 and mTALE-Act Systems

(2018) Levi G. Lowder et al.   Molecular Plant

Plant Genome Editing Using FnCpf1 and LbCpf1 Nucleases at Redefined and Altered PAM Sites

(2018) Zhaohui Zhong et al.   Molecular Plant

Expanding the Scope of CRISPR/Cpf1-Mediated Genome Editing in Rice

(2018) Shaoya Li et al.   Molecular Plant

Chemically Modified Cpf1-CRISPR RNAs Mediate Efficient Genome Editing in Mammalian Cells

(2018) Moira A. McMahon et al.   MOLECULAR THERAPY

Activities and specificities of CRISPR-Cas9 and Cas12a nucleases for targeted mutagenesis in maize

(2018) Keunsub Lee et al.   PLANT BIOTECHNOLOGY JOURNAL

Small molecules promote CRISPR-Cpf1-mediated genome editing in human pluripotent stem cells

(2018) Xiaojie Ma et al.   Nature Communications

True gene-targeting events by CRISPR/Cas-induced DSB repair of the PPO locus with an ectopically integrated repair template

(2018) Sylvia de Pater et al.   Scientific Reports

Cas9 versus Cas12a/Cpf1: Structure-function comparisons and implications for genome editing

(2018) Daan C. Swarts et al.   Wiley Interdisciplinary Reviews-RNA

Homology-Directed Repair of a Defective Glabrous Gene in Arabidopsis With Cas9-Based Gene Targeting

(2018) Florian Hahn et al.   Frontiers in Plant Science

Functional Genetic Variants Revealed by Massively Parallel Precise Genome Editing

(2018) Eilon Sharon et al.   CELL

CRISPR plants now subject to tough GM laws in European Union

(2018) Ewen Callaway   NATURE

Mb- and FnCpf1 nucleases are active in mammalian cells: activities and PAM preferences of four wild-type Cpf1 nucleases and of their altered PAM specificity variants

(2018) Eszter Tóth et al.   NUCLEIC ACIDS RESEARCH

Highly efficient genome editing by CRISPR-Cpf1 using CRISPR RNA with a uridinylate-rich 3′-overhang

(2018) Su Bin Moon et al.   Nature Communications

Extension of the crRNA enhances Cpf1 gene editing in vitro and in vivo

(2018) Hyo Min Park et al.   Nature Communications

Genome Editing in Rice: Recent Advances, Challenges, and Future Implications

(2018) Rukmini Mishra et al.   Frontiers in Plant Science

Programmable DNA repair with CRISPRa/i enhanced homology-directed repair efficiency with a single Cas9

(2018) Lupeng Ye et al.   Cell Discovery

Single transcript unit CRISPR 2.0 systems for robust Cas9 and Cas12a mediated plant genome editing

(2018) Xu Tang et al.   PLANT BIOTECHNOLOGY JOURNAL

Enhanced genome editing in rice using single transcript unit CRISPR-Lb Cpf1 systems

(2018) Rongfang Xu et al.   PLANT BIOTECHNOLOGY JOURNAL

Improvement of the CRISPR-Cpf1 system with ribozyme-processed crRNA

(2018) Zongliang Gao et al.   RNA Biology

Targeted mutagenesis in rice using CRISPR-Cpf1 system

(2017) Xixun Hu et al.   Journal of Genetics and Genomics

Gene Targeting by Homology-Directed Repair in Rice Using a Geminivirus-Based CRISPR/Cas9 System

(2017) Mugui Wang et al.   Molecular Plant

Multiplex Gene Editing in Rice Using the CRISPR-Cpf1 System

(2017) Mugui Wang et al.   Molecular Plant

Engineered Cpf1 variants with altered PAM specificities

(2017) Linyi Gao et al.   NATURE BIOTECHNOLOGY

Generation of targeted mutant rice using a CRISPR-Cpf1 system

(2017) Rongfang Xu et al.   PLANT BIOTECHNOLOGY JOURNAL

CRISPR-Cas9 and CRISPR-Cpf1 mediated targeting of a stomatal developmental gene EPFL9 in rice

(2017) Xiaojia Yin et al.   PLANT CELL REPORTS

CRISPR-Cas: Adapting to change

(2017) Simon A. Jackson et al.   SCIENCE

CRISPR-Cpf1 mediates efficient homology-directed repair and temperature-controlled genome editing

