Bacterial genome engineering and synthetic biology: combating pathogens

A highly precise and portable genome engineering method allows comparison of mutational effects across bacterial species

(2016) Ákos Nyerges et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Design and synthesis of a minimal bacterial genome

(2016) C. A. Hutchison et al.   SCIENCE

Genetic circuit design automation

(2016) A. A. K. Nielsen et al.   SCIENCE

Reinvigorating natural product combinatorial biosynthesis with synthetic biology

(2015) Eunji Kim et al.   Nature Chemical Biology

'Deadman' and 'Passcode' microbial kill switches for bacterial containment

(2015) Clement T Y Chan et al.   Nature Chemical Biology

Synthetic biology devices for in vitro and in vivo diagnostics

(2015) Shimyn Slomovic et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Temperate and lytic bacteriophages programmed to sensitize and kill antibiotic-resistant bacteria

(2015) Ido Yosef et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Implantable synthetic cytokine converter cells with AND-gate logic treat experimental psoriasis

(2015) Lina Schukur et al.   Science Translational Medicine

Tunable Riboregulator Switches for Post-transcriptional Control of Gene Expression

(2015) Malathy Krishnamurthy et al.   ACS Synthetic Biology

Bacterial Recombineering: Genome Engineering via Phage-Based Homologous Recombination

(2015) Gur Pines et al.   ACS Synthetic Biology

High-Efficiency Scarless Genetic Modification in Escherichia coli by Using Lambda Red Recombination and I-SceI Cleavage

(2014) Junjie Yang et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Toehold Switches: De-Novo-Designed Regulators of Gene Expression

(2014) Alexander A. Green et al.   CELL

Paper-Based Synthetic Gene Networks

(2014) Keith Pardee et al.   CELL

Bacteriophage-based synthetic biology for the study of infectious diseases

(2014) Robert J Citorik et al.   CURRENT OPINION IN MICROBIOLOGY

Bacterial Cellular Engineering by Genome Editing and Gene Silencing

(2014) Nobutaka Nakashima et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Sequence-specific antimicrobials using efficiently delivered RNA-guided nucleases

(2014) Robert J Citorik et al.   NATURE BIOTECHNOLOGY

Exploiting CRISPR-Cas nucleases to produce sequence-specific antimicrobials

(2014) David Bikard et al.   NATURE BIOTECHNOLOGY

Generalized bacterial genome editing using mobile group II introns and Cre-lox

(2013) P. J. Enyeart et al.   Molecular Systems Biology

Progress and pitfalls in Shigella vaccine research

(2013) Eileen M. Barry et al.   Nature Reviews Gastroenterology & Hepatology

Synthetic RNA Silencing of Actinorhodin Biosynthesis in Streptomyces coelicolor A3(2)

(2013) Gabriel C. Uguru et al.   PLoS One

A quorum-sensing inhibitor blocks Pseudomonas aeruginosa virulence and biofilm formation

(2013) C. T. O'Loughlin et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Advancing bacteriophage-based microbial diagnostics with synthetic biology

(2013) Timothy K. Lu et al.   TRENDS IN BIOTECHNOLOGY

Construction, characterization and preclinical evaluation of MTBVAC, the first live-attenuated M. tuberculosis-based vaccine to enter clinical trials

(2013) Ainhoa Arbues et al.   VACCINE

Genetically Programmable Pathogen Sense and Destroy

(2013) Saurabh Gupta et al.   ACS Synthetic Biology

Reprogramming Microbes to Be Pathogen-Seeking Killers

(2013) In Young Hwang et al.   ACS Synthetic Biology

Bile Acid Recognition by the Clostridium difficile Germinant Receptor, CspC, Is Important for Establishing Infection

(2013) Michael B. Francis et al.   PLoS Pathogens

Vaccines for typhoid fever and other salmonelloses

(2012) Laura B. Martin   CURRENT OPINION IN INFECTIOUS DISEASES

DksA-Dependent Resistance of Salmonella enterica Serovar Typhimurium against the Antimicrobial Activity of Inducible Nitric Oxide Synthase

