Article
Chemistry, Multidisciplinary
Preeti Sharma, Anjali Vijaykumar, Jayashree Vijaya Raghavan, Supriya Rajendra Rananaware, Alakesh Alakesh, Janhavi Bodele, Junaid Ur Rehman, Shivani Shukla, Virta S. Wagde, Savitha Nadig, Sveta Chakrabarti, Sandhya S. Visweswariah, Dipankar Nandi, Balasubramanian Gopal, Siddharth Jhunjhunwala
Summary: This study demonstrates that the uptake of non-stimulatory cargo-free particles enhances the phagocytic ability of immune cells. The increased phagocytosis is driven by changes in cell membrane fluidity and cellular compliance, and it leads to faster neutralization of pathogens and clearance in mice.
JOURNAL OF CONTROLLED RELEASE
(2022)
Article
Chemistry, Multidisciplinary
Jing-Yang Zhang, Fang -Man Chen, Rong Liu, Jia-Qi Luo, Yong-Cong Huang, Na Shu, Sui-Juan Zheng, Dan Shao, Kam W. Leong, Jin-Zhi Du
Summary: This study identified that the use of the toll-like receptor 7/8 agonist resiquimod (R848) can promote macrophage phagocytosis and reprogram them into an anti-tumoral phenotype. The combination with the anti-SIRP alpha antibody (aSIRP alpha) further enhances the pro-phagocytic effect. A nanoparticle-based delivery system was developed to deliver R848 and aSIRP alpha simultaneously, leading to improved macrophage-mediated cancer immunotherapy.
Article
Biochemistry & Molecular Biology
Hong-Bo Fan, Yuan Zou, Qing Han, Qian-Wang Zheng, Ying-Li Liu, Li-Qiong Guo, Jun-Fang Lin
Summary: CMIMP significantly enhanced the phagocytic ability of immune cells and increased bactericidal activity through increasing F-actin expression and cell size in a TLR4-NF-kappa B pathway dependent way.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Chemistry, Applied
Lingrong Wen, Yueming Jiang, Xuesong Zhou, Huimin Bi, Bao Yang
Summary: Soybean peptides are functional foods with health benefits, with their structure playing a key role in determining these benefits. By identifying peptide structure and evaluating immunomodulatory activity, soybean peptides were found to facilitate macrophage proliferation.
Review
Immunology
Siqi Chen, Seigmund W. T. Lai, Christine E. Brown, Mingye Feng
Summary: Macrophage phagocytosis has emerged as a pivotal therapeutic strategy in cancer treatment. Targeting phagocytosis checkpoint blockade and using nanoparticles are effective ways to enhance macrophage phagocytosis, while chimeric antigen receptor macrophages serve as a next-generation therapeutic modality to induce efficacious anti-tumor immune responses by linking innate and adaptive immunity.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Biophysics
Hyungjin Kim, Daniel Roth, Yasuhiro Isoe, Koichiro Hayashi, Chihiro Mochizuki, Markus Kalkum, Michihiro Nakamura
Summary: In the presence of FBS, cellular uptake of FNP-PEGs was significantly reduced; pre-coating with BSA and Hb enhanced the stealth function of PEGs; BSA and Hb may act as stealth enhancement proteins for FNP-PEGs in vitro.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2021)
Article
Immunology
Yanchun Wang, Xia Fan, Lin Du, Boyu Liu, Haihan Xiao, Yan Zhang, Yunqiang Wu, Fuli Liu, Yung-Fu Chang, Xiaokui Guo, Ping He
Summary: While SR-A1 aids in the uptake of Leptospira by macrophages, it may be utilized by the pathogen to modulate inflammatory responses and increase susceptibility to infection in the host.
EMERGING MICROBES & INFECTIONS
(2021)
Article
Chemistry, Multidisciplinary
Hui Yang, Chang Liu, Yanjun Wu, Meng Yuan, Jiarun Huang, Yuhan Xia, Qinjie Ling, Peter R. Hoffmann, Zhi Huang, Tianfeng Chen
Summary: This study develops a translational nanosystem using D-mannose modified selenium nanoparticles to inhibit the occurrence and development of atherosclerosis, and demonstrates strong anti-inflammatory effects.
Article
Microbiology
Yan Liu, Yang Zhang, Xi Zhao, Weilai Lu, Yuxin Zhong, Yu V. Fu
Summary: Cryptococcus neoformans is a fungal pathogen that causes nearly half a million deaths worldwide each year. The polysaccharide capsule produced by C. neoformans is one of its main virulence factors and disrupts the immune response of the host. This study demonstrates that the antifungal peptide SP1 can disrupt the polysaccharide capsule of C. neoformans and enhance its clearance by host immune cells. SP1 also shows activity against cryptococcal biofilms. These findings suggest the potential of SP1 as a drug candidate for the treatment of cryptococcosis.
MICROBIOLOGY SPECTRUM
(2023)
Article
Dentistry, Oral Surgery & Medicine
John A. Pizarek, Nicholas G. Fischer, Conrado Aparicio
Summary: The study synthesized an IL-23R antagonist peptide nanocoating on titanium surfaces to reduce inflammation around dental implants and promote soft tissue healing.
