Article
Environmental Sciences
Arthur David, Jade Chaker, Elliott J. Price, Vincent Bessonneau, Andrew J. Chetwynd, Chiara M. Vitale, Jana Klanova, Douglas I. Walker, Jean-Philippe Antignac, Robert Barouki, Gary W. Miller
Summary: The use of high-resolution mass spectrometry to characterize the human internal chemical exposome provides unprecedented precision in investigating the environmental etiology of chronic diseases. However, improvements are needed in the flexibility, sensitivity, and automation of analytical techniques. Additionally, challenges exist in assessing the dynamic nature of the internal chemical exposome, establishing international collaboration, high level networking, and sustainable research infrastructure.
ENVIRONMENT INTERNATIONAL
(2021)
Article
Genetics & Heredity
In-Hee Lee, Matthew Ryan Smith, Azam Yazdani, Sumiti Sandhu, Douglas I. I. Walker, Kenneth D. D. Mandl, Dean P. P. Jones, Sek Won Kong
Summary: This study investigates the genetic and environmental factors influencing metabolite levels in children, utilizing high-resolution mass spectrometry combined with genome-wide genotyping. The results reveal a wide range of genetic influence on diverse chemical species and metabolic pathways.
Article
Biochemistry & Molecular Biology
Antonia Fecke, Nay Min Min Thaw Saw, Dipali Kale, Siva Swapna Kasarla, Albert Sickmann, Prasad Phapale
Summary: Quantifying metabolites from various biological samples is necessary for the clinical and biomedical translation of metabolomics research. One of the ongoing challenges in biomedical metabolomics studies is the large-scale quantification of targeted metabolites, mainly due to the complexity of biological sample matrices. In this study, we developed and validated a metabolomics method for over 280 standard metabolites and quantified over 90 metabolites using the relative response factor (RRF) approach combined with an integrated analytical and computational workflow.
Review
Chemistry, Analytical
Tuulia Hyotylainen
Summary: Chemical exposures are likely important in human chronic diseases, but the specific mechanisms are poorly understood. Due to the complex and challenging nature of chemical exposome research, there is limited understanding of its impact on disease development.
JOURNAL OF SEPARATION SCIENCE
(2021)
Editorial Material
Microbiology
Joris J. R. Louwen, Justin J. J. van der Hooft
Summary: Microbial specialized metabolites play a crucial role in host-microbiome interactions, and there is a need for new methods to utilize the expanding genomic and metabolomic datasets while connecting them to structural and functional knowledge inferred from transcriptomics and proteomics experiments. This article describes current approaches for comprehensive mining of metabolomics and genomics data, as well as proposes a vision for automated linking of omics data of specialized metabolites to their structures, biosynthesis pathways, producers, and functions.
Article
Chemistry, Analytical
Wangjie Lv, Zhongda Zeng, Yuqing Zhang, Qingqing Wang, Lichao Wang, Zhaoxuan Zhang, Xianzhe Shi, Xinjie Zhao, Guowang Xu
Summary: In this study, an alternate RPLC-MS method was developed to quantitatively determine metabolites and lipids. A wide set of reference standards and real samples were used to define multiple reaction monitoring (MRM) transitions for metabolomic and lipidomic analyses, resulting in the quantification of over 1000 metabolites and lipids. The developed method showed good analytical characteristics and was successfully validated using standard reference material.
ANALYTICA CHIMICA ACTA
(2022)
Article
Biochemical Research Methods
Konstantinos Tzanakis, Tim W. Nattkemper, Karsten Niehaus, Stefan P. Albaum
Summary: MetHoS is an automated web-based software platform for processing and analyzing large-scale metabolomics data sets using mass spectrometry. It utilizes a big data framework for parallel processing and distributed storage, enabling comprehensive analysis of hundreds or even thousands of experiments.
BMC BIOINFORMATICS
(2022)
Article
Public, Environmental & Occupational Health
Mika Ala-Korpela, Siyu Zhao, Marjo-Riitta Jarvelin, Ville-Petteri Makinen, Pauli Ohukainen
Summary: Quantitative analysis of lipoproteins using NMR spectroscopy is common in large-scale studies, with a methodology allowing for comprehensive characterization of lipoprotein subclasses. In this study, serum samples from two Finnish birth cohorts were analyzed using NMR, revealing the important relationship between lipoprotein particle concentrations and size. The findings suggest that lipoprotein particles are under strict metabolic constraints, impacting their composition and concentrations.
