Review
Cell Biology
Noa Beatriz Martin-Cofreces, Jose Maria Valpuesta, Francisco Sanchez-Madrid
Summary: Lymphocytes undergo shape and organelle rearrangements during interaction with antigen-presenting cells, leading to activation and division through nuclear factors activation. Coordinated enrichment of chaperonin CCT and tubulins at the centrosome area support T cell activation and cytoskeletal dynamics. Proteasome enrichment in activated T cells' centrosome balances protein synthesis and degradation.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Developmental Biology
Ravi Das, Shatabdi Bhattacharjee, Jamin M. Letcher, Jenna M. Harris, Sumit Nanda, Istvan Foldi, Erin N. Lottes, Hansley M. Bobo, Benjamin D. Grantier, Jozsef Mihaly, Giorgio A. Ascoli, Daniel N. Cox
Summary: Dendrite shape is influenced by cytoskeletal fiber organization and dynamics, impacting functional connectivity. Formin 3 is identified as a key regulator of cytoskeletal architecture in nociceptive sensory neurons, playing a crucial role in dendritic arbor complexity. Mutations in the human ortholog of Formin 3 have been linked to Charcot-Marie-Tooth disease, highlighting the importance of understanding cytoskeletal regulation in neuronal function and related neuropathies.
Article
Biology
Mousam Roy, Rachel C. Fleisher, Alexander I. Alexandrov, Amnon Horovitz
Summary: The CCT/TRiC chaperonin is a protein found in the cytosol of all eukaryotic cells that aids in protein folding through ATP-dependent mechanisms. Mutations in subunit CCT2, specifically the T400P and R516H double mutation, have been linked to Leber congenital amaurosis (LCA), a hereditary retinopathy. These mutations also impair the function of CCT2 in promoting autophagy when it is outside of the CCT/TRiC complex.
COMMUNICATIONS BIOLOGY
(2023)
Article
Neurosciences
Brikha R. Shrestha, Anita Burgos, Wesley B. Grueber
Summary: The study reveals the important role of the cell surface molecule Basigin in complex dendrite morphogenesis, with loss of the gene leading to defects in dendrite formation. Knock down of Basigin also affects dendrite elaboration of class IV neurons, suggesting a non-autonomous role in dendrite development.
FRONTIERS IN CELLULAR NEUROSCIENCE
(2021)
Article
Zoology
Jingru Qi, Feng Zhu, Li Shao, Ying Chen, Jicheng Li, Ping He, Ruisha Shang, Fuzhen Sun, Qiang Wang, Yiling Zhang, Xudong Tang, Zhongyuan Shen
Summary: CCT is a molecular chaperone protein widely present in eukaryotic cytoplasm, with NbCCT8 being identified in N. bombycis for the first time. NbCCT8 is expressed throughout the lifecycle of N. bombycis, especially at the early proliferation stage, and may participate in cytoskeleton formation and proliferation of N. bombycis.
JOURNAL OF INVERTEBRATE PATHOLOGY
(2021)
Article
Cell Biology
Amelia Rojas-Gomez, Sara G. Dosil, Francisco J. Chichon, Nieves Fernandez-Gallego, Alessia Ferrarini, Enrique Calvo, Diego Calzada-Fraile, Silvia Requena, Joaquin Oton, Alvaro Serrano, Rocio Tarifa, Montserrat Arroyo, Andrea Sorrentino, Eva Pereiro, Jesus Vazquez, Jose M. Valpuesta, Francisco Sanchez-Madrid, Noa B. Martin-Cofreces
Summary: Cell proteostasis includes various processes such as gene transcription, protein folding, and degradation. By analyzing the proteome of T cell-secreted extracellular vesicles (EVs), researchers have found that the chaperonin complex CCT plays a role in the correct folding of specific proteins. When CCT is limited by siRNA, cells undergo changes in lipid composition and metabolic rewiring towards a lipid-dependent metabolism, resulting in increased activity of peroxisomes and mitochondria. This is due to dysregulation of interorganelle contacts between lipid droplets, mitochondria, peroxisomes, and the endolysosomal system. These findings connect proteostasis with lipid metabolism through an unexpected role of CCT.
JOURNAL OF EXTRACELLULAR VESICLES
(2023)
Article
Biochemistry & Molecular Biology
Abby M. Green, Rachel A. DeWeerd, David R. O'Leary, Ava R. Hansen, Katharina E. Hayer, Katarzyna Kulej, Ariel S. Dineen, Julia H. Szeto, Benjamin A. Garcia, Matthew D. Weitzman
Summary: APOBEC3 enzymes, particularly A3A, are implicated in causing somatic mutations in cancer genomes. A3A interacts with the CCT complex, disrupting CCT function and leading to increased mutational activity. Silencing mutations in CCT subunit genes in cancer genomes are associated with enrichment of A3A mutational signatures.
