Modulation of DNA double-strand break repair as a strategy to improve precise genome editing

Tandem Paired Nicking Promotes Precise Genome Editing with Scarce Interference by p53

(2020) Toshinori Hyodo et al.   Cell Reports

Identification and characterization of novel SCR7‐based small‐molecule inhibitor of DNA end‐joining, SCR130 and its relevance in cancer therapeutics

(2020) Ujjayinee Ray et al.   MOLECULAR CARCINOGENESIS

Timed inhibition of CDC7 increases CRISPR-Cas9 mediated templated repair

(2020) Beeke Wienert et al.   Nature Communications

CAS9 is a genome mutator by directly disrupting DNA-PK dependent DNA repair pathway

(2020) Shuxiang Xu et al.   Protein & Cell

Suppressing the NHEJ pathway by DNA-PKcs inhibitor NU7026 prevents degradation of HBV cccDNA cleaved by CRISPR/Cas9

(2019) Dmitry Kostyushev et al.   Scientific Reports

Enhancement of Precise Gene Editing by the Association of Cas9 With Homologous Recombination Factors

(2019) Ngoc-Tung Tran et al.   Frontiers in Genetics

Stimulation of CRISPR-mediated homology-directed repair by an engineered RAD18 variant

(2019) Tarun S. Nambiar et al.   Nature Communications

CRISPR-Cas9 fusion to dominant-negative 53BP1 enhances HDR and inhibits NHEJ specifically at Cas9 target sites

(2019) Rajeswari Jayavaradhan et al.   Nature Communications

CRISPR-READI: Efficient Generation of Knockin Mice by CRISPR RNP Electroporation and AAV Donor Infection

(2019) Sean Chen et al.   Cell Reports

Simultaneous precise editing of multiple genes in human cells

(2019) Stephan Riesenberg et al.   NUCLEIC ACIDS RESEARCH

Kinetics and Fidelity of the Repair of Cas9-Induced Double-Strand DNA Breaks

(2018) Eva K. Brinkman et al.   MOLECULAR CELL

MRI Is a DNA Damage Response Adaptor during Classical Non-homologous End Joining

(2018) Putzer J. Hung et al.   MOLECULAR CELL

CRISPR–Cas9 genome editing in human cells occurs via the Fanconi anemia pathway

(2018) Chris D. Richardson et al.   NATURE GENETICS

MRN complex-dependent recruitment of ubiquitylated BLM helicase to DSBs negatively regulates DNA repair pathways

(2018) Vivek Tripathi et al.   Nature Communications

Targeting repair pathways with small molecules increases precise genome editing in pluripotent stem cells

(2018) Stephan Riesenberg et al.   Nature Communications

Ligase IV inhibitor SCR7 enhances gene editing directed by CRISPR–Cas9 and ssODN in human cancer cells

(2018) Zheng Hu et al.   Cell and Bioscience

Covalent linkage of the DNA repair template to the CRISPR-Cas9 nuclease enhances homology-directed repair

(2018) Natasa Savic et al.   eLife

Time Matters: Gene Editing at the Mouse 2-Cell Embryo Stage Boosts Knockin Efficiency

(2018) Alvaro Plaza Reyes et al.   Cell Stem Cell

Autocyclized and Oxidized Forms of SCR7 Induce Cancer Cell Death by Inhibiting Nonhomologous DNA End joining in a Ligase IV Dependent Manner

(2018) Supriya V. Vartak et al.   FEBS Journal

Programmable DNA repair with CRISPRa/i enhanced homology-directed repair efficiency with a single Cas9

(2018) Lupeng Ye et al.   Cell Discovery

CRISPR/Cas9-mediated homology-directed repair by ssODNs in zebrafish induces complex mutational patterns resulting from genomic integration of repair-template fragments

(2018) Annekatrien Boel et al.   Disease Models & Mechanisms

Gene Editing of HIV-1 Co-receptors to Prevent and/or Cure Virus Infection

(2018) Alexander G. Allen et al.   Frontiers in Microbiology

Enhancing CRISPR/Cas9-mediated homology-directed repair in mammalian cells by expressing Saccharomyces cerevisiae Rad52