Article
Engineering, Environmental
Xiali Liu, Huasheng Zhang, Huiqiang Ma, Jiameng Gong, Kaifeng Huang, Lu Wang, Yuting Ji, Liya Sun, Xiaoyu Wang, Li Li, Lei Shen, Shan-Yu Fung, Hong Yang
Summary: Asthma is a complex respiratory disease categorized into eosinophilic and neutrophilic asthma. While glucocorticoid therapy is effective for eosinophilic asthma, there is no effective treatment for neutrophilic asthma. Studies have shown the involvement of M1 macrophages in the pathophysiology of neutrophilic asthma.
CHEMICAL ENGINEERING JOURNAL
(2023)
Article
Materials Science, Biomaterials
Emerson L. Grey, Jazalle McClendon, Joshita Suresh, Scott Alper, William J. Janssen, Stephanie J. Bryant
Summary: Polymeric microparticles formed by a thiol-Michael addition step-growth polymerization reaction were studied for their potential in targeting macrophages. The size of the particles, treatment time, and particle chemistry were found to significantly affect their uptake by macrophages. Amide-terminated particles were non-inflammatory, while carboxyl- and thiol-terminated particles induced inflammation and cytokine production. A lung-specific application of amide-terminated particles was also explored, showing efficient uptake without causing inflammation in vitro and in vivo.
ACS BIOMATERIALS SCIENCE & ENGINEERING
(2023)
Article
Biochemistry & Molecular Biology
Colin Shortridge, Ehsan Akbari Fakhrabadi, Leah M. Wuescher, Randall G. Worth, Matthew W. Liberatore, Eda Yildirim-Ayan
Summary: The study investigated the effects of the digestive inflammatory environment on scaffold degradation, prevention of premature degradation using genipin crosslinking, and how genipin crosslinking affects IL-4 release and differentiation of naive macrophages. The results showed that the digestive inflammatory environment led to increased protein release from the scaffold, which was improved through genipin crosslinking. The optimal genipin concentration improved IL-4 release, cell viability, mechanical strength, scaffold porosity, and immunomodulation studies, but also decreased the immunomodulatory capacity of the scaffold.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Food Science & Technology
Hongyin Zhang, Fengqin Xiao, Jia Li, Rongxin Han, Guangzhe Li, Zhiqiang Wan, Shuai Shao, Daqing Zhao, Mingming Yan
Summary: This study aimed to investigate the protective effect of Semen Ziziphi Spinosae protein (SZSP) on immunosuppressed mice and its inhibitory effect on immune-stimulated RAW264.7 cells. The results showed that SZSP significantly improved immunomodulatory secretion and promoted NK-cell killing activity. Additionally, SZSP was found to regulate CD4+ and CD8+ secretion and inhibit damage induced by cyclophosphamide. It also inhibited the secretion of certain molecules and reduced the phosphorylation expression of specific proteins in lipopolysaccharide-stimulated cells. Therefore, SZSP has an immunoregulatory effect and may be a favorable candidate for functional food.
NPJ SCIENCE OF FOOD
(2023)
Article
Chemistry, Multidisciplinary
Yasmin Arezki, Francois Delalande, Christine Schaeffer-Reiss, Sarah Cianferani, Mickael Rapp, Luc Lebeau, Francoise Pons, Carole Ronzani
Summary: This study reported the first quantitative proteomic analysis of the protein corona formed on carbon dots (CDs) with different surface charge properties, showing that the surface charge properties of CDs influence protein corona composition, which in turn affects CD uptake and viability in macrophages.
Article
Engineering, Biomedical
Qingsheng Liu, Alan Chiu, Longhai Wang, Duo An, Wenchen Li, Esther Y. Chen, Yu Zhang, Yehudah Pardo, Sean P. McDonough, Lingyun Liu, Wendy F. Liu, Jing Chen, Minglin Ma
Article
Multidisciplinary Sciences
Vijaykumar S. Meli, Hamza Atcha, Praveen Krishna Veerasubramanian, Raji R. Nagalla, Thuy U. Luu, Esther Y. Chen, Christian F. Guerrero-Juarez, Kosuke Yamaga, William Pandori, Jessica Y. Hsieh, Timothy L. Downing, David A. Fruman, Melissa B. Lodoen, Maksim Plikus, Wenqi Wang, Wendy F. Liu
Article
Multidisciplinary Sciences
Hamza Atcha, Amit Jairaman, Jesse R. Holt, Vijaykumar S. Meli, Raji R. Nagalla, Praveen Krishna Veerasubramanian, Kyle T. Brumm, Huy E. Lim, Shivashankar Othy, Michael D. Cahalan, Medha M. Pathak, Wendy F. Liu
Summary: Studies reveal that Piezo1 serves as a mechanosensor of stiffness in macrophages, influencing their polarization responses.
