Article
Oncology
Moon Nyeo Park, Hee Won Jeon, Md Ataur Rahman, Se Sun Park, Se Yun Jeong, Ki Hyun Kim, Sung-Hoon Kim, Woojin Kim, Bonglee Kim
Summary: The study reveals the anticancer effect of dragon's blood in acute myeloid leukemia by inducing apoptosis and modulating protein expression. It also highlights the role of reactive oxygen species and miR-216b in this process.
FRONTIERS IN ONCOLOGY
(2022)
Article
Oncology
Andrew J. Robinson, Sara Davies, Richard L. Darley, Alex Tonks
Summary: In AML patients, over 60% exhibit constitutive activation of NOX2 and increased ROS production. This leads to increased glucose uptake and proliferation in AML cells, as well as changes in carbohydrate metabolism. Metabolomic profiling revealed significant ROS-specific metabolic alterations in sphingolipid metabolism, fatty acid oxidation, purine metabolism, amino acid homeostasis, and glycolysis, providing further evidence of ROS rewiring metabolism in AML.
FRONTIERS IN ONCOLOGY
(2021)
Article
Oncology
Li Li, Weidong Zhao
Summary: This study discovered a feedback loop involving TPTEP1/JNK/c-JUN/miR-1303 axis in AML cells, suggesting TPTEP1 and miR-1303 as potential targets for developing therapeutic strategies for AML patients.
CANCER CELL INTERNATIONAL
(2021)
Article
Nanoscience & Nanotechnology
Jun Dou, Luoyang Li, Mei Guo, Feng Mei, Danfeng Zheng, Hui Xu, Rui Xue, Xueyang Bao, Fengshu Zhao, Yu Zhang
Summary: The study found that nanozyme-like Fe3O4 nanoparticles (IONPs) combined with cytosine arabinoside (Ara-C) effectively reduced the AML burden in mice engrafted with leukemia stem cells (LSCs) and extended mouse survival by increasing LSC's reactive oxygen species (ROS) levels to induce LSC apoptosis. The results suggest that targeting LSCs with IONPs plus Ara-C could control AML relapse.
INTERNATIONAL JOURNAL OF NANOMEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Zhengqi Wang, Tian Mi, Heath L. Bradley, Jonathan Metts, Himalee Sabnis, Wandi Zhu, Jack Arbiser, Kevin D. Bunting
Summary: Recent studies have shown that combining pimozide with mTOR inhibitors and BH3 mimetics is effective in AML cells. In Flt3-ITD+ cells, pimozide combined with IB can act through the Drp1-p38-ROS pathway. Overall, rational combination therapies show promise for improving responses in AML patients.
Article
Engineering, Biomedical
Yuxiang Sun, Xin Liu, Lei Wang, Li Xu, Kunliang Liu, Lei Xu, Fangfang Shi, Yu Zhang, Ning Gu, Fei Xiong
Summary: The SOD-like activity of CeO2 nanoparticles is inactivated by high oxidative stress. However, Au@Ce NPs, assembled by Au NPs and Ce NPs, exhibited high-performance SOD mimetic enzyme activity even under oxidative conditions. The enhanced localized surface plasmon resonance of nano-Au supported Ce NPs enables the maintenance of Ce3+/Ce4+ stability and SOD-like activity. Au@Ce NPs effectively scavenged ROS in AML cells, thereby inhibiting cell proliferation and cell cycle progression.
BIOACTIVE MATERIALS
(2022)
Article
Chemistry, Multidisciplinary
Fei Kong, Huiyuan Bai, Ming Ma, Chen Wang, Haiyan Xu, Ning Gu, Yu Zhang
Summary: The utilization of a composite nanozyme to target and treat acute myeloid leukemia represents a potential therapeutic approach, alleviating the toxic side effects of chemotherapy and improving treatment efficacy.
