Article
Chemistry, Analytical
Yumei Liu, Hao Yang, Rui Wan, Mohammad Rizwan Khan, Nan Wang, Rosa Busquets, Ruijie Deng, Qiang He, Zhifeng Zhao
Summary: The ratiometric aptamer probes proposed here can robustly monitor Pb2+ in food samples by leveraging the transition of G-quadruplex structural response to Pb2+, monitored by NMM, and utilizing specific dye and terminal-labeled fluorophore for ratiometric signal outputs towards Pb2+, demonstrating feasibility for on-site Pb2+ testing associated with food safety.
Article
Chemistry, Analytical
Tongtong Li, Yan Zhang, Xiaohong Sun, Yanjin Zhang, Yongan Wang, Zhiyong Nie
Summary: A fluorescent probe was developed for ratiometric detection of thallium ions in mineral water samples by modifying a G-rich aptamer. The probe changes its structure from a random coil to a G-quadruplex upon binding with Tl+, allowing for fluorescence resonance energy transfer between the donor and acceptor moieties. Under optimized conditions, the probe provided satisfactory results with a detection limit of 30.1 μM and a linear concentration range of 10 μM-10 mM.
Article
Chemistry, Multidisciplinary
Lin Jiang, Lu Zhou, Jialin Sai, Dongguo Xue, Lijun Xu, Renjun Pei
Summary: The detection of carbendazim (CZ) fungicide residues in food has attracted increasing attention. However, most existing methods are complex, costly, and have low throughput. In this study, a label-free fluorescent aptasensor was developed for CZ detection, based on a G-quadruplex-specific probe. The formed G-quadruplex structure activated the fluorescence of the fluorescent probe N-methyl mesoporphyrin IX (NMM) in the presence of CZ. A linear relationship between fluorescence and CZ concentration was achieved within the range of 0.05-0.4 μM, with a detection limit of 24.28 nM. This study proposes a new approach for the design of label-free aptasensors.
Article
Chemistry, Analytical
Mingming Xu, Yu Peng, Huan Liu, Xinyu Tian, Hualin Yang, Yu Zhou
Summary: A simple ratiometric method was developed to detect Hg2+ based on the structural change between G-quadruplex structure and dsDNA. The method showed a linear response within the range of 0.7-2.5 µM Hg2+ with a detection limit of 9.3 nM, demonstrating good selectivity and potential application for Hg2+ detection in real environmental samples.
Article
Materials Science, Multidisciplinary
Rana Bagheri, Fathallah Karimzadeh, Ahmad Kermanpur, Mahshid Kharaziha
Summary: A simple and novel method for immobilization of AS1411 aptamer on Cu nanoparticles electrode was presented and validated by experiments. The study found that choosing 0.03 M Cu concentration, 10 μM aptamer concentration, and 20 minutes incubation time achieved the optimized performance, which was confirmed by quartz crystal microbalance.
Article
Biotechnology & Applied Microbiology
Peng Li, Wenqin Li, Zhuohao Xie, Haonan Zhan, Liehua Deng, Jiahao Huang
Summary: This study developed an innovative colorimetric assay method for the determination of carcinoembryonic antigen using aptamer probes and DNA hybridization chain reaction for signal amplification. The method showed good selectivity and sensitivity and can be easily extended for the detection of other analytes.
BIOTECHNOLOGY AND BIOENGINEERING
(2022)
Article
Chemistry, Analytical
Jingjing Jiang, Xuezhong Du
Summary: The label-free ratiometric electrochemiluminescence (ECL) sensing strategy proposed in this study utilizes G-quadruplex/hemin-regulated ECL emissions of CdS quantum dots and luminol as the coreactant for the sensitive detection of target DNA. The ECL biosensor shows a wide response range for T-DNA detection, low detection limit, and excellent selectivity against mismatched base sequences, providing a reliable and sensitive sensing platform for the detection of targets in the analytical community.
Article
Spectroscopy
Dilan Guner, Berke Bilgenur Sener, Ceren Bayrac
Summary: Auramine o (AO) is a synthetic dye used in paper and textile industries. Although it has been banned as a food additive in many countries, it is still illegally used in certain food products and pharmaceuticals. Monitoring the presence of AO in food products is important to minimize health risks. This study developed a simple, sensitive, and selective label-free detection method based on G-quadruplex for AO. The method showed high specificity for AO and was successfully applied to detect AO in pasta, bulgur, and curry powder.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2022)
Article
Instruments & Instrumentation
Jun Deng, Mengyu Niu, Xingquan Liu, Jin Feng, Shuang Ji, Zhijun Guo
Summary: In this experimental research, a label-free sensing strategy utilizing aptamers and fluorescence probes exhibited high selectivity and low detection limit for adenosine triphosphate. The experimental procedures were validated and the designed approach was successfully applied to monitor ATP concentrations in cell extracts, showing promising implications for bioanalytical science research.
APPLIED SPECTROSCOPY
(2021)
Article
Biochemistry & Molecular Biology
David Moreira, Daniela Leitao, Jessica Lopes-Nunes, Tiago Santos, Joana Figueiredo, Andre Miranda, Daniela Alexandre, Candida Tomaz, Jean-Louis Mergny, Carla Cruz
Summary: In this study, the formation and stability of G-quadruplex structure of a G-rich DNA aptamer AT11-L2 were explored under different experimental conditions, along with its interactions with various G4 ligands and nucleolin (NCL). The results showed that AT11-L2 adopted different topologies depending on the KCl concentration, and the addition of ligands significantly stabilized the G-quadruplex structure. Furthermore, a specific interaction between AT11-L2 G-quadruplex and NCL was identified, and co-localization of these two molecules was observed in certain cell lines.
Article
Biochemistry & Molecular Biology
Chengcong Zhu, Lisha Gong, Yang Yang
Summary: In this study, we developed an exponential amplification-based label-free method for detecting exosomes, which can detect exosomes at low concentrations and has potential clinical applications.
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
(2022)
Review
Chemistry, Physical
Jerome Dejeu, Angeline Van der Heyden, Nicolas Spinelli, Eric Defrancq, Liliane Coche-Guerente
Summary: Aptamer-based electrochemical sensors have been developed for detecting a wide variety of analytes with high selectivity, sensitivity, and stability, using specific G-quadruplex (G4) conformations for molecular recognition. These sensors can be miniaturized, have low production costs, and offer great flexibility in design assemblies.
CURRENT OPINION IN ELECTROCHEMISTRY
(2021)
Article
Veterinary Sciences
Ying Wang, Rong Li, Yang Zhang, Weida Zhang, Sishun Hu, Zili Li
Summary: A visual detection method based on CRISPR-Cas12a has been developed for highly sensitive detection of ASFV and PCV2. This method is simple, convenient, and does not require instruments or power, making it suitable for field use.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Review
Biochemistry & Molecular Biology
Weronika Kotkowiak, Anna Pasternak
Summary: G-quadruplexes play a crucial role in nucleic acid structures and can be utilized as regulatory elements in cells. Extending quadruplexes with additional structural elements enhances stability and biological activity, and the specific interaction of G-quadruplexes with target molecules is pivotal. Future applications of G-quadruplex junctions hold promise, and there is still room for development in emerging research areas.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Chemistry, Analytical
Xuhua Zhao, Yanan Zhou, Xuzhen Lv, Hanxiao Zhang, Xiaohua Cui, Tao Gong, Hongmin Meng, Litian Yin, Gaopeng Li, Baofeng Yu
Summary: By incorporating a split G-quadruplex structure into a stem-loop structure, a CRISPR/Cas12a-based biosensor with faster cleavage speed and unaffected trans-cleavage rate on G-quadruplex was constructed. This biosensor is label-free and highly selective, offering great potential in medical diagnoses and biology science.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Biochemistry & Molecular Biology
Jie Feng, Fengming He, Yuhui Huang, Mi Zhou, Xiangzhong Liu, Xiansheng Ye, Renjing Yang, Wenjing Tian, Haifeng Chen
Summary: Two undescribed phenolic glycosides were isolated from the Trollius chinensis Bunge plant, showing different inhibitory effects on alpha-glucosidase. Spectroscopic analysis revealed the structural changes of the enzyme during binding with these compounds.
Article
Engineering, Ocean
Shujin Zhou, Mi Zhou, Yinghui Tian, Xihong Zhang
Summary: In this study, a numerical model is established to investigate the soil flow mechanism of caissons with pad-eye stiffeners. The soil strain softening effect is considered. The study finds that the soil failure mechanisms and penetration resistances of locally stiffened caissons at the pad-eye are significantly different from unstiffened caissons or caissons with interval rings. A simplified flow mechanism is proposed and approximating expressions are derived for predicting the penetration resistance of the caisson.
APPLIED OCEAN RESEARCH
(2022)
Article
Engineering, Civil
Kewei Liu, Megan Walske, Mi Zhou, Xihong Zhang
Summary: The use of rammed earth as a construction material has gained attention for its sustainability and low-cost potential. However, the mechanical performance of stabilized rammed earth, particularly under dynamic loading, is not well understood. This study experimentally investigates the quasi-static and dynamic material properties of cement and calcium carbonate residue stabilized rammed earth, and derives empirical formulae for the dynamic increase factor.
INTERNATIONAL JOURNAL OF PROTECTIVE STRUCTURES
(2023)
Article
Physics, Fluids & Plasmas
Quanxin Li, Jianguo Wang, Zixuan Gong, Li Cai, Mi Zhou, Jing Xiao
Summary: This article presents an analysis of electric fields and derivative waveforms radiated from lightning return stroke with an attachment point above the ground. The presence of the attachment point causes a noticeable initial sharp peak in the electric fields, with larger positive peaks and negative overshoot in the derivative waveform.
IEEE TRANSACTIONS ON PLASMA SCIENCE
(2022)
Article
Engineering, Electrical & Electronic
Jianguo Wang, Zhiling Xu, Li Cai, Yuquan Fang, Dai Lu, Huan Huang, Mi Zhou, Yadong Fan
Summary: This paper presents the measurement of three-phase impulse overvoltage at the terminal of a 10 kV distribution line when impulse current is injected into the phase conductor. The study reveals that the struck phase overvoltage consists of three components, including negative pulse, voltage maintenance stage, and overshoot oscillation. The negative peak values of the struck phase overvoltage were observed to range from -85.4 kV to -96.0 kV, depending on the injected impulse levels. There is a strong positive relationship between the oscillation amplitude of the struck phase overvoltage and the injected current amplitude.
IEEE TRANSACTIONS ON POWER DELIVERY
(2022)
Article
Polymer Science
Zejian He, Haifeng Shao, Nannan Zhang, Jie Li, Hai Xiao, Tianhang Weng, Mi Zhou, Bianying Wen, Yulong Chen
Summary: This study designed and synthesized three amide nucleating agents with different symmetries to investigate the effect of symmetry on PLLA crystallization. It was found that the C3 symmetric amide structure exhibited the best performance as an efficient nucleation site.
JOURNAL OF POLYMER SCIENCE
(2023)
Review
Engineering, Environmental
Huijuan Wu, Weiqiong Zheng, Ran Zhu, Mi Zhou, Xiancheng Ren, Yinghan Wang, Chong Cheng, Hongju Zhou, Sujiao Cao
Summary: This article comprehensively summarizes and discusses the most important advances in engineering MOF nanoarchitectures for efficient water electrolysis. First, the methods of modulating the coordination structures and metal atom environments of MOF and MOF-derived catalysts are reviewed, and then their applications in water splitting are discussed based on the coordination structure and local atomic environment.
CHEMICAL ENGINEERING JOURNAL
(2023)
Article
Environmental Sciences
Bing Yang, Mi Zhou, Yuan Meng, Keming Chen, Jie Xu, Xiangfu Huang, Yucheng Liu, Lingli Li, Lili Ma, Mingyan Chen
Summary: Chemical oxidation as a pretreatment for petroleum-contaminated soil, coupled with bioremediation, can have serious impacts on indigenous microorganisms and available nutrients. This study found that hydrogen peroxide treatments significantly promoted the removal of total petroleum hydrocarbons (TPH), especially in the initial days, and also affected the levels of dissolved nutrients. Both chemical oxidation and nutrients contribute to changes in microbial community structure and diversity. However, the long-term effects of H2O2 as a pretreatment had little impact on the soil indigenous microbial community structure and actually facilitated the continuous removal of TPH by indigenous microorganisms.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2023)
Article
Engineering, Electrical & Electronic
Runyu Fu, Jianguo Wang, Jianping Wang, Mi Zhou, Cai Li, Yating Zhao, Yadong Fan
Summary: This paper establishes a holistic model of a 500 kV GIS step-up substation equipment in a coastal power plant using the Electromagnetic Transient Program (EMTP). It investigates the VFTO waveform generated in different operations and analyzes its impact on equipment.
IET GENERATION TRANSMISSION & DISTRIBUTION
(2023)
Article
Engineering, Electrical & Electronic
Mi Zhou, Weihan Zhao, Shengquan Zheng, Yongming Guo, Zehong Yang, Jianguo Wang, Li Cai, Yadong Fan
Summary: This study establishes a simulation model to investigate the lightning inductive characteristics between parallel cables. The research findings indicate that an increase in the horizontal distance between cables leads to a decrease in the cable induced voltage. Additionally, linear correlations exist between the height difference or disturbed cable length and the cable induced voltage, reaching maximum when the cables are at the same height and of equal length.
IET SCIENCE MEASUREMENT & TECHNOLOGY
(2023)
Article
Spectroscopy
DongFei Li, Shuo Zhang, Hongsheng Jia, Mingxing Song, Ying Zhang, Haibo Li, Mi Zhou
Summary: In this study, high pressure Raman research was conducted on lithium perchlorate crystal, and it was found that the crystal undergoes phase transformations under different pressure conditions, which has significant implications for its performance.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2023)
Article
Engineering, Environmental
Mei Zhang, Yuting Wen, Zhangheng Huang, Xin Qin, Mi Zhou, Dexuan Xiao, Weitong Cui, Zhiqiang Liu, Yunfeng Lin
Summary: A novel nanomedicine was developed for the treatment of rheumatoid arthritis, which carries methotrexate and uses aptamer targeting tumor necrosis factor-alpha. This targeted nanomedicine accumulates in the inflammatory joints, reducing systemic toxicity and decreasing joint damage.
CHEMICAL ENGINEERING JOURNAL
(2023)
Review
Chemistry, Multidisciplinary
Tiantian Li, Yuting Deng, Xiao Rong, Chao He, Mi Zhou, Yuanjiao Tang, Hongju Zhou, Chong Cheng, Changsheng Zhao
Summary: This review comprehensively summarizes the main advances and applications of tellurium-based nanomaterials (Te NMs) in electrocatalysis. The engineering strategies and principles to enhance the electrocatalytic activity and stability of Te NMs are discussed, including chemical/physical/multiplex templating strategies, heteroatom doping, and phase engineering. The typical applications of Te NMs in electrocatalysis are also presented. Finally, the key issues, challenges, and development trends of Te NMs as electrocatalysts are highlighted.
Article
Chemistry, Multidisciplinary
Yu-long Chen, Yue-song Mu, Ze-jian He, Xin-ming Pu, Dong-qi Wang, Mi Zhou, Li-ping Yang
Summary: This study focuses on the synthesis and characterization of poly(ethylene 2,5-tetrahydrofuran dimethyl terephthalate; PEFTs), a bio-based material with potential applications in hydrophobic nanofiber and filter films.
Article
Green & Sustainable Science & Technology
Yixin Guo, Pan He, Tim D. Searchinger, Youfan Chen, Marco Springmann, Mi Zhou, Xin Zhang, Lin Zhang, Denise L. Mauzerall
Summary: This study examines the implications of four potential dietary shifts in China on air pollution, GHG emissions, carbon storage loss, water use, and human health. The results show that replacing red meat with soy benefits the environment, while adopting the Chinese Dietary Guideline and EAT-Lancet diets can prevent premature deaths. However, these dietary shifts also increase water use and GHG emissions.
Article
Chemistry, Applied
M. Graca Dias, Elsa Vasco, Francisco Ravasco, Lufsa Oliveira
Summary: This study estimated the vitamin D intake of "adults" and "elderly" populations in Portugal using the TDS methodology. The results showed that the majority of people had inadequate vitamin D intake, well below the Dietary Reference Values.
Article
Chemistry, Applied
Yanan Wang, Jiachen Shi, Yong-Jiang Xu, Chin-Ping Tan, Yuanfa Liu
Summary: This study investigates the variations in lipid digestion profiles among individuals of different ages using in vitro digestion models. The findings suggest that adults have a more comprehensive lipid digestion compared to infants, and infants tend to release shorter chain length and more saturated free fatty acids during digestion. Additionally, the particle sizes in the stomach of the elderly were consistently larger. This study enhances our understanding of how lipids with different degrees of unsaturation undergo digestion in diverse age groups.
Article
Chemistry, Applied
Hyunjong Yu, Huisu Kim, Pahn-Shick Chang
Summary: Chitosan-pectin emulsion-filled hydrogel (EFH) was developed to enhance the bioaccessibility of lipophilic bioactive compounds through intestinal delivery. The EFH, prepared without crosslinking agents, demonstrated improved mechanical strength and compactness with higher pectin concentration. It retained the emulsion at pH 2.0 and released it at pH 7.4, resulting in enhanced release of free fatty acids and improved bioaccessibility of curcumin.
Article
Chemistry, Applied
Tongze Zhang, Siqi Hong, Jia-Rong Zhang, Pin-He Liu, Siyi Li, Zixian Wen, Jianwei Xiao, Guirong Zhang, Olivier Habimana, Nagendra P. Shah, Zhongquan Sui, Harold Corke
Summary: Lactic acid fermentation significantly affects the morphology and physicochemical properties of proso millet starch, including the formation of surface indentations and small pores, decrease in gelatinization temperatures, and changes in hardness and adhesiveness.
Article
Chemistry, Applied
Liqin Kong, Feng Hong, Peng Luan, Yiping Chen, Yaoze Feng, Ming Zhu
Summary: This study presents a novel impedance biosensor using composite nanomaterials and T1R1 as a signal probe, which can competitively and ultra-sensitively detect umami intensity. The biosensor exhibits exceptional analytical performance and is suitable for food flavor evaluation.
Article
Chemistry, Applied
Kunying Lv, Yixin Yang, Qilong Li, Ran Chen, Liang Deng, Yiwei Zhang, Ning Jiang
Summary: Horse's milk, with its high nutritional value and low allergenic proteins, could be a substitute for cow's milk in infant consumption. A proteomic method was used to identify and compare milk fat globule membrane (MFGM) and whey proteins from different horse breeds. The study found differences in protein composition and functionality, which could support the development of formulas more suitable for human infants.
Article
Chemistry, Applied
Enrique Jacobo Diaz-Montana, Helene Brignot, Ramon Aparicio-Ruiz, Thierry Thomas- Danguin, Maria Teresa Morales
Summary: Sensory perception of virgin olive oil is influenced by phenols and volatiles, which are affected by the composition of the oil and biological factors. This study investigated the effect of saliva and phenols on the release of volatiles, and found that the presence of phenols decreased the release of saturated volatiles.
Article
Chemistry, Applied
Wei Zhou, Rui Zhang, Zhen Cai, Fangfang Wu, Yong Hu, Chao Huang, Kun Hu, Yun Chen
Summary: Environmentally friendly and outstanding pH-responsive cationic starch nanoparticles (CSNP) were prepared from pH-sensitive starch. CSNP exhibited nanosize and regular sphere, highly free-flowing molecular chains, and demonstrated excellent pH responsiveness through multiple emulsion/demulsification transitions.
Article
Chemistry, Applied
Andrea Koo, Vinayak Ghate, Weibiao Zhou
Summary: This study suggests that direct seeding may negatively affect the nutritional quality of crops, causing a decrease in ascorbic acid, vitamin K, and total glucosinolate content.
Article
Chemistry, Applied
Tonghao Du, Yazhou Xu, Xiaoyan Xu, Shijin Xiong, Linli Zhang, Biao Dong, Jinqing Huang, Tao Huang, Muyan Xiao, Tao Xiong, Mingyong Xie
Summary: This study successfully improved the ACE inhibitory activity of black sesame seeds by fermenting them with Lactobacillus Plantarum NCU116 and hydrolyzing them using acid protease. The RF-PSO model was used to predict the ACE inhibitory activity during the hydrolysis process. Eight peptides with ACE inhibitory activity were identified from fermented black sesame seed hydrolysates after separation and screening.
Article
Chemistry, Applied
Yao Liu, Songyi Lin, Kexin Liu, Shan Wang, Qiaozhen Liu, Na Sun
Summary: This study analyzed the structural changes of shrimp proteins during digestion, predicted the immunodominant epitopes, and validated their allergenicity. The results showed that shrimp proteins were degraded into peptides during digestion, but still carried IgE epitopes that trigger allergic reactions.
Article
Chemistry, Applied
Tiantian Fu, Hongwei Cao, Yu Zhang, Xiao Guan
Summary: This study investigates the impact of milling on the active components in rice, with a focus on the stability and bioaccessibility of phenols, VB1, and alpha-GABA during cooking and digestion. The findings show that milling exacerbates the instability of gamma-GABA during cooking and VB1 during digestion, and it affects the bioaccessibility of these active compounds.
Article
Chemistry, Applied
Zhihao Yang, Yanru Hou, Min Zhang, Puxin Hou, Chang Liu, Lu Dou, Xiaoyu Chen, Lihua Zhao, Lin Su, Ye Jin
Summary: This study investigated the molecular mechanism of feeding regimes on lamb flavor by using TMT labeling combined with MS. The results showed that pasture-fed groups had higher levels of amino acids and volatile flavor substances compared to concentrate-fed groups. Additionally, several differentially abundant proteins associated with lamb flavor were identified.
Article
Chemistry, Applied
Zixuan Xie, De Zhang, Junyu Zhu, Qianqian Luo, Jun Liu, Jingtao Zhou, Xiaoyong Wang, Yuqiong Chen, Zhi Yu, Dejiang Ni
Summary: This study investigated the acidification of aroma-enhanced black tea during storage. Analysis of non-volatile substances and organic acids using UPLC-Q-TOF/MS and HPLC revealed a decrease in soluble sugars and amino acids, while an increase in organic acids such as oxalic acid, malic acid, and quinic acid. In vitro experiments further demonstrated that the acidification is a result of the decomposition of sugars and amino acids by heating, as well as the oxidation of aromatic aldehydes. Additionally, the study showed that the taste composition of tea infusion is altered, with reduced amino acids, catechins, soluble sugars, and flavonoids. This research provides a theoretical basis for improving the quality of black tea.
Article
Chemistry, Applied
Lin Wang, Falai Ma, Zihan Li, Yan Zhang
Summary: This study developed time-temperature integrators based on amyloglucosidase@Cu3(PO4)2 nanoflowers for monitoring the freshness of chilled pork. The results showed that the integrators were highly reliable and accurate in predicting the quality of chilled pork.