Effective temperature shift strategy development and scale confirmation for simultaneous optimization of protein productivity and quality in Chinese hamster ovary cells

Process intensification in fed-batch production bioreactors using non-perfusion seed cultures

(2019) Andrew Yongky et al.   mAbs

Industrial bioprocessing perspectives on managing therapeutic protein charge variant profiles

(2018) Stanley Chung et al.   BIOTECHNOLOGY AND BIOENGINEERING

Identification of process conditions influencing protein aggregation in Chinese hamster ovary cell culture

(2018) Albert J. Paul et al.   BIOTECHNOLOGY AND BIOENGINEERING

Mild hypothermia upregulates myc and xbp1s expression and improves anti-TNFα production in CHO cells

(2018) Mauro Torres et al.   PLoS One

Systematic development of temperature shift strategies for Chinese hamster ovary cells based on short duration cultures and kinetic modeling

(2018) Jianlin Xu et al.   mAbs

A semi-empirical glycosylation model of a camelid monoclonal antibody under hypothermia cell culture conditions

(2017) Hengameh Aghamohseni et al.   JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY

Climbing the mountain: experimental design for the efficient optimization of stem cell bioprocessing

(2017) Derek Toms et al.   Journal of Biological Engineering

Biphasic cultivation strategy to avoid Epo-Fc aggregation and optimize protein expression

(2016) Christian Kaisermayer et al.   JOURNAL OF BIOTECHNOLOGY

Concentrated fed-batch cell culture increases manufacturing capacity without additional volumetric capacity

(2016) William C. Yang et al.   JOURNAL OF BIOTECHNOLOGY

Effect of Temperature Downshift on the Transcriptomic Responses of Chinese Hamster Ovary Cells Using Recombinant Human Tissue Plasminogen Activator Production Culture

(2016) Andrea Bedoya-López et al.   PLoS One

How does mild hypothermia affect monoclonal antibody glycosylation?

(2015) Si Nga Sou et al.   BIOTECHNOLOGY AND BIOENGINEERING

Culture temperature modulates monoclonal antibody charge variation distribution in Chinese hamster ovary cell cultures

(2015) Xintao Zhang et al.   BIOTECHNOLOGY LETTERS

Effect of temperature shift on levels of acidic charge variants in IgG monoclonal antibodies in Chinese hamster ovary cell culture

(2015) Shohei Kishishita et al.   JOURNAL OF BIOSCIENCE AND BIOENGINEERING

The choice of mammalian cell host and possibilities for glycosylation engineering

(2014) Michael Butler et al.   CURRENT OPINION IN BIOTECHNOLOGY

Differential Effect of Culture Temperature and Specific Growth Rate on CHO Cell Behavior in Chemostat Culture

(2014) Mauricio Vergara et al.   PLoS One

Asparagine Deamidation Dependence on Buffer Type, pH, and Temperature

(2013) Amanda L. Pace et al.   JOURNAL OF PHARMACEUTICAL SCIENCES

CHO Quasispecies—Implications for Manufacturing Processes

(2013) Florian Wurm   Processes

CHO cells in biotechnology for production of recombinant proteins: current state and further potential

(2011) Jee Yon Kim et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Mammalian cell protein expression for biopharmaceutical production

(2011) Jianwei Zhu   BIOTECHNOLOGY ADVANCES

Culture temperature modulates aggregation of recombinant antibody in cho cells

(2011) Natalia Gomez et al.   BIOTECHNOLOGY AND BIOENGINEERING

Process analysis of reduced specific productivity of TNFR-Fc in Chinese hamster ovary cells at high cell density

(2011) Tian-Ci Kou et al.   PROCESS BIOCHEMISTRY

Effect of culture temperature on TNFR-Fc productivity in recombinant glutamine synthetase-chinese hamster ovary cells

(2010) Li Fan et al.   BIOTECHNOLOGY LETTERS

Dynamic model of CHO cell metabolism

(2010) Ryan P. Nolan et al.   METABOLIC ENGINEERING

Effects of culture conditions onN-glycolylneuraminic acid (Neu5Gc) content of a recombinant fusion protein produced in CHO cells

(2009) Michael C. Borys et al.   BIOTECHNOLOGY AND BIOENGINEERING

Enhanced interferon-β production by CHO cells through elevated osmolality and reduced culture temperature

(2009) Young Kue Han et al.   BIOTECHNOLOGY PROGRESS

Transcriptome and proteome analysis of Chinese hamster ovary cells under low temperature and butyrate treatment

(2009) Anne Kantardjieff et al.   JOURNAL OF BIOTECHNOLOGY

Effect of culture temperature on erythropoietin production and glycosylation in a perfusion culture of recombinant CHO cells

(2008) Woo Suk Ahn et al.   BIOTECHNOLOGY AND BIOENGINEERING

Comparative transcriptome analysis to unveil genes affecting recombinant protein productivity in mammalian cells

(2008) Joon Chong Yee et al.   BIOTECHNOLOGY AND BIOENGINEERING

Mild Hypothermia Improves Transient Gene Expression Yields Several Fold in Chinese Hamster Ovary Cells

(2008) S. Wulhfard et al.   BIOTECHNOLOGY PROGRESS

Bioprocess optimization using design-of-experiments methodology

(2008) Carl-Fredrik Mandenius et al.   BIOTECHNOLOGY PROGRESS

Metabolic Rates, Growth Phase, and mRNA Levels Influence Cell-Specific Antibody Production Levels from In Vitro-Cultured Mammalian Cells at Sub-Physiological Temperatures

(2008) Rosalyn J. Marchant et al.   MOLECULAR BIOTECHNOLOGY

Temperature Reduction in Cultures of hGM-CSF-expressing CHO Cells: Effect on Productivity and Product Quality

(2005) Mariela Bollati-Fogolín et al.   BIOTECHNOLOGY PROGRESS

Effect of Low Culture Temperature on Specific Productivity and Transcription Level of Anti-4-1BB Antibody in Recombinant Chinese Hamster Ovary Cells

(2003) Sung Kwan Yoon et al.   BIOTECHNOLOGY PROGRESS