Efficient gene knockout in primary human and murine myeloid cells by non-viral delivery of CRISPR-Cas9

The Myeloid Cell Compartment—Cell by Cell

(2019) Kevin Bassler et al.   Annual Review of Immunology

CRISPR-Based Tools in Immunity

(2019) Dimitre R. Simeonov et al.   Annual Review of Immunology

Differential Effects of Depleting versus Programming Tumor-Associated Macrophages on Engineered T Cells in Pancreatic Ductal Adenocarcinoma

(2019) Ingunn M. Stromnes et al.   Cancer Immunology Research

Autophagy regulates inflammatory programmed cell death via turnover of RHIM-domain proteins

(2019) Junghyun Lim et al.   eLife

Cas9+ conditionally-immortalized macrophages as a tool for bacterial pathogenesis and beyond

(2019) Allison W Roberts et al.   eLife

CRISPR RNAs trigger innate immune responses in human cells

(2018) Sojung Kim et al.   GENOME RESEARCH

Optimized RNP transfection for highly efficient CRISPR/Cas9-mediated gene knockout in primary T cells

(2018) Akiko Seki et al.   JOURNAL OF EXPERIMENTAL MEDICINE

Myeloid-targeted immunotherapies act in synergy to induce inflammation and antitumor immunity

(2018) Curtis J. Perry et al.   JOURNAL OF EXPERIMENTAL MEDICINE

Distinct progenitor lineages contribute to the heterogeneity of plasmacytoid dendritic cells

(2018) Patrick Fernandes Rodrigues et al.   NATURE IMMUNOLOGY

In vitro–transcribed guide RNAs trigger an innate immune response via the RIG-I pathway

(2018) Beeke Wienert et al.   PLOS BIOLOGY

Strategies to Genetically Modulate Dendritic Cells to Potentiate Anti-Tumor Responses in Hematologic Malignancies

(2018) Annelisa M. Cornel et al.   Frontiers in Immunology

CRISPR/Cas9 Immunoengineering of Hoxb8-Immortalized Progenitor Cells for Revealing CCR7-Mediated Dendritic Cell Signaling and Migration Mechanisms in vivo

(2018) Swantje I. Hammerschmidt et al.   Frontiers in Immunology

CSF1R inhibitors exhibit anti-tumor activity in acute myeloid leukemia by blocking paracrine signals from support cells

(2018) David K. Edwards et al.   BLOOD

Guide Swap enables genome-scale pooled CRISPR–Cas9 screening in human primary cells

(2018) Pamela Y. Ting et al.   NATURE METHODS

Am I ready for CRISPR? A user's guide to genetic screens

(2017) John G. Doench   NATURE REVIEWS GENETICS

Improving CRISPR–Cas specificity with chemical modifications in single-guide RNAs

(2017) Daniel E Ryan et al.   NUCLEIC ACIDS RESEARCH

A Convenient Cas9-based Conditional Knockout Strategy for Simultaneously Targeting Multiple Genes in Mouse

(2017) Jiang Chen et al.   Scientific Reports

Tissue-Resident Macrophage Ontogeny and Homeostasis

(2016) Florent Ginhoux et al.   IMMUNITY

Macrophages in Tissue Repair, Regeneration, and Fibrosis

(2016) Thomas A. Wynn et al.   IMMUNITY

Complement pathway amplifies caspase-11–dependent cell death and endotoxin-induced sepsis severity

(2016) Brooke A. Napier et al.   JOURNAL OF EXPERIMENTAL MEDICINE

Suppression of microRNA activity amplifies IFN-γ-induced macrophage activation and promotes anti-tumour immunity

(2016) Caroline Baer et al.   NATURE CELL BIOLOGY

Highly Efficient Genome Editing of Murine and Human Hematopoietic Progenitor Cells by CRISPR/Cas9

(2016) Michael C. Gundry et al.   Cell Reports

Macrophages: Development and Tissue Specialization

(2015) Chen Varol et al.   Annual Review of Immunology

A Genome-wide CRISPR Screen in Primary Immune Cells to Dissect Regulatory Networks

(2015) Oren Parnas et al.   CELL

Chemically modified guide RNAs enhance CRISPR-Cas genome editing in human primary cells

(2015) Ayal Hendel et al.   NATURE BIOTECHNOLOGY

A stratified myeloid system, the challenge of understanding macrophage diversity

(2015) F. Geissmann et al.   SEMINARS IN IMMUNOLOGY

Dissecting the Tumor Myeloid Compartment Reveals Rare Activating Antigen-Presenting Cells Critical for T Cell Immunity

(2014) Miranda L. Broz et al.   CANCER CELL

CRISPR-Cas9 Knockin Mice for Genome Editing and Cancer Modeling

(2014) Randall J. Platt et al.   CELL

The Dendritic Cell Lineage: Ontogeny and Function of Dendritic Cells and Their Subsets in the Steady State and the Inflamed Setting

(2013) Miriam Merad et al.   Annual Review of Immunology

Increased Numbers of Monocyte-Derived Dendritic Cells during Successful Tumor Immunotherapy with Immune-Activating Agents

(2013) S. Kuhn et al.   JOURNAL OF IMMUNOLOGY

A Human In Vitro Whole Blood Assay to Predict the Systemic Cytokine Response to Therapeutic Oligonucleotides Including siRNA

(2013) Christoph Coch et al.   PLoS One

Efficient transduction of myeloid cells by an HIV-1-derived lentiviral vector that packages the Vpx accessory protein

(2012) S Bobadilla et al.   GENE THERAPY

A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity

(2012) M. Jinek et al.   SCIENCE

Evaluation of transduction efficiency in macrophage colony-stimulating factor differentiated human macrophages using HIV-1 based lentiviral vectors

(2011) Francisco J Leyva et al.   BMC BIOTECHNOLOGY

Optimized Protocol for Efficient Transfection of Dendritic Cells without Cell Maturation

(2011) Robert Bowles et al.   Jove-Journal of Visualized Experiments

Development of Monocytes, Macrophages, and Dendritic Cells

(2010) F. Geissmann et al.   SCIENCE