Functions and regulation of the Brr2 RNA helicase during splicing

Spliceosomal DEAH-Box ATPases Remodel Pre-mRNA to Activate Alternative Splice Sites

(2016) Daniel R. Semlow et al.   CELL

Interplay of cis- and trans-regulatory mechanisms in the spliceosomal RNA helicase Brr2

(2016) Eva Absmeier et al.   CELL CYCLE

CryoEM structures of two spliceosomal complexes: starter and dessert at the spliceosome feast

(2016) Thi Hoang Duong Nguyen et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

Structure of the frequency‐interacting RNA helicase: a protein interaction hub for the circadian clock

(2016) Karen S Conrad et al.   EMBO JOURNAL

Retinitis Pigmentosa Mutations in Bad Response to Refrigeration 2 (Brr2) Impair ATPase and Helicase Activity

(2016) Sarah Ledoux et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Cryo-EM structure of the yeast U4/U6.U5 tri-snRNP at 3.7 Å resolution

(2016) Thi Hoang Duong Nguyen et al.   NATURE

Cryo-EM structure of the spliceosome immediately after branching

(2016) Wojciech P. Galej et al.   NATURE

Substrate-assisted mechanism of RNP disruption by the spliceosomal Brr2 RNA helicase

(2016) Matthias Theuser et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Prp8 retinitis pigmentosa mutants cause defects in the transition between the catalytic steps of splicing

(2016) Megan Mayerle et al.   RNA

Molecular architecture of the human U4/U6.U5 tri-snRNP

(2016) D. E. Agafonov et al.   SCIENCE

The 3.8 A structure of the U4/U6.U5 tri-snRNP: Insights into spliceosome assembly and catalysis

(2016) R. Wan et al.   SCIENCE

Structure of a yeast catalytic step I spliceosome at 3.4 A resolution

(2016) R. Wan et al.   SCIENCE

Molecular architecture of the Saccharomyces cerevisiae activated spliceosome

(2016) R. Rauhut et al.   SCIENCE

Structure of a yeast activated spliceosome at 3.5 A resolution

(2016) C. Yan et al.   SCIENCE

A spliceosome intermediate with loosely associated tri-snRNP accumulates in the absence of Prp28 ATPase activity

(2016) Carsten Boesler et al.   Nature Communications

Single molecule analysis reveals reversible and irreversible steps during spliceosome activation

(2016) Aaron A Hoskins et al.   eLife

The target of the DEAH-box NTP triphosphatase Prp43 in Saccharomyces cerevisiae spliceosomes is the U2 snRNP-intron interaction

(2016) Jean-Baptiste Fourmann et al.   eLife

Structural and functional analysis of the RNA helicase Prp43 from the thermophilic eukaryoteChaetomium thermophilum

(2016) Marcel J. Tauchert et al.   Acta Crystallographica Section F-Structural Biology Communications

SnapShot: Spliceosome Dynamics I

(2015) Markus C. Wahl et al.   CELL

The architecture of the spliceosomal U4/U6.U5 tri-snRNP

(2015) Thi Hoang Duong Nguyen et al.   NATURE

Brr2 plays a role in spliceosomal activation in addition to U4/U6 unwinding

(2015) Lingdi Zhang et al.   NUCLEIC ACIDS RESEARCH

Structural basis of pre-mRNA splicing

(2015) Jing Hang et al.   SCIENCE

Structure of a yeast spliceosome at 3.6-angstrom resolution

(2015) Chuangye Yan et al.   SCIENCE

A composite double-/single-stranded RNA-binding region in protein Prp3 supports tri-snRNP stability and splicing

(2015) Sunbin Liu et al.   eLife

A noncanonical PWI domain in the N-terminal helicase-associated region of the spliceosomal Brr2 protein

(2015) Eva Absmeier et al.   ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY

Brr2p carboxy-terminal Sec63 domain modulates Prp16 splicing RNA helicase

(2014) Olivier Cordin et al.   NUCLEIC ACIDS RESEARCH

The Mtr4 ratchet helix and arch domain both function to promote RNA unwinding

(2014) Lacy L. Taylor et al.   NUCLEIC ACIDS RESEARCH

Novel regulatory principles of the spliceosomal Brr2 RNA helicase and links to retinal disease in humans

