Article
Multidisciplinary Sciences
Wenyu Han, Mingliang Jin, Caixuan Liu, Qiaoyu Zhao, Shutian Wang, Yifan Wang, Yue Yin, Chao Peng, Yanxing Wang, Yao Cong
Summary: Using cryo-electron microscopy, we investigated the folding mechanism of tubulin/actin under the assistance of the chaperonin TRiC and its co-chaperone plp2. Our findings revealed the interaction between plp2 and tubulin/actin in different chambers of TRiC, and provided insights into the folding mechanism of these cytoskeletal proteins. These findings enhance our understanding of cytoskeletal proteostasis and its relevance to human diseases.
Review
Biochemistry & Molecular Biology
Vikash Kumar, Ankita Behl, Rumaisha Shoaib, Mohammad Abid, Maxim Shevtsov, Shailja Singh
Summary: In this review, we summarize the information on prefoldin family members in archaea and humans, and analyze the unexplored subunits of Plasmodium falciparum (Pf). By comparing with the homologs in archaea and H. sapiens, we enhance the understanding of the functions, structure, and substrate binding mechanism of Pf prefoldin. Our review also highlights the importance of certain PfPFD subunits in malaria parasite biology, as well as their up-regulation during different stages of the parasite life cycle.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2022)
Article
Biochemistry & Molecular Biology
Jorge Cuellar, Josefine Vallin, Andreas Svanstrom, Moises Maestro-Lopez, Barry M. Willardson, Jose M. Valpuesta, Julie Grantham, Maria Teresa Bueno-Carrasco, W. Grant Ludlam
Summary: Gelsolin, an important regulating protein, plays a vital role in actin filament dynamics. During apoptosis, gelsolin is continuously activated due to cleavage. In vitro experiments show that CCT can protect gelsolin from caspase-3 cleavage and form a complex with gelsolin. In cellular experiments, gelsolin and CCT co-localize in regions stimulated by actin dynamics, suggesting that CCT may spatially control actin filament dynamics through localized sequestration of gelsolin.
JOURNAL OF MOLECULAR BIOLOGY
(2022)
Article
Biology
Caixuan Liu, Mingliang Jin, Shutian Wang, Wenyu Han, Qiaoyu Zhao, Yifan Wang, Cong Xu, Lei Diao, Yue Yin, Chao Peng, Lan Bao, Yanxing Wang, Yao Cong
Summary: This study reveals the conformational landscape of TRiC-mediated tubulin folding along TRiC's ATPase cycle and the interaction sites between tubulin and the closed TRiC chamber using cryo-EM and XL-MS analyses.
COMMUNICATIONS BIOLOGY
(2023)
Article
Neurosciences
Chuanyan Yang, Xuezhu Chen, Chenxu Zhang, Xuejiao Lei, Yongling Lu, Yuhai Wang, Hua Feng, Tunan Chen, Yang Yang
Summary: This study aimed to investigate whether the upregulation of acetylated α-tubulin (a-Ac-Tub) could alleviate injury to dendritic spines in the penumbra area and motor dysfunction after ischemic stroke. Knockout of MEC17, the specific acetyltransferase of a-tubulin, in pyramidal neurons exacerbated dendritic spine injury and motor dysfunction after stroke. However, upregulation of MEC17 and treatment with tubastatin A (TBA) maintained mature dendritic spine density and alleviated motor dysfunction after stroke.
CNS NEUROSCIENCE & THERAPEUTICS
(2023)
Article
Biology
Mousam Roy, Rachel C. Fleisher, Alexander I. Alexandrov, Amnon Horovitz
Summary: The CCT/TRiC chaperonin is a protein found in the cytosol of all eukaryotic cells that aids in protein folding through ATP-dependent mechanisms. Mutations in subunit CCT2, specifically the T400P and R516H double mutation, have been linked to Leber congenital amaurosis (LCA), a hereditary retinopathy. These mutations also impair the function of CCT2 in promoting autophagy when it is outside of the CCT/TRiC complex.
