Article
Environmental Sciences
Arijana Filipic, David Dobnik, Ion Gutierrez-Aguirre, Maja Ravnikar, Tamara Kosir, Spela Baebler, Alja Stern, Bojana Zegura, Martin Petkovsek, Matevz Dular, Miran Mozetic, Rok Zaplotnik, Gregor Primc
Summary: Water scarcity is a significant challenge we face today, and the inactivation of waterborne viruses is an important concern. This study successfully developed a device combining plasma and supercavitation, which demonstrated high efficiency in water decontamination. The device is environmentally friendly and provides contamination-free and safe water resources.
ENVIRONMENT INTERNATIONAL
(2023)
Article
Food Science & Technology
Branko Velebit, Lazar Milojevic, Tatjana Baltic, Nevena Grkovic, Sanjay Gummalla, Marina Velebit, Ines Skoko, Sandra Mojsova, Predrag Putnik
Summary: This study investigates the effectiveness of cold atmospheric plasma (CAP) in inactivating viruses on raspberries. CAP treatment successfully reduces the viral load in a short period of time without affecting the fruit quality. This provides an environmentally-friendly and cost-effective method for microbial decontamination in the raspberry industry.
INNOVATIVE FOOD SCIENCE & EMERGING TECHNOLOGIES
(2022)
Article
Microbiology
Arijana Filipic, David Dobnik, Magda Tusek Znidaric, Bojana Zegura, Alja Stern, Gregor Primc, Miran Mozetic, Maja Ravnikar, Jana Zel, Ion Gutierrez Aguirre
Summary: Water scarcity poses a major threat to human survival and quality of life, leading to an increased risk of infections from waterborne viruses. Cold atmospheric plasma (CAP) is emerging as an efficient, safe, and sustainable alternative for waterborne virus inactivation. In this study, CAP treatment successfully inactivated a resilient waterborne virus, PMMoV, without introducing any cytotoxic or genotoxic effects, showing promise for further examination as an alternative for treating potable and irrigation waters, as well as other sources of water, with a focus on inactivating various viruses, including enteric viruses.
FRONTIERS IN MICROBIOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Dayun Yan, Alisa Malyavko, Qihui Wang, Kostya (Ken) Ostrikov, Jonathan H. Sherman, Michael Keidar
Summary: Cold atmospheric plasma (CAP) is a partially ionized gas that can effectively induce cell death in a wide range of cell types and inactivate viruses. The cell death types triggered by CAP mainly include apoptosis, necrosis, and autophagy-associated cell death. The physical effects of CAP might provide potential mechanisms for targeting cancer cells.
Review
Multidisciplinary Sciences
Neha Kaushik, Sarmistha Mitra, Eun Jung Baek, Linh Nhat Nguyen, Pradeep Bhartiya, June Hyun Kim, Eun Ha Choi, Nagendra Kumar Kaushik
Summary: This review critically examines methods for generating reactive oxygen species (ROS) and explores their potential as a solution for virus deactivation. The study highlights the ability of ROS to directly eradicate pathogens through oxidative stress and indirectly through non-oxidative mechanisms. Additionally, the novel and environmentally friendly cold plasma delivery system for virus destruction is discussed in detail.
JOURNAL OF ADVANCED RESEARCH
(2023)
Review
Biochemistry & Molecular Biology
Darin Khumsupan, Shin-Ping Lin, Chang-Wei Hsieh, Shella Permatasari Santoso, Yu-Jou Chou, Kuan-Chen Hsieh, Hui-Wen Lin, Yuwen Ting, Kuan-Chen Cheng
Summary: The cost-effectiveness and efficiency of atmospheric cold plasma (ACP) technology has driven extensive research in its applications within the food industry. It has been found to be effective in food disinfection, modification, drying pre-treatment, nutrient extraction, active packaging, and food waste processing. Reactive oxygen and nitrogen species (RONS) play crucial roles in achieving the desired outcomes. This review article discusses the operations of ACP and summarizes its recent progress in food processing and safety, while also proposing current challenges and future prospects.
