Article
Biotechnology & Applied Microbiology
Peilin Li, Daisuke Miyamoto, Yu Huang, Tomohiko Adachi, Masaaki Hidaka, Takanobu Hara, Akihiko Soyama, Hajime Matsushima, Hajime Imamura, Kengo Kanetaka, Weili Gu, Susumu Eguchi
Summary: In this study, a novel in vitro model combining human mature hepatocytes (hMHs) with three-dimensional intrahepatic bile ducts (BDs) was established. The BDs, generated from chemically induced human liver progenitor cells (hCLiPs) derived from hMHs, expressed typical biliary markers and were able to transport bile-like substances. This model provides a valuable tool for studying cholangiocyte function, drug screening, and therapeutic modulation.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Gastroenterology & Hepatology
Ehud Zigmond, Britta Franziska Zecher, Anna-Lena Bartels, Tomer Ziv-Baran, Thomas Roesch, Guido Schachschal, Ansgar W. Lohse, Hanno Ehlken, Christoph Schramm
Summary: Primary sclerosing cholangitis (PSC) is characterized by chronic inflammation of the biliary mucosa and colonization of the bile ducts with bacteria. This study found that the presence of Enterococci and Fungobilia in the bile ducts of PSC patients was associated with disease progression, highlighting the importance of microbiota-mucosal interaction in the pathogenesis of PSC.
CLINICAL GASTROENTEROLOGY AND HEPATOLOGY
(2023)
Article
Medicine, General & Internal
Mark Ly, Ngee-Soon Lau, Catriona Mckenzie, James G. Kench, Doruk Seyfi, Avik Majumdar, Ken Liu, Geoffrey Mccaughan, Michael Crawford, Carlo Pulitano
Summary: Histological assessment of bile duct injury is limited in predicting post-transplant biliary strictures.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Environmental Sciences
Lingyu Hu, Xiaofang Wang, Zhiwei Bao, Qihao Xu, Mingrong Qian, Yuanxiang Jin
Summary: The fungicide prothioconazole and its metabolite prothioconazole-desthio have harmful effects on the liver-gut axis of mice, altering bile acid metabolism, intestinal microbiota composition, and gut barrier function.
Article
Microbiology
Snehal N. Chaudhari, James N. Luo, David A. Harris, Hassan Aliakbarian, Lina Yao, Donggi Paik, Renuka Subramaniam, Arijit A. Adhikari, Ashley H. Vernon, Ayse Kilic, Scott T. Weiss, Jun R. Huh, Eric G. Sheu, A. Sloan Devlin
Summary: Bariatric surgery is the most effective treatment for type 2 diabetes, associated with changes in gut metabolites. Activation of the vitamin D receptor post-surgery leads to increased production of cholic acid-7-sulfate (CA7S), facilitating selective transport across the gut epithelium. Additionally, the microbiome-dependent pathway plays a crucial role in connecting a microbial metabolite with the improvement of diabetic phenotypes through CA7S synthesis and GLP-1 secretion.
CELL HOST & MICROBE
(2021)
Article
Pharmacology & Pharmacy
Zahra Barmoudeh, Mohamad Hassan Fouani, Zahra Moslemi, Mahdokht Azizi, Amir Hossein Doustimotlagh, Hassan Bardania
Summary: The therapeutic effectiveness of co-loaded liposomes of metformin (Met) and melatonin (Mel) was investigated on cholestasis induced by bile duct ligation (BDL) in male rats. The results showed that liposomal form of Mel and Met had a stronger effect in reducing inflammation and bile duct proliferation compared to their free forms. Moreover, liposomal treatment resulted in lower levels of aspartate aminotransferase (AST) and bilirubin, and downregulation of inflammatory cytokine genes TNF-α and IL-1. Overall, liposomal co-administration of Met and Mel had a better therapeutic effect on liver injury by improving histological changes, reducing biochemical markers, and favoring oxidant-antioxidant balance.
NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY
(2023)
Article
Surgery
Vincenzo Carollo, Fabrizio di Francesco, Calogero Ricotta, Pasquale Bonsignore, Roberto Miraglia, Jean de Ville de Goyet
Summary: This retrospective study analyzed data from 40 donors for liver lateral section graft transplantation, finding that preoperative imaging and detailed anatomical studies are crucial for determining surgical strategy, with significant impact of hepatic arterial or biliary variations on decision-making for liver division during transplant surgery.
INTERNATIONAL JOURNAL OF SURGERY
(2021)
Article
Surgery
Seong Ji Choi, Yoon Jai Hoon, Dong Hee Koh, Hang Lak Lee, Dae Won Jun, Ho Soon Choi
Summary: This study retrospectively analyzed 362 patients who underwent ERCP for CBD stones and found that risk factors such as low cystic duct insertion, distal CBD angulation, larger CBD diameter, more ERCP sessions, and longer cannulation time were associated with symptomatic recurrence of CBD stones. Patients with these risk factors, particularly low cystic duct insertion, should be followed more closely to prevent recurrence.
SURGICAL ENDOSCOPY AND OTHER INTERVENTIONAL TECHNIQUES
(2022)
Article
Agriculture, Multidisciplinary
Zunji Shi, Gui Chen, Zheng Cao, Fang Wu, Hehua Lei, Chuan Chen, Yuchen Song, Caixiang Liu, Jinquan Li, Jinlin Zhou, Yujing Lu, Limin Zhang
Summary: This study evaluated the functional effects of bile acids (BAs) and gut microbiota on sucralose consumption-induced nonalcoholic fatty liver disease (NAFLD) in mice. Results showed that sucralose consumption increased the abundance of intestinal genera Bacteroides and Clostridium, leading to elevated hepatic deoxycholic acid (DCA) levels which inhibited hepatic gene expression and contributed to NAFLD. Dietary supplements with fructo-oligosaccharide or metformin restored the gut microbiota and DCA levels, thereby ameliorating NAFLD in sucralose-consuming mice. These findings highlighted the important role of gut microbiota and DCA in the development of NAFLD induced by sucralose.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Oncology
Kari Hemminki, Kristina Sundquist, Jan Sundquist, Asta Foersti, Vaclav Liska, Akseli Hemminki, Xinjun Li
Summary: Liver cancer, including hepatocellular carcinoma, gallbladder cancer, and bile duct cancer, is influenced by various risk factors such as alcohol, viral hepatitis, gallstones, infections, autoimmune diseases, and diabetes. This study demonstrates that the combination of these risk factors can account for at least 50% of the causes of these cancers, highlighting the significance of chronic inflammation as an underlying mechanism. The use of national hospital records in Sweden provides valuable insights into the potential comorbidities associated with hepatobiliary cancers.
Article
Medicine, Research & Experimental
Zhi Ma, Xiaoyong Xue, Jinzhao Bai, Yajie Cai, Xuejing Jin, Kexin Jia, Mingning Ding, Xiangjun Lyu, Xiaojiaoyang Li
Summary: Si-Wu-Tang (SWT) has been found to improve liver fibrosis by modulating the immune microenvironment. It repairs the imbalanced immune microenvironment by regulating the biological functions of different immune cells, especially macrophages, neutrophils, and CD8+ T cells. SWT inhibits the release of profibrotic cytokines and promotes the apoptosis of activated hepatic stellate cells. This research provides insights into the mechanisms of SWT's hepatoprotective activities and offers a novel therapeutic strategy for liver fibrosis.
BIOMEDICINE & PHARMACOTHERAPY
(2022)
Article
Multidisciplinary Sciences
Simona Hankeova, Jakub Salplachta, Noemi Van Hul, Michaela Kavkova, Afshan Iqbal, Tomas Zikmund, Jozef Kaiser, Emma R. Andersson
Summary: The technique described in the study, double resin casting micro-computed tomography (DUCT), involves injecting two different resins into the portal vein and bile duct, followed by tissue fixation, to visualize the three-dimensional structure of the liver. DUCT can be applied to postnatal and adult mouse liver and other tubular networks.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2021)
Article
Pharmacology & Pharmacy
Chang Ke, Jianlong Gao, Jiyuan Tu, Yan Wang, Yangxin Xiao, Yuan Wu, Yanju Liu, Zhongshi Zhou
Summary: This study aimed to investigate the protective effect of Ganfule capsule (GFL) on liver fibrosis and its possible mechanism. The cholestatic liver fibrosis model was prepared by bile duct ligation (BDL) in mice. The results showed that GFL could alleviate liver injury and fibrosis caused by BDL. Metabonomic analysis revealed metabolic disorder after surgery, which could be alleviated by GFL through regulation of glutamine metabolism and other pathways. GFL inhibited the activity of glutaminase 1 (GLS1) and the NF-kappa B pathway, leading to the alleviation of liver fibrosis.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Surgery
Mario Vilatoba, Mariana Chavez-Villa, Rodrigo Figueroa-Mendez, Ismael Dominguez-Rosado, Rodrigo Cruz-Martinez, Rafael P. Leal-Villalpando, Ignacio Garcia-Juarez, Miguel A. Mercado
Summary: By analyzing the outcomes of patients undergoing liver transplantation (LT) due to bile duct injury (BDI), it was found that although the postoperative mortality is high, LT remains the only possibility of a cure and has acceptable long-term outcomes. Early referral to a tertiary care center is crucial for avoiding long-term complications of BDI. Post-cholecystectomy BDI accounted for 3.4% of all LT in our center.
Article
Gastroenterology & Hepatology
Jens Mittler, Kenneth D. Chavin, Stefan Heinrich, Roman Kloeckner, Tim Zimmermann, Hauke Lang
Summary: This study demonstrated the feasibility and success of duct-to-duct biliary reconstruction in selected patients requiring surgical repair for isolated AS after DDLT. Patients who underwent this procedure had fully normalized liver function tests post-operatively and did not require any biliary interventions. Although there were cases of post-operative complications such as intraoperative cholangiosepsis and subhepatic abscess, there were no perioperative deaths.
JOURNAL OF GASTROINTESTINAL SURGERY
(2021)