(2014) Sina Mozaffari-Jovin et al.   RNA Biology

The DExD/H-box ATPase Prp2p destabilizes and proofreads the catalytic RNA core of the spliceosome

(2014) A. M. Wlodaver et al.   RNA

Structural basis for dual roles of Aar2p in U5 snRNP assembly

(2013) G. Weber et al.   GENES & DEVELOPMENT

Dissection of the factor requirements for spliceosome disassembly and the elucidation of its dissociation products using a purified splicing system

(2013) J.-B. Fourmann et al.   GENES & DEVELOPMENT

Sad1 Counteracts Brr2-Mediated Dissociation of U4/U6.U5 in Tri-snRNP Homeostasis

(2013) Y.-H. Huang et al.   MOLECULAR AND CELLULAR BIOLOGY

RNA catalyses nuclear pre-mRNA splicing

(2013) Sebastian M. Fica et al.   NATURE

Crystal structure of Prp8 reveals active site cavity of the spliceosome

(2013) Wojciech P. Galej et al.   NATURE

Splicing proofreading at 5′ splice sites by ATPase Prp28p

(2013) Fei Yang et al.   NUCLEIC ACIDS RESEARCH

An unanticipated early function of DEAD-box ATPase Prp28 during commitment to splicing is modulated by U5 snRNP protein Prp8

(2013) A. M. Price et al.   RNA

Inhibition of RNA Helicase Brr2 by the C-Terminal Tail of the Spliceosomal Protein Prp8

(2013) S. Mozaffari-Jovin et al.   SCIENCE

Structural Basis of Brr2-Prp8 Interactions and Implications for U5 snRNP Biogenesis and the Spliceosome Active Site

(2013) Thi Hoang Duong Nguyen et al.   STRUCTURE

Crystal Structure of Prp5p Reveals Interdomain Interactions that Impact Spliceosome Assembly

(2013) Zhi-Min Zhang et al.   Cell Reports

Structural analysis of the C-terminal domain of the spliceosomal helicase Prp22

(2012) Denis Kudlinzki et al.   BIOLOGICAL CHEMISTRY

Structure, function and regulation of spliceosomal RNA helicases

(2012) Olivier Cordin et al.   CURRENT OPINION IN CELL BIOLOGY

Brr2p-mediated conformational rearrangements in the spliceosome during activation and substrate repositioning

(2012) D. Hahn et al.   GENES & DEVELOPMENT

The Prp8 RNase H-like domain inhibits Brr2-mediated U4/U6 snRNA unwinding by blocking Brr2 loading onto the U4 snRNA

(2012) S. Mozaffari-Jovin et al.   GENES & DEVELOPMENT

Link of NTR-Mediated Spliceosome Disassembly with DEAH-Box ATPases Prp2, Prp16, and Prp22

(2012) Hsin-Chou Chen et al.   MOLECULAR AND CELLULAR BIOLOGY

Structural bioinformatics of the human spliceosomal proteome

(2012) Iga Korneta et al.   NUCLEIC ACIDS RESEARCH

Structural basis for functional cooperation between tandem helicase cassettes in Brr2-mediated remodeling of the spliceosome

(2012) K. F. Santos et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

RNA helicases in splicing

(2012) Olivier Cordin et al.   RNA Biology

Structure of the yeast U2/U6 snRNA complex

(2012) J. E. Burke et al.   RNA

Prp2-mediated protein rearrangements at the catalytic core of the spliceosome as revealed by dcFCCS

(2012) T. Ohrt et al.   RNA

Staying on message: ensuring fidelity in pre-mRNA splicing

(2012) Daniel R. Semlow et al.   TRENDS IN BIOCHEMICAL SCIENCES

Mechanism for Aar2p function as a U5 snRNP assembly factor

(2011) G. Weber et al.   GENES & DEVELOPMENT

Next generation sequencing of pooled samples reveals new SNRNP200 mutations associated with retinitis pigmentosa

