Efficient dual-negative selection for bacterial genome editing

Quantitative contribution of efflux to multi-drug resistance of clinical Escherichia coli and Pseudomonas aeruginosa strains

(2019) Olivier Cunrath et al.   EBioMedicine

Editing the microbiome the CRISPR way

(2019) Gayetri Ramachandran et al.   PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES

An update of the suicide plasmid‐mediated genome editing system in Corynebacterium glutamicum

(2019) Ting Wang et al.   Microbial Biotechnology

The CRISPR tool kit for genome editing and beyond

(2018) Mazhar Adli   Nature Communications

Efficient counterselection for Methylococcus capsulatus (Bath) using a mutated pheS gene

(2018) Masahito Ishikawa et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Bacterial genome sequencing in clinical microbiology: a pathogen-oriented review

(2017) F. Tagini et al.   EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES

A bacterial toxin-antitoxin module is the origin of inter-bacterial and inter-kingdom effectors of Bartonella

(2017) Alexander Harms et al.   PLoS Genetics

A Novel and Efficient Method for Bacteria Genome Editing Employing both CRISPR/Cas9 and an Antibiotic Resistance Cassette

(2017) Hong Zhang et al.   Frontiers in Microbiology

Scarless deletion of up to seven methyl-accepting chemotaxis genes with an optimized method highlights key function of CheM in Salmonella Typhimurium

(2017) Stefanie Hoffmann et al.   PLoS One

Coupling the CRISPR/Cas9 System with Lambda Red Recombineering Enables Simplified Chromosomal Gene Replacement in Escherichia coli

(2015) Michael E. Pyne et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

I-SceI-mediated scarless gene modification via allelic exchange in Clostridium

(2015) Ning Zhang et al.   JOURNAL OF MICROBIOLOGICAL METHODS

Precision-engineering the Pseudomonas aeruginosa genome with two-step allelic exchange

(2015) Laura R Hmelo et al.   Nature Protocols

Development of a markerless gene replacement system in Corynebacterium glutamicum using upp as a counter-selection marker

(2014) Wenwen Ma et al.   BIOTECHNOLOGY LETTERS

A versatile and highly efficient method for scarless genome editing in Escherichia coli and Salmonella enterica

(2014) Juhan Kim et al.   BMC BIOTECHNOLOGY

RNA-guided editing of bacterial genomes using CRISPR-Cas systems

(2013) Wenyan Jiang et al.   NATURE BIOTECHNOLOGY

Positive and negative selection using the tetA-sacB cassette: recombineering and P1 transduction in Escherichia coli

(2013) Xin-tian Li et al.   NUCLEIC ACIDS RESEARCH

Multiplex Genome Engineering Using CRISPR/Cas Systems

(2013) L. Cong et al.   SCIENCE

FRUIT, a Scar-Free System for Targeted Chromosomal Mutagenesis, Epitope Tagging, and Promoter Replacement in Escherichia coli and Salmonella enterica

(2012) Anne M. Stringer et al.   PLoS One

Rapid and Highly Efficient Method for Scarless Mutagenesis within the Salmonella enterica Chromosome

(2011) Kathrin Blank et al.   PLoS One

Enzymatic assembly of DNA molecules up to several hundred kilobases

(2009) Daniel G Gibson et al.   NATURE METHODS

The PhoQ histidine kinases ofSalmonellaandPseudomonasspp. are structurally and functionally different: evidence that pH and antimicrobial peptide sensing contribute to mammalian pathogenesis

(2008) Lynne R. Prost et al.   MOLECULAR MICROBIOLOGY