CORALINA: a universal method for the generation of gRNA libraries for CRISPR-based screening

Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System

(2015) Bernd Zetsche et al.   CELL

Intron targeting-mediated and endogenous gene integrity-maintaining knockin in zebrafish using the CRISPR/Cas9 system

(2015) Jia Li et al.   CELL RESEARCH

Efficient CRISPR-mediated gene targeting and transgene replacement in the beetle Tribolium castaneum

(2015) A. F. Gilles et al.   DEVELOPMENT

CRISPR/Cas9-mediated mutagenesis in the sea lamprey Petromyzon marinus: a powerful tool for understanding ancestral gene functions in vertebrates

(2015) T. Square et al.   DEVELOPMENT

Enzymatically Generated CRISPR Libraries for Genome Labeling and Screening

(2015) Andrew B. Lane et al.   DEVELOPMENTAL CELL

Single-step generation of rabbits carrying a targeted allele of the tyrosinase gene using CRISPR/Cas9

(2015) Arata HONDA et al.   EXPERIMENTAL ANIMALS

High-throughput screens in mammalian cells using the CRISPR-Cas9 system

(2015) Jingyu Peng et al.   FEBS Journal

Engineered CRISPR-Cas9 nucleases with altered PAM specificities

(2015) Benjamin P. Kleinstiver et al.   NATURE

Genetic modification of the diarrhoeal pathogen Cryptosporidium parvum

(2015) Sumiti Vinayak et al.   NATURE

BCL11A enhancer dissection by Cas9-mediated in situ saturating mutagenesis

(2015) Matthew C. Canver et al.   NATURE

In vivo genome editing using Staphylococcus aureus Cas9

(2015) F. Ann Ran et al.   NATURE

Functional annotation of native enhancers with a Cas9–histone demethylase fusion

(2015) Nicola A Kearns et al.   NATURE METHODS

Rapid and efficient one-step generation of paired gRNA CRISPR-Cas9 libraries

(2015) Joana A. Vidigal et al.   Nature Communications

Use of the CRISPR/Cas9 system as an intracellular defense against HIV-1 infection in human cells

(2015) Hsin-Kai Liao et al.   Nature Communications

Synthesis of an arrayed sgRNA library targeting the human genome

(2015) Tobias Schmidt et al.   Scientific Reports

Brave new epigenomes: the dawn of epigenetic engineering

(2015) Anna Köferle et al.   Genome Medicine

Use of the CRISPR/Cas9 System to Produce Genetically Engineered Pigs from In Vitro-Derived Oocytes and Embryos1

(2014) Kristin M. Whitworth et al.   BIOLOGY OF REPRODUCTION

CRISPR/Cas9-mediated gene knockout in the ascidianCiona intestinalis

(2014) Haruka Sasaki et al.   DEVELOPMENT GROWTH & DIFFERENTIATION

Conditional Knockouts Generated by Engineered CRISPR-Cas9 Endonuclease Reveal the Roles of Coronin in C. elegans Neural Development

(2014) Zhongfu Shen et al.   DEVELOPMENTAL CELL

Genome-wide binding of the CRISPR endonuclease Cas9 in mammalian cells

(2014) Xuebing Wu et al.   NATURE BIOTECHNOLOGY

Simultaneous editing of three homoeoalleles in hexaploid bread wheat confers heritable resistance to powdery mildew

(2014) Yanpeng Wang et al.   NATURE BIOTECHNOLOGY

Efficient CRISPR-Cas9–mediated genome editing in Plasmodium falciparum

(2014) Jeffrey C Wagner et al.   NATURE METHODS

CRISPR/Cas-mediated genome editing in the rat via direct injection of one-cell embryos

(2014) Yanjiao Shao et al.   Nature Protocols

Large chromosomal deletions and heritable small genetic changes induced by CRISPR/Cas9 in rice

(2014) Huanbin Zhou et al.   NUCLEIC ACIDS RESEARCH

Efficient Gene Editing in Tomato in the First Generation Using the Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR-Associated9 System

(2014) C. Brooks et al.   PLANT PHYSIOLOGY

Targeted Mutagenesis in Atlantic Salmon (Salmo salar L.) Using the CRISPR/Cas9 System Induces Complete Knockout Individuals in the F0 Generation

(2014) Rolf B. Edvardsen et al.   PLoS One

Efficient Gene Knockout in Goats Using CRISPR/Cas9 System

(2014) Wei Ni et al.   PLoS One

Heritable Genome Editing with CRISPR/Cas9 in the Silkworm, Bombyx mori

(2014) Wei Wei et al.   PLoS One

Efficient in vivo deletion of a large imprinted lncRNA by CRISPR/Cas9

(2014) Jinxiong Han et al.   RNA Biology

Understanding functional miRNA–target interactions in vivo by site-specific genome engineering

(2014) Andrew R. Bassett et al.   Nature Communications

One-Step Generation of Mice Carrying Mutations in Multiple Genes by CRISPR/Cas-Mediated Genome Engineering

(2013) Haoyi Wang et al.   CELL

Dynamic Imaging of Genomic Loci in Living Human Cells by an Optimized CRISPR/Cas System

(2013) Baohui Chen et al.   CELL

Efficient Gene Targeting in Zebrafish Mediated by a Zebrafish-Codon-Optimized Cas9 and Evaluation of Off-Targeting Effect

(2013) Da Liu et al.   Journal of Genetics and Genomics

DNA targeting specificity of RNA-guided Cas9 nucleases

(2013) Patrick D Hsu et al.   NATURE BIOTECHNOLOGY

Orthogonal Cas9 proteins for RNA-guided gene regulation and editing

(2013) Kevin M Esvelt et al.   NATURE METHODS

Genetic Screens in Human Cells Using the CRISPR-Cas9 System

(2013) Tim Wang et al.   SCIENCE

Multiplex Genome Engineering Using CRISPR/Cas Systems

(2013) L. Cong et al.   SCIENCE

An integrated encyclopedia of DNA elements in the human genome

(2012)   NATURE

A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity

(2012) M. Jinek et al.   SCIENCE

CRISPR RNA maturation by trans-encoded small RNA and host factor RNase III

(2011) Elitza Deltcheva et al.   NATURE

Enzymatic assembly of DNA molecules up to several hundred kilobases

(2009) Daniel G Gibson et al.   NATURE METHODS

Ultrafast and memory-efficient alignment of short DNA sequences to the human genome

(2009) Ben Langmead et al.   GENOME BIOLOGY