INTERNATIONAL JOURNAL OF EPIDEMIOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Ying Chen, Jian Guo, Shipei Xing, Huaxu Yu, Tao Huan
Summary: This study developed a metabolomics workflow to analyze short-term and long-term exposures as well as endogenous metabolites in the same hair sample. The analytical approach involved extraction of short-term exposures through hair washing and extraction of endogenous metabolites and long-term exposures through mechanical homogenization. By processing the data with bioinformatics programs, the study demonstrated a novel analytical approach for global-scale analysis of hair metabolome and exposome.
FRONTIERS IN CHEMISTRY
(2021)
Article
Chemistry, Analytical
Teresa S. McMillen, Andrew Leslie, Kenneth Chisholm, Susanne Penny, Jeffrey Gallant, Alejandro Cohen, Arik Drucker, James P. Fawcett, Devanand M. Pinto
Summary: This study presents a comprehensive and quantitatively robust method for metabolomic analysis, using HILIC and RP stationary phases combined with positive and negative electrospray ionization. The method allows for complete analysis of a single sample in as little as 40 minutes and has been validated with clinical samples.
ANALYTICA CHIMICA ACTA
(2023)
Article
Energy & Fuels
Yanan Wang, Xuebing Han, Xiaodong Xu, Yue Pan, Feng Dai, Daijiang Zou, Languang Lu, Minggao Ouyang
Summary: This paper proposes a comprehensive data-driven assessment scheme called ComDAS to improve the multi-tasks realization for cloud platform. It can be fast deployed for lithium-ion batteries of EVs, and integrates capacity estimation, anomaly detection, and variation evaluation. Neural network is applied for calibration of capacity estimation to achieve higher accuracy. A risk scoring method is proposed to achieve long-scale statistics filtering in time dimension, and weeks-early risk pre-warning for anomaly detection. Correlation analysis is also performed to conduct cross validations and reveal the coupling evolution mechanism among capacity, anomaly, and variation.
JOURNAL OF ENERGY STORAGE
(2023)
Article
Nutrition & Dietetics
Yuri Bastos-Moreira, Lionel Ouedraogo, Marthe De Boevre, Alemayehu Argaw, Brenda de Kok, Giles T. Hanley-Cook, Lishi Deng, Moctar Ouedraogo, Anderson Compaore, Kokeb Tesfamariam, Rasmane Ganaba, Lieven Huybregts, Laeticia Celine Toe, Carl Lachat, Patrick Kolsteren, Sarah De Saeger, Trenton Dailey-Chwalibog
Summary: This study aims to evaluate the physiological effect of multi-micronutrient-fortified BEP supplementation on pregnant and lactating women and their infants. Biological specimens will be collected and multi-omics profiles and biomonitoring markers will be used to assess the effects of BEP supplementation on maternal health, birth outcomes, and infant growth.
Article
Cardiac & Cardiovascular Systems
Chengcan Lu, Chunyan Liu, Di Mei, Mengjie Yu, Jian Bai, Xue Bao, Min Wang, Kejia Fu, Xin Yi, Weihong Ge, Jizhong Shen, Yuzhu Peng, Wei Xu
Summary: Using human humoral metabolomic profiling, this study aimed to discover diagnostic biomarkers and pathogenesis of atrial fibrillation (AF). By analyzing metabolic pathways, the study identified altered metabolites as potential biomarkers for the onset, progression, and differential diagnosis of AF.
FRONTIERS IN CARDIOVASCULAR MEDICINE
(2022)
Article
Oncology
Eliza M. Gordon-Lipkin, Christopher S. Marcum, Shannon Kruk, Elizabeth Thompson, Sophie E. M. Kelly, Heather Kalish, Lorenza Bellusci, Surender Khurana, Kaitlyn Sadtler, Peter J. McGuire
Summary: This study found that viral infections are pervasive in children with mitochondrial disease despite families adhering to risk mitigation behaviors during the COVID-19 pandemic. Not all infections resulted in illness in the children, suggesting some were subclinical or asymptomatic. However, selected children with mitochondrial disease did experience neurological events. The study emphasizes the need for further understanding of host-pathogen interactions through broad serologic surveillance.
