Article
Biochemistry & Molecular Biology
Xiaojing Wang, Linchuan Ma, Jingmin Li, Fanzhen Kong
Summary: Dysregulation of the dorsal raphe nucleus (DRN) has been found to be associated with cognitive and arousal impairments in post-traumatic stress disorder (PTSD). In this study, a rat model of PTSD was established using acute exposure to single prolonged stress (SPS), and the effects of the cell-cycle signaling pathway in DRN were examined. The results showed increased apoptosis in DRN after SPS, and activation of the CDK4/CyclinD1-pRB-E2F1 pathway was involved in DRN apoptosis. Treatment with the CDK4 inhibitor Abemaciclib attenuated apoptosis and rescued spatial learning and memory abilities in SPS rats, suggesting that this pathway may be part of the pathogenesis of PTSD.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Review
Biochemistry & Molecular Biology
Silvia Salvatori, Irene Marafini, Federica Laudisi, Giovanni Monteleone, Carmine Stolfi
Summary: H. pylori infection contributes to the onset and development of gastric cancer through the mechanisms of chronic inflammation and DNA damage to gastric epithelial cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Medicine, General & Internal
Yoshiaki Usui, Yukari Taniyama, Mikiko Endo, Yuriko N. Koyanagi, Yumiko Kasugai, Isao Oze, Hidemi Ito, Issei Imoto, Tsutomu Tanaka, Masahiro Tajika, Yasumasa Niwa, Yusuke Iwasaki, Tomomi Aoi, Nozomi Hakozaki, Sadaaki Takata, Kunihiko Suzuki, Chikashi Terao, Masanori Hatakeyama, Makoto Hirata, Kokichi Sugano, Teruhiko Yoshida, Yoichiro Kamatani, Hidewaki Nakagawa, Koichi Matsuda, Yoshinori Murakami, Amanda B. Spurdle, Keitaro Matsuo, Yukihide Momozawa
Summary: This study reveals that certain genetic variants associated with Helicobacter pylori infection can increase the risk of gastric cancer. Individuals carrying these genetic variants and infected with H. pylori have an even higher risk of developing gastric cancer.
NEW ENGLAND JOURNAL OF MEDICINE
(2023)
Article
Biochemistry & Molecular Biology
Ying Zhou, Rishun Fu, Mei Yang, Weihuang Liu, Zan Tong
Summary: Lycopene, a natural carotenoid, inhibits the growth and induces apoptosis in gastric cancer cells. It decreases CCNE1 expression and increases the levels of TP53, making it a potential target therapy reagent for gastric cancer.
JOURNAL OF NUTRITIONAL BIOCHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
So Dam Lee, Haengdueng Jeong, Bo Ram Hwang, Byeong Min Yu, Yejin Cho, Ki Teak Nam, Hyunki Kim, Yong Chan Lee
Summary: Strains of Helicobacter pylori positive for CagA may contribute to the development of gastric cancer through the induction of CK2 activation and CK28 degradation. The downregulation of CK28 and subsequent activation of AKT and Snail signaling pathways may play a crucial role in the epithelial-to-mesenchymal transition (EMT) in gastric cancer. CK2 could be a potential molecular target for the treatment of H. pylori-infected gastric cancer.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2023)
Article
Cell Biology
XiaoYi Zhang, Chao Li, Dingyu Chen, XiaoFeng He, Yan Zhao, LiYa Bao, Qingrong Wang, JianJiang Zhou, Yuan Xie
Summary: This study demonstrates that CagA can promote the invasion and migration of gastric cancer cells by activating the NLRP3 inflammasome pathway. Inhibition of ROS effectively blocks NLRP3 activation and pyroptosis. Silencing of NLRP3 reduces the effects of CagA on gastric cancer cell migration and invasion.
INFLAMMATION RESEARCH
(2022)
Review
Oncology
Shom Goel, Johann S. Bergholz, Jean J. Zhao
Summary: Dysregulation of CDK4 and CDK6 promotes the growth and survival of several cancer types. CDK4/6 inhibitors have become the standard of care for advanced hormone receptor-positive breast cancer. Better understanding of their mechanisms of action and exploring new therapeutic opportunities are crucial.
