Cas3/I-C mediated target DNA recognition and cleavage during CRISPR interference are independent of the composition and architecture of Cascade surveillance complex

Fidelity of prespacer capture and processing is governed by the PAM mediated interactions of Cas1-2 adaptation complex in CRISPR-Cas type I-E system

(2019) Kakimani Nagarajan Yoganand et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

The Biology of CRISPR-Cas: Backward and Forward

(2018) Frank Hille et al.   CELL

Repetitive DNA Reeling by the Cascade-Cas3 Complex in Nucleotide Unwinding Steps

(2018) Luuk Loeff et al.   MOLECULAR CELL

Structure basis for RNA-guided DNA degradation by Cascade and Cas3

(2018) Yibei Xiao et al.   SCIENCE

Determining the Specificity of Cascade Binding, Interference, and Primed Adaptation In Vivo in the Escherichia coli Type I-E CRISPR-Cas System

(2018) Lauren A. Cooper et al.   mBio

Assembly and Translocation of a CRISPR-Cas Primed Acquisition Complex

(2018) Kaylee E. Dillard et al.   CELL

CRISPR RNA-guided DNA cleavage by reconstituted Type I-A immune effector complexes

(2018) Sonali Majumdar et al.   EXTREMOPHILES

Structural Variation of Type I-F CRISPR RNA Guided DNA Surveillance

(2017) Patrick Pausch et al.   MOLECULAR CELL

Target DNA recognition and cleavage by a reconstituted Type I-G CRISPR-Cas immune effector complex

(2016) Sonali Majumdar et al.   EXTREMOPHILES

DNA Targeting by a Minimal CRISPR RNA-Guided Cascade

(2016) Megan L. Hochstrasser et al.   MOLECULAR CELL

CRISPR Immunological Memory Requires a Host Factor for Specificity

(2016) James K. Nuñez et al.   MOLECULAR CELL

Structural basis for promiscuous PAM recognition in type I–E Cascade from E. coli

(2016) Robert P. Hayes et al.   NATURE

Asymmetric positioning of Cas1–2 complex and Integration Host Factor induced DNA bending guide the unidirectional homing of protospacer in CRISPR-Cas type I-E system

(2016) K.N.R. Yoganand et al.   NUCLEIC ACIDS RESEARCH

Diverse evolutionary roots and mechanistic variations of the CRISPR-Cas systems

(2016) Prarthana Mohanraju et al.   SCIENCE

Surveillance and Processing of Foreign DNA by the Escherichia coli CRISPR-Cas System

(2015) Sy Redding et al.   CELL

Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System

(2015) Bernd Zetsche et al.   CELL

Two Distinct DNA Binding Modes Guide Dual Roles of a CRISPR-Cas Protein Complex

(2015) Timothy R. Blosser et al.   MOLECULAR CELL

An updated evolutionary classification of CRISPR–Cas systems

(2015) Kira S. Makarova et al.   NATURE REVIEWS MICROBIOLOGY

Interference activity of a minimal Type I CRISPR–Cas system fromShewanella putrefaciens

(2015) Srivatsa Dwarakanath et al.   NUCLEIC ACIDS RESEARCH

Directional R-Loop Formation by the CRISPR-Cas Surveillance Complex Cascade Provides Efficient Off-Target Site Rejection

(2015) Marius Rutkauskas et al.   Cell Reports

Structures of CRISPR Cas3 offer mechanistic insights into Cascade-activated DNA unwinding and degradation

(2014) Yanwu Huo et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Cas1–Cas2 complex formation mediates spacer acquisition during CRISPR–Cas adaptive immunity

(2014) James K Nuñez et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

In vitro assembly and activity of an archaeal CRISPR-Cas type I-A Cascade interference complex

(2014) André Plagens et al.   NUCLEIC ACIDS RESEARCH

Molecular insights into DNA interference by CRISPR-associated nuclease-helicase Cas3

(2014) Bei Gong et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Direct observation of R-loop formation by single RNA-guided Cas9 and Cascade effector complexes

(2014) Mark D. Szczelkun et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

CasA mediates Cas3-catalyzed target degradation during CRISPR RNA-guided interference

(2014) M. L. Hochstrasser et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

In vitro reconstitution of Cascade-mediated CRISPR immunity in Streptococcus thermophilus

(2013) Tomas Sinkunas et al.   EMBO JOURNAL

In VitroReconstitution of anEscherichia coliRNA-guided Immune System Reveals Unidirectional, ATP-dependent Degradation of DNA Target

(2013) Sabin Mulepati et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Active site plasticity enables metal-dependent tuning of Cas5d nuclease activity in CRISPR-Cas type I-C system

(2013) Ankita Punetha et al.   NUCLEIC ACIDS RESEARCH

Genetic determinants of PAM-dependent DNA targeting and pre-crRNA processing inSulfolobus islandicus

(2013) Wenfang Peng et al.   RNA Biology

One-Step Cloning and Chromosomal Integration of DNA

(2013) François St-Pierre et al.   ACS Synthetic Biology

Type I-E CRISPR-Cas Systems Discriminate Target from Non-Target DNA through Base Pairing-Independent PAM Recognition

(2013) Edze R. Westra et al.   PLoS Genetics

Characterization of the CRISPR/Cas Subtype I-A System of the Hyperthermophilic Crenarchaeon Thermoproteus tenax

(2012) A. Plagens et al.   JOURNAL OF BACTERIOLOGY

Structure and Mechanism of the CMR Complex for CRISPR-Mediated Antiviral Immunity

(2012) Jing Zhang et al.   MOLECULAR CELL

CRISPR Immunity Relies on the Consecutive Binding and Degradation of Negatively Supercoiled Invader DNA by Cascade and Cas3

(2012) Edze R. Westra et al.   MOLECULAR CELL

Proteins and DNA elements essential for the CRISPR adaptation process in Escherichia coli

(2012) Ido Yosef et al.   NUCLEIC ACIDS RESEARCH

In Vivo Protein Interactions and Complex Formation in the Pectobacterium atrosepticum Subtype I-F CRISPR/Cas System

(2012) Corinna Richter et al.   PLoS One

Cas9-crRNA ribonucleoprotein complex mediates specific DNA cleavage for adaptive immunity in bacteria

(2012) G. Gasiunas et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Cascade-mediated binding and bending of negatively supercoiled DNA

(2012) Edze R. Westra et al.   RNA Biology

Cas5d processes pre-crRNA and is a member of a larger family of CRISPR RNA endonucleases

(2012) E. L. Garside et al.   RNA

A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity

(2012) M. Jinek et al.   SCIENCE

Cas5d Protein Processes Pre-crRNA and Assembles into a Cascade-like Interference Complex in Subtype I-C/Dvulg CRISPR-Cas System

(2012) Ki Hyun Nam et al.   STRUCTURE

Molecular memory of prior infections activates the CRISPR/Cas adaptive bacterial immunity system

(2012) Kirill A. Datsenko et al.   Nature Communications

Helicase dissociation and annealing of RNA-DNA hybrids byEscherichia coliCas3 protein

(2011) Jamieson A. L. Howard et al.   BIOCHEMICAL JOURNAL

Structure and activity of the Cas3 HD nuclease MJ0384, an effector enzyme of the CRISPR interference

(2011) Natalia Beloglazova et al.   EMBO JOURNAL

Cas3 is a single-stranded DNA nuclease and ATP-dependent helicase in the CRISPR/Cas immune system

(2011) Tomas Sinkunas et al.   EMBO JOURNAL

Structural and Biochemical Analysis of Nuclease Domain of Clustered Regularly Interspaced Short Palindromic Repeat (CRISPR)-associated Protein 3 (Cas3)

(2011) Sabin Mulepati et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Structures of the RNA-guided surveillance complex from a bacterial immune system

(2011) Blake Wiedenheft et al.   NATURE

Structural basis for CRISPR RNA-guided DNA recognition by Cascade

(2011) Matthijs M Jore et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

The Streptococcus thermophilus CRISPR/Cas system provides immunity in Escherichia coli

(2011) Rimantas Sapranauskas et al.   NUCLEIC ACIDS RESEARCH

RNA-guided complex from a bacterial immune system enhances target recognition through seed sequence interactions

(2011) B. Wiedenheft et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

The CRISPR/Cas bacterial immune system cleaves bacteriophage and plasmid DNA

(2010) Josiane E. Garneau et al.   NATURE

Self versus non-self discrimination during CRISPR RNA-directed immunity

(2010) Luciano A. Marraffini et al.   NATURE

RNA-Guided RNA Cleavage by a CRISPR RNA-Cas Protein Complex

(2009) Caryn R. Hale et al.   CELL

Short motif sequences determine the targets of the prokaryotic CRISPR defence system

(2009)   MICROBIOLOGY-SGM

Structure of the Yeast DEAD Box Protein Mss116p Reveals Two Wedges that Crimp RNA

(2009) Mark Del Campo et al.   MOLECULAR CELL

I-TASSER server for protein 3D structure prediction

(2008) Yang Zhang   BMC BIOINFORMATICS

Small CRISPR RNAs Guide Antiviral Defense in Prokaryotes

(2008) S. J. J. Brouns et al.   SCIENCE

Function of the C-terminal Domain of the DEAD-box Protein Mss116p Analyzed in Vivo and in Vitro

(2007) Georg Mohr et al.   JOURNAL OF MOLECULAR BIOLOGY