Article
Food Science & Technology
Xiya Zhang, Mingyue Ding, Chensi Zhang, Yexuan Mao, Youyi Wang, Peipei Li, Haiyang Jiang, Zhanhui Wang, Xuezhi Yu
Summary: The researchers developed a rapid icELISA method for monitoring brevetoxin levels in oysters, providing a useful tool for the detection of these toxins in shellfish samples.
Article
Chemistry, Applied
Shunsuke Fujii, Tomoe Ohta, Riho Ehama, Mizuki Irikida, Shuichi Nomura, Yukihiro Shoyama, Takuhiro Uto
Summary: This study developed a simple and reliable method for the detection and quantification of FMN in food samples, and successfully applied it in cell-based assays.
Article
Chemistry, Applied
Xiaoyang Yin, Huaming Li, Shuangmin Wu, Yanrong Lu, Yalin Yang, Liangni Qin, Long Li, Jiaxu Xiao, Jixiang Liang, Yu Si, Tao Le, Dapeng Peng
Summary: A specific and sensitive monoclonal antibody (McAb) was developed to detect pymetrozine residues using an indirect competitive enzyme-linked immunosorbent assay (icELISA) with a 50% inhibition value (IC50) of 7.70 μg/L. This method showed little cross-reactivity with other pesticides and demonstrated high accuracy in detecting pymetrozine residues in various food samples.
Article
Chemistry, Analytical
Haijiao Gao, Guyue Cheng, Hui Wang, Ting Chen, Chao Xu, Houquan Lv, Heying Zhang, Ren Hou, Yulian Wang, Dapeng Peng, Zhonghui Yuan
Summary: A broad-spectrum monoclonal antibody (mAb)-based indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) was developed for rapidly screening androgens in various animal tissues. The method showed good correlation with HPLC-MS/MS results, demonstrating its effectiveness and accuracy for detecting androgens in animal tissues.
MICROCHEMICAL JOURNAL
(2021)
Article
Biochemical Research Methods
Supaluk Krittanai, Rattanathorn Choonong, Khunkhang Butdapheng, Jakkrit Jabsanthia, Seiichi Sakamoto, Waraporn Putalun
Summary: This study aimed to develop an enzyme-linked immunosorbent assay (ELISA) using a glabridin-specific antibody. The glabridin-bovine serum albumin conjugates were injected into mice to produce hybridomas, and a highly specific antibody against glabridin was produced. The developed ELISA method showed high sensitivity and specificity for quantifying glabridin in plant materials and human serum samples.
PHYTOCHEMICAL ANALYSIS
(2023)
Article
Chemistry, Applied
Long Xu, Fanfan Yang, Alberto C. P. Dias, Xiaoying Zhang
Summary: Sunset yellow is a commonly used food colorant, but excessive and illegal use may pose health risks. Researchers have applied quantum dots in the analysis of sunset yellow and developed two new detection methods. These methods are efficient, highly sensitive, and capable of distinguishing sunset yellow from analogues.
Article
Virology
Fan Yang, Yixin Xiao, Fumin Liu, Hangping Yao, Nanping Wu, Haibo Wu
Summary: A rapid detection method for H7N9 avian influenza virus (AIV) was established using monoclonal antibodies against hemagglutinin (HA) to develop an antigen-capture enzyme-linked immunosorbent assay (AC-ELISA). AC-ELISA showed high specificity and sensitivity, with a detection limit of 3.9 ng/mL for H7N9 HA protein and 2(-2)HA unit/100 mu L for live H7N9 AIV. The technique proved to be rapid and practical for H7N9 AIV detection, with a sensitivity of 94.96% and specificity of 88.24% compared to conventional virus isolation detection.
