CRIMoClo plasmids for modular assembly and orthogonal chromosomal integration of synthetic circuits in Escherichia coli

Absolute quantification of translational regulation and burden using combined sequencing approaches

(2019) Thomas E Gorochowski et al.   Molecular Systems Biology

Deconvolution of Luminescence Cross-Talk in High-Throughput Gene Expression Profiling

(2019) Marco Mauri et al.   ACS Synthetic Biology

Burden-driven feedback control of gene expression

(2018) Francesca Ceroni et al.   NATURE METHODS

Engineering orthogonal synthetic timer circuits based on extracytoplasmic function σ factors

(2018) Daniela Pinto et al.   NUCLEIC ACIDS RESEARCH

Mobius Assembly: A versatile Golden-Gate framework towards universal DNA assembly

(2018) Andreas I. Andreou et al.   PLoS One

Loop assembly: a simple and open system for recursive fabrication of DNA circuits

(2018) Bernardo Pollak et al.   NEW PHYTOLOGIST

Start-Stop Assembly: a functionally scarless DNA assembly system optimized for metabolic engineering

(2018) George M Taylor et al.   NUCLEIC ACIDS RESEARCH

Systematic characterization of maturation time of fluorescent proteins in living cells

(2017) Enrique Balleza et al.   NATURE METHODS

Resource Competition Shapes the Response of Genetic Circuits

(2017) Yili Qian et al.   ACS Synthetic Biology

Effect of Genomic Integration Location on Heterologous Protein Expression and Metabolic Engineering in E. coli

(2017) Jacob A. Englaender et al.   ACS Synthetic Biology

A Family of Single Copy repABC-Type Shuttle Vectors Stably Maintained in the Alpha-Proteobacterium Sinorhizobium meliloti

(2017) Johannes Döhlemann et al.   ACS Synthetic Biology

Lambda Red recombinase-mediated integration of the high molecular weight DNA into the Escherichia coli chromosome

(2016) Mario Juhas et al.   Microbial Cell Factories

Complex transcriptional modulation with orthogonal and inducible dCas9 regulators

(2016) Yuchen Gao et al.   NATURE METHODS

Transcription-coupled DNA supercoiling dictates the chromosomal arrangement of bacterial genes

(2016) Patrick Sobetzko   NUCLEIC ACIDS RESEARCH

Design and Assembly of DNA Sequence Libraries for Chromosomal Insertion in Bacteria Based on a Set of Modified MoClo Vectors

(2016) Daniel Schindler et al.   ACS Synthetic Biology

Bricks and blueprints: methods and standards for DNA assembly

(2015) Arturo Casini et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology

(2015) Sonya V. Iverson et al.   ACS Synthetic Biology

Escherichia coli Flagellar Genes as Target Sites for Integration and Expression of Genetic Circuits

(2014) Mario Juhas et al.   PLoS One

Rapid and Reliable DNA Assembly via Ligase Cycling Reaction

(2014) Stefan de Kok et al.   ACS Synthetic Biology

Knock-in/Knock-out (KIKO) vectors for rapid integration of large DNA sequences, including whole metabolic pathways, onto the Escherichia coli chromosome at well-characterised loci

(2013) Suriana Sabri et al.   Microbial Cell Factories

Design of orthogonal genetic switches based on a crosstalk map of  s, anti- s, and promoters

(2013) V. A. Rhodius et al.   Molecular Systems Biology

Genomic mining of prokaryotic repressors for orthogonal logic gates

(2013) Brynne C Stanton et al.   Nature Chemical Biology

Characterization of 582 natural and synthetic terminators and quantification of their design constraints

(2013) Ying-Ja Chen et al.   NATURE METHODS

One-Step Cloning and Chromosomal Integration of DNA

(2013) François St-Pierre et al.   ACS Synthetic Biology

Fast track assembly of multigene constructs using Golden Gate cloning and the MoClo system

(2012) Stefan Werner et al.   Bioengineered

Roles of Two Shewanella oneidensis MR-1 Extracellular Endonucleases

(2011) Julia Gödeke et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

DNA assembly for synthetic biology: from parts to pathways and beyond

(2011) Tom Ellis et al.   Integrative Biology

A Modular Cloning System for Standardized Assembly of Multigene Constructs

(2011) Ernst Weber et al.   PLoS One

Factors affecting plasmid production in Escherichia coli from a resource allocation standpoint

(2009) Drew S Cunningham et al.   Microbial Cell Factories

Enzymatic assembly of DNA molecules up to several hundred kilobases

(2009) Daniel G Gibson et al.   NATURE METHODS

Golden Gate Shuffling: A One-Pot DNA Shuffling Method Based on Type IIs Restriction Enzymes

(2009) Carola Engler et al.   PLoS One