NATURE REVIEWS CANCER
(2022)
Article
Pharmacology & Pharmacy
Gen Li, Tianquan Yang, Yanling Chen, Jianping Bao, Di Wu, Xiaohan Hu, Chenxi Feng, Lixiao Xu, Mei Li, Gang Li, Meifang Jin, Yunyun Xu, Rui Zhang, Guanghui Qian, Jian Pan
Summary: The study revealed that USP5 plays a critical role in the tumorigenesis and progression of GBM by stabilizing CyclinD1 protein levels. Knockdown of USP5 inhibited tumor growth, suggesting that targeting USP5 could be a potential therapeutic strategy for GBM.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Laura Francesca Pisani, Gian Eugenio Tontini, Carmine Gentile, Beatrice Marinoni, Isabella Teani, Nicoletta Nandi, Pasquale Creo, Emanuele Asti, Luigi Bonavina, Maurizio Vecchi, Luca Pastorelli
Summary: IL-33 has antiproliferative and proapoptotic effects on gastric cancer cell lines, while it stimulates proliferation and reduces apoptosis in normal epithelial cell lines. These effects may be mediated through the regulation of cell cycle gene expression.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biotechnology & Applied Microbiology
Zongjiao Ren, Jiayi Li, Xianhong Du, Wenjing Shi, Fulai Guan, Xiaochen Wang, Linjing Wang, Hongyan Wang
Summary: This study investigated the mechanism of gastric cancer caused by H. pylori infection. The results showed that PGRN was upregulated in response to H. pylori infection, and it promoted cell proliferation and cell cycle progression through CDK4. The PI3K/Akt signaling pathway was also involved in the regulation of CDK4 by PGRN.
JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Article
Gastroenterology & Hepatology
Jin Zhou, Zhong Wu, Zhouwei Zhang, Louisa Goss, James McFarland, Ankur Nagaraja, Yingtian Xie, Shengqing Gu, Ke Peng, Yong Zeng, Xiaoyang Zhang, Henry Long, Hiroshi Nakagawa, Anil Rustgi, J. Alan Diehl, Matthew Meyerson, Kwok-Kin Wong, Adam Bass
Summary: This study revealed a subset of oesophageal squamous cell carcinomas with dual dependence on ERBB3 and ERBB2, demonstrating the efficacy of combined pan-ERBB and CDK4/6 inhibition in vitro and in vivo. The potential for KLF5 expression as a candidate biomarker to guide the use of these agents was also highlighted, providing a rationale for the development of this combination therapy in squamous cancers.
Review
Multidisciplinary Sciences
Anne Fassl, Yan Geng, Piotr Sicinski
Summary: Cyclin-dependent kinases 4 and 6, along with their activation partners, D-type cyclins, play a crucial role in connecting the extracellular environment with the cell cycle machinery. Inhibiting CDK4/6 has shown success in treating hormone receptor-positive breast cancers and may have potential applications in other tumor types. Recent research has shown that CDK4/6 inhibition affects various cellular functions, including tumor cell metabolism and anti-tumor immunity.
Article
Cell Biology
Zhonghua Ma, Xiangyu Gao, You Shuai, Xiaolong Wu, Yan Yan, Xiaofang Xing, Jiafu Ji
Summary: Linc01503 is significantly upregulated in GC and closely associated with patient survival. The transcription factor EGR1 activates linc01503 transcription. Knockdown of linc01503 leads to apoptosis activation and G1/G0 phase arrest in GC. Linc01503 interacts with histone modification enzymes EZH2 and LSD1 to silence DUSP5/CDKN1A expression in GC, mediating cell cycle progression and tumorigenesis.
CELL PROLIFERATION
(2021)
Article
Oncology
Craig M. Goodwin, Andrew M. Waters, Jennifer E. Klomp, Sehrish Javaid, Kirsten L. Bryant, Clint A. Stalnecker, Kristina Drizyte-Miller, Bjoern Papke, Runying Yang, Amber M. Amparo, Irem Ozkan-Dagliyan, Elisa Baldelli, Valerie Calvert, Mariaelena Pierobon, Jessica A. Sorrentino, Andrew P. Beelen, Natalie Bublitz, Mareen Luethen, Kris C. Wood, Emanuel F. Petricoin III, Christine Sers, Autumn J. McRee, Adrienne D. Cox, Channing J. Der
Summary: Mutation of CDKN2A and activation of KRAS are crucial for the development and malignant growth of PDAC. Combination treatment with CDK4/6 and ERK-MAPK inhibitors synergistically suppresses the growth of PDAC cells and organoids by blocking compensatory upregulation of signaling pathways. CRISPR-Cas9 screening and protein activity mapping uncover novel combinations that enhance the potency of CDK4/6 inhibitors and overcome drug-induced compensations in PDAC.
Article
Cell Biology
Zilu Chen, Liyue Yuan, Xiaopeng Li, Junhui Yu, Zhengshui Xu
Summary: This study demonstrates that BMP2 acts as a tumor suppressor in gastric cancer cells by downregulating EZH2 and H3K27me3 through the non-SMAD BMP pathway, suggesting that BMP2 might be a potential therapeutic target for gastric cancer treatment.