CLINICAL AND TRANSLATIONAL MEDICINE
(2022)
Article
Food Science & Technology
Su-Hyun Kim, Digar Singh, Su Young Son, Sunmin Lee, Dong Ho Suh, Na-Rae Lee, Gun-Seok Park, Jihee Kang, Choong Hwan Lee
Summary: This study investigated the composition and changes of intracellular and extracellular metabolome of Lactiplantibacillus plantarum under different growth times using multi-platform metabolomics. A total of 168 metabolites were identified through both non-targeted profiling and targeted quantification, among which 41 and 110 metabolites in the intra- and extracellular metabolomes showed significant differences. An expanded metabolic pathway map was constructed, highlighting the increase of bioactive compounds produced by amino acid and fatty acid metabolism in the extracellular metabolome during growth. The study demonstrated the usefulness of a multi-platform approach in identifying metabolites and provided insights into the metabolic processes involved in the growth of L. plantarum HAC01.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2023)
Article
Nutrition & Dietetics
Pol Castellano-Escuder, Raul Gonzalez-Dominguez, Marie-France Vaillant, Patricia Casas-Agustench, Nicole Hidalgo-Liberona, Nuria Estanyol-Torres, Thomas Wilson, Manfred Beckmann, Amanda J. Lloyd, Marion Oberli, Christophe Moinard, Christophe Pison, Jean-Christian Borel, Marie Joyeux-Faure, Mariette Sicard, Svetlana Artemova, Hugo Terrisse, Paul Dancer, John Draper, Alex Sanchez-Pla, Cristina Andres-Lacueva
Summary: This study aims to identify urinary metabolites as reliable biomarkers for a healthy diet index. The results showed several polyphenol microbial metabolites were positively associated with the diet index and some metabolites were associated with the intake of coffee, red meat, and fish. This suggests that urinary metabolites, particularly microbiota-derived metabolites, can be reliable indicators of adherence to healthy dietary habits.
FRONTIERS IN NUTRITION
(2022)
Article
Food Science & Technology
Raul Gonzalez-Dominguez, Ana Sayago, Maria Santos-Martin, Angeles Fernandez-Recamales
Summary: In this study, a hollow-fiber liquid-phase micro-extraction method was optimized for the simultaneous derivatization, extraction, and pre-concentration of butyltin species in commercial fruit juices. The application of this technique to commercial samples showed that the levels of organotin species in packed fruit juices are negligible.
FOOD ANALYTICAL METHODS
(2023)
Article
Food Science & Technology
Tomas Merono, Gregorio Peron, Giorgio Gargari, Raul Gonzalez-Dominguez, Antonio Minarro, Esteban Vegas-Lozano, Nicole Hidalgo-Liberona, Cristian Del Bo, Stefano Bernardi, Paul Antony Kroon, Barbara Carrieri, Antonio Cherubini, Patrizia Riso, Simone Guglielmetti, Cristina Andres-Lacueva
Summary: A polyphenol-rich diet can reduce intestinal permeability in older adults. Participants categorized according to urolithin metabotypes (UMs) exhibited different responses in the improvement of intestinal permeability after the diet. UMs identified as metabotype B showed higher improvement in intestinal permeability and exhibited changes in fatty acid metabolism, the kynurenine pathway of tryptophan catabolism, and microbial metabolization of phenolic acids. These findings are important for tailoring personalized nutrition interventions.
FOOD RESEARCH INTERNATIONAL
(2022)
Article
Biochemistry & Molecular Biology
Nuria Estanyol-Torres, Cristina Domenech-Coca, Raul Gonzalez-Dominguez, Antonio Minarro, Ferran Reverter, Jose Antonio Moreno-Munoz, Jesus Jimenez, Manel Martin-Palomas, Pol Castellano-Escuder, Hamza Mostafa, Santi Garcia-Vallve, Nerea Abasolo, Miguel A. Rodriguez, Helena Torrell, Josep M. del Bas, Alex Sanchez-Pla, Antoni Caimari, Anna Mas-Capdevila, Cristina Andres-Lacueva, Anna Crescenti
Summary: The study aimed to evaluate the effects of a mixture of four dietary fibers on obese rats. The results showed that the fiber mixture significantly reduced body weight, adiposity, HbA1c, and HDL-cholesterol levels in the rats. It also improved energy expenditure, fat oxidation, and intestinal health, while reducing adiponectin and LPS levels. The intervention effect was observed at the metabolomic, metagenomic, and transcriptomic levels.
JOURNAL OF NUTRITIONAL BIOCHEMISTRY
(2023)
Article
Medicine, Research & Experimental
Hamza Mostafa, Inken Behrendt, Tomas Merono, Raul Gonzalez-Dominguez, Mathias Fasshauer, Silvia Rudloff, Cristina Andres-Lacueva, Sabine Kuntz
Summary: This study aimed to investigate whether plasma-isolated anthocyanins and their metabolites (PAMs) modulate pancreatic cancer cell migration and to identify molecular targets of PAMs in this process. A 28-day intervention trial involving 35 healthy subjects was conducted to compare the effects of daily anthocyanin-rich juice intake versus placebo. Plasma extracts were used for migration and mechanistic in vitro studies as well as metabolomic analysis. The intake of anthocyanin-rich juice significantly reduced PANC-1 migration, and also decreased the expression of adhesion molecules in PANC-1 and endothelial cells.
BIOMEDICINE & PHARMACOTHERAPY
(2023)
Article
Nutrition & Dietetics
Jeanne Neuffer, Raul Gonzalez-Dominguez, Sophie Lefevre-Arbogast, Dorrain Y. Low, Benedicte Driollet, Catherine Helmer, Andrea Du Preez, Chiara de Lucia, Silvie R. Ruigrok, Barbara Altendorfer, Ludwig Aigner, Paul J. Lucassen, Aniko Korosi, Sandrine Thuret, Claudine Manach, Merce Pallas, Mireia Urpi-Sarda, Alex Sanchez-Pla, Cristina Andres-Lacueva, Cecilia Samieri
Summary: The gut microbiome plays a role in influencing cognition via the gut-brain axis. Higher serum levels of propionic acid, a short-chain fatty acid, were found to be associated with increased odds of cognitive decline. This effect may be mediated by hypercholesterolemia and diabetes.
Article
Public, Environmental & Occupational Health
Alvaro Gonzalez-Dominguez, Jesus Dominguez-Riscart, Maria Millan-Martinez, Alfonso Maria Lechuga-Sancho, Raul Gonzalez-Dominguez
Summary: This study examined the association between circulating blood trace elements, Mediterranean diet adherence, and childhood obesity. The findings showed that participants with higher adherence to the Mediterranean diet had better glycemic/insulinemic control, a healthier lipid profile, and increased levels of certain trace elements. These mineral alterations were closely correlated with the metabolic complications associated with childhood obesity, highlighting the importance of dietary trace elements in the pathogenesis of obesity and related disorders.
FRONTIERS IN PUBLIC HEALTH
(2023)
Article
Biochemistry & Molecular Biology
Alvaro Gonzalez-Dominguez, Jesus Dominguez-Riscart, Maria Millan-Martinez, Rosa Maria Mateos-Bernal, Alfonso Maria Lechuga-Sancho, Raul Gonzalez-Dominguez
Summary: Although puberty influences obesity progression, the molecular mechanisms underlying the role of sexual maturation in obesity-related complications are largely unknown. This study investigates the impact of puberty on oxidative stress, inflammation, and trace element blood levels in children with obesity. The findings suggest that pubertal development improves inflammatory and oxidative control in obese children, possibly through trace element-mediated buffering mechanisms.
Article
Biochemistry & Molecular Biology
Alvaro Gonzalez-Dominguez, Jesus Dominguez-Riscart, Maria Millan-Martinez, Alfonso Maria Lechuga-Sancho, Raul Gonzalez-Dominguez
Summary: Although the role of perturbed metal homeostasis in childhood obesity is well established, the association has rarely been investigated in terms of sexual dimorphism. This study examined children with obesity, with and without insulin resistance, and healthy control children, using multi-elemental analysis and measured various variables related to metabolism and hormones. The results showed that children with obesity, regardless of sex and insulin resistance status, had increased plasma copper-to-zinc ratios. However, obese girls showed increased contents of iron, zinc, and copper, suggesting a sex-dependent dysregulation in response to inflammatory processes and hyperinsulinemia.
Article
Nutrition & Dietetics
Alvaro Gonzalez-Dominguez, Maria Millan-Martinez, Jesus Dominguez-Riscart, Alfonso Maria Lechuga-Sancho, Raul Gonzalez-Dominguez
Summary: Trace elements and heavy metals play pivotal roles in childhood obesity and insulin resistance. This study found that insulin resistance may have distinct phenotypic subtypes. By analyzing plasma samples, it was discovered that delayed glucose-induced hyperinsulinemia was associated with worsened insulin resistance and lipid profile, as well as disturbed levels of chromium, cobalt, lead, and arsenic. These findings highlight the crucial involvement of altered metal homeostasis in regulating insulin signaling, glucose metabolism, and dyslipidemia in childhood obesity.
Review
Cell Biology
Simone Lista, Raul Gonzalez-Dominguez, Susana Lopez-Ortiz, Alvaro Gonzalez-Dominguez, Hector Menendez, Juan Martin-Hernandez, Alejandro Lucia, Enzo Emanuele, Diego Centonze, Bruno P. Imbimbo, Viviana Triaca, Luana Lionetto, Maurizio Simmaco, Miroslava Cuperlovic-Culf, Jericha Mill, Lingjun Li, Mark Mapstone, Alejandro Santos-Lozano, Robert Nistico
Summary: Alzheimer's disease (AD) is determined by various pathophysiological mechanisms starting 10-25 years before the onset of clinical symptoms. Metabolomics, a rapidly developing discipline, can detect and quantify hundreds/thousands of perturbed metabolites, reproducing the fluctuations of multiple networks affected by a disease. Metabolomics can help identify potential biomarkers and elucidate the pathophysiological mechanisms of AD.
AGEING RESEARCH REVIEWS
(2023)
Article
Agricultural Engineering
Marfa Santos-Martin, Juan Cubero-Cardoso, Raul Gonzalez-Dominguez, Esperanza Cortes-Trivino, Ana Sayago, Juan Urbano, Angeles Fernandez-Recamales
Summary: The development of novel green solvents is essential for the food industry to extract bioactive compounds from agrifood industry residues. In this study, an environmentally friendly ultrasound-assisted method was developed using natural deep eutectic solvents (NADES) to extract phenolic compounds from blueberry leaves. The optimal extraction efficiency was achieved using NADES composed of lactate, sodium acetate, and water (3:1:2), as well as choline chloride and oxalic acid (1:1). Compared to conventional organic solvents, these NADES showed significantly higher efficacy in the recovery of phenolic compounds and antioxidant activity. Chromatographic characterization revealed that lactic-based NADES could extract a wide range of hydroxycinnamic acids and flavonol derivatives, while choline-based NADES showed selectivity towards the extraction of anthocyanins. This method offers a promising green alternative for the recovery of phenolic compounds from plant materials and agrifood wastes, with improved extraction efficacy and selectivity compared to traditional organic solvents.
BIOMASS & BIOENERGY
(2023)
Article
Food Science & Technology
Alba Tor-Roca, Alex Sanchez-Pla, Aniko Korosi, Merce Pallas, Paul J. Lucassen, Pol Castellano-Escuder, Ludwig Aigner, Raul Gonzalez-Dominguez, Claudine Manach, Francisco Carmona, Esteban Vegas, Catherine Helmer, Catherine Feart, Sophie Lefevre-Arbogast, Jeanne Neuffer, Hyunah Lee, Sandrine Thuret, Cristina Andres-Lacueva, Cecilia Samieri, Mireia Urpi-Sarda
Summary: This study found that greater adherence to the Mediterranean diet is associated with lower odds of cognitive decline in older adults.
MOLECULAR NUTRITION & FOOD RESEARCH
(2023)
Article
Endocrinology & Metabolism
Alvaro Gonzalez-Dominguez, Thalia Belmonte, Raul Gonzalez-Dominguez
Summary: The incidence of childhood obesity and metabolic syndrome has become a major public health concern in developed countries. Oxidative stress, along with inflammation, insulin disturbances, and dyslipidemia, plays a crucial role in the development of obesity-related complications. Understanding the molecular basis of oxidative dysregulations and the involvement of miRNAs can lead to new approaches for the diagnosis, prevention, and treatment of childhood obesity and associated disorders. This literature review focuses on the association between childhood obesity, metabolic syndrome, and miRNAs, with particular emphasis on the role of miRNAs in modulating obesity-related pathogenic events, especially oxidative stress.
REVIEWS IN ENDOCRINE & METABOLIC DISORDERS
(2023)
Article
Biochemistry & Molecular Biology
Alvaro Gonzalez-Dominguez, Raul Gonzalez-Dominguez
Summary: This review article discusses the challenges of identifying reliable biomarkers in metabolomics and examines the role of inter-individual variability in this process. Additionally, future perspectives for the applicability of metabolomics in biomedical research and clinical practice are provided.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2024)