Article
Food Science & Technology
Manoharan Dharini, Stephen Jaspin, Rangarajan Jagan Mohan, Radhakrishnan Mahendran
Summary: This study investigates the impact of different plasma applications and their combination on milk, focusing on volatile component analysis. Plasma treatments altered the volatile nature of milk, with the combination treatment amplifying the volatiles produced. Combining two plasma treatments showed a positive synergistic effect in microbial reduction but increased milk oxidation. This study is the first to combine plasma bubbling and low-pressure plasma methods, providing insights into the impact and synergistic effects of plasma applications on milk through volatile compound, microbial, and oxidative analysis.
JOURNAL OF FOOD PROCESSING AND PRESERVATION
(2022)
Article
Food Science & Technology
Emine Ozen, Govindaraj Dev Kumar, Abhinav Mishra, Rakesh K. Singh
Summary: Atmospheric cold plasma (ACP) is a non-thermal technology that has the potential to inactivate microorganisms in foods. In this study, ACP was used to investigate the inactivation of E. coli K12, acid-adapted E. coli K12, and E. coli O157:H7 in apple cider. The results showed that ACP could effectively reduce the populations of these bacteria within a short processing time. The pH value of the cider was significantly affected by longer processing times. The gas type had different effects on the inactivation, and ozone was found to play a key role in the process. The findings provide insights into the use of ACP for decontaminating fruit juices.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2022)
Article
Food Science & Technology
Jiajie Hu, Weijiao Huang, Yihong Wang, Jing Jin, Yuwei Li, Jing Chen, Yan Zheng, Shanggui Deng
Summary: This study aimed to investigate the inactivation mechanism of atmospheric cold plasma (ACP) against Shewanella putrefaciens in both PBS and sterile shrimp juice (SSJ). The results showed that ACP treatment reduced cell density, cell viability, and biofilm formation activity. ACP cyclical treatment was more efficient than a one-time treatment. The damage to cell wall integrity, as well as cell membrane and DNA damage, were observed after ACP treatment. The study also found that ACP treatment could serve as a potential alternative to enhance microbial control in food processing.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2023)
Article
Multidisciplinary Sciences
Osvaldo Daniel Cortazar, Ana Megia-Macias, Sandra Moreno, Alejandro Brun, Eduardo Gomez-Casado
Summary: This study demonstrates the capability of Plasma Activated Media (PAM) to inactivate SARS-CoV-2 and PR8 H1N1 influenza virus without damaging healthy cells, acting through virus detachment and reduced replication. Furthermore, PAM treatment does not induce inflammation. These findings open up a new research avenue for the development of novel treatments for COVID-19, influenza, and other respiratory diseases using PAM.
SCIENTIFIC REPORTS
(2022)
Article
Food Science & Technology
Jichao Huang, Kairan Sheng, Yali Zhang, Mengmeng Song, Ahtisham Ali, Tianran Huang, Ming Huang
Summary: This study identified a new sterilization combination consisting of a germinant (AGFNa-1) and cold plasma treatment that effectively deactivated Bacillus licheniformis spores. The combination not only reduced the spore count, but also disrupted the inner membrane structure and inactivated the bacteria.
Article
Food Science & Technology
Kate Jenns, Hannah P. Sassi, Renwu Zhou, Patrick J. Cullen, Dee Carter, Anne Mai-Prochnow
Summary: This review evaluates the potential of cold atmospheric plasma (CAP) as a method for inactivating foodborne viruses. CAP has been shown to successfully inactivate foodborne viruses and its non-toxic, residue-free sanitation system for fresh foods makes it highly attractive to the industry. However, the lack of standard devices or protocols makes it difficult to translate these findings into meaningful applications.
TRENDS IN FOOD SCIENCE & TECHNOLOGY
(2022)
Article
Food Science & Technology
Yijie Zhao, Lele Shao, Lihong Jia, Bo Zou, Ruitong Dai, Xingmin Li, Fei Jia
Summary: This study investigated the effects of cold atmospheric plasma on Pseudomonas aeruginosa and its inactivation mechanism. The results showed that air-CAP had better inactivation capacity than nitrogen-CAP, and the mechanism involved damage to cell morphology, changes in membrane permeability, disruption of intracellular ionic equilibrium, and lipid peroxidation.
