Review
Medicine, Research & Experimental
Baris Cerci, Ihsan Alp Uzay, Mustafa Kemal Kara, Pervin Dincer
Summary: Treatment of genetic disorders through genomic manipulation has long been a goal for researchers. Recent advancements in the CRISPR/Cas systems have provided a more efficient and practical approach for editing the genome and have accelerated research on their potential use in treating genetic diseases. This review summarizes clinical trials and promising preclinical research on CRISPR/Cas-mediated treatment of various genetic disorders, and discusses the future implications of genome editing using CRISPR/Cas in the field of genetic diseases.
Review
Biochemistry & Molecular Biology
Mouraya Hussein, Mariano A. Molina, Ben Berkhout, Elena Herrera-Carrillo
Summary: HIV and AIDS are a global health burden, with no effective vaccine or cure currently available. However, CRISPR-Cas gene editing technology holds promise in the treatment of HIV.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Plant Sciences
Olga Kershanskaya, Gulvira L. Yessenbaeva, Darya S. Nelidova, Aizhan N. Karabekova, Zarina N. Sadullaeva
Summary: The CRISPR/Cas9 technology has the potential to improve crop breeding efficiency and antiviral defense, and its application in barley breeding has shown promising results.
PHYSIOLOGIA PLANTARUM
(2022)
Review
Biochemistry & Molecular Biology
Magdalena Hryhorowicz, Daniel Lipinski, Joanna Zeyland
Summary: The bacteria-derived CRISPR/Cas system is a versatile tool for genome engineering, with wide applications in various fields. However, challenges like immunological reactions and off-target effects remain. This review discusses the current classification of CRISPR systems, new genome-editing technologies, recent applications, as well as limitations, ethical issues, and challenges associated with the CRISPR/Cas system.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Biochemistry & Molecular Biology
Simran Bhatia, Sudesh Kumar Pooja, Sudesh Kumar Yadav
Summary: The CRISPR-Cas system is an adaptive immune response mechanism in prokaryotes that integrates short sequences of the target genome (spacers) into the CRISPR locus. CRISPR-Cas, classified based on Cas proteins, uses programmable RNAs to target DNA sequences and has become a powerful technique in genome editing. This article discusses the evolution, classification, design, and molecular mechanism of CRISPR-Cas systems, as well as their applications in agriculture and anticancer therapy. It also briefly explores the role of CRISPR and its Cas systems in the diagnosis and prevention of COVID-19, and discusses the challenges and potential solutions in existing CRISPR-Cas technologies.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Review
Biochemistry & Molecular Biology
Muhammad Naeem, Mubasher Zahir Hoque, Muhammad Ovais, Chanbasha Basheer, Irshad Ahmad
Summary: The innovative CRISPR-Cas technology has enabled genetic engineers to manipulate genomes effectively, but barriers in clinical applications remain, including efficient delivery and off-target effects. Various viral and non-viral vectors are designed to address these issues, but challenges such as immune response and lack of specificity persist.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Zheng Li, Zhenhui Zhong, Zhongshou Wu, Patrick Pausch, Basem Al-Shayeb, Jasmine Amerasekera, Jennifer A. Doudna, Steven E. Jackson
Summary: Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) systems are important tools for plant genome engineering. In this study, the hypercompact Cas phi nuclease is shown to generate stably inherited gene edits in Arabidopsis. The Cas phi protein variants, vCas phi and nCas phi, exhibit higher editing efficiency and no off-target editing was observed. The characteristics of Cas phi, such as its small size, T-rich minimal protospacer adjacent motif (PAM), and wide range of working temperatures, make it an excellent supplement to existing plant genome editing systems.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Review
Microbiology
Ruben D. Arroyo-Olarte, Ricardo Bravo Rodriguez, Edgar Morales-Rios
Summary: CRISPR-Cas technology has revolutionized genome editing in eukaryotic organisms with its simplicity and programmability, but faces challenges in bacterial genome editing. Alternative approaches are being explored to overcome these issues and expand the application of genome editing in bacteria.