Article
Biochemistry & Molecular Biology
Liang Qiao, Zheng-Wen Yan, Gao Xiong, You-jin Hao, Ri-xin Wang, Hai Hu, Jiang-bo Song, Xiao-ling Tong, Lin-rong Che, Song-zhen He, Bin Chen, James Mallet, Cheng Lu, Fang-yin Dai
INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY
(2020)
Article
Multidisciplinary Sciences
Guihua Jiang, Jiangbo Song, Hai Hu, Xiaoling Tong, Fangyin Dai
ROYAL SOCIETY OPEN SCIENCE
(2020)
Article
Oncology
Jiang-Bo Song, Kai-Ge Hao, Xin Chen, Yan-Hua Zhang, Zi-Lin Cheng, Shuang Mao, Yong-Xi Tang, Mao-Ling Tong, Fang-Yin Dai
ANNALS OF TRANSLATIONAL MEDICINE
(2020)
Article
Entomology
Jiang-Wen Luo, Er-Xia An, Ya-Ru Lu, Ling Yang, Ting-Ting Gai, Song-Zhen He, Song-Yuan Wu, Hai Hu, Chun-Lin Li, Cheng Lu, Xiao-Ling Tong, Fang-Yin Dai
Summary: The study revealed the molecular basis of silkworm re(l) mutant formation, identifying two genes that control egg color and embryo mortality, with BmSema1a gene being essential for insect embryo development.
Article
Agriculture, Dairy & Animal Science
Chunlin Li, Weidong Zuo, Xiaoling Tong, Minjin Han, Rui Gao, Hai Hu, Kunpeng Lu, Yue Luan, Bili Zhang, Yanyu Liu, Fangyin Dai
Summary: Breeding or genetic improvement plays a crucial role in modern agriculture and animal production by focusing on economically important traits. A study on silkworm genetic improvement identified 24 genomic regions with strong selection signals, including genes related to neural signal response, suggesting that improvement may have impacted the nervous system of the silkworm. This research provides new insights into the genetic basis underlying the genetic improvement of silkworms and possibly other species.
JOURNAL OF ANIMAL BREEDING AND GENETICS
(2021)
Article
Entomology
Zhan-Feng Ye, Pan Zhang, Ting-Ting Gai, Jing-Hou Lou, Fang-Yin Dai, Xiao-Ling Tong
Summary: The research found that the odd-skipped family genes, including sister of odd and bowl gene, play crucial roles in insect wing morphogenesis, with their loss leading to abnormal wing development and indistinct wing veins.
Article
Biochemical Research Methods
Chunyan Fang, Zhanfeng Ye, Tingting Gai, Kunpeng Lu, Fangyin Dai, Cheng Lu, Xiaoling Tong
Summary: Proteomic analysis of silkworm wings revealed that cuticular proteins and fatty metabolism enzymes are the main abnormally expressed proteins in the pupal wings of the mutant, leading to curly and shrunken wings after moth transformation.
JOURNAL OF PROTEOMICS
(2021)
Article
Biotechnology & Applied Microbiology
Yun-long Zou, Ai-jun Ye, Shuo Liu, Wen-tao Wu, Li-feng Xu, Fang-yin Dai, Xiao-ling Tong
Summary: The study investigated the use of T7 promoter to modify sgRNAs structure for efficient mutagenesis in silkworm. Results showed that sgRNAs with 5' GG mismatches can still induce mutations in vivo, with one sgRNA even outperforming conventional sgRNAs with perfectly matched 5' GG residues. The mutations generated by sgRNAs with 5' GG mismatches are also heritable, allowing for the successful generation of null mutants in the silkworm after one generation.
Article
Entomology
Lulu Liu, Pan Zhang, Qiang Gao, Xiaoge Feng, Lan Han, Fengbin Zhang, Yanmin Bai, Minjin Han, Hai Hu, Fangyin Dai, Gaojun Zhang, Xiaoling Tong
Summary: This study compared the differentially expressed miRNAs in bivoltine silkworm embryos incubated at diapause- and non-diapause-inducing temperatures, revealing that miRNAs respond to temperature changes and play a crucial role in regulating circadian genes during diapause induction. Through transcriptome sequencing and KEGG analysis, the study identified known and novel miRNAs, highlighting their involvement in metabolism, phototransduction, and hormone biosynthesis. The results suggest that microRNAs are important regulators in the diapause induction process across species.
Article
Developmental Biology
Chunyan Fang, Yaqun Xin, Tao Sun, Antonia Monteiro, Zhanfeng Ye, Fangyin Dai, Cheng Lu, Xiaoling Tong
Summary: The long-standing view that insect forewings develop independently of Hox genes is challenged by this study. The researchers show that the Hox gene Antennapedia (Antp) plays a role in wing development in Bombyx, Drosophila, and Tribolium by regulating the expression of specific genes involved in wing growth and cuticle formation.
Article
Entomology
Yun-Long Zou, Xin Ding, Li Zhang, Li-Feng Xu, Shu-Bo Liang, Hai Hu, Fang-Yin Dai, Xiao-Ling Tong
Summary: In this study, the genetic basis and regulatory mechanisms underlying wing size and shape development were investigated through the study of the micropterous mutant in the silkworm and Drosophila. The Bmmp gene responsible for the mp phenotype was identified and found to be functionally conserved in Drosophila. The findings contribute to our understanding of insect wing development mechanisms and provide potential targets for pest control.
Article
Biochemistry & Molecular Biology
Zhengwen Yan, Xiaoling Tong, Gao Xiong, Weike Yang, Kunpeng Lu, Yajie Yuan, Minjin Han, Hai Hu, Wei Wei, Fangyin Dai
Summary: Insect cuticle plays a critical role in the adaptability of insects to the environment and their resistance to insecticides. This study focused on the structure and protein components of the cuticle at each developmental stage of the silkworm (Bombyx mori). The researchers found that the processes of cuticle formation were similar across the larval, pupal, and adult stages, and a large portion of the expressed cuticular protein (CP) genes were shared among these stages. However, there were also stage-specific CP genes expressed in each cuticle. The types and levels of CP genes may be important factors determining the properties of each cuticular layer. This study provides a comprehensive understanding of cuticle morphology and protein components, and contributes to future research on the molecular mechanisms underlying cuticle formation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Multidisciplinary Sciences
Xiaoling Tong, Min-Jin Han, Kunpeng Lu, Shuaishuai Tai, Shubo Liang, Yucheng Liu, Hai Hu, Jianghong Shen, Anxing Long, Chengyu Zhan, Xin Ding, Shuo Liu, Qiang Gao, Bili Zhang, Linli Zhou, Duan Tan, Yajie Yuan, Nangkuo Guo, Yan-Hong Li, Zhangyan Wu, Lulu Liu, Chunlin Li, Yaru Lu, Tingting Gai, Yahui Zhang, Renkui Yang, Heying Qian, Yanqun Liu, Jiangwen Luo, Lu Zheng, Jinghou Lou, Yunwu Peng, Weidong Zuo, Jiangbo Song, Songzhen He, Songyuan Wu, Yunlong Zou, Lei Zhou, Lan Cheng, Yuxia Tang, Guotao Cheng, Lianwei Yuan, Weiming He, Jiabao Xu, Tao Fu, Yang Xiao, Ting Lei, Anying Xu, Ye Yin, Jian Wang, Antonia Monteiro, Eric Westhof, Cheng Lu, Zhixi Tian, Wen Wang, Zhonghuai Xiang, Fangyin Dai
Summary: In this study, a super pangenome assembled from long-read sequences of 545 silkworms is described. The researchers identified sets of genes that were naturally or artificially selected and found a high density of genomic variants in the silkworm population. They also discovered thousands of new genes and structure variations that may contribute to the artificial selection of silkworm for silk production and adaptation. The findings provide valuable genomic resources for functional genomics studies and breeding improvement in silkworm.
NATURE COMMUNICATIONS
(2022)
Article
Genetics & Heredity
Minjin Han, Jianyu Ren, Haipeng Guo, Xiaoling Tong, Hai Hu, Kunpeng Lu, Zongrui Dai, Fangyin Dai
Summary: By studying the mutation rate and spectrum of the domestic silkworm under normal and two temperature stress conditions, it was found that the single-nucleotide mutation rate under normal temperature rearing condition was 1.5-fold higher than in previously studied insects. Moreover, the mutation rates of the silkworm under stress conditions were significantly higher than in normal conditions. Furthermore, the mutation rate varied less in gene regions under normal and temperature stresses. These findings expand the diversity of the mutation rate among eukaryotes and have implications for evolutionary analysis.
Article
Biochemistry & Molecular Biology
Bili Zhang, Chunlin Li, Yue Luan, Yaru Lu, Hai Hu, Yanyu Liu, Kunpeng Lu, Guizheng Zhang, Fangyin Dai, Xiaoling Tong
Summary: In this study, nine HEX genes in Bombyx mori were identified and their distinct expression patterns were analyzed. The chitooligosaccharidolytic beta-N-acetylglucosaminidase (BmChiNAG) gene was found to be highly expressed in chitin-rich tissues and played a crucial role in molting and wing development in silkworms.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