(2017) Simin Shao et al.   INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY

A CRISPR/Cas9–Based Screening for Non-Homologous End Joining Inhibitors Reveals Ouabain and Penfluridol as Radiosensitizers

(2017) Jie Du et al.   MOLECULAR CANCER THERAPEUTICS

Inhibition of 53BP1 favors homology-dependent DNA repair and increases CRISPR–Cas9 genome-editing efficiency

(2017) Marella D Canny et al.   NATURE BIOTECHNOLOGY

Easi-CRISPR for creating knock-in and conditional knockout mouse models using long ssDNA donors

(2017) Hiromi Miura et al.   Nature Protocols

Assembly of CRISPR ribonucleoproteins with biotinylated oligonucleotides via an RNA aptamer for precise gene editing

(2017) Jared Carlson-Stevermer et al.   Nature Communications

Inactivation of Pol θ and C-NHEJ eliminates off-target integration of exogenous DNA

(2017) Alex N. Zelensky et al.   Nature Communications

Disruption of diphthamide synthesis genes and resulting toxin resistance as a robust technology for quantifying and optimizing CRISPR/Cas9-mediated gene editing

(2017) Tobias Killian et al.   Scientific Reports

Optimizing the DNA Donor Template for Homology-Directed Repair of Double-Strand Breaks

(2017) Fei Song et al.   Molecular Therapy-Nucleic Acids

Ectopic expression of RAD52 and dn53BP1 improves homology-directed repair during CRISPR–Cas9 genome editing

(2017) Bruna S. Paulsen et al.   Nature Biomedical Engineering

A Life Investigating Pathways That Repair Broken Chromosomes

(2016) James E. Haber   Annual Review of Genetics

High-Throughput, High-Resolution Mapping of Protein Localization in Mammalian Brain by In Vivo Genome Editing

(2016) Takayasu Mikuni et al.   CELL

SCR7 is neither a selective nor a potent inhibitor of human DNA ligase IV

(2016) George E. Greco et al.   DNA REPAIR

The democratization of gene editing: Insights from site-specific cleavage and double-strand break repair

(2016) Maria Jasin et al.   DNA REPAIR

Origins of Programmable Nucleases for Genome Engineering

(2016) Srinivasan Chandrasegaran et al.   JOURNAL OF MOLECULAR BIOLOGY

In vivo genome editing via CRISPR/Cas9 mediated homology-independent targeted integration

(2016) Keiichiro Suzuki et al.   NATURE

Applications of CRISPR technologies in research and beyond

(2016) Rodolphe Barrangou et al.   NATURE BIOTECHNOLOGY

53BP1 fosters fidelity of homology-directed DNA repair

(2016) Fena Ochs et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Chromosome territory relocation during DNA repair requires nuclear myosin 1 recruitment to chromatin mediated by ϒ-H2AX signaling

(2016) Mugdha Kulashreshtha et al.   NUCLEIC ACIDS RESEARCH

Bridging of double-stranded breaks by the nonhomologous end-joining ligation complex is modulated by DNA end chemistry

(2016) Dylan A. Reid et al.   NUCLEIC ACIDS RESEARCH

Increasing the efficiency of CRISPR/Cas9-mediated precise genome editing in rats by inhibiting NHEJ and using Cas9 protein

(2016) Yuanwu Ma et al.   RNA Biology

Advances in therapeutic CRISPR/Cas9 genome editing

(2016) Nataša Savić et al.   Translational Research

Repair Pathway Choices and Consequences at the Double-Strand Break

(2016) Raphael Ceccaldi et al.   TRENDS IN CELL BIOLOGY

Regulation of Single-Strand Annealing and its Role in Genome Maintenance

(2016) Ragini Bhargava et al.   TRENDS IN GENETICS

Genome-Editing Technologies: Principles and Applications

(2016) Thomas Gaj et al.   Cold Spring Harbor Perspectives in Biology

53BP1 fosters fidelity of homology-directed DNA repair

(2016) Fena Ochs et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Two Distinct Pathways Support Gene Correction by Single-Stranded Donors at DNA Nicks

