CRISPR/Cas9-mediated gene knockout in the ascidianCiona intestinalis

Efficient RNA/Cas9-mediated genome editing in Xenopus tropicalis

(2014) X. Guo et al.   DEVELOPMENT

Nuclease-mediated genome editing: At the front-line of functional genomics technology

(2014) Tetsushi Sakuma et al.   DEVELOPMENT GROWTH & DIFFERENTIATION

Germ cell mutations of the ascidianCiona intestinaliswith TALE nucleases

(2014) Keita Yoshida et al.   GENESIS

Efficient genome modification by CRISPR-Cas9 nickase with minimal off-target effects

(2014) Bin Shen et al.   NATURE METHODS

Efficient genome editing in plants using a CRISPR/Cas system

(2013) Zhengyan Feng et al.   CELL RESEARCH

Tissue-specific and ubiquitous gene knockouts by TALEN electroporation provide new approaches to investigating gene function in Ciona

(2013) N. Treen et al.   DEVELOPMENT

Efficient CRISPR/Cas9 genome editing with low off-target effects in zebrafish

(2013) A. Hruscha et al.   DEVELOPMENT

Versatile strategy for isolating transcription activator-like effector nuclease-mediated knockout mutants inCaenorhabditis elegans

(2013) Takuma Sugi et al.   DEVELOPMENT GROWTH & DIFFERENTIATION

TALEN-induced gene knock out inDrosophila

(2013) Takefumi Kondo et al.   DEVELOPMENT GROWTH & DIFFERENTIATION

Targeted mutagenesis in sea urchin embryos using TALENs

(2013) Sayaka Hosoi et al.   DEVELOPMENT GROWTH & DIFFERENTIATION

Targeted mutagenesis of multiple and paralogous genes inXenopus laevisusing two pairs of transcription activator-like effector nucleases

(2013) Yuto Sakane et al.   DEVELOPMENT GROWTH & DIFFERENTIATION

Design, evaluation, and screening methods for efficient targeted mutagenesis with transcription activator-like effector nucleases in medaka

(2013) Satoshi Ansai et al.   DEVELOPMENT GROWTH & DIFFERENTIATION

Gene targeting technologies in rats: Zinc finger nucleases, transcription activator-like effector nucleases, and clustered regularly interspaced short palindromic repeats

(2013) Tomoji Mashimo   DEVELOPMENT GROWTH & DIFFERENTIATION

Recent progress in genome engineering techniques in the silkworm,Bombyx mori

(2013) Takaaki Daimon et al.   DEVELOPMENT GROWTH & DIFFERENTIATION

Transcription activator-like effector nucleases efficiently disrupt the target gene in Iberian ribbed newts (Pleurodeles waltl), an experimental model animal for regeneration

(2013) Toshinori Hayashi et al.   DEVELOPMENT GROWTH & DIFFERENTIATION

Genome editing using artificial site-specific nucleases in zebrafish

(2013) Yu Hisano et al.   DEVELOPMENT GROWTH & DIFFERENTIATION

Feasibility for a large scale mouse mutagenesis by injecting CRISPR/Cas plasmid into zygotes

(2013) Daisuke Mashiko et al.   DEVELOPMENT GROWTH & DIFFERENTIATION

Efficient TALEN construction and evaluation methods for human cell and animal applications

(2013) Tetsushi Sakuma et al.   GENES TO CELLS

Simple and efficient CRISPR/Cas9-mediated targeted mutagenesis inXenopus tropicalis

(2013) Takuya Nakayama et al.   GENESIS

Genome Engineering of Drosophila with the CRISPR RNA-Guided Cas9 Nuclease

(2013) Scott J. Gratz et al.   GENETICS

Efficient Targeted Mutagenesis in Medaka Using Custom-Designed Transcription Activator-Like Effector Nucleases

(2013) S. Ansai et al.   GENETICS

Highly Improved Gene Targeting by Germline-Specific Cas9 Expression in Drosophila

(2013) Shu Kondo et al.   GENETICS

RNA-Guided Genome Editing in Plants Using a CRISPR–Cas System

(2013) Kabin Xie et al.   Molecular Plant

Efficient genome editing in zebrafish using a CRISPR-Cas system

(2013) Woong Y Hwang et al.   NATURE BIOTECHNOLOGY

High-frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells

(2013) Yanfang Fu et al.   NATURE BIOTECHNOLOGY

Cas9 as a versatile tool for engineering biology

(2013) Prashant Mali et al.   NATURE METHODS

Heritable genome editing in C. elegans via a CRISPR-Cas9 system

(2013) Ari E Friedland et al.   NATURE METHODS

Repeating pattern of non-RVD variations in DNA-binding modules enhances TALEN activity

(2013) Tetsushi Sakuma et al.   Scientific Reports

High efficiency TALENs enable F0 functional analysis by targeted gene disruption in Xenopus laevis embryos

(2013) K.-i. T. Suzuki et al.   Biology Open

Retinoic acid-driven Hox1 is required in the epidermis for forming the otic/atrial placodes during ascidian metamorphosis

(2012) Y. Sasakura et al.   DEVELOPMENT

Efficient targeted mutagenesis of the chordate Ciona intestinalis genome with zinc-finger nucleases

(2012) Narudo Kawai et al.   DEVELOPMENT GROWTH & DIFFERENTIATION

RNA-guided genetic silencing systems in bacteria and archaea

(2012) Blake Wiedenheft et al.   NATURE

Zinc-finger nuclease-mediated targeted insertion of reporter genes for quantitative imaging of gene expression in sea urchin embryos

(2012) H. Ochiai et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Non-transgenic genome modifications in a hemimetabolous insect using zinc-finger and TAL effector nucleases

(2012) Takahito Watanabe et al.   Nature Communications

Efficient design and assembly of custom TALEN and other TAL effector-based constructs for DNA targeting

(2011) Tomas Cermak et al.   NUCLEIC ACIDS RESEARCH

Targeting DNA Double-Strand Breaks with TAL Effector Nucleases

(2010) M. Christian et al.   GENETICS

A TALE nuclease architecture for efficient genome editing

(2010) Jeffrey C Miller et al.   NATURE BIOTECHNOLOGY

ZiFiT (Zinc Finger Targeter): an updated zinc finger engineering tool

(2010) J. D. Sander et al.   NUCLEIC ACIDS RESEARCH

Maternal factor-mediated epigenetic gene silencing in the ascidian Ciona intestinalis

(2009) Yasunori Sasakura et al.   MOLECULAR GENETICS AND GENOMICS

RNA interference by expressing short hairpin RNA in theCiona intestinalisembryo

(2008) Aya Nishiyama et al.   DEVELOPMENT GROWTH & DIFFERENTIATION

Targeted gene knockout in mammalian cells by using engineered zinc-finger nucleases

(2008) Y. Santiago et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations

(2008) Yutaka Satou et al.   GENOME BIOLOGY