Review
Microbiology
Muhammad Fakhri Zainuddin, Chong Kar Fai, Arbakariya B. Ariff, Leonardo Rios-Solis, Murni Halim
Summary: The production of lipids from oleaginous yeasts involves multiple stages including cultivation, lipid accumulation, biomass harvesting, and lipid extraction. The complex and resistant cell wall of yeasts requires a pretreatment or cell disruption method before solvent extraction. Each recovery method adopts different mechanisms, requiring systematic evaluation before selecting a particular method.
Review
Biotechnology & Applied Microbiology
Mohsen Danaeifar
Summary: Cell lysis is a crucial step in biology and medicine research, with various types of methods available. Traditional approaches and new techniques are being developed to provide more precise and efficient tools while reducing the costs. However, challenges and improvements still remain.
BIOTECHNOLOGY AND BIOENGINEERING
(2022)
Article
Agricultural Engineering
Swati Das, Sovik Das, M. M. Ghangrekar
Summary: This study demonstrates a cost-effective approach for enhanced lipid recovery in algal biodiesel production by using solvent-free cell lysis with fungal enzyme. By fabricating a triple chamber photosynthetic microbial fuel cell (PMFC), the negatively charged lipid ions were successfully extracted from the cathodic chamber. In addition, enzyme-assisted de-oiled algal biomass was used for bioelectricity production and wastewater treatment. Therefore, this investigation provides a promising solution for sustainable biofuel production and environmental remediation.
BIORESOURCE TECHNOLOGY
(2022)
Article
Biochemical Research Methods
Austin L. Culberson, Mason A. Chilmonczyk, Peter A. Kottke, Annie C. Bowles-Welch, Delta Ghoshal, Andrei G. Fedorov
Summary: Real-time diagnostics of the biochemical state within cells remains challenging, but an integrated microfluidic platform allows for rapid and high-throughput analysis of intracellular state to discover critical quality attributes.
Article
Immunology
Lei Zhang, Ting Chen, Ye Wang, Shengwei Zhang, Qingyu Lv, Decong Kong, Hua Jiang, Yuling Zheng, Yuhao Ren, Wenhua Huang, Peng Liu, Yongqiang Jiang
Summary: Metagenomic next-generation sequencing (mNGS) is a novel strategy increasingly used in clinic for pathogen detection. In this study, three DNA extraction methods were compared, and the enzymatic-based method was found to have excellent performance in long-read mNGS-based pathogen identification and potential diseases diagnosis.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Gautam Kumar, Kritika Engle
Summary: Covering: 2015 to 2022, this study summarizes recent advances in understanding the activity of natural drugs or agents that directly inhibit bacterial membranes, membrane components, and membrane biosynthetic enzymes. These natural products serve as a starting point for drug discovery and development, which sometimes require modification to meet drug criteria. Microorganisms and plant metabolites have also contributed to antibiotics for noninfectious diseases.
NATURAL PRODUCT REPORTS
(2023)
Review
Biochemistry & Molecular Biology
Md Mijanur Rahman, Nushin Hosano, Hamid Hosano
Summary: Microalgae have great potential as a sustainable source for various compounds, and understanding the cell disruption and extraction techniques is crucial for improving efficiency and exploring new methods. This review provides an overview of microalgal cell disruption techniques, analyzes their performance and challenges, and discusses the potential of pulsed electric field-assisted treatments as an effective option for microalgae compound extraction.
Editorial Material
Biochemistry & Molecular Biology
Yupeng Wang, Feng Shao
Summary: The study identified NINJ1 as a mediator of plasma membrane rupture following programmed cell death, challenging the previous notion that this event was passive.
Review
Chemistry, Analytical
Emil Grigorov, Boris Kirov, Marin B. Marinov, Vassil Galabov
Summary: Cell lysis is a crucial process to extract important information about DNA or RNA of an organism. This article provides a comprehensive review of various methods for effective cellular disintegration, categorized into six types. The advantages and disadvantages of each method are outlined, with a concise table comparing benefits based on criteria like cost, efficiency, and difficulty.
Article
Management
Varun Gupta, Dmitry Ivanov, Tsan-Ming Choi
Summary: This study examines the impact of supply disruption timing on pricing decisions, finding that order quantity is influenced by price leadership, and higher levels of disruption lead to higher market retail prices in the Nash game. The timing of orders plays a critical role in pricing strategy.
OMEGA-INTERNATIONAL JOURNAL OF MANAGEMENT SCIENCE
(2021)
Article
Chemistry, Analytical
Mian Yang, Jorvani Cruz Villarreal, Nethmi Ariyasinghe, Rory Kruithoff, Robert Ros, Alexandra Ros
Summary: Advancements in single-cell analysis technologies are crucial for characterizing cells, studying cell biology, drug design, and cancer diagnosis and treatment. Current approaches using fluorescent antibody-binding technology are limited by high background and cross-talk from fluorescent labels, highlighting the need for novel label-free methods.
ANALYTICAL CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Takanobu Takata, Akiko Sakasai-Sakai, Masayoshi Takeuchi
Summary: Cardiovascular disease is a lifestyle-related disease caused by dysfunction and cell death of cardiomyocytes. Cardiac fibroblasts play a crucial role in protecting and repairing cardiomyocytes. Toxic AGEs are associated with cardiovascular diseases and induce cell death in cardiomyocytes.
Article
Chemistry, Analytical
Suhyeon Kim, Hyoryung Nam, Eunji Lee, Ji-Won Huh, You-Me Kim, Hyungkook Jeon, Geunbae Lim
Summary: We have developed a multifunctional microfluidic device that can lyse cells and separate intracellular components, offering advantages of simple fabrication, low cost, and bubble-free operation. The device utilizes an amplified electric field in the ion-depletion region formed by ion concentration polarization, allowing for sequential processing of cell lysis and separation of nano-sized components.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Editorial Material
Chemistry, Multidisciplinary
Kristie Darrah, Alexander Deiters
Summary: The light-induced lysis of red blood cell carriers offers a delivery strategy for protein therapeutics with precise spatiotemporal control.
ACS CENTRAL SCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Mie Riisom, Stephen M. F. Jamieson, Christian G. Hartinger
Summary: This study aimed to investigate different protocols for efficient and reproducible lysis of cancer cells and found that chemical lysis with the RIPA buffer was the most efficient method. Minimal differences were found for experiments measuring the overall cell uptake of the Ru complex.
Article
Biotechnology & Applied Microbiology
Cristina Maria Zanette, Andre Bellin Mariano, Yuri Sussumu Yukawa, Israel Mendes, Michele Rigon Spier
JOURNAL OF APPLIED PHYCOLOGY
(2019)
Article
Engineering, Chemical
Cristina Angelica T. Toro, Joao Luiz A. Dagostin, Erika de Castro Vasques, Michele R. Spier, Luciana Igarashi-Mafra, Tirzha L. P. Dantas
CANADIAN JOURNAL OF CHEMICAL ENGINEERING
(2020)
Article
Biotechnology & Applied Microbiology
Vinicius Jose Bolognesi, Michele Rigon Spier, Carlos Eduardo Rocha Garcia
FOOD TECHNOLOGY AND BIOTECHNOLOGY
(2020)
Article
Chemistry, Applied
Jamille S. Correa, Julia O. Primo, Carla Bittencourt, Dienifer F. L. Horsth, Eduardo Radovanovic, Alceu T. Silveira-Jr, Henrique E. Toma, Cristina M. Zanette, Fauze J. Anaissi
Summary: A Zn-abietate complex synthesized from resin obtained from Pinus Elliottii in the Southern United States exhibits excellent antibacterial properties, making it suitable for applications in paints.
Article
Food Science & Technology
Bianca Peron-Schlosser, Danielle Carpine, Regina Maria Matos Jorge, Michele Rigon Spier
Summary: This study demonstrated the feasibility of producing sustainable and biodegradable packaging films using the byproduct of wheat milling, glue flour (GF). The properties of the films were mainly influenced by the type of plasticizer and concentration of GF. The casting technique used in the study provides valuable insights for potential scale-up production.
JOURNAL OF FOOD SCIENCE
(2021)
Article
Chemistry, Multidisciplinary
Julia de O. Primo, Dienifer F. Horsth, Jamille de S. Correa, Arkaprava Das, Carla Bittencourt, Polona Umek, Ana Guilherme Buzanich, Martin Radtke, Kirill Yusenko, Cristina Zanette, Fauze J. Anaissi
Summary: This study synthesized Ag/ZnO nanoparticles using two green synthesis routes, with cassava starch as a fuel and Aloe vera as a reducing and stabilizing agent. The nanoparticles showed high efficiency in disinfecting lake water contaminated with bacteria, especially Escherichia coli (E. coli). The results indicate that Ag/ZnO nanoparticles are a promising candidate for treating bacterially contaminated wastewaters due to their ease of preparation, low cost, and disinfection efficiency.
Article
Chemistry, Multidisciplinary
Dienifer F. L. Horsth, Julia de O. Primo, Nayara Balaba, Jamille S. Correa, Cristina M. Zanette, Douglas K. Silva, Carla Bittencourt, Fauze J. Anaissi
Summary: This study reports the synthesis of boehmite aluminum oxide hydroxide particles from the recycling of metallic can seals, with the addition of silver nanoparticles reduced by Aloe Vera extract, showing high efficiency in water treatment. The ability to reuse the sample multiple times demonstrates the potential for sustainable circular economy practices.
Article
Engineering, Chemical
Luis Alberto Gallo-Garcia, Bianca Peron-Schlosser, Danielle Carpine, Rodolfo Mesquita de Oliveira, Bruno Matheus Simoes, Adriana Passos Dias, Fabio Yamashita, Michele Rigon Spier
Summary: This research developed blends of corn starch and PBAT films with the addition of CP biomass. The film with 5.0% CP biomass content showed the best tensile strength, elongation, and water vapor permeability. Ultrasound treatment did not improve the properties of the films. However, the CP biomass in the films provided natural bio-pigmentation's protection against UV light.
JOURNAL OF FOOD PROCESS ENGINEERING
(2022)
Article
Biochemistry & Molecular Biology
Nayara Balaba, Silvia Jaerger, Dienifer F. L. Horsth, Julia de O. Primo, Jamille de S. Correa, Carla Bittencourt, Cristina M. Zanette, Fauze J. Anaissi
Summary: This study reported the synthesis of structured MgO using feedstock starch, citrus pectin, and Aloe vera leaf, which are abundant, low-cost, and non-toxic green fuels. The formed oxides exhibited high porosity and were evaluated for their antimicrobial activity. Characterization techniques such as EDXRF, XRD, FTIR, and SEM were used to analyze the samples. The results showed that the sample morphology depended on the organic fuel used during the synthesis. The MgO-St, MgO-CP, and MgO-Av oxides showed effective antimicrobial activity against Staphylococcus aureus and Escherichia coli, as well as antifungal activity against Candida albicans.
Article
Chemistry, Multidisciplinary
Jamille de S. Correa, Julia de O. Primo, Nayara Balaba, Christoph Pratsch, Stephan Werner, Henrique E. Toma, Fauze J. Anaissi, Ruddy Wattiez, Cristina M. Zanette, Rob C. A. Onderwater, Carla Bittencourt
Summary: Co-abietate and Cu-abietate complexes were synthesized using a low-cost and eco-friendly method by combining Pinus elliottii resin with an aqueous solution of CuSO4/CoSO4 at a mild temperature. The obtained complexes are functional pigments with antipathogenic activity and can be used in commercial architectural paints. The pigments were characterized using various analytical techniques, and their antibacterial and antiviral properties were evaluated. The Co-abietate and Cu-abietate complexes showed significant inactivation against SARS-CoV-2 and exhibited strong antibacterial activity against S. aureus and E. coli. These findings suggest that the complexes can be applied as pigments in architectural paints for healthcare centers, homes, and public places.
Article
Agriculture, Multidisciplinary
Francieli Begnini Siepmann, Beatriz Sousa de Almeida, Tatiane Aparecida Gomes, Nina Waszczynskyj, Michele Rigon Spier
Summary: This study investigates the interplay between biochemical characteristics and the generation of volatile compounds in eleven type II sourdough fermented by single strains of lactic acid bacteria (LAB). The results show that the addition of LAB and an increase in acetic acid content can influence the characteristics of the dough, while different LAB strains can affect the consumption of carbohydrates by autochthonous yeast, thus influencing the volatile compounds in the dough.
SEMINA-CIENCIAS AGRARIAS
(2022)