INNOVATIVE FOOD SCIENCE & EMERGING TECHNOLOGIES
(2022)
Article
Food Science & Technology
Wei Rao, Yeqing Li, Harleen Dhaliwal, Mengmeng Feng, Qisen Xiang, M. S. Roopesh, Daodong Pan, Lihui Du
Summary: Cold plasma technology, with its advantages in safety, efficiency, versatility, and environmentally friendly nature, has shown great potential in the application to low-moisture foods. It can effectively inactivate pathogenic microorganisms and degrade mycotoxins while minimal impact on food quality. Additionally, it has promising applications in seed germination, which could be significant to address the global food crisis. However, more systematic studies are needed for selected applications in the low-moisture foods industry.
FOOD ENGINEERING REVIEWS
(2023)
Review
Environmental Sciences
Jile Fu, Yiyi Xu, Eric J. Arts, Zhengyu Bai, Zhongwei Chen, Ying Zheng
Summary: The transmission of viral infections via aerosol is a serious threat to public health, and non-thermal plasma technology has gained interest for its highly effective disinfection and flexible operation.
Article
Acoustics
Arijana Filipic, Tadeja Lukezic, Katarina Bacnik, Maja Ravnikar, Meta Jeselnik, Tamara Kosir, Martin Petkovsek, Mojca Zupanc, Matevz Dular, Ion Gutierrez Aguirre
Summary: Waterborne plant viruses can cause significant damage to crops, but proper water treatment strategies can prevent their spread. Hydrodynamic cavitation (HC) is an environmentally friendly method for treating recycled water, which has shown promising results in decontamination.
ULTRASONICS SONOCHEMISTRY
(2022)
Article
Forestry
Anja Verbic, Katja Brencic, Gregor Primc, Marija Gorjanc
Summary: This study proposes two protocols for the green in situ synthesis of zinc oxide nanoparticles on cotton to develop sustainable cotton fabric with UV protection. The order of immersion of cotton fabric in reactive solutions affects the morphology of the nanoparticles and consequently the UV blocking abilities of the samples.
Article
Chemistry, Multidisciplinary
Alenka Vesel, Rok Zaplotnik, Gregor Primc, Domen Paul, Miran Mozetic
Summary: Carbon nanowalls are a promising material for electrochemical devices due to their properties such as chemical stability, electrical conductivity, and surface-to-mass ratio. This paper presents an alternative deposition technique using various polymers as solid precursors to synthesize carbon nanowalls on titanium substrates.
Article
Agronomy
Matej Holc, Peter Gselman, Gregor Primc, Alenka Vesel, Miran Mozetic, Nina Recek
Summary: In this study, it was found that treatment of alfalfa seeds with plasma improves their surface hydrophilicity, leading to potential benefits for cultivation and growth. The functionalization and etching of seed surface contribute to the regulation of seed wettability.
Article
Engineering, Mechanical
Tamara Kosir, Katja Fric, Arijana Filipic, Polona Kogovsek
Summary: Face coverings play an important role in preventing bacterial and viral transmission, especially during the global COVID-19 pandemic. The study found that most of the tested samples met the requirements for the national or European market in terms of bacterial filtration efficiency.
STROJNISKI VESTNIK-JOURNAL OF MECHANICAL ENGINEERING
(2022)
Article
Microbiology
Anja Pecman, Ian Adams, Ion Gutierrez-Aguirre, Adrian Fox, Neil Boonham, Maja Ravnikar, Denis Kutnjak
Summary: Nanopore sequencing, as a novel high-throughput sequencing technology, shows similar performance to Illumina sequencing in the detection of plant viruses and viroids, while offering the advantages of faster and more affordable diagnostics.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Materials Science, Multidisciplinary
Anja Verbic, Katja Brencic, Gregor Primc, Miran Mozetic, Marija Gorjanc
Summary: This study presents an eco-friendly protocol for improving the properties of PET fabric and synthesizing ZnO particles. The results showed that incorporating oxygen plasma treatment before immersing the fabric in each solution for ZnO synthesis produced PET fabric with the most uniform ZnO layer and the highest UV-blocking ability.
Article
Environmental Sciences
Olivera Maksimovic Carvalho Ferreira, Ziva Lengar, Zala Kogej, Katarina Bacnik, Irena Bajde, Mojca Milavec, Anze Zupanic, Natasa Mehle, Denis Kutnjak, Maja Ravnikar, Ion Gutierrez-Aguirre
Summary: The SARS-CoV-2 pandemic has accelerated the development of virus detection methods and overall approaches to epidemiology. Wastewater-based epidemiology has been employed to track the virus transmission dynamics. This article presents a retrospective view on the method development and implementation for a pilot monitoring in Slovenia. The study found that using Centricon filtration units coupled with Qiagen RNA extraction kit or direct RNA capture and extraction using a semi-automated kit from Promega produced the most optimal results out of the seven tested methods. The CDC's N1 and N2 assays with Fast Virus 1-mastermix were confirmed as the best performing assays among the four tested.
FOOD AND ENVIRONMENTAL VIROLOGY
(2022)
Article
Polymer Science
Olivija Plohl, Katja Fric, Arijana Filipic, Polona Kogovsek, Magda Tusek Znidaric, Lidija Fras Zemljic
Summary: This study evaluated the properties and antiviral activity of chitosan-based bioactive polymers as colloidal macromolecular solutions and explored their mechanisms of action.
Article
Biochemistry & Molecular Biology
Nina Recek, Rok Zaplotnik, Alenka Vesel, Gregor Primc, Peter Gselman, Miran Mozetic, Matej Holc
Summary: In this study, the effects of radio frequency oxygen plasma treatment on maize seeds were investigated. The results showed that plasma-treated seeds had a positive effect on plant yield. However, the differences were only partially significant. The presence of insect pests negatively affected the results in the first harvest year.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Acoustics
Mojca Zupanc, Jure Zevnik, Arijana Filipic, Ion Gutierrez-Aguirre, Meta Jeselnik, Tamara Kosir, Jernej Ortar, Matevz Dular, Martin Petkovsek
Summary: The study investigates the effects of hydrodynamic cavitation on the inactivation of SARS-CoV-2 surrogate bacteriophage phi6. The results show that hydrodynamic cavitation can significantly reduce the virus count at certain temperatures. This suggests that hydrodynamic cavitation has the potential to inactivate current and potential enveloped viruses in water at lower temperatures.
ULTRASONICS SONOCHEMISTRY
(2023)
Review
Chemistry, Physical
Domen Paul, Miran Mozetic, Rok Zaplotnik, Gregor Primc, Denis Donlagic, Alenka Vesel
Summary: This article reviews and discusses relevant data on the heterogeneous surface recombination of neutral oxygen atoms for various materials. The coefficients are determined through experiments with non-equilibrium oxygen plasma or its afterglow. Different experimental methods are examined and categorized, and numerical models for coefficient determination are also investigated. The article correlates the experimental parameters with reported coefficients, categorizes materials based on reported coefficients, and compares the measurements from the literature. It also discusses the scattering of results reported by different authors and provides possible explanations.
Article
Chemistry, Physical
Domen Paul, Miran Mozetic, Rok Zaplotnik, Jernej Ekar, Alenka Vesel, Gregor Primc, Denis Donlagic
Summary: Calorimetry is commonly used for plasma characterization, but its accuracy is tied to the recombination coefficient, which depends on surface effects. Surface effects also govern the kinetics in advanced methods such as atomic layer oxidation and functionalization. The recombination coefficient of oxidized cobalt surface was studied systematically, showing a monotonic increase with decreasing pressure and increasing temperature.
Article
Environmental Sciences
Arijana Filipic, Katja Fric, Maja Ravnikar, Polona Kogovsek
Summary: Due to the COVID-19 pandemic, there has been an influx of new materials and masks in the market. However, existing standards only specify bacterial filtration efficiency (BFE) testing and lack guidelines for determining viral filtration efficiency (VFE). This study evaluated different systems for determining VFE and found that the tested masks showed high VFE. Therefore, the VFE tests described in this study can be incorporated into existing mask testing standards.
INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH
(2022)