(2017) Miguel A. Moreno-Mateos et al.   Nature Communications

CRISPR/Cpf1-mediated DNA-free plant genome editing

(2017) Hyeran Kim et al.   Nature Communications

Multiplex gene regulation by CRISPR-ddCpf1

(2017) Xiaochun Zhang et al.   Cell Discovery

Engineering CRISPR–Cpf1 crRNAs and mRNAs to maximize genome editing efficiency

(2017) Bin Li et al.   Nature Biomedical Engineering

Precise insertion and guided editing of higher plant genomes using Cpf1 CRISPR nucleases

(2017) Matthew B. Begemann et al.   Scientific Reports

Trends in Approval Times for Genetically Engineered Crops in the United States and the European Union

(2016) Richard D. Smart et al.   JOURNAL OF AGRICULTURAL ECONOMICS

Identifying and Visualizing Functional PAM Diversity across CRISPR-Cas Systems

(2016) Ryan T. Leenay et al.   MOLECULAR CELL

Engineering Herbicide-Resistant Rice Plants through CRISPR/Cas9-Mediated Homologous Recombination of Acetolactate Synthase

(2016) Yongwei Sun et al.   Molecular Plant

The crystal structure of Cpf1 in complex with CRISPR RNA

(2016) De Dong et al.   NATURE

Programmable editing of a target base in genomic DNA without double-stranded DNA cleavage

(2016) Alexis C. Komor et al.   NATURE

Multiplex gene editing by CRISPR–Cpf1 using a single crRNA array

(2016) Bernd Zetsche et al.   NATURE BIOTECHNOLOGY

Comparison of Cas9 activators in multiple species

(2016) Alejandro Chavez et al.   NATURE METHODS

Morphogenic Regulators Baby boom and Wuschel Improve Monocot Transformation

(2016) Keith Lowe et al.   PLANT CELL

A trait stacking system via intra-genomic homologous recombination

(2016) Sandeep Kumar et al.   PLANTA

Engineering Synthetic Gene Circuits in Living Cells with CRISPR Technology

(2016) Barbara Jusiak et al.   TRENDS IN BIOTECHNOLOGY

Genome editing in maize directed by CRISPR–Cas9 ribonucleoprotein complexes

(2016) Sergei Svitashev et al.   Nature Communications

Efficient and transgene-free genome editing in wheat through transient expression of CRISPR/Cas9 DNA or RNA

(2016) Yi Zhang et al.   Nature Communications

Efficient targeted mutagenesis of rice and tobacco genomes using Cpf1 from Francisella novicida

(2016) Akira Endo et al.   Scientific Reports

Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System

(2015) Bernd Zetsche et al.   CELL

Discovery and Functional Characterization of Diverse Class 2 CRISPR-Cas Systems

(2015) Sergey Shmakov et al.   MOLECULAR CELL

An updated evolutionary classification of CRISPR–Cas systems

(2015) Kira S. Makarova et al.   NATURE REVIEWS MICROBIOLOGY

Nuclear domain ‘knock-in’ screen for the evaluation and identification of small molecule enhancers of CRISPR-based genome editing

(2015) Jordan Pinder et al.   NUCLEIC ACIDS RESEARCH

Biallelic Gene Targeting in Rice

(2015) Masaki Endo et al.   PLANT PHYSIOLOGY

Targeted Mutagenesis, Precise Gene Editing, and Site-Specific Gene Insertion in Maize Using Cas9 and Guide RNA

(2015) Sergei Svitashev et al.   PLANT PHYSIOLOGY

Pharmacological inhibition of DNA-PK stimulates Cas9-mediated genome editing

(2015) Francis Robert et al.   Genome Medicine

DNA Replicons for Plant Genome Engineering

(2014) N. J. Baltes et al.   PLANT CELL

The CRISPR/Cas system can be used as nuclease forin plantagene targeting and as paired nickases for directed mutagenesis in Arabidopsis resulting in heritable progeny

(2014) Simon Schiml et al.   PLANT JOURNAL

Increasing frequencies of site-specific mutagenesis and gene targeting in Arabidopsis by manipulating DNA repair pathways

(2013) Y. Qi et al.   GENOME RESEARCH

Simultaneous generation and germline transmission of multiple gene mutations in rat using CRISPR-Cas systems

(2013) Wei Li et al.   NATURE BIOTECHNOLOGY

In planta gene targeting

(2012) F. Fauser et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

A system for the continuous directed evolution of biomolecules

(2011) Kevin M. Esvelt et al.   NATURE

Genetic Analysis of Zinc-Finger Nuclease-Induced Gene Targeting in Drosophila

(2009) A. Bozas et al.   GENETICS