Article
Multidisciplinary Sciences
Dario Maschi, Ana J. Fernandez-Alvarez, Graciela Lidia Boccaccio
Summary: Proteins from the NANOS family play a crucial role in gonad development and also control neuron maturation and function. Knockdown of Nanos1 in hippocampal neurons impairs synaptogenesis, resulting in smaller and more numerous dendritic spines. Loss of Nanos1 also affects the contact between dendritic PSD95 clusters and pre-synaptic structures, as well as the induction of ARC triggered by neuron depolarization. These findings expand our understanding of the role of NANOS1 in CNS development and suggest its involvement in hippocampal synaptogenesis.
Article
Cell Biology
Julia Popova, Gera A. Pavlova, Alyona Razuvaeva, Lyubov A. Yarinich, Evgeniya N. Andreyeva, Alina F. Anders, Yuliya A. Galimova, Fioranna Renda, Maria Patrizia Somma, Alexey Pindyurin, Maurizio Gatti
Summary: In this study, the process of kinetochore-driven microtubule regrowth (KDMTR) in Drosophila cells was investigated using RNA interference (RNAi). The results showed that several genes are involved in regulating this process, with some genes promoting microtubule polymerization, bundling, and stabilization, while others inhibiting this process.
Article
Multidisciplinary Sciences
Han-Hsuan Liu, Chien-Hsiang Hsu, Lily Y. Jan, Yuh-Nung Jan
Summary: The study established a photo-switchable assay system for detecting dendrite degeneration and repair, and used the WldS protein as a test case to investigate the mechanisms underlying dendrite regeneration and repair.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Multidisciplinary Sciences
Wenyu Han, Mingliang Jin, Caixuan Liu, Qiaoyu Zhao, Shutian Wang, Yifan Wang, Yue Yin, Chao Peng, Yanxing Wang, Yao Cong
Summary: Using cryo-electron microscopy, we investigated the folding mechanism of tubulin/actin under the assistance of the chaperonin TRiC and its co-chaperone plp2. Our findings revealed the interaction between plp2 and tubulin/actin in different chambers of TRiC, and provided insights into the folding mechanism of these cytoskeletal proteins. These findings enhance our understanding of cytoskeletal proteostasis and its relevance to human diseases.
Article
Biology
Caixuan Liu, Mingliang Jin, Shutian Wang, Wenyu Han, Qiaoyu Zhao, Yifan Wang, Cong Xu, Lei Diao, Yue Yin, Chao Peng, Lan Bao, Yanxing Wang, Yao Cong
Summary: This study reveals the conformational landscape of TRiC-mediated tubulin folding along TRiC's ATPase cycle and the interaction sites between tubulin and the closed TRiC chamber using cryo-EM and XL-MS analyses.
COMMUNICATIONS BIOLOGY
(2023)
Article
Biology
Jeffrey D. Hildebrand, Adam D. Leventry, Omoregie P. Aideyman, John C. Majewski, James A. Haddad, Dawn C. Bisi, Nancy Kaufmann
Summary: Regulation of cell architecture is crucial for tissue formation during animal development. Shroom family proteins play a key role in regulating cell morphology by controlling the distribution and activation of Rho-kinase, leading to localized activation of non-muscle myosin II. Understanding the cellular pathways involved in Shroom-mediated changes in cell and tissue morphology is essential for further research in developmental biology.
Article
Neurosciences
Stephanie A. Getz, Kamran Tariq, Dylan H. Marchand, Conor R. Dickson, James R. Howe, Patrick D. Skelton, Wei Wang, Meijie Li, Jeremy M. Barry, Jennifer Hong, Bryan W. Luikart
Summary: PTEN loss leads to dendritic overgrowth in neurons, possibly due to increased microtubule polymerization rate. Inhibiting microtubule polymerization can reduce dendritic overgrowth in Pten KO neurons and improve performance in spatial memory tasks.
JOURNAL OF NEUROSCIENCE
(2022)
Article
Biology
Rohan S. Wakade, Laura C. Ristow, Melanie Wellington, Damian J. Krysan
Summary: Candida albicans, a common human fungal pathogen, undergoes a morphogenetic transition from yeast to filamentous forms during infection. In this study, the authors used intravital imaging assays to screen transcription factor mutants in a mouse model and identified regulators of filament initiation and elongation. They found distinct sets of transcription factors that affect these processes in vivo, including some that have no effect in vitro. Furthermore, they discovered a new mode of function for the widely studied transcription factor Efg1 in mediating relief of Nrg1 repression. This study provides the first characterization of the transcriptional network governing C. albicans filamentation in vivo.