Review
Immunology
Sarah Weber, Anastasia Parmon, Nina Kurrle, Frank Schnuetgen, Hubert Serve
Summary: MDS and AML patients often experience systemic iron overload issues, iron is closely associated with the pathogenesis and potential treatment strategies of the diseases, imbalances in iron homeostasis may lead to various cell death.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Oncology
Assunta Adamo, Paulynn Chin, Peter Keane, Salam A. Assi, Sandeep Potluri, Sophie G. Kellaway, Daniel Coleman, Luke Ames, Anetta Ptasinska, H. Ruud Delwel, Peter N. Cockerill, Constanze Bonifer
Summary: This study defines a refined core gene regulatory network (GRN) common to all patients with CEBPA-double mutant (CEBPA(N/C)) AML by integrating gene expression, open chromatin, ChIP, and promoter-capture Hi-C data. Mutations in CEBPA disrupt the structure of a major regulator of myelopoiesis, and important network nodes are linked to the specific differentiation trajectory in AML.
Article
Biochemistry & Molecular Biology
Sylwester Glowacki, Ewelina Synowiec, Marzena Szwed, Monika Toma, Tomasz Skorski, Tomasz Sliwinski
Summary: Chronic myeloid leukemia (CML) is caused by the BCR-ABL1 protein, inducing oxidative stress and leading to resistance to imatinib (IM). Research shows increased ROS accumulation in BCR-ABL1 positive cells, heightened DNA damage in IM-sensitive cells, and alterations in antioxidant enzyme activity and mitochondrial potential in IM-resistant cells.
Article
Oncology
Muhammed Burak Demircan, Tina M. Schnoeder, Peter C. Mgbecheta, Katrin Schroeder, Frank-D Boehmer, Florian H. Heidel
Summary: Oxidative stress has been found to be associated with cancer initiation and progression. Recent studies have shown the potential therapeutic role of ROS modulation in various cancers, including acute myeloid leukemia (AML). A detailed understanding of the complex machinery regulating ROS in cancer is essential for defining potential therapeutic applications.
JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Joana Jorge, Nisa Magalhaes, Raquel Alves, Beatriz Lapa, Ana Cristina Goncalves, Ana Bela Sarmento-Ribeiro
Summary: This study evaluated the effect of Brusatol on in vitro models of acute lymphoblastic leukemia (ALL). Brusatol decreased metabolic activity in ALL cell lines, induced cell cycle arrest and cell death, and caused oxidative stress imbalance and mitochondrial disruption.
Article
Biochemistry & Molecular Biology
Bing Long, Yongli Shan, Yanling Sun, Tianyu Wang, Xudong Li, Ke Huang, Wenwen Zhang, Yi He, Ruijuan Wen, Yuhang Li, Yuchan Mai, Yashu Feng, Tian Zhang, Baoqiang Kang, Cong Zhang, Yanling Zhu, Jiaming Gu, Jiajun Liu, Xiangzhong Zhang, Guangjin Pan
Summary: Vitamin C significantly enhances the therapeutic effect of DZNep on leukemia cells, reducing drug resistance.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2022)
Editorial Material
Cell & Tissue Engineering
Malini Gupta, Britta Will
Summary: Adaptive aberrant gene regulation is a hallmark of malignant growth and therapy resistance in acute myeloid leukemia (AML). In this study, Eagle et al. identified oncogenic enhancer-driven overexpression of selenophosphate synthetase 2 (SEPHS2) as a targeted opportunity for mitigating malignant cell growth in AML.
Article
Pharmacology & Pharmacy
Yuta Semba, Shintaro Yamamoto, Shunsuke Takahashi, Takahisa Shinomiya, Yukitoshi Nagahara
Summary: Masitinib induces apoptosis in c-kit-negative HepG2 cells by targeting the redox balance through the JNK pathway, demonstrating its anticancer effects.
EUROPEAN JOURNAL OF PHARMACOLOGY
(2022)
Article
Oncology
Hibah Ali Almasmoum, Blessing Airhihen, Claire Seedhouse, Gerlof Sebastiaan Winkler
Summary: The study suggests that mutations in BTG1 may contribute to malignant transformation and tumor cell proliferation by interfering with its anti-proliferative activity and ability to interact with CNOT7 and CNOT8.
LEUKEMIA & LYMPHOMA
(2021)
Article
Microbiology
Jamie L. Thompson, Angela Downie Ruiz Velasco, Alice Cardall, Rebecca Tarbox, Jaineeta Richardson, Gemma Clarke, Michelle Lister, Hannah C. Howson-Wells, Vicki M. Fleming, Manjinder Khakh, Tim Sloan, Nichola Duckworth, Sarah Walsh, Chris Denning, C. Patrick McClure, Andrew V. Benest, Claire H. Seedhouse
Summary: The study evaluated alternative commercially available kits for SARS-CoV-2 genome detection and found that using heat inactivation in an extraction-free protocol could reduce the time from sample receipt to test outcome. The results demonstrate that sample treatment can significantly impact the performance of diagnostic kits, with different effects depending on the kit used.