JOURNAL OF MEDICAL VIROLOGY
(2021)
Article
Chemistry, Applied
Lulu Zhou, Guifen Chen, Min Chen, Xinying Lu, Yijie Xi, Yun Zhi
Summary: An indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) using a highly sensitive and specific monoclonal antibody against avilamycin was developed. The optimized ic-ELISA showed low detection limits and good correlation and reliability compared to HPLC.
FOOD ADDITIVES AND CONTAMINANTS PART A-CHEMISTRY ANALYSIS CONTROL EXPOSURE & RISK ASSESSMENT
(2022)
Article
Chemistry, Analytical
Hui Li, Menglu Li, Yucai Yang, Fei Wang, Feng Wang, Chao Li
Summary: A direct method has been described to establish the correlation between non-nucleic acid analytes and the CRISPR/Cas12a system using rationally designed DNA strands, enabling ultrasensitive detection of biomarkers from different species. The signal output is highly optional and the sensing principle is akin to ELISA, allowing for direct application on the currently available ELISA platform, further facilitating its use in medical diagnostics.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Multidisciplinary
Jian Zhou, Aihong Wu, Liguang Xu, Hua Kuang, Liqiang Liu, Chuanlai Xu, Xinxin Xu
Summary: In this study, two semi-antigens were designed and a highly specific monoclonal antibody against diquat was obtained. The mAb had a half maximal inhibitory concentration of 0.63 ng mL(-1) and a limit of detection of 0.14 ng mL(-1). An immunochromatographic assay was developed using this specific mAb, with a cut-off value of 0.1 mg kg(-1) in fruit samples, confirming its applicability in real samples. Test strips offer advantages over instrumental methods.
NEW JOURNAL OF CHEMISTRY
(2023)
Article
Biochemical Research Methods
Celine Desvignes, David Ternant, Thierry Lecomte, Astrid Lievre, Marc Ohresser, Romain Chautard, William Raoul, Gilles Paintaud
Summary: An ELISA assay measuring ramucirumab serum concentrations was developed and validated, suitable for pharmacokinetic studies of ramucirumab with good sensitivity, accuracy, reproducibility.
Article
Food Science & Technology
Lin Wei, Deyan Xu, Bei Yuan, Chengchen Pang, Haitao Xu, Kunying Nie, Qingqing Yang, Sibel A. Ozkan, Yanyan Zhang, Yemin Guo, Xia Sun
Summary: This study successfully developed a new MBs-icELISA method for detecting AFB(1) with high sensitivity and linear correlation, significantly reducing the detection time compared to conventional ELISA methods.
Article
Chemistry, Applied
Zile Wang, Pimiao Zheng, Jianyi Wang, Shuang He, Zhenhui Ren, Yanfang Zhang, Jincheng Xiong, Haiyang Jiang
Summary: An ic-ELISA method based on a broad-spectrum monoclonal antibody was developed for rapid screening of tropane alkaloids in pig urine, pork, and cereal flour samples. The method showed good sensitivity and reliability, with results correlating well with high performance liquid chromatography-tandem mass spectrometry.
Article
Environmental Sciences
Xiaoqi Li, Xiaofan Liu, Zhenzhen Jia, Tian Wang, Hongyan Zhang
Summary: Using primary hepatocytes isolated from male crucian carp uncontaminated with estrogenic EDCs as a culture model, coupled with ELISA, allows for the detection of estrogenic endocrine-disrupting chemicals in food products.
Article
Virology
Duoduo Liu, Dongmiao Chen, Tingting Zhang, Nan Yu, Ruiwen Ren, Yue Chen, Congrong Wang
Summary: This study developed anti-YFV NS1 monoclonal antibodies using monoclonal antibody technology and established a YFV NS1 antigen-capture ELISA with high specificity and sensitivity.