(2012) Calvin A. Henard et al.   INFECTION AND IMMUNITY

Genome-wide enrichment screening reveals multiple targets and resistance genes for triclosan in Escherichia coli

(2012) Byung Jo Yu et al.   JOURNAL OF MICROBIOLOGY

The future of metabolic engineering and synthetic biology: Towards a systematic practice

(2012) Vikramaditya G. Yadav et al.   METABOLIC ENGINEERING

Synthetic biology and the development of tools for metabolic engineering

(2012) Jay D. Keasling   METABOLIC ENGINEERING

The next generation of bacteriophage therapy

(2011) Timothy K Lu et al.   CURRENT OPINION IN MICROBIOLOGY

Probing Cellular Processes with Oligo-Mediated Recombination and Using the Knowledge Gained to Optimize Recombineering

(2011) James A. Sawitzke et al.   JOURNAL OF MOLECULAR BIOLOGY

Smart medication through combination of synthetic biology and cell microencapsulation

(2011) Simon Ausländer et al.   METABOLIC ENGINEERING

Synthetic biology: New strategies for directing design

(2011) Sean A. Lynch et al.   METABOLIC ENGINEERING

Engineering microbes to sense and eradicate Pseudomonas aeruginosa, a human pathogen

(2011) N. Saeidi et al.   Molecular Systems Biology

Rapid and Highly Efficient Method for Scarless Mutagenesis within the Salmonella enterica Chromosome

(2011) Kathrin Blank et al.   PLoS One

Expression of Signal Transduction System Encoding Genes of Yersinia pseudotuberculosis IP32953 at 28°C and 3°C

(2011) Eveliina Palonen et al.   PLoS One

Synthetic Biology Moving into the Clinic

(2011) W. C. Ruder et al.   SCIENCE

Putative essential and core-essential genes in Mycoplasma genomes

(2011) Yan Lin et al.   Scientific Reports

Cloning and molecular analysis of a mannitol operon of phosphoenolpyruvate-dependent phosphotransferase (PTS) type from Vibrio cholerae O395

(2010) Sanath Kumar et al.   ARCHIVES OF MICROBIOLOGY

Scarless and sequential gene modification in Pseudomonas using PCR product flanked by short homology regions

(2010) Rubing Liang et al.   BMC MICROBIOLOGY

Control of Redox Balance by the Stringent Response Regulatory Protein Promotes Antioxidant Defenses ofSalmonella

(2010) Calvin A. Henard et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

How antibiotics kill bacteria: from targets to networks

(2010) Michael A. Kohanski et al.   NATURE REVIEWS MICROBIOLOGY

Site-specific chromosomal integration of large synthetic constructs

(2010) Thomas E. Kuhlman et al.   NUCLEIC ACIDS RESEARCH

Tracking, tuning, and terminating microbial physiology using synthetic riboregulators

(2010) J. M. Callura et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Engineered bacterial communication prevents Vibrio cholerae virulence in an infant mouse model

(2010) F. Duan et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Creation of a Bacterial Cell Controlled by a Chemically Synthesized Genome

(2010) D. G. Gibson et al.   SCIENCE

Fis Is Essential for Capsule Production in Pasteurella multocida and Regulates Expression of Other Important Virulence Factors

(2010) Jason A. Steen et al.   PLoS Pathogens

Gene doctoring: a method for recombineering in laboratory and pathogenic Escherichia coli strains

(2009) David J Lee et al.   BMC MICROBIOLOGY

Programming cells by multiplex genome engineering and accelerated evolution

(2009) Harris H. Wang et al.   NATURE

Mining microbial genomes for new natural products and biosynthetic pathways

(2008) G. L. Challis   MICROBIOLOGY-SGM

A fast, robust and tunable synthetic gene oscillator

(2008) Jesse Stricker et al.   NATURE