(2016) Luther Davis et al.   Cell Reports

Post-translational Regulation of Cas9 during G1 Enhances Homology-Directed Repair

(2016) Tony Gutschner et al.   Cell Reports

High-Resolution CRISPR Screens Reveal Fitness Genes and Genotype-Specific Cancer Liabilities

(2015) Traver Hart et al.   CELL

Small Molecules Enhance CRISPR Genome Editing in Pluripotent Stem Cells

(2015) Chen Yu et al.   Cell Stem Cell

DNA Double-Strand Break Repair Inhibitors as Cancer Therapeutics

(2015) Mrinal Srivastava et al.   CHEMISTRY & BIOLOGY

Pluronic copolymer encapsulated SCR7 as a potential anticancer agent

(2015) Franklin John et al.   FARADAY DISCUSSIONS

Snaps and mends: DNA breaks and chromosomal translocations

(2015) Saniya M. Javadekar et al.   FEBS Journal

Efficient and Allele-Specific Genome Editing of Disease Loci in Human iPSCs

(2015) Cory Smith et al.   MOLECULAR THERAPY

A mechanism for the suppression of homologous recombination in G1 cells

(2015) Alexandre Orthwein et al.   NATURE

Increasing the efficiency of precise genome editing with CRISPR-Cas9 by inhibition of nonhomologous end joining

(2015) Takeshi Maruyama et al.   NATURE BIOTECHNOLOGY

Increasing the efficiency of homology-directed repair for CRISPR-Cas9-induced precise gene editing in mammalian cells

(2015) Van Trung Chu et al.   NATURE BIOTECHNOLOGY

Small molecule–triggered Cas9 protein with improved genome-editing specificity

(2015) Kevin M Davis et al.   Nature Chemical Biology

Mechanism of microhomology-mediated end-joining promoted by human DNA polymerase θ

(2015) Tatiana Kent et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Nuclear domain ‘knock-in’ screen for the evaluation and identification of small molecule enhancers of CRISPR-based genome editing

(2015) Jordan Pinder et al.   NUCLEIC ACIDS RESEARCH

Organization and dynamics of the nonhomologous end-joining machinery during DNA double-strand break repair

(2015) Dylan A. Reid et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

PAXX, a paralog of XRCC4 and XLF, interacts with Ku to promote DNA double-strand break repair

(2015) T. Ochi et al.   SCIENCE

Production of Gene-Corrected Adult Beta Globin Protein in Human Erythrocytes Differentiated from Patient iPSCs After Genome Editing of the Sickle Point Mutation

(2015) Xiaosong Huang et al.   STEM CELLS

Improved Hematopoietic Differentiation Efficiency of Gene-Corrected Beta-Thalassemia Induced Pluripotent Stem Cells by CRISPR/Cas9 System

(2015) Bing Song et al.   STEM CELLS AND DEVELOPMENT

Regulation of Recombination and Genomic Maintenance

(2015) Wolf-Dietrich Heyer   Cold Spring Harbor Perspectives in Biology

A generic strategy for CRISPR-Cas9-mediated gene tagging

(2015) Daniel H. Lackner et al.   Nature Communications

Mechanism of microhomology-mediated end-joining promoted by human DNA polymerase θ

(2015) Tatiana Kent et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Pluronic copolymer encapsulated SCR7 as a potential anticancer agent

(2015) Franklin John et al.   FARADAY DISCUSSIONS

Pharmacological inhibition of DNA-PK stimulates Cas9-mediated genome editing

(2015) Francis Robert et al.   Genome Medicine

Preventing Damage Limitation: Targeting DNA-PKcs and DNA Double-Strand Break Repair Pathways for Ovarian Cancer Therapy