JOURNAL OF MEDICAL MICROBIOLOGY
(2021)
Article
Chemistry, Applied
Fong-Kai Lee, Premanand Krishnan, Azira Muhamad, Yun-Yee Low, Toh-Seok Kam, Kang-Nee Ting, Kuan-Hon Lim
Summary: A concise synthesis of 1,4-diarylbutanoid-phenethylamine alkaloids schwarzinicines A and B, recently isolated from Ficus schwarzii, was reported. Key steps included Claisen condensation and reductive amination to assemble the core skeleton of the target compounds, with overall synthetic yields of 9.1% and 3.5% for compounds 1 and 2, respectively. Synthetic derivatives showed comparable vasorelaxant effects on rat isolated aortic rings as natural schwarzinicine A, indicating chirality at C-2 did not influence the observed vasorelaxant effects.
NATURAL PRODUCT RESEARCH
(2022)
Article
Plant Sciences
Zi-Yang Chan, Premanand Krishnan, Sayed Mohammadhossein Modaresi, Ling-Wei Hii, Chun-Wai Mai, Wei-Meng Lim, Chee-Onn Leong, Yun-Yee Low, Soon-Kit Wong, Kien-Thai Yong, Alyssa Zi-Xin Leong, Mei-Kee Lee, Kang-Nee Ting, Kuan-Hon Lim
Summary: Seven new tropane alkaloids were isolated from the leaves and bark of Pellacalyx saccardianus, with two showing cytotoxicity against human pancreatic cancer cells and three inducing smooth muscle relaxation in isolated rat tracheal rings.
JOURNAL OF NATURAL PRODUCTS
(2021)
Article
Biochemistry & Molecular Biology
Eun-Seon Cho, Premanand Krishnan, Hwei-San Loh, Janet M. Daly, Chee-Onn Leong, Chun-Wai Mai, Yun-Yee Low, Kien-Thai Yong, Kuan-Hon Lim
Summary: Four new cucurbitacins, petiolaticins A-D, were isolated from the bark and leaves of Elaeocarpus petiolatus. Petiolaticin A exhibited potent cytotoxicity against various cancer cell lines, while petiolaticin D showed significant antiviral properties against a pseudotyped virus. These findings indicate the potential of petiolaticins A and D as anticancer and antiviral agents, respectively, warranting further investigation.
Article
Plant Sciences
Zi-Yang Chan, Premanand Krishnan, Ling -Wei Hii, Chun-Wai Mai, Chee-Onn Leong, Yun-Yee Low, Soon-Kit Wong, Kang-Nee Ting, Kien -Thai Yong, Kuan-Hon Lim
Summary: A variety of compounds with antitumor activity were isolated from the leaves of Saccardianus pycnolobium, including diarylheptanoid-phenylpropanoid adducts and diarylheptanoid alkaloids with different core structures. Among them, (+/-)-2 and 6 showed selective cytotoxicity towards pancreatic cancer cell lines.
PHYTOCHEMISTRY LETTERS
(2021)
Article
Oncology
Rachael Nicholson, Ana Catarina Menezes, Aleksandra Azevedo, Adam Leckenby, Sara Davies, Claire Seedhouse, Amanda Gilkes, Steve Knapper, Alex Tonks, Richard L. Darley
Summary: This study found that PKC epsilon is significantly overexpressed in acute myeloid leukemia (AML) and is associated with reduced complete remission induction and disease-free survival. Additionally, PKC epsilon overexpression confers resistance to the chemotherapeutic agent daunorubicin by upregulating the expression of the efflux pump P-GP.