JOURNAL OF MEDICAL VIROLOGY
(2021)
Article
Medicine, Research & Experimental
Cheng Zhang, Huan Cui, Zhongyi Wang, Shishan Dong, Chunmao Zhang, Jiaming Li, Keyin Meng, Yucheng Sun, Juxiang Liu, Zhendong Guo, Ligong Chen
Summary: This study isolated two human influenza virus strains from severe influenza patients in Hebei, China during the 2018 flu season, and found that the H1N1 strain had stronger pathogenicity and higher airborne transmissibility compared to the other strain belonging to the Victoria lineage. These results may help in monitoring influenza mutations and preventing future outbreaks.
JOURNAL OF INTERNATIONAL MEDICAL RESEARCH
(2021)
Article
Chemistry, Multidisciplinary
Xianjin Wang, Penglei Cui, Chungu Xia, Lipeng Wu
Summary: An unprecedented titanium-catalyzed boration reaction of alkyl (pseudo)halides with borane has been reported. This method can suppress undesired hydrodehalogenation products and tolerate other reducing groups, providing a pathway to synthesize alkyl boronate esters. The mechanism involves a possible radical reaction pathway, and the strategy can also be applied to aryl bromides.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Chemistry, Applied
Teng Zhang, Lei Zhang, Ju Xiang Liu, Jian Ping Wang, Ning Peng Wu
Summary: This study successfully synthesized molecularly imprinted microspheres capable of recognizing amantadine and rimantadine, and developed a highly sensitive and easy-to-use fluorescence detection method for rapid screening of these compounds in chicken muscle samples. The method showed high sensitivity, simple operation, and good recovery rates, making it a promising tool for routine residue screening of amantadine and rimantadine in large quantities of chicken muscle samples.
FOOD ADDITIVES AND CONTAMINANTS PART A-CHEMISTRY ANALYSIS CONTROL EXPOSURE & RISK ASSESSMENT
(2021)
Article
Agriculture, Dairy & Animal Science
Jiacheng Meng, Ning Ma, Hailong Liu, Jing Liu, Juxiang Liu, Jianping Wang, Xin He, Xinghua Zhao
Summary: This study successfully established a FLHS model in laying hens by observing significant changes in various biochemical indicators and liver pathology in the FLHS group. Metabolomics analysis identified multiple liver metabolites associated with FLHS pathogenesis, suggesting a link between liver metabolites and ARA metabolism with the pathophysiology of FLHS.
Article
Immunology
Cheng Zhang, Kangkang Guo, Huan Cui, Ligong Chen, Chunmao Zhang, Xuejing Wang, Jiaming Li, Yingying Fu, Zhongyi Wang, Zhendong Guo, Juxiang Liu, Shishan Dong
Summary: The H7N9 virus isolated from a live poultry market in China has demonstrated transmission through direct contact and aerosol in mammals, with adaptive mutations increasing virulence and aerosol transmissibility. This suggests a potential risk of human-to-human transmission through environmental or airborne routes.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Microbiology
Cheng Zhang, Zhendong Guo, Nan Li, Huan Cui, Keyin Meng, Lina Liu, Li Zhao, Shanshan Zhang, Chengfeng Qin, Juxiang Liu, Yuwei Gao, Chunmao Zhang
Summary: Prior infection with SARS-CoV-2 reduces viral replication in Syrian hamsters but does not provide complete sterilizing immunity. It blocks airborne transmission between previously infected and uninfected hamsters, as well as reduces efficiency of direct contact transmission. However, prior infection has limited impact on transmission to naive hamsters via direct contact in the early stage of infection.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Agriculture, Dairy & Animal Science
Yinchuan Pan, Xu Liu, Jing Liu, Jianping Wang, Juxiang Liu, Yanxia Gao, Ning Ma
Summary: In this study, a chemiluminescence sensor was developed using a molecularly imprinted polymer (MIP) for specific detection of organophosphorus residues in milk samples. The sensor showed high sensitivity and reusability, and can be used as a routine tool for detecting OP residues.
JOURNAL OF DAIRY SCIENCE
(2022)
Article
Chemistry, Organic
Penglei Cui, Sida Li, Xianjin Wang, Ming Li, Chun Wang, Lipeng Wu
Summary: In this study, the catalytic deaminative alkylation of phosphine compounds was achieved using visible-light photoredox catalysis. The use of Eosin Y as the photoredox catalyst and visible light significantly promoted the reaction, leading to the successful synthesis of a series of unsymmetrical tertiary phosphines, including compounds with three different substituents that are otherwise difficult to obtain.
Article
Immunology
Huan Cui, Cheng Zhang, Chunmao Zhang, Zhuming Cai, Ligong Chen, Zhaoliang Chen, Kui Zhao, Sina Qiao, Yingchun Wang, Lijia Meng, Shishan Dong, Juxiang Liu, Zhendong Guo
Summary: Lentinan has been shown to have a high degree of protection against influenza virus in the ICR mouse model, making it a potential new anti-influenza drug. Its mechanisms include inhibiting the inflammatory response and reducing cytokine storms caused by influenza virus infection.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Immunology
Cheng Zhang, Huan Cui, Chunmao Zhang, Zhaoliang Chen, Xinyun Jiang, Jun Liu, Zhonghai Wan, Jiping Li, Juxiang Liu, Yuwei Gao, Ningyi Jin, Zhendong Guo
Summary: This study tested the disinfection effect of a portable ionizer on SARS-CoV-2 and influenza A virus, and found that negative ions can effectively reduce the concentration of particulate matter in the air and disinfect viruses stuck to solid surfaces. Additionally, negative ions can efficiently disinfect aerosolized viruses and provide protection to susceptible animals. The study also demonstrated the safety of negative ion exposure in mice.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Immunology
Cheng Zhang, Huan Cui, Entao Li, Zhendong Guo, Tiecheng Wang, Fang Yan, Lina Liu, Yuanguo Li, Di Chen, Keyin Meng, Nan Li, Chengfeng Qin, Juxiang Liu, Yuwei Gao, Chunmao Zhang
Summary: This study found that the B.1.351 variant can efficiently infect and replicate in mice and rats, with a very low transmission risk in mice but moderate transmission risk in rats through direct contact. The study also emphasizes the need for more attention to the potential transmission of SARS-CoV-2 variants in rats and their potential spillback to humans.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Agriculture, Dairy & Animal Science
Huan Cui, Cheng Zhang, Juxiang Liu, Shishan Dong, Kui Zhao, Ligong Chen, Zhaoliang Chen, Yucheng Sun, Zhendong Guo
Summary: This study compared the bacterial aerosol concentrations and bacterial communities in pig houses at different growth stages in Hebei Province, China. It was found that bacterial concentrations, community richness, and diversity increased with pig age. The presence of pathogenic bacteria in the microbial aerosols of pig houses highlights the importance of implementing measures to prevent air pollution.
Article
Veterinary Sciences
Cheng Zhang, Huan Cui, Wuchao Zhang, Lijia Meng, Ligong Chen, Zhongyi Wang, Kui Zhao, Zhaoliang Chen, Sina Qiao, Juxiang Liu, Zhendong Guo, Shishan Dong
Summary: Pseudorabies (PR) is a serious disease affecting the pig industry in China. Understanding the epidemiology of pseudorabies virus (PRV) is crucial. This study found that PRV variant strains have a high prevalence in Hebei Province, causing significant economic losses to the breeding industry and potentially posing a threat to public health.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Multidisciplinary Sciences
Geng Nan Wang, Yi Peng Li, Si Kun Yuan, Hu Zhang, Juan Ren, Xin Ren, Ju Xiang Liu
Summary: This study investigated the intestinal absorption mechanism of chicoric acid (CA) and its bioavailability improvement using natural chitosan. The results showed that CA had poor absorption, but chitosan increased its permeability and bioavailability. The findings provide a basis for the development and utilization of new formulations of CA.