(2015) Daniela A. Dungl et al.   Frontiers in Oncology

A Protein-Tagging System for Signal Amplification in Gene Expression and Fluorescence Imaging

(2014) Marvin E. Tanenbaum et al.   CELL

Development and Applications of CRISPR-Cas9 for Genome Engineering

(2014) Patrick D. Hsu et al.   CELL

A Mouse Geneticist's Practical Guide to CRISPR Applications

(2014) P. Singh et al.   GENETICS

Enhanced Efficacy of Pluronic Copolymer Micelle Encapsulated SCR7 against Cancer Cell Proliferation

(2014) Franklin John et al.   MACROMOLECULAR BIOSCIENCE

Efficient genome modification by CRISPR-Cas9 nickase with minimal off-target effects

(2014) Bin Shen et al.   NATURE METHODS

Phosphorylation of EXO1 by CDKs 1 and 2 regulates DNA end resection and repair pathway choice

(2014) Nozomi Tomimatsu et al.   Nature Communications

One-Step Generation of Mice Carrying Mutations in Multiple Genes by CRISPR/Cas-Mediated Genome Engineering

(2013) Haoyi Wang et al.   CELL

One-Step Generation of Mice Carrying Reporter and Conditional Alleles by CRISPR/Cas-Mediated Genome Engineering

(2013) Hui Yang et al.   CELL

Double Nicking by RNA-Guided CRISPR Cas9 for Enhanced Genome Editing Specificity

(2013) F. Ann Ran et al.   CELL

Highly efficient CRISPR/Cas9-mediated knock-in in zebrafish by homology-independent DNA repair

(2013) T. O. Auer et al.   GENOME RESEARCH

RNA-Guided Human Genome Engineering via Cas9

(2013) P. Mali et al.   SCIENCE

An Inhibitor of Nonhomologous End-Joining Abrogates Double-Strand Break Repair and Impedes Cancer Progression

(2012) Mrinal Srivastava et al.   CELL

APLF promotes the assembly and activity of non-homologous end joining protein complexes

(2012) Gabrielle J Grundy et al.   EMBO JOURNAL

RAD52 inactivation is synthetically lethal with deficiencies in BRCA1 and PALB2 in addition to BRCA2 through RAD51-mediated homologous recombination

(2012) B H Lok et al.   ONCOGENE

A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity

(2012) M. Jinek et al.   SCIENCE

Over-expression of RAD51 or RAD54 but not RAD51/4 enhances extra-chromosomal homologous recombination in the human sarcoma (HT-1080) cell line

(2011) Shengli Yu et al.   JOURNAL OF BIOTECHNOLOGY

How does DNA break during chromosomal translocations?

(2011) M. Nambiar et al.   NUCLEIC ACIDS RESEARCH

The Mechanism of Double-Strand DNA Break Repair by the Nonhomologous DNA End-Joining Pathway

(2010) Michael R. Lieber   Annual Review of Biochemistry

Targeting DNA Double-Strand Breaks with TAL Effector Nucleases

(2010) M. Christian et al.   GENETICS

Human hematopoietic stem/progenitor cells modified by zinc-finger nucleases targeted to CCR5 control HIV-1 in vivo

(2010) Nathalia Holt et al.   NATURE BIOTECHNOLOGY

Alternative end-joining catalyzes robust IgH locus deletions and translocations in the combined absence of ligase 4 and Ku70

(2010) C. Boboila et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Breaking the Code of DNA Binding Specificity of TAL-Type III Effectors

(2009) J. Boch et al.   SCIENCE

A Simple Cipher Governs DNA Recognition by TAL Effectors

(2009) M. J. Moscou et al.   SCIENCE

Comparison of nonhomologous end joining and homologous recombination in human cells

(2008) Z MAO et al.   DNA REPAIR

Rad51 Protein Controls Rad52-mediated DNA Annealing

(2008) Yun Wu et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Efficient gene targeting in Drosophila by direct embryo injection with zinc-finger nucleases

(2008) K. J. Beumer et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA