Bringing immersive science to undergraduate laboratory courses using CRISPR gene knockouts in frogs and butterflies

An undergraduate laboratory experience using CRISPR-Cas9 technology to deactivate green fluorescent protein expression in Escherichia coli

(2019) Jay N. Pieczynski et al.   BIOCHEMISTRY AND MOLECULAR BIOLOGY EDUCATION

Single-dose CRISPR–Cas9 therapy extends lifespan of mice with Hutchinson–Gilford progeria syndrome

(2019) Ergin Beyret et al.   NATURE MEDICINE

Activation of butterfly eyespots by Distal-less is consistent with a reaction-diffusion process

(2019) Heidi Connahs et al.   DEVELOPMENT

Gephebase, a database of genotype–phenotype relationships for natural and domesticated variation in Eukaryotes

(2019) Virginie Courtier-Orgogozo et al.   NUCLEIC ACIDS RESEARCH

A Protocol for Laboratory Housing of Turquoise Killifish (Nothobranchius furzeri)

(2018) Joanna Dodzian et al.   Jove-Journal of Visualized Experiments

CRISPR Gene Editing in Yeast: An Experimental Protocol for an Upper-Division Undergraduate Laboratory Course

(2018) Nitasha Sehgal et al.   BIOCHEMISTRY AND MOLECULAR BIOLOGY EDUCATION

Seasonal changes in diet and chemical defense in the Climbing Mantella frog (Mantella laevigata)

(2018) Nora A. Moskowitz et al.   PLoS One

Shock greets claim of CRISPR-edited babies

(2018) Dennis Normile   SCIENCE

Tissue-Specific Gene Inactivation inXenopus laevis: Knockout oflhx1in the Kidney with CRISPR/Cas9

(2017) Bridget D. DeLay et al.   GENETICS

Genetic Basis of Melanin Pigmentation in Butterfly Wings

(2017) Linlin Zhang et al.   GENETICS

Ultrabithorax and abdominal-A specify the abdominal appendage in a dosage-dependent manner in silkworm, Bombyx mori

(2017) X L Tong et al.   HEREDITY

Single-Dose Gene-Replacement Therapy for Spinal Muscular Atrophy

(2017) Jerry R. Mendell et al.   NEW ENGLAND JOURNAL OF MEDICINE

Xenbase: a genomic, epigenomic and transcriptomic model organism database

(2017) Kamran Karimi et al.   NUCLEIC ACIDS RESEARCH

Macroevolutionary shifts ofWntAfunction potentiate butterfly wing-pattern diversity

(2017) Anyi Mazo-Vargas et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Single master regulatory gene coordinates the evolution and development of butterfly color and iridescence

(2017) Linlin Zhang et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

How should the applications of genome editing be assessed and regulated?

(2017) Robin Fears et al.   eLife

Tissue-Specific Gene Inactivation inXenopus laevis: Knockout oflhx1in the Kidney with CRISPR/Cas9

(2017) Bridget D. DeLay et al.   GENETICS

Genetic Basis of Melanin Pigmentation in Butterfly Wings

(2017) Linlin Zhang et al.   GENETICS

An undergraduate laboratory class using CRISPR/Cas9 technology to mutate drosophila genes

(2016) Vanesa Adame et al.   BIOCHEMISTRY AND MOLECULAR BIOLOGY EDUCATION

Maximizing mutagenesis with solubilized CRISPR-Cas9 ribonucleoprotein complexes

(2016) Alexa Burger et al.   DEVELOPMENT

Designing babies through gene editing: science or science fiction?

(2016) A. Cecile J.W. Janssens   GENETICS IN MEDICINE

CRISPR/Cas9 mediated knockout of the abdominal-A homeotic gene in the global pest, diamondback moth (Plutella xylostella)

(2016) Yuping Huang et al.   INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY

CRISPR/Cas9-mediated targeted gene mutagenesis inSpodoptera litura

(2016) Hong-Lun Bi et al.   Insect Science

Molecular logic behind the three-way stochastic choices that expand butterfly colour vision

(2016) Michael Perry et al.   NATURE

The Monarch Initiative: an integrative data and analytic platform connecting phenotypes to genotypes across species

(2016) Christopher J. Mungall et al.   NUCLEIC ACIDS RESEARCH

Genome editing in butterflies reveals that spalt promotes and Distal-less represses eyespot colour patterns

(2016) Linlin Zhang et al.   Nature Communications

CRISPR/Cas9 genome editing – new and old ethical issues arising from a revolutionary technology

(2016) Martina Baumann   NanoEthics

CRISPR-Cas Gene Editing to Cure Serious Diseases: Treat the Patient, Not the Germ Line

(2015) Ante S. Lundberg et al.   AMERICAN JOURNAL OF BIOETHICS

“Editing” Genes: A Case Study About How Language Matters in Bioethics

(2015) Meaghan O'Keefe et al.   AMERICAN JOURNAL OF BIOETHICS

Efficient CRISPR-mediated gene targeting and transgene replacement in the beetle Tribolium castaneum

(2015) A. F. Gilles et al.   DEVELOPMENT

DNA-free genome editing in plants with preassembled CRISPR-Cas9 ribonucleoproteins

(2015) Je Wook Woo et al.   NATURE BIOTECHNOLOGY

Targeted gene disruption in Xenopus laevis using CRISPR/Cas9

(2015) Fengqin Wang et al.   Cell and Bioscience

CCTop: An Intuitive, Flexible and Reliable CRISPR/Cas9 Target Prediction Tool

(2015) Manuel Stemmer et al.   PLoS One

Cas-OFFinder: a fast and versatile algorithm that searches for potential off-target sites of Cas9 RNA-guided endonucleases

(2014) S. Bae et al.   BIOINFORMATICS

Assessment of Course-Based Undergraduate Research Experiences: A Meeting Report

(2014) Lisa Corwin Auchincloss et al.   CBE-Life Sciences Education

Tissue-specific genome editing in Ciona embryos by CRISPR/Cas9

(2014) A. Stolfi et al.   DEVELOPMENT

Gene knockout by targeted mutagenesis in a hemimetabolous insect, the two-spotted cricket Gryllus bimaculatus, using TALENs

(2014) Takahito Watanabe et al.   METHODS

Ongoing invasions of the African clawed frog, Xenopus laevis: a global review

(2012) G. J. Measey et al.   BIOLOGICAL INVASIONS

Fostering Critical Thinking, Reasoning, and Argumentation Skills through Bioethics Education

(2012) Jeanne Ting Chowning et al.   PLoS One

Paralysis and delayed Z-disc formation in the Xenopus tropicalis unc45b mutant dicky ticker

(2010) Timothy J Geach et al.   BMC DEVELOPMENTAL BIOLOGY

The secreted integrin ligand nephronectin is necessary for forelimb formation in Xenopus tropicalis

(2010) Anita Abu-Daya et al.   DEVELOPMENTAL BIOLOGY

Gene therapy for red–green colour blindness in adult primates

(2009) Katherine Mancuso et al.   NATURE