Article
Agriculture, Dairy & Animal Science
Cheng Zhang, Weihao Li, Ligong Chen, Zhaoliang Chen, Xuejing Wang, Qianqian Xu, Hailong Zhang, Huan Chen, Juxiang Liu
Summary: This study investigates the effect of oregano oil combined with macleaya cordata oral solution on broiler growth performance. The results showed that this combination significantly improves broiler growth, as well as serum indices, immune indices, and intestinal morphology. This study provides basic data and technical support for further research on alternatives to antibiotic growth promoters for broilers.
Article
Chemistry, Applied
M. Graca Dias, Elsa Vasco, Francisco Ravasco, Lufsa Oliveira
Summary: This study estimated the vitamin D intake of "adults" and "elderly" populations in Portugal using the TDS methodology. The results showed that the majority of people had inadequate vitamin D intake, well below the Dietary Reference Values.
Article
Chemistry, Applied
Yanan Wang, Jiachen Shi, Yong-Jiang Xu, Chin-Ping Tan, Yuanfa Liu
Summary: This study investigates the variations in lipid digestion profiles among individuals of different ages using in vitro digestion models. The findings suggest that adults have a more comprehensive lipid digestion compared to infants, and infants tend to release shorter chain length and more saturated free fatty acids during digestion. Additionally, the particle sizes in the stomach of the elderly were consistently larger. This study enhances our understanding of how lipids with different degrees of unsaturation undergo digestion in diverse age groups.
Article
Chemistry, Applied
Hyunjong Yu, Huisu Kim, Pahn-Shick Chang
Summary: Chitosan-pectin emulsion-filled hydrogel (EFH) was developed to enhance the bioaccessibility of lipophilic bioactive compounds through intestinal delivery. The EFH, prepared without crosslinking agents, demonstrated improved mechanical strength and compactness with higher pectin concentration. It retained the emulsion at pH 2.0 and released it at pH 7.4, resulting in enhanced release of free fatty acids and improved bioaccessibility of curcumin.
Article
Chemistry, Applied
Tongze Zhang, Siqi Hong, Jia-Rong Zhang, Pin-He Liu, Siyi Li, Zixian Wen, Jianwei Xiao, Guirong Zhang, Olivier Habimana, Nagendra P. Shah, Zhongquan Sui, Harold Corke
Summary: Lactic acid fermentation significantly affects the morphology and physicochemical properties of proso millet starch, including the formation of surface indentations and small pores, decrease in gelatinization temperatures, and changes in hardness and adhesiveness.
Article
Chemistry, Applied
Liqin Kong, Feng Hong, Peng Luan, Yiping Chen, Yaoze Feng, Ming Zhu
Summary: This study presents a novel impedance biosensor using composite nanomaterials and T1R1 as a signal probe, which can competitively and ultra-sensitively detect umami intensity. The biosensor exhibits exceptional analytical performance and is suitable for food flavor evaluation.
Article
Chemistry, Applied
Kunying Lv, Yixin Yang, Qilong Li, Ran Chen, Liang Deng, Yiwei Zhang, Ning Jiang
Summary: Horse's milk, with its high nutritional value and low allergenic proteins, could be a substitute for cow's milk in infant consumption. A proteomic method was used to identify and compare milk fat globule membrane (MFGM) and whey proteins from different horse breeds. The study found differences in protein composition and functionality, which could support the development of formulas more suitable for human infants.
Article
Chemistry, Applied
Enrique Jacobo Diaz-Montana, Helene Brignot, Ramon Aparicio-Ruiz, Thierry Thomas- Danguin, Maria Teresa Morales
Summary: Sensory perception of virgin olive oil is influenced by phenols and volatiles, which are affected by the composition of the oil and biological factors. This study investigated the effect of saliva and phenols on the release of volatiles, and found that the presence of phenols decreased the release of saturated volatiles.
Article
Chemistry, Applied
Wei Zhou, Rui Zhang, Zhen Cai, Fangfang Wu, Yong Hu, Chao Huang, Kun Hu, Yun Chen
Summary: Environmentally friendly and outstanding pH-responsive cationic starch nanoparticles (CSNP) were prepared from pH-sensitive starch. CSNP exhibited nanosize and regular sphere, highly free-flowing molecular chains, and demonstrated excellent pH responsiveness through multiple emulsion/demulsification transitions.
Article
Chemistry, Applied
Andrea Koo, Vinayak Ghate, Weibiao Zhou
Summary: This study suggests that direct seeding may negatively affect the nutritional quality of crops, causing a decrease in ascorbic acid, vitamin K, and total glucosinolate content.
Article
Chemistry, Applied
Tonghao Du, Yazhou Xu, Xiaoyan Xu, Shijin Xiong, Linli Zhang, Biao Dong, Jinqing Huang, Tao Huang, Muyan Xiao, Tao Xiong, Mingyong Xie
Summary: This study successfully improved the ACE inhibitory activity of black sesame seeds by fermenting them with Lactobacillus Plantarum NCU116 and hydrolyzing them using acid protease. The RF-PSO model was used to predict the ACE inhibitory activity during the hydrolysis process. Eight peptides with ACE inhibitory activity were identified from fermented black sesame seed hydrolysates after separation and screening.
Article
Chemistry, Applied
Yao Liu, Songyi Lin, Kexin Liu, Shan Wang, Qiaozhen Liu, Na Sun
Summary: This study analyzed the structural changes of shrimp proteins during digestion, predicted the immunodominant epitopes, and validated their allergenicity. The results showed that shrimp proteins were degraded into peptides during digestion, but still carried IgE epitopes that trigger allergic reactions.
Article
Chemistry, Applied
Tiantian Fu, Hongwei Cao, Yu Zhang, Xiao Guan
Summary: This study investigates the impact of milling on the active components in rice, with a focus on the stability and bioaccessibility of phenols, VB1, and alpha-GABA during cooking and digestion. The findings show that milling exacerbates the instability of gamma-GABA during cooking and VB1 during digestion, and it affects the bioaccessibility of these active compounds.
Article
Chemistry, Applied
Zhihao Yang, Yanru Hou, Min Zhang, Puxin Hou, Chang Liu, Lu Dou, Xiaoyu Chen, Lihua Zhao, Lin Su, Ye Jin
Summary: This study investigated the molecular mechanism of feeding regimes on lamb flavor by using TMT labeling combined with MS. The results showed that pasture-fed groups had higher levels of amino acids and volatile flavor substances compared to concentrate-fed groups. Additionally, several differentially abundant proteins associated with lamb flavor were identified.
Article
Chemistry, Applied
Zixuan Xie, De Zhang, Junyu Zhu, Qianqian Luo, Jun Liu, Jingtao Zhou, Xiaoyong Wang, Yuqiong Chen, Zhi Yu, Dejiang Ni
Summary: This study investigated the acidification of aroma-enhanced black tea during storage. Analysis of non-volatile substances and organic acids using UPLC-Q-TOF/MS and HPLC revealed a decrease in soluble sugars and amino acids, while an increase in organic acids such as oxalic acid, malic acid, and quinic acid. In vitro experiments further demonstrated that the acidification is a result of the decomposition of sugars and amino acids by heating, as well as the oxidation of aromatic aldehydes. Additionally, the study showed that the taste composition of tea infusion is altered, with reduced amino acids, catechins, soluble sugars, and flavonoids. This research provides a theoretical basis for improving the quality of black tea.
Article
Chemistry, Applied
Lin Wang, Falai Ma, Zihan Li, Yan Zhang
Summary: This study developed time-temperature integrators based on amyloglucosidase@Cu3(PO4)2 nanoflowers for monitoring the freshness of chilled pork. The results showed that the integrators were highly reliable and accurate in predicting the quality of chilled pork.