(2011) Paola Benaglio et al.   HUMAN MUTATION

Semiquantitative Proteomic Analysis of the Human Spliceosome via a Novel Two-Dimensional Gel Electrophoresis Method

(2011) D. E. Agafonov et al.   MOLECULAR AND CELLULAR BIOLOGY

DNA Unwinding by ASCC3 Helicase Is Coupled to ALKBH3-Dependent DNA Alkylation Repair and Cancer Cell Proliferation

(2011) Sebastian Dango et al.   MOLECULAR CELL

The crystal structure of Mtr4 reveals a novel arch domain required for rRNA processing

(2010) Ryan N Jackson et al.   EMBO JOURNAL

Prp43p contains a processive helicase structural architecture with a specific regulatory domain

(2010) Hélène Walbott et al.   EMBO JOURNAL

Structural basis for the function of DEAH helicases

(2010) Yangzi He et al.   EMBO REPORTS

The Prp19 complex and the Usp4Sart3 deubiquitinating enzyme control reversible ubiquitination at the spliceosome

(2010) E. J. Song et al.   GENES & DEVELOPMENT

Splicing Factor Cwc22 Is Required for the Function of Prp2 and for the Spliceosome To Escape from a Futile Pathway

(2010) T.-C. Yeh et al.   MOLECULAR AND CELLULAR BIOLOGY

The DEAH Box ATPases Prp16 and Prp43 Cooperate to Proofread 5′ Splice Site Cleavage during Pre-mRNA Splicing

(2010) Prakash Koodathingal et al.   MOLECULAR CELL

Spliceosome discards intermediates via the DEAH box ATPase Prp43p

(2010) R. M. Mayas et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Structural analysis reveals the characteristic features of Mtr4, a DExH helicase involved in nuclear RNA processing and surveillance

(2010) J. R. Weir et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

DEAH-box ATPase Prp16 has dual roles in remodeling of the spliceosome in catalytic steps

(2010) C.-K. Tseng et al.   RNA

Release of SF3 from the intron branchpoint activates the first step of pre-mRNA splicing

(2010) R. M. Lardelli et al.   RNA

Spliceosome Structure and Function

(2010) C. L. Will et al.   Cold Spring Harbor Perspectives in Biology

Autosomal-Dominant Retinitis Pigmentosa Caused by a Mutation in SNRNP200, a Gene Required for Unwinding of U4/U6 snRNAs

(2009) Chen Zhao et al.   AMERICAN JOURNAL OF HUMAN GENETICS

The Spliceosome: Design Principles of a Dynamic RNP Machine

(2009) Markus C. Wahl et al.   CELL

The DEXD/H-box RNA Helicase DDX19 Is Regulated by an α-Helical Switch

(2009) Ruairi Collins et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Common Design Principles in the Spliceosomal RNA Helicase Brr2 and in the Hel308 DNA Helicase

(2009) Vladimir Pena et al.   MOLECULAR CELL

The Evolutionarily Conserved Core Design of the Catalytic Activation Step of the Yeast Spliceosome

(2009) Patrizia Fabrizio et al.   MOLECULAR CELL

Structural evidence for consecutive Hel308-like modules in the spliceosomal ATPase Brr2

(2009) Lingdi Zhang et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Reconstitution of both steps of Saccharomyces cerevisiae splicing with purified spliceosomal components

(2009) Zbigniew Warkocki et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Evidence that U2/U6 helix I promotes both catalytic steps of pre-mRNA splicing and rearranges in between these steps

(2009) M. A. Mefford et al.   RNA

Structure and function of an RNase H domain at the heart of the spliceosome

(2008) Vladimir Pena et al.   EMBO JOURNAL

Structural elucidation of a PRP8 core domain from the heart of the spliceosome

(2008) Dustin B Ritchie et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

ATP-dependent unwinding of U4/U6 snRNAs by the Brr2 helicase requires the C terminus of Prp8

(2008) Corina Maeder et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Crystal structure of the  -finger domain of Prp8 reveals analogy to ribosomal proteins

(2008) K. Yang et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA