Article
Chemistry, Analytical
Chenghong Lei, Dehong Hu
Summary: By synchronizing electrochemical potential scanning with a single-molecule localization super-resolution fluorescence microscope, this study tracked kinetic fluorescence changes of hundreds of single molecular redox events simultaneously with high throughput. Through subsequent cross-correlation function analysis, the redox potentials of single molecules were mapped out in unprecedented detail from site to site in the imaging area. This work lays the foundation for mapping redox states at single-molecule levels in high throughput in chemical and biological systems.
ANALYTICAL CHEMISTRY
(2021)
Review
Chemistry, Multidisciplinary
Irena Trbojevic-Akmaciic, Guinevere S. M. Lageveen-Kammeijer, Bram Heijs, Tea Petrovic, Helena Deris, Manfred Wuhrer, Gordan Lauc
Summary: Glycomics is a discipline that aims to understand the structure, function, and regulation of glycans produced in cells or organisms. High-throughput glycomics, which involves analyzing hundreds or thousands of glycomes in a timely manner, requires robust and sensitive analytical technologies. This review provides a comprehensive overview of the history, recent developments, and challenges of high-throughput glycomics, with a focus on N-glycosylation analysis.
Article
Environmental Sciences
Susana I. L. Gomes, Tjalf E. de Boer, Cornelis A. M. van Gestel, Nico M. van Straalen, Amadeu M. V. M. Soares, Dick Roelofs, Monica J. B. Amorim
Summary: This study revealed the molecular mechanisms of zinc toxicity in Enchytraeus crypticus, including zinc trafficking, oxidative stress, and effects on the nervous system. Gene expression changes can predict the adverse effects of zinc at the organism level.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Multidisciplinary Sciences
Arthur Viode, Patrick van Zalm, Kinga K. Smolen, Benoit Fatou, David Stevenson, Meenakshi Jha, Ofer Levy, Judith Steen, Hanno Steen
Summary: We present a cost-effective, robust high-throughput-compatible method for depleting plasma, enabling comprehensive profiling of plasma with detection of >1300 proteins per run and throughput of 60 samples per day. The method has been extensively validated, processing >3000 samples with no apparent batch effect, and costs around $2.5 per sample for the depletion step.
Article
Chemistry, Analytical
Roshan Aggarwal, Bryn Ferris, Huiyan Li
Summary: A new microarray format, named compartmentalized linker array (CLA), was proposed, which consists of pre-prepared microarrays of chemical linkers in microliter compartments. CLA can bind and pattern bioreagents into microarrays by simply pipetting and incubating bioreagent solutions in compartments. The application of CLA was demonstrated for a multiplexed sandwich immunoassay measuring three cancer-related proteins.
ANALYTICAL CHEMISTRY
(2023)
Article
Biology
Peter Lonn, Rasel A. Al-Amin, Ehsan Manouchehri Doulabi, Johan Heldin, Radiosa Gallini, Johan Bjorkesten, Johan Oelrich, Masood Kamali-Moghaddam, Ulf Landegren
Summary: The study developed a semi-automated system for large-scale in situ proximity ligation assays (isPLA), expanding the scope for image-based single cell analyses by combining observations of protein interactions and modifications with morphological details of individual cells at high throughput.
COMMUNICATIONS BIOLOGY
(2021)
Article
Chemistry, Analytical
Andrew P. Burns, Ya-Qin Zhang, Tuan Xu, Zhengxi Wei, Qin Yao, Yuhong Fang, Valeriu Cebotaru, Menghang Xia, Matthew D. Hall, Ruili Huang, Anton Simeonov, Christopher A. LeClair, Dingyin Tao
Summary: The development of a highly automated universal sample preparation platform has significantly improved the efficiency of proteomics sample preparation, enabling high-throughput extraction and analysis of proteins from various human cell types. This platform demonstrates excellent digestion efficiency, reproducibility, and universality in quantifying target proteins, showcasing its potential for clinical research.
ANALYTICAL CHEMISTRY
(2021)
Article
Immunology
Hantao Lou, Guang Sheng Ling, Xuetao Cao
Summary: Systemic lupus erythematosus (SLE) is an autoimmune disease characterized by organ damage and a wide range of autoantibodies. The mechanisms of tissue damage and inflammation caused by autoantibodies are not fully understood, but studying autoreactive B cells and pathogenic autoantibodies can enhance our understanding of the origins and targets of SLE. Developing specific therapies targeting pathogenic B cell subsets and autoantibodies remains a challenge. Comprehensive autoantibody profiling and functional characterization can provide new insights into the etiology and pathogenesis of SLE.
JOURNAL OF AUTOIMMUNITY
(2022)
Review
Biochemical Research Methods
Joyita Sarkar, Ashok Kumar
Summary: High-throughput systems are widely used in various fields such as bioprocess industries, pollutant detection, and material microarrays. The application of biomaterials in these systems has facilitated cell-based assays and the discovery of novel biomaterials. However, challenges in clinical applicability still exist, requiring focus on analysis techniques and storage/shelf-life of cell-laden microarrays.
BIOTECHNOLOGY JOURNAL
(2021)
Review
Biochemical Research Methods
Sunil Shrestha, Vinod Kumar Reddy Lekkala, Prabha Acharya, Darshita Siddhpura, Moo-Yeal Lee
Summary: This article discusses the traditional methods of spheroid and multicellular tissue cultures in 3D cell culture, as well as the technical challenges involved. It also introduces the use of droplet-based, miniature 3D bioprinting to enhance assay throughput and reproducibility for high-throughput, predictive screening of compounds. Various platforms for miniature spheroid and tissue cultures via microarray 3D bioprinting are presented.
Article
Chemistry, Multidisciplinary
Yuehai Mei, Wenmao Huang, Weishuai Di, Xin Wang, Zhenshu Zhu, Yanyan Zhou, Fengwei Huo, Wei Wang, Yi Cao
Summary: The study proposes a mechanochemical lithography (MCL) method based on compressive force-triggered reactions for creating surfaces with patterned biomolecules. The method allows printing in physiological conditions and enables the creation of complex structures. The researchers discovered that amino groups in biomolecules can react with maleimide through a force-triggered reaction, resulting in mechanically and chemically stable covalent linkages.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2022)
Article
Multidisciplinary Sciences
Stefan Kraemer, Andreas Moritz, Luca Stehl, Meike Hutt, Martin Hofmann, Claudia Wagner, Sebastian Bunk, Dominik Maurer, Guenter Roth, Johannes Woehrle
Summary: By combining stabilized peptide-receptive HLA molecules with microarray printing and screening, an ultra-high-throughput screening platform named ValidaTe has been developed for large scale evaluation of pHLA-binder interactions. Compared to conventional bio-layer interferometry measurements, the microarray screening achieved a massively increased throughput (over 650 fold), paving the way for pre-clinical safety screening of pHLA-targeting drugs.
SCIENTIFIC REPORTS
(2023)
Review
Chemistry, Multidisciplinary
Yifeng Chen, Cong Wang, Kefeng Ren, Jian Ji
Summary: High-throughput screening has become increasingly important in various fields for accelerating the material development process, with droplet microarrays as an emerging high-throughput platform that offers advantages such as low reagent and sample consumption, short reaction time, high integration, and strong operability in biomedical research.
PROGRESS IN CHEMISTRY
(2021)
Article
Oncology
Vanesa Biolatti, Lara Negrin, Nicolas Bellora, Irene L. Ibanez
Summary: The study identified potential predictive biomarkers for biodosimetry and radioinduced-response through high-throughput gene expression analysis in IR-exposed human peripheral white blood cells. Six selected DEGs were validated by qRT-PCR, showing significant upregulation post IR-exposure, with PCNA expression levels increasing dose-dependently. Notably, TCF4 was bioinformatically detected as an overrepresented TF in the radioinduced DEGs set and was validated as an IR-responsive gene post-irradiation.
RADIOTHERAPY AND ONCOLOGY
(2021)
Article
Chemistry, Analytical
Brock R. Juliano, Joseph W. Keating, Henry W. Li, Anna G. Anders, Zhuoer Xie, Brandon T. Ruotolo
Summary: Native ion mobility mass spectrometry (nIM-MS) combined with collision-induced unfolding (CIU) experiment is an effective technique for rapid evaluation of biomolecular structures and stability. In this study, a RapidFire robotic sample handling system was used to develop an automated, high-throughput methodology for nMS and CIU, called nRapidFire-MS. The results show that nRapidFire-MS can accurately track the same conformational populations as standard nMS and CIU approaches, and has the potential for high-throughput analysis of biopharmaceutical samples.
ANALYTICAL CHEMISTRY
(2023)
Article
Rheumatology
Margaret H. Y. Ma, Sen Hee Tay, Peter P. M. Cheung, Amelia Santosa, Yiong Huak Chan, James W. L. Yip, Anselm Mak, Manjari Lahiri
Summary: The study identified three distinct clusters of patients with rheumatic diseases during the COVID-19 pandemic, each showing different healthcare beliefs and sociodemographics. Patients in Cluster 3 were most concerned about COVID-19, more likely to wear face masks, and more likely to alter or stop their medications.
JOURNAL OF RHEUMATOLOGY
(2021)
Review
Rheumatology
Weiqin Lin, Sen Hee Tay, Anselm Mak
Summary: Takotsubo syndrome (TTS) is an acute cardiac condition triggered by physical or emotional stress, believed to be related to catecholamine surge. Animal models of TTS show neutrophil infiltration and subsequent ingress of macrophages mediating inflammatory changes. Rheumatological conditions may impact TTS and require further investigation.
Article
Virology
Muneerah Smith, Houari B. Abdesselem, Michelle Mullins, Ti-Myen Tan, Andrew J. M. Nel, Maryam A. Y. Al-Nesf, Ilham Bensmail, Nour K. Majbour, Nishant N. Vaikath, Adviti Naik, Khalid Ouararhni, Vidya Mohamed-Ali, Mohammed Al-Maadheed, Darien T. Schell, Seanantha S. Baros-Steyl, Nur D. Anuar, Nur H. Ismail, Priscilla E. Morris, Raja N. R. Mamat, Nurul S. M. Rosli, Arif Anwar, Kavithambigai Ellan, Rozainanee M. Zain, Wendy A. Burgers, Elizabeth S. Mayne, Omar M. A. El-Agnaf, Jonathan M. Blackburn
Summary: The study demonstrated significant variations in the magnitude and epitope utilization of the humoral immune response to SARS-CoV-2 viral antigens within populations following natural infection, as shown through a novel protein microarray design.
Review
Rheumatology
Dominic Seet, Nur Azizah Allameen, Sen Hee Tay, Jiacai Cho, Anselm Mak
Summary: Cognitive dysfunction in patients with systemic lupus erythematosus significantly affects their quality of life, but faces challenges in diagnosis and assessment with widely varying prevalence rates. Despite the lack of standardized tests and biomarkers, progress has been made in diagnosing CD and understanding its pathogenic mechanisms.
RHEUMATOLOGY AND THERAPY
(2021)
Correction
Rheumatology
Megan R. W. Barber, Cristina Drenkard, Titilola Falasinnu, Alberta Hoi, Anselm Mak, Nien Yee Kow, Elisabet Svenungsson, Jonna Peterson, Ann E. Clarke, Rosalind Ramsey-Goldman
NATURE REVIEWS RHEUMATOLOGY
(2021)
Review
Rheumatology
Megan R. W. Barber, Cristina Drenkard, Titilola Falasinnu, Alberta Hoi, Anselm Mak, Nien Yee Kow, Elisabet Svenungsson, Jonna Peterson, Ann E. Clarke, Rosalind Ramsey-Goldman
Summary: Systemic lupus erythematosus (SLE) is an autoimmune disease that predominantly affects young women, with certain ethnic groups experiencing higher vulnerability and mortality rates. Global incidence and prevalence of SLE vary widely due to differences in population, environment, and socioeconomic factors. Inconsistent reporting is also influenced by variations in study design and case definitions.
NATURE REVIEWS RHEUMATOLOGY
(2021)
Article
Rheumatology
Xing Qian, Fang Ji, Kwun Kei Ng, Amelia Jialin Koh, Beatrice Rui Yi Loo, Mary Charlotte Townsend, Ofer Pasternak, Sen Hee Tay, Juan Helen Zhou, Anselm Mak
Summary: Brain white matter microstructural changes and increased extracellular free water (FW) volume are observed in SLE patients. The increased extracellular FW is associated with reduced neurocognitive performance, particularly in sustained attention, and higher cumulative glucocorticoid dose.
Article
Rheumatology
Jonathan T. W. Au Eong, Aisha Lateef, Shen Liang, Sandy H. H. Lim, Sen Hee Tay, Anselm Mak, Jiacai Cho
Summary: This study compared the subsequent disease activity and flares among SLE patients who received teleconsultation or in-person consultation. The results showed that there was no significant difference in disease activity and flares between the two forms of consultations, and physicians' prescription behavior was also similar.
Letter
Rheumatology
Dominic Seet, Shir Ying Lee, Melissa Ooi, Ju Ee Seet, Jiacai Cho, Anselm Mak
ARTHRITIS & RHEUMATOLOGY
(2022)
Review
Rheumatology
Manuel Francisco Ugarte-Gil, Anselm Mak, Joanna Leong, Bhushan Dharmadhikari, Nien Yee Kow, Cristina Reategui-Sokolova, Claudia Elera-Fitzcarrald, Cinthia Aranow, Laurent Arnaud, Anca D. Askanase, Sang-Cheol Bae, Sasha Bernatsky, Ian N. Bruce, Jill Buyon, Nathalie Costedoat-Chalumeau, Mary Ann Dooley, Paul R. Fortin, Ellen M. Ginzler, Dafna D. Gladman, John Hanly, Murat Inanc, David Isenberg, Soren Jacobsen, Judith A. James, Andreas Jonsen, Kenneth Kalunian, Diane L. Kamen, Sung Sam Lim, Eric Morand, Marta Mosca, Christine Peschken, Bernardo A. Pons-Estel, Anisur Rahman, Rosalind Ramsey-Goldman, John Reynolds, Juanita Romero-Diaz, Guillermo Ruiz-Irastorza, Jorge Sanchez-Guerrero, Elisabet Svenungsson, Murray Urowitz, Evelyne Vinet, Ronald F. van Vollenhoven, Alexandre Voskuyl, Daniel J. Wallace, Michelle A. Petri, Susan Manzi, Ann Elaine Clarke, Mike Cheung, Vernon Farewell, Graciela S. Alarcon
Summary: This study confirmed associations between GC use and three specific damage items, suggesting that in treating patients with SLE, the goal should be to maximize the efficacy of GC while minimizing their harms.
LUPUS SCIENCE & MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Tao Ming Sim, Siying Jane Ong, Anselm Mak, Sen Hee Tay
Summary: This review discusses the bench to bedside translation of the type I IFN pathway and explores some unresolved issues when selecting SLE patients for treatment with biologics targeting type I IFNs.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Rheumatology
Anselm Mak, Jerry Kok Yen Chan
Summary: The excessive cardiovascular mortality observed in patients with SLE compared to the general population has led to in-depth investigations of non-traditional, SLE-related risk factors such as endothelial dysfunction and reduced EPC levels. Challenges in defining and assessing EPCs pose difficulties in translating them into prognostic markers for CVD in SLE patients.
NATURE REVIEWS RHEUMATOLOGY
(2022)
Review
Cell Biology
Anselm Mak
Summary: The complexities of T cells in SLE and the potential role of IL-2 in the pathophysiological process of SLE are discussed in this article. Low-dose IL-2 administration shows promise as a therapeutic approach for SLE.
Article
Rheumatology
Jinghui Huang, Nien Yee Kow, Hui Yin Lee, Anna-Marie Fairhurst, Anselm Mak
Summary: SLE patients have significantly lower levels of CAC compared to HC, and the use of HCQ is associated with a more discrepant CAC level between the two groups. This study calls for further research to explore the beneficial impact of HCQ on CAC functionality in SLE patients.