NATURE COMMUNICATIONS
(2021)
Article
Immunology
Hamza Atcha, Vijaykumar S. Meli, Chase T. Davis, Kyle T. Brumm, Sara Anis, Jessica Chin, Kevin Jiang, Medha M. Pathak, Wendy F. Liu
Summary: The study demonstrates that both cyclic and static stretch influence macrophage morphology and responses to different stimuli. The integrin CD11b and ion channel Piezo1 may play a role in mechanotransduction in macrophages.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Sara Heedy, Juviarelli J. Pineda, Vijaykumar S. Meli, Szu-Wen Wang, Albert F. Yee
Summary: This study presents a bioinspired approach to enhance the stiffness and strength of biopolymer hydrogels and films through nanoscale architecture and templated microstructure. The results show that the nanopillar templating strategy improves the mechanical performance of chitosan hydrogels, surpassing the strength of unstructured hydrogels and exhibiting comparable performance to other stiff, strong hydrogels and natural tissues.
Article
Engineering, Biomedical
Praveen Krishna Veerasubramanian, Hanjuan Shao, Vijaykumar S. Meli, Tri Andrew Q. Phan, Thuy U. Luu, Wendy F. Liu, Timothy L. Downing
Summary: Macrophages are able to finely tune their function in response to the microenvironment through changes in cell morphology and epigenetic reprogramming. A cytoskeleton-dependent Src-H3 acetylation axis has been revealed to be responsible for inflammation-associated histone hyperacetylation in macrophages.
Editorial Material
Biochemistry & Molecular Biology
Bhumika Shokeen, Prabhat K. Purbey, Vijaykumar S. Meli
FRONTIERS IN MOLECULAR BIOSCIENCES
(2022)
Article
Materials Science, Biomaterials
Nina Butkovich, Jo Anne Tucker, Aaron Ramirez, Enya Li, Vijaykumar S. Meli, Edward L. Nelson, Szu-Wen Wang
Summary: The use of nanoparticles in cancer vaccines plays an important role in activating both mDCs and pDCs for antigen presentation, which has often been overlooked in the past. The study finds that nanoparticle-encapsulated TLR9 agonists activate mDCs, while both nanoparticle and individually tested constructs activate pDCs, with CpG1826 inducing pDC cytokine production. Furthermore, factors secreted from pDCs stimulated with a vaccine formulation comprising peptide antigen and CpG1826 enhance mDC antigen display.
BIOMATERIALS SCIENCE
(2023)
Review
Biochemistry & Molecular Biology
Vijaykumar S. Meli, Praveen Krishna Veerasubramanian, Timothy L. Downing, Wenqi Wang, Wendy F. Liu
Summary: Innate immune cells are crucial for eliminating foreign agents and cellular debris. Their ability to perceive and respond to biochemical and mechanical cues from the microenvironment plays a significant role in determining their behavior. Recent studies have shown the involvement of mechanosensitive proteins YAP and TAZ in inflammation and immunity, and this review focuses on understanding their role and therapeutic potential in inflammatory diseases.
Article
Biochemical Research Methods
Natalie J. Wu-Woods, Jacob T. Barlow, Florian Trigodet, Dustin G. Shaw, Anna E. Romano, Bana Jabri, A. Murat Eren, Rustem F. Ismagilov
Summary: This study introduces a microbial-enrichment method (MEM) that enables the removal of host DNA in human intestinal biopsies and allows for the characterization of low-abundance microbial taxa. The method provides deeper insights into the high-throughput characterization of the gut microbiome and demonstrates the construction of metagenome-assembled genomes from human intestinal biopsies.
Article
Orthopedics
Ryan P. Donahue, Jarrett M. Link, Vijaykumar S. Meli, Jerry C. Hu, Wendy F. Liu, Kyriacos A. Athanasiou
Summary: This study showed that the stiffness of neocartilage can affect the behavior of macrophages, and the use of bioactive factors can protect neocartilage from macrophage-induced degradation.
Article
Chemistry, Multidisciplinary
Junhan Zhou, Vijaykumar S. Meli, Esther Yu-Tin Chen, Rohan Kapre, Raji Nagalla, Wenwu Xiao, Alexander D. Borowsky, Kit S. Lam, Wendy F. Liu, Angelique Y. Louie
Summary: In the tumor micro-environment, tumor associated macrophages (TAMs) play a complex role in cancer pathogenesis. In this study, we investigate the potential of sulfated-dextran coated iron oxide nanoparticles (SDIO) as MRI imaging probes to label M2-polarized cells. We found that uptake of SDIO was positively correlated with the degree of sulfation, which highlights the potential of SDIO as a valuable addition to imaging probes for TAMs.
Article
Materials Science, Biomaterials
Vijaykumar S. Meli, Ryan P. Donahue, Jarrett M. Link, Jerry C. Hu, Kyriacos A. Athanasiou, Wendy F. Liu
Summary: This study establishes the use of porcine macrophages in biomaterial testing and reveals a stiffness-dependent effect on biomaterial-induced giant cell formation.
BIOMATERIALS SCIENCE
(2021)