Review
Chemistry, Medicinal
Xuesong He, Xiao Xue Zeng
Summary: This article provides a brief overview of immunotherapeutic options for COVID-19 and discusses the potential of CRISPR Cas system technology in COVID-19 treatment. Immunotherapeutics may serve as a specific cure against COVID-19 by boosting human immune responses. Additionally, CRISPR Cas technology could improve existing cell-based therapeutics and potentially terminate infection by degrading viral RNA.
DRUG DESIGN DEVELOPMENT AND THERAPY
(2022)
Review
Environmental Sciences
Suvra Roy, Vikash Kumar, Bijay Kumar Behera, Janmejay Parhi, Sipra Mohapatra, Tapas Chakraborty, Basanta Kumar Das
Summary: Fisheries and aquaculture, as the fastest-growing food-producing sector, can benefit from genome editing technology like CRISPR/Cas9 for sustainable genetic improvement. However, there are technical, regulatory, and public challenges to be overcome.
FRONTIERS IN MARINE SCIENCE
(2022)
Review
Biochemistry & Molecular Biology
Hyun-Min Kim, Yebin Hong, Jiani Chen
Summary: CRISPR-Cas enables highly efficient genome editing, allowing the introduction of desired modifications such as mutations and deletions. C. elegans has emerged as a significant model organism for CRISPR-Cas, with numerous strategies established. However, the abundance of methods makes it challenging to choose the correct approach, highlighting the importance of selecting appropriate screening methods for genome modification planning.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Oncology
Imran Ansari, Animesh Chaturvedi, Deepak Chitkara, Saurabh Singh
Summary: The understanding of the relationship between epigenetic alterations and gene expression have opened up new therapeutic pathways for treating various diseases, including cancer. CRISPR/Cas and other genome editing technologies have shown potential in epigenome editing. This review outlines the epigenetic therapeutic approach using CRISPR/Cas for cancer therapy.
SEMINARS IN CANCER BIOLOGY
(2022)
Review
Genetics & Heredity
Chao Li, Wen Chu, Rafaqat Ali Gill, Shifei Sang, Yuqin Shi, Xuezhi Hu, Yuting Yang, Qamar U. Zaman, Baohong Zhang
Summary: In the past decade, significant progress has been made in developing versatile CRISPR/Cas nucleases and precise genome editors. Computer-assisted tools have played a crucial role in designing sgRNA and analyzing CRISPR/Cas-induced mutations. This review provides insights into the different features of CRISPR/Cas tools, the usage of computer-assisted tools, and the limitations and suggestions for further optimization.
GENOMICS PROTEOMICS & BIOINFORMATICS
(2023)
Review
Medicine, Research & Experimental
Sajad Najafi, Shing Cheng Tan, Shahin Aghamiri, Pourya Raee, Zahra Ebrahimi, Zahra Kargar Jahromi, Yazdan Rahmati, Javid Sadri Nahand, Ahmad Piroozmand, Vahid Jajarmi, Hamed Mirzaei
Summary: The article reviews the research progress of CRISPR-Cas technology in treating viral infections, discusses delivery strategies and challenges, and proposes future research directions.
BIOMEDICINE & PHARMACOTHERAPY
(2022)
Review
Biotechnology & Applied Microbiology
Tien Van Vu, Duong Thi Hai Doan, Jihae Kim, Yeon Woo Sung, Mil Thi Tran, Young Jong Song, Swati Das, Jae-Yean Kim
Summary: The ultimate goals of genetic engineering are to achieve gene editing and allele introgression with absolute precision and control. Various approaches have been developed in plants for precision genome editing, with significant advancements in HR-mediated editing frequencies through CRISPR/Cas technology. Despite this progress, further research is needed for commercially viable applications of precise genome editing, potentially requiring alternative innovative methods.
PLANT BIOTECHNOLOGY JOURNAL
(2021)