COMMUNICATIONS BIOLOGY
(2023)
Review
Neurosciences
Camille Cuveillier, Benoit Boulan, Charlotte Ravanello, Eric Denarier, Jean-Christophe Deloulme, Sylvie Gory-Faure, Christian Delphin, Christophe Bosc, Isabelle Arnal, Annie Andrieux
Summary: MAPs play crucial roles in the development and function of the central nervous system by influencing microtubules and actin cytoskeletons, participating in signaling pathways, and contributing to molecular actions.
FRONTIERS IN MOLECULAR NEUROSCIENCE
(2021)
Article
Cell Biology
Michal Wieczorek, Shih-Chieh Ti, Linas Urnavicius, Kelly R. Molloy, Amol Aher, Brian T. Chait, Tarun M. Kapoor
Summary: The study successfully reconstituted the human gamma-TuRC and generated a subcomplex lacking MZT1 and actin. Comparisons showed that the subcomplex without the lumenal bridge can nucleate microtubules similarly to the holocomplex but with structural differences.
JOURNAL OF CELL BIOLOGY
(2021)
Article
Cell Biology
Justine Maurin, Anne Morel, David Guerit, Julien Cau, Serge Urbach, Anne Blangy, Guillaume Bompard
Summary: Osteoclasts are crucial for bone health, with TUBB6 playing a key role in organizing the cytoskeleton. TUBB6 not only controls microtubule and actin dynamics in osteoclasts, but also regulates other proteins like ARHGAP10, affecting cell activity.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Cell Biology
Yolla German, Loan Vulliard, Anton Kamnev, Laurene Pfajfer, Jakob Huemer, Anna-Katharina Mautner, Aude Rubio, Artem Kalinichenko, Kaan Boztug, Audrey Ferrand, Joerg Menche, Loic Dupre
Summary: The immunological synapse decodes stimulatory signals into adapted lymphocyte responses, and high-content imaging is used to analyze T and NK cells. The study demonstrates how actin cytoskeleton remodeling shapes synapse architecture and affects lytic granule positioning. Analysis of immunodeficient individuals' CD8(+) T cells reveals the roles of ARPC1B and WASP in synapse assembly.
Review
Cell Biology
Tiphaine Douanne, Gillian M. Griffiths
Summary: The immune synapse serves as a crucial site for secretion between immune cells, with mechanisms such as centrosome polarization and rapid actin dynamics playing key roles. Different immune cells utilize a variety of immune synapses to optimize specialized modes of secretion. Research focusing on regulation by centrosomal and actin dynamics in diverse immune cells has been a recent focus.
CURRENT OPINION IN CELL BIOLOGY
(2021)
Article
Biology
Jia C. Wang, Yang-In Yim, Xufeng Wu, Valentin Jaumouille, Andrew Cameron, Clare M. Waterman, John H. Kehrl, John A. Hammer
Summary: This study found that LFA-1:ICAM-1 interactions promote the formation of a contractile actomyosin arc network, which plays a dominant role in B-cell immune synapse (IS) formation. This network is created by mDia1, organized into concentric, contractile arcs by myosin 2A, and flows inward at the same rate as B-cell receptor (BCR):antigen clusters. The results demonstrate that integrin is crucial for synapse formation and inhibiting myosin impairs synapse formation and BCR signaling.
Review
Cell Biology
Abesh Bera, Mohan L. Gupta
Summary: Microtubules are dynamic polymers assembled from alpha- and beta-tubulin subunits, with diverse properties determined by different tubulin isotypes and posttranslational modifications. Research in microorganisms has provided valuable insights into the distinct properties of tubulin isotypes and their roles in facilitating specific cellular processes.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Zoology
Jingru Qi, Feng Zhu, Li Shao, Ying Chen, Jicheng Li, Ping He, Ruisha Shang, Fuzhen Sun, Qiang Wang, Yiling Zhang, Xudong Tang, Zhongyuan Shen
Summary: CCT is a molecular chaperone protein widely present in eukaryotic cytoplasm, with NbCCT8 being identified in N. bombycis for the first time. NbCCT8 is expressed throughout the lifecycle of N. bombycis, especially at the early proliferation stage, and may participate in cytoskeleton formation and proliferation of N. bombycis.
JOURNAL OF INVERTEBRATE PATHOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Hsuan Cheng, Yu-Lin Kao, Ting Chen, Lohitaksh Sharma, Wen-Ting Yang, Yi-Chien Chuang, Shih-Han Huang, Hong-Rui Lin, Yao-Shen Huang, Chi-Ling Kao, Lee-Wei Yang, Rachel Bearon, Hui-Chun Cheng, Kuo-Chiang Hsia, Yu-Chun Lin
Summary: The centrosome, a non-membranous organelle, has a size-dependent diffusion barrier that is regulated by actin filaments and plays a role in protein complex localization.
Article
Cardiac & Cardiovascular Systems
Fernando Alfonso, Jose M. de la Torre Hernandez, Borja Ibanez, Manel Sabate, Manuel Pan, Rajiv Gulati, Jacqueline Saw, Dominick J. Angiolillo, David Adlam, Francisco Sanchez-Madrid
Summary: BA-SCAD is a clinical trial aimed at evaluating the efficacy of pharmacological therapy in patients with SCAD. Patients will be randomly assigned to different treatment methods, and the results will be evaluated based on primary efficacy and safety endpoints. In addition, a comprehensive set of additional substudies will be conducted to gain a holistic understanding of this unique disease.
REVISTA ESPANOLA DE CARDIOLOGIA
(2022)
Article
Immunology
Marta Calvet-Mirabent, Daniel T. Claiborne, Maud Deruaz, Serah Tanno, Carla Serra, Cristina Delgado-Arevalo, Ildefonso Sanchez-Cerrillo, Ignacio de Los Santos, Jesus Sanz, Lucio Garcia-Fraile, Francisco Sanchez-Madrid, Arantzazu Alfranca, Maria Angeles Munoz-Fernandez, Todd M. Allen, Maria J. Buzon, Alejandro Balazs, Vladimir Vrbanac, Enrique Martin-Gayo
Summary: The combination of 2‘3’-c ' diAM(PS)2 and Poly I:C as adjuvants can enhance DCs' ability to induce polyfunctional HIV-1 specific CD8(+) T-cell responses, reducing the severity of CD4(+) T-cell depletion following HIV-1 infection, and preserving the specific polyfunctional responses of CD8(+) T cells. The priming of DCs with PolyI:C and STING agonists may be useful for future HIV-1 vaccine studies.
EUROPEAN JOURNAL OF IMMUNOLOGY
(2022)
Article
Immunology
Laura Esparcia-Pinedo, Pedro Martinez-Fleta, Noelia Ropero, Paula Vera-Tome, Hugh T. Reyburn, Jose M. Casasnovas, Jose M. Rodriguez Frade, Mar Vales-Gomez, Carlos Vilches, Enrique Martin-Gayo, Cecilia Munoz-Calleja, Francisco Sanchez-Madrid, Arantzazu Alfranca
Summary: The immune response to the SARS-CoV-2 vaccination is important for developing new vaccines and optimizing prevention strategies. Analysis of the adaptive immunity in healthy donors and convalescent individuals before and after vaccination revealed specific changes in CD4+ T cell reactivity and antibody levels.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Immunology
Laura Esparcia-Pinedo, Ayla Yarci-Carrion, Gloria Mateo-Jimenez, Noelia Ropero, Laura Gomez-Cabanas, Angel Lancho-Sanchez, Patricia Almendro-Vazquez, Enrique Martin-Gayo, Estela Paz-Artal, Francisco Sanchez-Madrid, Fernando Moldenhauer, Ainhoa Gutierrez-Cobos, Diego Real de Asua, Arantzazu Alfranca
Summary: This study found that individuals with Down syndrome (DS) develop an effective immune response to SARS-CoV-2 vaccination. The cellular and humoral responses to the vaccine were observed in DS individuals after 1 to 3 months and 6 months, with an effective immune response observed in 98% of DS individuals after 6 months.
CLINICAL INFECTIOUS DISEASES
(2023)
Article
Medicine, General & Internal
Marta Calvet-Mirabent, Ildefonso Sanchez Cerillo, Noa Martin-Corfrees, Pedro Martinez-Fleta, Hortensia de la Fuente, Ilya Tsukalov, Cristina Delgado-Arevelo, Maria Jose Calzo, Ignaciode de los Santos, Jesus Sanz, Lucio Garcia-Fraile, Francisco Sanchez Madid, Arantzazu Alfranca, Maria Angles Munoz-Ferandez, Maria J. Buzon, Enrique Martin-Gayo
Summary: This study identifies specific immunometabolic parameters associated with functional restoration of CD8+ T cells in people living with HIV-1 on long-term ART. The findings suggest that CD8+ T cells from these patients show improved polyfunctionality and capacity to eliminate infected CD4+ T cells after treatment with Gag-adjuvant-primed DC. However, CD8+ T cells from patients on short-term ART show limited functional improvement after DC treatment, which is associated with altered memory cell subsets, increased expression of PD1 and TIGIT, and reduced metabolic activity upon TCR activation. Restoring the function of CD8+ T cells from these patients can be achieved through combined treatment with anti-PD1 and anti-TIGIT antibodies and a glycolysis promoting drug.
Article
Chemistry, Multidisciplinary
Antonella Russo, Hendrik Schuermann, Matthias Brandt, Katja Scholz, Anna Livia L. Matos, David Grill, Julian Revenstorff, Max Rembrink, Meike von Wulffen, Lena Fischer-Riepe, Peter J. Hanley, Hans Haecker, Monika Pruenster, Francisco Sanchez-Madrid, Sven Hermann, Luisa Klotz, Volker Gerke, Timo Betz, Thomas Vogl, Johannes Roth
Summary: It has been demonstrated that S100A8/S100A9 tetramers can weaken the inflammatory response of leukocytes in vitro and in vivo, and this is mediated by the interaction between S100A8/S100A9 tetramers and CD69, rather than the binding of S100A8/S100A9 dimers to TLR4. These findings reveal the important role of the quaternary structure of S100 proteins in regulating leukocyte inflammatory responses.
Article
Immunology
Raul Izquierdo-Serrano, Irene Fernandez-Delgado, Olga Moreno-Gonzalo, Enrique Martin-Gayo, Diego Calzada-Fraile, Marta Ramirez-Huesca, Inmaculada Jorge, Emilio Camafeita, Joaquin Abian, Miguel Vicente-Manzanares, Esteban Veiga, Jesus Vazquez, Francisco Sanchez-Madrid
Summary: Communication through cell-cell contacts and extracellular vesicles allows immune cells to coordinate their responses against pathogens. The proteins and nucleic acids loaded into extracellular vesicles play a crucial role in this process. Acetylation, controlled by HDAC6, is a mechanism of cellular retention during protein sorting to exosomes. During infection, protein content of dendritic cells and their secreted extracellular vesicles change and become enriched in antiviral proteins. These extracellular vesicles can induce anti-pathogenic gene expression in recipient cells, leading to protection against subsequent viral infection.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Medicine, Research & Experimental
Cristina Delgado-Arevalo, Marta Calvet-Mirabent, Ana Triguero-Martinez, Enrique Vazquez de Luis, Alberto Benguria-Filippini, Raquel Largo, Diego Calzada-Fraile, Olga Popova, Ildefonso Sanchez-Cerrillo, Ilya Tsukalov, Roberto Moreno-Vellisca, Hortensia de la Fuente, Gabriel Herrero-Beaumont, Almudena Ramiro, Francisco Sanchez-Madrid, Santos Castaneda, Ana Dopazo, Isidoro Gonzalez alvaro, Enrique Martin-Gayo
Summary: This study assessed the characteristics of CD1c+ and CD141+ cDC and monocytes in patients with RA, revealing the association between the presence of CD1c+ cDC in the synovial membrane and the higher expression of CCR2 and CD64. Additionally, synovial CD1c+ cDC exhibited increased proinflammatory cytokine expression and the ability to induce pathogenic T cells. The crosstalk between Fcγ receptors and NLRC4 was identified as a potential mechanism mediating the pathogenic activation, CD64 upregulation, and functional specialization of CD1c+ cDC in response to dsDNA-IgG in patients with RA.
Article
Multidisciplinary Sciences
Antonio Bouthelier, Lucia Fernandez-Arroyo, Claudia Mesa-Ciller, Danay Cibrian, Noa Beatriz Martin-Cofreces, Raquel Castillo-Gonzalez, Macarena Calero, Diego Herraez-Aguilar, Andrea Guajardo-Grence, Ana Maria Pacheco, Ana Marcos-Jimenez, Borja Quiroga, Marta Morado, Francisco Monroy, Cecilia Munoz-Calleja, Francisco Sanchez-Madrid, Andres A. Urrutia, Julian Aragones
Summary: Inhibition of the amino acid carrier SLC7A5/SLC3A2 has been extensively studied in tumor biology, but its role in physiological conditions is not well understood. This study demonstrates that the SLC7A5/SLC3A2 heterodimer is present during erythroid differentiation but absent in mature red blood cells. Erythropoietin induces expression of SLC7A5/SLC3A2 in reticulocytes, and its deficiency leads to reduced size and hemoglobin content in circulating red blood cells. These findings highlight the importance of SLC7A5/SLC3A2 in reticulocyte maturation and maintaining proper characteristics of red blood cells.
Article
Cell Biology
Amelia Rojas-Gomez, Sara G. Dosil, Francisco J. Chichon, Nieves Fernandez-Gallego, Alessia Ferrarini, Enrique Calvo, Diego Calzada-Fraile, Silvia Requena, Joaquin Oton, Alvaro Serrano, Rocio Tarifa, Montserrat Arroyo, Andrea Sorrentino, Eva Pereiro, Jesus Vazquez, Jose M. Valpuesta, Francisco Sanchez-Madrid, Noa B. Martin-Cofreces
Summary: Cell proteostasis includes various processes such as gene transcription, protein folding, and degradation. By analyzing the proteome of T cell-secreted extracellular vesicles (EVs), researchers have found that the chaperonin complex CCT plays a role in the correct folding of specific proteins. When CCT is limited by siRNA, cells undergo changes in lipid composition and metabolic rewiring towards a lipid-dependent metabolism, resulting in increased activity of peroxisomes and mitochondria. This is due to dysregulation of interorganelle contacts between lipid droplets, mitochondria, peroxisomes, and the endolysosomal system. These findings connect proteostasis with lipid metabolism through an unexpected role of CCT.
JOURNAL OF EXTRACELLULAR VESICLES
(2023)
Article
Biochemical Research Methods
Andrea Laguillo-Gomez, Enrique Calvo, Noa Martin-Cofreces, Marta Lozano-Prieto, Francisco Sanchez-Madrid, Jesus Vazquez
Summary: Open-search methods with prior knowledge improve identification performance of protein post-translational modifications by minimizing experimental errors and precursor mass assignation. The introduction of a novel approach enables the study of a wider variety of PTMs, including unknown or unexpected modifications.
JOURNAL OF PROTEOMICS
(2023)
Article
Multidisciplinary Sciences
Diego Calzada-Fraile, Salvador Iborra, Marta Ramirez-Huesca, Inmaculada Jorge, Enrico Dotta, Elena Hernandez-Garcia, Noa Martin-Cofreces, Estanislao Nistal-Villan, Esteban Veiga, Jesus Vazquez, Giulia Pasqual, Francisco Sanchez-Madrid
Summary: Antigen presentation induces upregulation of MHC-I protein molecules and increased lipid peroxidation on psDCs, mediating DC licensing. Adoptive transfer of psDC enhances pathogen-specific CD8(+) T cell responses and protects mice from infection. The study reveals crucial molecular events underlying psDC licensing.
NATURE COMMUNICATIONS
(2023)
Article
Immunology
Alvaro Gomez-Moron, Silvia Requena, Clara Pertusa, Marta Lozano-Prieto, Diego Calzada-Fraile, Camila Scagnetti, Ines Sanchez-Garcia, Ana Adela Calero-Garcia, Manuel Izquierdo, Noa B. Martin-Cofreces
Summary: The organization of the mitochondrial network is crucial for T cell metabolism and response to TCR stimulation. This study focuses on the role of EB1 in regulating intracellular organization and metabolic strength in activated T cells, revealing the connection between cytoskeleton dynamics and metabolism in TCR-mediated processes such as AICD. By elucidating the relationship between tubulin cytoskeleton and mitochondria, this work emphasizes the importance of this connection for critical cell functions.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Medicine, Research & Experimental
Rafael Blanco-Dominguez, Hortensia de la Fuente, Cristina Rodriguez, Laura Martin-Aguado, Raquel Sanchez-Diaz, Rosa Jimenez-Alejandre, Iker Rodriguez-Arabaolaza, Andrea Curtabbi, Marcos M. Garcia-Guimaraes, Alberto Vera, Fernando Rivero, Javier Cuesta, Luis J. Jimenez-Borreguero, Alberto Cecconi, Albert Duran-Cambra, Manel Tauron, Judith Alonso, Hector Bueno, Maria Villalba-Orero, Jose Antonio Enriquez, Simon C. Robson, Fernando Alfonso, Francisco Sanchez-Madrid, Jose Martinez-Gonzalez, Pilar Martin
Summary: Increasing CD69 expression on T cells after myocardial infarction may play an important role in controlling immune response and improving cardiac function. Clinical data suggests that increased CD69 expression is associated with a lower risk of heart failure.
JOURNAL OF CLINICAL INVESTIGATION
(2022)
Article
Medicine, General & Internal
Emilia Roy-Vallejo, Laura Cardenoso, Ana Triguero-Martinez, Marta Chicot Llano, Nelly Zurita, Elena Avalos, Ana Barrios, Julia Hernando, Javier Ortiz, Sebastian C. Rodriguez-Garcia, Marianela Ciudad Sanudo, Celeste Marcos, Elena Garcia Castillo, Leticia Fontan Garcia-Rodrigo, Begona Gonzalez, Rosa Mendez, Isabel Iturrate, Ancor Sanz-Garcia, Almudena Villa, Ana Sanchez-Azofra, Begona Quicios, David Arribas, Jesus Alvarez Rodriguez, Pablo Patino, Marina Trigueros, Miren Uriarte, Alexandra Martin-Ramirez, Cristina Arevalo Roman, Jose Maria Galvan-Roman, Rosario Garcia-Vicuna, Julio Ancochea, Cecilia Munoz-Calleja, Elena Fernandez-Ruiz, Rafael de la Camara, Carmen Suarez Fernandez, Isidoro Gonzalez-Alvaro, Diego A. Rodriguez-Serrano, PREDINMUN COVID Grp
Summary: This study aimed to evaluate the relationship between SARS-CoV-2 viral load and IL6 levels in COVID-19 patients. The results showed that in patients with worse outcomes, there was an early peak of SARS-CoV-2 viral load followed by an increase in IL6 levels. Monitoring viral load during the first week after symptom onset may help predict disease severity in COVID-19 patients.
FRONTIERS IN MEDICINE
(2022)