FRONTIERS IN ONCOLOGY
(2022)
Article
Multidisciplinary Sciences
Harry H. Jenkins, Ana A. Tellechea Lopez, Francesco Saverio Tarantini, Hannah Tomlin, Danielle Scales, I-Ning Lee, Siyu Wu, Ralph Hyde, Katarzyna Lis-Slimak, Timothy Byaruhanga, Jamie L. Thompson, Sara Pijuan-Galito, Lara Doolan, Kazuyo Kaneko, Penny Gwynne, Caroline Reffin, Emily Park, Jayasree Dey, Jack Hill, Asta Arendt-Tranholm, Amy Stroud, Moira Petrie, Chris Denning, Andrew Benest, Claire Seedhouse
Summary: Polymerase chain reaction (PCR) has been proven to be the gold-standard for SARS-CoV-2 detection. Performing a non-invasive one-step RT-qPCR assay directly on saliva specimens simplifies sample collection, reduces processing time and costs. The assay demonstrates good sensitivity and concordance.
SCIENTIFIC REPORTS
(2022)
Article
Chemistry, Multidisciplinary
Haneen Abuzaid, Salah Abdelrazig, Lenny Ferreira, Hilary M. Collins, Dong-Hyun Kim, Kuan-Hon Lim, Toh-Seok Kam, Lyudmila Turyanska, Tracey D. Bradshaw
Summary: The development of an APO protein-based delivery system for the anti-tumor alkaloid JAa enhances tumor-targeting and reduces systemic toxicity in breast cancer cells.
Article
Plant Sciences
Yi-Sheng Tan, Soon-Kit Wong, Kien-Thai Yong, Kuan-Hon Lim, Siew-Huah Lim, Yun-Yee Low
Summary: Eugeniifoline (1), a pentacyclic indole alkaloid with a five-membered ring E, was isolated for the first time as a natural product from the stem-bark extract of Leuconotis eugeniifolia. Previously reported as a synthetic product with the configuration at C-21 as S (1a), the configuration at C-21 was revised to R based on various analyses.
JOURNAL OF NATURAL PRODUCTS
(2023)
Article
Biochemistry & Molecular Biology
Jenna R. James, Johnathan Curd, Jennifer C. Ashworth, Mays Abuhantash, Martin Grundy, Claire H. Seedhouse, Kenton P. Arkill, Amanda J. Wright, Catherine L. R. Merry, Alexander Thompson
Summary: A 3D model of the bone marrow niche has been developed using a synthetic peptide hydrogel (SAPH) to screen FDA-approved drugs for acute myeloid leukemia (AML). The optimized stiffness of SAPH affects AML cell proliferation and Salinomycin shows efficacy in AML patient samples. Vidofludimus is not sensitive to AML cells in the hydrogel models.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Chemistry, Medicinal
Jin-Ning Chu, Premanand Krishnan, Kuan-Hon Lim
Summary: Sarcandra glabra is a perennial evergreen herb with high ornamental value and rich traditional medicinal uses in China. Modern pharmacological studies have revealed its diverse biological activities. Over 400 compounds have been isolated and characterized, some with unique structures.
NATURAL PRODUCTS AND BIOPROSPECTING
(2023)
Article
Biochemical Research Methods
Francesco Saverio Tarantini, Siyu Wu, Harry Jenkins, Ana Tellechea Lopez, Hannah Tomlin, Ralph Hyde, Katarzyna Lis-Slimak, Jamie Louise Thompson, Sara Pijuan-Galito, Danielle Scales, Kazuyo Kaneko, Jayasree Dey, Emily Park, Jack Hill, I-Ning Lee, Lara Doolan, Asta Arendt-Tranholm, Chris Denning, Claire Seedhouse, Andrew Benest
Summary: Since mid-2020, there have been difficulties in standardizing and administering nasopharyngeal swabs, leading to testing fatigue and reduced confidence in results. On the other hand, saliva-based PCR testing is gaining momentum as a non-invasive, highly sensitive, and accurate method.
METHODS AND PROTOCOLS
(2022)
Article
Materials Science, Paper & Wood
Jing Ying Yap, Ching Lik Hii, Sze Pheng Ong, Kuan Hon Lim, Faridah Abas, Kar Yong Pin
Summary: The significant increase in dengue cases globally has led to the exploration of alternative treatments, with papaya leaves extract (PLE) being used successfully due to its carpaine content that raises platelet count. Studies have shown that young papaya leaves contain significantly higher levels of carpaine compared to old leaves and stalks, and blending treatment can further enhance carpaine extraction.
JOURNAL OF BIORESOURCES AND BIOPRODUCTS
(2021)