Review
Biochemistry & Molecular Biology
Wei-Kang Yang, Cheng-Ting Chien
Article
Biochemistry & Molecular Biology
Wei-Kang Yang, Yi-Ru Chueh, Ying-Ju Cheng, Dominique Siegenthaler, Jan Pielage, Cheng-Ting Chien
Article
Genetics & Heredity
Pei-Yi Chen, Yi-Wei Tsai, Ying-Ju Cheng, Angela Giangrande, Cheng-Ting Chien
Article
Multidisciplinary Sciences
Yi-Wei Tsai, Hsin-Ho Sung, Jian-Chiuan Li, Chun-Yen Yeh, Pei-Yi Chen, Ying-Ju Cheng, Chun-Hong Chen, Yu-Chen Tsai, Cheng-Ting Chien
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2019)
Article
Biology
Yu-En Lin, Chin-Hsien Lin, En-Peng Ho, Yi-Ci Ke, Stavroula Petridi, Christopher J. H. Elliott, Lee-Yan Sheen, Cheng-Ting Chien
Summary: The water extracts of Gastrodia elata Blume (GE) show neuroprotective effects in G2019S-induced Parkinson's disease fruit flies and transgenic mice by improving motor abilities and protecting dopaminergic neurons. The mechanism involves the activation of the Nrf2 protein in glial cells, which inhibits degenerative signaling pathways in neurons, ultimately preventing neuronal loss and maintaining coordination.
Article
Oncology
Chia-Chen Hsu, Ying-Hsuan Wang, Yi-Yang Chen, Ying-Ju Chen, Chang-Hsien Lu, Yu-Ying Wu, Yao-Ren Yang, Hsing-Yi Tsou, Chian-Pei Li, Cih-En Huang, Chih-Cheng Chen
Summary: Patients with Philadelphia-chromosome-negative myeloproliferative neoplasms (MPNs) are prone to developing second cancers (SCs), which may be attributed to mutations in genes related to immune pathways and inflammatory networks, as well as increased levels of cytokines. This highlights the importance of inflammation in the pathogenesis of MPN-SC.
Review
Biochemistry & Molecular Biology
Ailyn Irvita Ravinther, Hemaniswarri Dewi Dewadas, Shi Ruo Tong, Chai Nien Foo, Yu-En Lin, Cheng-Ting Chien, Yang Mooi Lim
Summary: Parkinson's disease is a common neurodegenerative disease affecting the ageing population, and its prevalence has increased in recent years. Mutations in Leucine-rich-repeat-kinase 2 (LRRK2) are the most common cause of familial Parkinson's disease, and aberrant LRRK2 kinase activity is also associated with idiopathic Parkinson's disease. This review aims to categorize and synthesize current information on LRRK2-linked Parkinson's disease and identify potential therapeutic targets.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell Biology
Hsun Li, Hsin-Ho Sung, Yi-Chun Huang, Ying-Ju Cheng, Hsiao-Fong Yeh, Haiwei Pi, Edward Giniger, Cheng-Ting Chien
Summary: GOPs in the dendrites of Drosophila neurons have been found to play a role in promoting branch extension, but this study reveals their involvement in dendrite retraction as well. The glycosyltransferase Fng localizes increasingly at GOPs and its interaction with Fur2 affects the number of GOPs. Additionally, the Epidermal Delta ligand and neuronal Notch receptor negatively regulate dendrite growth.
Article
Biochemistry & Molecular Biology
Ying-Hsuan Wang, Ying-Ju Chen, Yi-Hua Lai, Ming-Chung Wang, Yi-Yang Chen, Yu-Ying Wu, Yao-Ren Yang, Hsing-Yi Tsou, Chian-Pei Li, Chia-Chen Hsu, Cih-En Huang, Chih-Cheng Chen
Summary: This study found that CALR(DEL) mutation in myeloproliferative neoplasms (MPN) leads to increased expression of S100A8, which promotes cellular proliferation and reduces apoptosis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Hematology
Cih-En Huang, Yi-Yang Chen, Jung -Jung Chang, Yu-Ying Wu, Wei -Ming Chen, Ying-Hsuan Wang, Min-Chi Chen, Chang-Hsien Lu, Chung-Sheng Shi, Chih-Cheng Chen
Summary: This study investigated the associations among Human platelet antigens (HPAs), antiplatelet autoantibodies, and cryoglobulins. The results showed that certain HPA alleles were associated with a low platelet count, the risk of developing ITP, and the presence of antiplatelet antibodies. In HCV-ITP patients, HPA3b was correlated with anti-GPIIb/IIIa antibodies, while in primary ITP patients, HPA3b was correlated with cryoglobulin IgG/A/M.
MEDITERRANEAN JOURNAL OF HEMATOLOGY AND INFECTIOUS DISEASES
(2023)