Reversed paired-gRNA plasmid cloning strategy for efficient genome editing in Escherichia coli
Published 2020 View Full Article
- Home
- Publications
- Publication Search
- Publication Details
Title
Reversed paired-gRNA plasmid cloning strategy for efficient genome editing in Escherichia coli
Authors
Keywords
-
Journal
Microbial Cell Factories
Volume 19, Issue 1, Pages -
Publisher
Springer Science and Business Media LLC
Online
2020-03-10
DOI
10.1186/s12934-020-01321-4
References
Ask authors/readers for more resources
Related references
Note: Only part of the references are listed.- Establishment of BmoR-based biosensor to screen isobutanol overproducer
- (2019) Huan Yu et al. Microbial Cell Factories
- CRISPR-Cas9-assisted native end-joining editing offers a simple strategy for efficient genetic engineering in Escherichia coli
- (2019) Chaoyong Huang et al. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
- Simultaneous repression of multiple bacterial genes using nonrepetitive extra-long sgRNA arrays
- (2019) Alexander C. Reis et al. NATURE BIOTECHNOLOGY
- Evolved Cas9 variants with broad PAM compatibility and high DNA specificity
- (2018) Johnny H. Hu et al. NATURE
- Utilization of rare codon-rich markers for screening amino acid overproducers
- (2018) Bo Zheng et al. Nature Communications
- Revisiting the Mechanisms Involved in Calcium Chloride Induced Bacterial Transformation
- (2017) Azka Asif et al. Frontiers in Microbiology
- Analysis of RecA-independent recombination events between short direct repeats related to a genomic island and to a plasmid in Escherichia coli K12
- (2017) María F. Azpiroz et al. PeerJ
- A CRISPR-Cas9 Assisted Non-Homologous End-Joining Strategy for One-step Engineering of Bacterial Genome
- (2016) Tianyuan Su et al. Scientific Reports
- Rich Medium Composition Affects Escherichia coli Survival, Glycation, and Mutation Frequency during Long-Term Batch Culture
- (2015) Karin E. Kram et al. APPLIED AND ENVIRONMENTAL MICROBIOLOGY
- Multigene Editing in the Escherichia coli Genome via the CRISPR-Cas9 System
- (2015) Yu Jiang et al. APPLIED AND ENVIRONMENTAL MICROBIOLOGY
- DECKO: Single-oligo, dual-CRISPR deletion of genomic elements including long non-coding RNAs
- (2015) Estel Aparicio-Prat et al. BMC GENOMICS
- Metabolic engineering of Escherichia coli using CRISPR–Cas9 meditated genome editing
- (2015) Yifan Li et al. METABOLIC ENGINEERING
- Rapid and efficient one-step generation of paired gRNA CRISPR-Cas9 libraries
- (2015) Joana A. Vidigal et al. Nature Communications
- Excision of Unstable Artificial Gene-Specific Inverted Repeats Mediates Scar-Free Gene Deletions in Escherichia coli
- (2014) Crystal Jing Ying Tear et al. APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
- Double Nicking by RNA-Guided CRISPR Cas9 for Enhanced Genome Editing Specificity
- (2013) F. Ann Ran et al. CELL
- RNA-guided editing of bacterial genomes using CRISPR-Cas systems
- (2013) Wenyan Jiang et al. NATURE BIOTECHNOLOGY
- A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity
- (2012) M. Jinek et al. SCIENCE
- Plasmid uptake by bacteria: a comparison of methods and efficiencies
- (2009) Naoto Yoshida et al. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
- Enzymatic assembly of DNA molecules up to several hundred kilobases
- (2009) Daniel G Gibson et al. NATURE METHODS
- Mechanisms of Recombination: Lessons fromE. coli
- (2008) Nicole S. Persky et al. CRITICAL REVIEWS IN BIOCHEMISTRY AND MOLECULAR BIOLOGY
- How does plasmid DNA penetrate cell membranes in artificial transformation process ofEscherichia coli?
- (2008) Subrata Panja et al. MOLECULAR MEMBRANE BIOLOGY
Publish scientific posters with Peeref
Peeref publishes scientific posters from all research disciplines. Our Diamond Open Access policy means free access to content and no publication fees for authors.
Learn MoreBecome a Peeref-certified reviewer
The Peeref Institute provides free reviewer training that teaches the core competencies of the academic peer review process.
Get Started